共被期刊论文引用了2次
您的检索式：您选中1篇文献正在查看引证文献汇总
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | NADPH氧化酶在TGF-β_1诱导大鼠小管上皮细胞表达炎症因子中的作用显示文摘【目的】观察转化生长因子β1(TGF-β1)对大鼠肾小管上皮细胞单核细胞趋化因子-1(MCP-1)及白细胞介素-6(IL-6)表达的影响,并探讨NADPH氧化酶在其中的作用。【方法】以大鼠肾小管上皮细胞株(NRK-52E)为研究对象,采用TGF-β1(10ng/mL)刺激0h,2h,4h,8h,12h,24h分别收取RNA;刺激0h,12h,24h,48h,72h分别收取细胞上清液;部分实验中培养的细胞在刺激前用NADPH氧化酶抑制剂DPI(0.1,1,5,10μmol/L)预处理1h,然后含10ng的TGF-β1无血清DMEM/F12培养液分别培养12h(收集RNA)和24h(收集细胞上清液)。所有实验重复3次。细胞内活性氧(ROS)的产生采用激光共聚焦显微镜观察。NADPH氧化酶p22phox、gp91phox、p47phox和p67phox亚单位以及MCP-1和IL-6mRNA的表达采用RT-PCR检测。细胞上清液中MCP-1的含量采用ELISA检测。【结果】TGF-β1可显著上调NADPH氧化酶p67phox亚单位mRNA的表达,8h为对照的2.43倍(P<0.01),24h达3.59倍(P<0.01)。但对p22phox、gp91phox和p47phox亚单位mRNA表达无显著影响。TGF-β1可显著增加细胞内ROS产生,DPI预处理后ROS产生较TGF-β1刺激组降低了43.69%(P<0.05)。TGF-β1可显著上调MCP-1和IL-6mRNA及蛋白的表达。DPI预处理可明显逆转TGF-β1诱导的MCP-1和IL-6的上调表达。【结论】TGF-β1可促使细胞ROS产生增加。TGF-β1可能通过NADPH氧化酶依赖的ROS介导了大鼠肾小管上皮细胞MCP-1及IL-6的上调表达。 | 张海燕 姜宗培 常洁 李晓艳 朱恒梅 余学清 | 2008 | 中山大学学报(医学科学版)2008，29，3： | 3 |
| 2 | Late recurrence after surgery for cholangiocarcinoma:implications for follow-up?显示文摘BACKGROUND:Biliary tract cancer is uncommon,but has a high rate of early recurrence and a poor prognosis. There is only limited information on patients surviving more than 5 years after resection. METHODS:We report a patient who developed recurrence 8 years after resection of cholangiocarcinoma.Descriptions of late recurrence after excision of cholangiocarcinoma are reviewed. RESULTS:Few long-term survivors with biliary tract cancer have been reported.The survivors tend to have well differentiated or papillary tumors.The present case had no recurrence for 8 years despite poor prognostic factors including poor differentiation,invasion through the muscle wall and perineural invasion.It has been suggested that tumor cells left after the first operation grow and present as late recurrence.There is a need to differentiate a new primary and field change from recurrence of the previous tumor. CONCLUSIONS:Long-term follow-up after resection of cholangiocarcinoma is needed because late recurrence after 5 years occurs.The mortality rate between 5 and 10 years after resection of cholangiocarcinoma ranges from 6%to 43%in different series.Early detection of local recurrence may give an opportunity for further surgical resection. | Harun Thomas Nigel David Heaton | 2008 | Hepatobiliary & Pancreatic Diseases International2008，7，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员