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| 1 | 栉孔扇贝热休克蛋白70(HSP70)基因的BAC-FISH定位显示文摘利用BAC-FISH技术,将包含HSP70基因的BAC克隆定位到栉孔扇贝的一对同源染色体的长臂上。HSP70基因的染色体定位将对深入研究该基因的结构及功能并将其应用于生产实践提供基础支持。同时,本研究是首次对栉孔扇贝低拷贝基因进行染色体定位的实践,其结果将为栉孔扇贝的染色体的深入研究以及染色体鉴别等工作提供必要的参考。 | 郇聘 张晓军 李富花 张洋 赵翠 刘保忠 相建海 | 2009 | 海洋科学2009，33，7： | 4 |
| 2 | 薄片牡蛎的核型及18S-28S核糖体rRNA基因的染色体定位显示文摘以薄片牡蛎(Dendostrea folium)成体鳃组织为材料制备有丝分裂中期染色体标本,对其染色体核型进行了分析,并运用荧光原位杂交技术(FISH)将18S-28S核糖体RNA基因定位于中期染色体上。FISH探针是通过PCR扩增介于18S-28S rRNA基因之间的ITS和5.8S rRNA基因序列,并在PCR扩增过程中掺入了Biotin-11-dUTP进行生物素标记。结果显示,薄片牡蛎的单倍染色体数目为n=10,全部为中部着丝粒染色体。与大多数已知巨蛎属牡蛎的染色体核型相似。ITS探针在薄片牡蛎中期分裂体相上产生两簇FISH信号,分别杂交于2号染色体短臂的近端粒区域。本研究首次报道了薄片牡蛎的中期染色体核型以及18S-28S核糖体RNA基因在染色体上的定位。 | 赵文溪 张楠 王嫣 石耀华 顾志峰 王爱民 | 2012 | 基因组学与应用生物学2012，31，1： | 3 |
| 3 | A populational survey of 45S rDNA polymorphism in the Jefferson salamander Ambystoma jeffersonianum revealed by fluorescence in situ hybridization(FISH)显示文摘The chromosomal localization of 45S ribosomal RNA genes in Ambystoma jeffersonianum was determined by fluorescence in situ hybridization with 18S rDNA fragment as a probe (FISH-rDNA). Our results revealed the presence of rDNA polymorphism among A.jeffersonianum populations in terms of number,location and FISH signal intensity on the chromosomes. Nine rDNA cytotypes were found in ten geographically isolated populations and most of them contained derivative rDNA sites. Our preliminary study provides strong indication of karyotypic diversification of A.jeffersonianum that is demonstrated by intraspecific variation of 45S rDNA cytotypes. rDNA cytotype polymorphism has been described in many other caudate amphibians. We predict that habitat isolation,low dispersal ability and decline of effective population size could facilitate the fixation and accumulation of variable rDNA cytotypes during their chromosome evolution. | James P. BOGART | 2009 | Current Zoology2009，55，2： | 2 |
| 4 | 一种栉孔扇贝丝氨酸蛋白酶基因的染色体定位及其内部SNP的研究显示文摘病害问题是制约我国扇贝养殖业发展的关键,因此贝类的先天免疫也成为当前研究的热点。丝氨酸蛋白酶是先天免疫中至关重要的酶类,在许多通路中起信号放大作用。目前对栉孔扇贝丝氨酸蛋白酶的研究主要集中于基因序列分析和表达谱研究,基因定位方面的研究尚未开展。文章以包含一种栉孔扇贝丝氨酸蛋白酶基因的BAC克隆为探针,利用BAC-FISH技术将其定位到一对同源染色体的长臂上,为丝氨酸蛋白酶基因的后续研究提供了基础;同时,利用PCR产物直接测序法筛选了该基因内部的6个SNP标记,这些SNP标记可供遗传图谱定位使用,从而可以实现遗传图谱与染色体间的锚定与初步整合。 | 郇聘 张晓军 李富花 张洋 赵翠 刘保忠 相建海 | 2009 | 遗传2009，31，12： | 2 |
| 5 | Characterization, Expression and Function Analysis of DAX1 Gene of Scallop(Chlamys farreri Jones and Preston 1904) During Its Gametogenesis显示文摘DAX1, a member of nuclear receptor superfamily, has a function in the sex determination and gonadal differentiation of several vertebrate species. However, little information about DAX1 of invertebrates is available. Here we cloned a homolog of scallop(Chlamys farreri Jones and Preston 1904) dax1, Cf-dax1, and determined its expression characteristics at m RNA and protein levels. The c DNA sequence of Cf-dax1 was 2093 bp in length, including 1404 bp open reading frame(ORF) encoding 467 amino acids. Unlike those of vertebrates, no conserved LXXLL-related motif was found in the putative DNA binding region of Cf-DAX1. Fluorescence in situ hybridization showed that Cf-dax1 located on the short arm of a pair of subtelocentric chromosomes. Tissue distribution analysis using semi-quantitative RT-PCR revealed that Cf-dax1 expressed widely in adult scallop tissues, with the highest expression level found in adductor muscle, moderate level in mantle, gill and testis, and low level in kidney, ovary and hepatopancreas. The result of quantitative real-time PCR indicated that the expression of Cf-dax1 was significantly higher(P<0.05) in testis than in ovary at the same stage, showing a sex-dimorphic expression pattern. Furthermore, immunohistochemical detection found that Cf-DAX1 mainly located in spermatogonia and spermatocytes of testis and in oogonia and oocytes of ovary, implying that DAX1 may involve in gametogenesis of bivalves. | LI Hailong LIU Jianguo HUANG Xiaoting WANG Dan ZHANG Zhifeng | 2014 | Journal of Ocean University of China2014，13，4： | 2 |
