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| 1 | 转基因大豆安全性评价的研究进展显示文摘自从1996年第1个转基因抗草甘膦大豆品种商业化种植以来,转基因大豆在世界范围内迅速推广。转基因大豆的种植在带来巨大经济效益的同时,食品和环境安全性的潜在风险受到广泛关注。本文综述转基因大豆的优势、食品及环境安全性,为我国转基因大豆的商品化提供参考。 | 左娇 郭运玲 孔华 徐林 周霞 郭安平 | 2013 | 热带作物学报2013，34，7： | 5 |
| 2 | Sexual compatibility of transgenic soybean and different wild soybean populations显示文摘The introduction of genetically modified(GM) soybean into farming systems raises great concern that transgenes from GM soybean may flow to endemic wild soybean via pollen. This may increase the weediness of transgenic soybean by increasing the fitness of hybrids under certain conditions and threaten the genetic diversity of wild soybean populations. Although pollen-mediated gene flow between GM crops and wild relatives is dependent on many factors, the sexual compatibility(SC)determined by their genetic backgrounds is the conclusive factor. The considerable genetic variation among wild soybean populations may cause compatibility differences between different wild and cultivated soybeans. Thus, an evaluation of the SC between transgenic soybean and different wild soybeans is essential for assessing the environmental consequences of cultivated soybean–wild soybean transgene flow. The podding and seed sets were assessed after artificial hybridization using transgenic glyphosate-resistant soybean as the paternal parent and 18 wild soybean populations as the maternal parents. Then, the average number of filled seeds produced in 200 flowers(AFS) was calculated for each wild soybean under natural self-pollination as well as under artificial crossing with transgenic soybean. Finally, the index of cross-SC was calculated(ICSC) as the ratio of the AFS of wild soybean artificially crossed with transgenic soybean and the AFS of naturally self-pollinated wild soybean. The results demonstrated that after self-pollination and crossing with transgenic soybean, the average podding rates of 18 wild soybean populations ranged within 96.50–99.50% and 4.92–18.03%, and the average filled seed numbers per pod varied from 1.70 to 2.69 and 0.20 to 0.48, respectively. The results showed that approximately 89% of wild soybeans displayed either medium or higher than medium SC with transgenic soybean(ICSC>1.0%). This implied the high possibility of gene flow via pollen from transgenic soybean to wild soybean. | HU Yu-qi SHENG Ze-wen LIU Jin-yue LIU Qi QIANG Sheng SONG Xiao-ling LIU Biao | 2022 | Journal of Integrative Agriculture2022，21，1： | 1 |
| 3 | 抗草甘膦转基因大豆与野大豆杂交F_(3)代表型性状分析显示文摘为了评估转基因大豆向野大豆(Glycine soja Sieb.et Zucc.)基因漂移后可能产生的生态风险,选取抗草甘膦转基因大豆(携带外源基因cp4-epsps)与野大豆(来自内蒙古包头)杂交F_(2)代种皮颜色为黑色、褐绿色和褐黄色(与野大豆种皮颜色相近)的种子,对这些种子萌发产生的F_(3)代植株的19个定量性状和5个定性性状进行分析,在此基础上,将F_(3)代植株及其亲本进行聚类分析,并对不同组F_(3)代的定量和定性性状进行分析。结果显示:F_(3)代幼苗中抗性植株与非抗性植株的分离比符合“5∶1”的孟德尔遗传定律。定量性状中,结实率的变异系数最小(4.65%),其余18个性状的变异系数高于14%,其中,单株种子数的变异系数最大(55.68%)。不同种皮颜色F_(2)代种子萌发产生的F_(3)代植株均以强缠绕型占比最高;F_(3)代植株中,90.7%的果荚为弯镰形或弓形,且80.0%的果荚颜色为黑褐色;黑色种皮F_(2)代种子萌发产生的F_(3)代种皮颜色无性状分离,褐绿色和褐黄色种皮F_(2)代种子萌发产生的F_(3)代种皮颜色均出现性状分离;F_(3)代种子中,82.7%的种子有泥膜。聚类结果显示F_(3)代植株被分为3组。不同组F_(3)代中,Ⅰ组的多数性状最低,且株高等5个性状显著低于野大豆;Ⅱ组的9个性状最高,且单株结荚数等15个性状显著高于野大豆,仅株高显著低于野大豆;Ⅲ组的部分性状最高,且单株结荚数等12个性状显著高于野大豆,株高等3个性状显著低于野大豆。综合比较认为,Ⅱ组的竞争能力最强,Ⅰ组的竞争能力最弱。Ⅰ组的不缠绕型植株占比最高,而Ⅱ和Ⅲ组的强缠绕型植株占比最高。3组F_(3)代的种皮颜色均以黑色占比最高。综上所述,供试的不同组F_(3)代植株均能完成生活史并产生后代。若抗草甘膦转基因大豆的基因通过花粉漂移到野大豆上并成功杂交,则会产生不同适应性杂交后代,增加生态风险。 | 刘佳丽 刘金悦 纪雪勤 强胜 宋小玲 | 2023 | 植物资源与环境学报2023，32，2： | 0 |
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