| 6 | 栉孔扇贝DAPI带型和PI带型研究显示文摘选取栉孔扇贝Chlamys farreri担轮幼虫为材料,采用秋水仙素-低渗-空气干燥法制备染色体标本,应用荧光显带技术,分析了DAPI带和PI带在栉孔扇贝染色体上的分布。DAPI带型结果显示,栉孔扇贝所有染色体上都存在DAPI阳性带,主要分布于传统的着丝粒区和端部区域,另外还存在一些中间区DAPI带及可变带,总带数为62。PI带型结果与DAPI带型结果相似,在所有染色体上都存在PI阳性带。2种带型的阳性带所在位置与异染色质分布区域相吻合。 | 徐俊 包振民 任晓亮 王珊 胡丽萍 黄晓婷 | 2011 | 中国海洋大学学报(自然科学版)2011，41，4： | 2 |
| 7 | Cytogenetic analysis in two scallops (Bivalvia:Pectinidae) by PRINS and PI banding显示文摘Cytogenetic analysis was performed for the bay scallop(Argopecten irradians Lamarck 1819) and the Japanese scallop(Patinopecten yessoensis Jay 1857) by primed in situ labeling(PRINS) and propidium iodide(PI) banding techniques.The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A.irradians and on two submetacentric pairs in P.yessoensis.The histone H3 gene sites differed in number and location between these two species.The C-band-like patterns revealed by PI staining varied considerably between these two species.A.irradians displayed terminal bands at long arms on all chromosomes,centromeric bands on some pairs and interstitial bands on five pairs.P.yessoensis exhibited only centromeric bands on all chromosomes.These results would contribute to the better understanding of karyotype evolution in A.irradians and P.yessoensis. | ZHANG Lingling BAO Zhenmin WANG Jue WANG Shi HUANG Xiaoting HU Xiaoli HU Jingjie | 2007 | Acta Oceanologica Sinica2007，26，5： | 2 |
| 8 | 栉孔扇贝NDPK基因的BAC-FISH定位研究显示文摘栉孔扇贝(Chlamys farreri)是我国北部沿海一个非常重要的养殖种类。核苷二磷酸激酶(NDPK)是一类广泛存在、高度保守,在细胞能量和信息传递中具有重要作用的酶。作者利用BAC-FISH技术将两个包含栉孔扇贝NDPK基因的BAC克隆定位到染色体同一位置上,该结果验证了物理图谱相关contig组装的可靠性,同时NDPK基因的染色体定位将对深入研究该基因的结构、功能以及应用于生产实践提供理论支撑。本研究结果将对栉孔扇贝染色体的深入研究以及染色体鉴别、图谱整合等工作提供重要参考。 | 黄超 郇聘 张晓军 相建海 | 2012 | 海洋科学2012，36，1： | 2 |
| 9 | Development of an in situ loop-mediated isothermal amplification technique for chromosomal localization of DNA sequences显示文摘In situ loop-mediated isothermal amplification (in situ LAMP) combines in situ hybridization and loop-mediated isothermal amplification (LAMP) techniques for chromosomal localization of DNA sequences. In situ LAMP is a method that is generally more specific and sensitive than conventional techniques such as fluorescence in situ hybridization (FISH), primed in situ labeling (PRINS), and cycling primed in situ labeling (C-PRINS). Here, we describe the development and application of in situ LAMP to identify the chromosomal localization of DNA sequences. To benchmark this technique, we successfully applied this technique to localize the major ribosomal RNA gene on the chromosomes of the Zhikong scallop (Chlamys farreri). | 孟庆磊 王师 张玲玲 黄晓婷 包振民 | 2013 | Chinese Journal of Oceanology and Limnology2013，31，1： | 1 |
| 10 | 扇贝核糖体ITS1和5SrDNA序列的遗传变异及亲缘关系分析显示文摘为探讨引进种岩扇贝(Crassadoma gigantea)及虾夷扇贝(Patinopecten yessoensis)、栉孔扇贝(Chlamys farreri)和海湾扇贝(Argopecten irradians)的遗传变异及其之间的亲缘关系,研究对4种扇贝的核糖体ITS1序列及5S rDNA序列进行了测定,获得了4种扇贝的ITS1和5S rDNA全序列以及18S rDNA的部分序列,并进行了序列特征、遗传多样性、遗传距离及系统发育等相关分析。结果显示,基于ITS1序列的单倍型数、核苷酸变异位点、单倍型多样性指数、核苷酸多样性指数及平均核苷酸差异数分别在2—6、1—12、0.425—0.800、0.00081—0.00486和0.366—2.442;基于5S序列的相应参数分别在2—5、1—14、0.533—0.841、0.00108—0.01058和0.533—5.726。4种扇贝均表现出较低的多样性水平(π<0.01)。此外,4种扇贝基于2种方法计算的种间遗传距离分别在0.040—0.121和0.042—0.413,均表现为岩扇贝与虾夷扇贝的遗传距离最近,而岩扇贝与海湾扇贝遗传距离最远。并且系统进化树分析显示岩扇贝与虾夷扇贝独自聚为一支,说明引进物种岩扇贝与虾夷扇贝的亲缘关系较近。研究结果将为岩扇贝的种质资源保护以及今后的遗传育种研究工作提供参考信息。 | 廖德杰 曹善茂 童金苟 周颖 俞小牧 刘阳 王潇 | 2018 | 水生生物学报2018，42，3： | 1 |
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