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| 1 | Profiling of the transcriptome of Porphyra yezoensis with Solexa sequencing technology显示文摘With high-throughput Solexa sequencing technology,we profiled Porphyra yezoensis transcriptomes from 8 different samples. More than 1200 megabases from 13333334 quality paired-end reads were generated,which were assembled into 31538 unigenes. Blast analysis showed that 56.7%unigenes were novel,which represented the specific genes of Porphyra and/or rhodophytes. Several hundreds of unigenes related to stress tolerance were discovered,including genes related to desiccation-(211) and high light-tolerance(31) ,flavonoid biosynthesis(10) ,reactive oxygen scavenging(48) and other stress-tolerance processes(208) ,which indicated there existed complex and diversity modes of stress tolerance in this species.A complete set of essential genes involved in the C3-(57) and C4-(44) carbon fixation pathway(except pyruvate phosphate dikinase) were discovered,which not only proved that they were actively transcribed but also clearly outlined the panoptic view of carbon fixation in Porphyra.Moreover,by statistically analyzing the types,proportions and frequencies of the interspersed repeats(TEs) and simple sequence repeats(SSRs) ,we discovered that the top three types of TEs were all retrotransposons and the trinucleotide was the absolute predominant type among SSRs,promoting our understanding of structural characteristics of the transcriptome.This study substantially improved the global view of the Porphyra genome and provided a valuable resource for future research. | YANG Hui MAO YunXiang KONG FanNa YANG GuanPin MA Fei WANG Li | 2011 | Chinese Science Bulletin2011，56，20： | 13 |
| 2 | Cloning and Analysis of Calmodulin Gene from Porphyra yezoensis Ueda (Bangiales, Rhodophyta)显示文摘In order to understand the mechanisms of signal transduction and anti-desiccation mechanisms of Porphyra yezoensis, cDNA and its genomic sequence of Calmodulin gene (CaM) was cloned by the technique of polymerase chain reaction (PCR) based on the analysis of P. yezoensis ESTs from dbEST database. The result shows that the full-length cDNA of CaM consists of 603 bps including an ORF encoding for 151 amino acids and a terminate codon UGA, while the length of genomic sequence is 1231 bps including 2 exons and 1 intron. The average GC content of the coding region is 58.77%, while the GC content of the third position of this gene is as high as 82.23%. Four Ca2+ binding sites (EF-hand) are found in this gene. The predicted molecular mass of the deduced peptide is 16688.72 Da and the pI is 4.222. By aligning with known CaM genes, the similarity of CaM gene sequence with homologous genes in Chlamydomonas incerta and Chlamydomonas reinhardtii is 72.7% and 72.2% respectively, and the similarity of the deduced amino acid sequence of CaM gene with homologous genes in C. incerta and C. reinhardtii are both 71.5%. This is the first report on CaM from a species of Rhodophyta. | WANG Mengqiang MAO Yunxiang ZHUANG Yunyun KONG Fanna SUI Zhenghong | 2009 | Journal of Ocean University of China2009，8，3： | 3 |
| 3 | 甘紫菜细胞色素c_6基因petJ克隆与序列分析显示文摘以野生甘紫菜为材料,采用同源克隆技术获得了细胞色素c_6基因petJ的全长序列,由此推导出相应的氨基酸序列,并与部分藻类的同源基因进行了相似性和同源性分析。结果表明:由核苷酸序列推导的氨基酸序列中,包含10个碱性氨基酸残基,13个酸性氨基酸残基,46个巯水性氨基酸残基和27个极性氨基酸残基。甘紫菜(Porphyra tenera Kjellm)pet J基因编码区核苷酸序列和蛋白序列与条斑紫菜的相似性最高(99.0%,99.0%),其次是Porphyra purpurea(88.5%,92.7%)。而与其他藻类中的同源序列相似性较低。利用该基因在甘紫菜和条斑紫菜的差异位点可以开发用于区别这两种紫菜的分子标记。该基因的系统发生结果与内共生学说一致,可能是内共生学说在分子水平上的1个体现。 | 王孟强 茅云翔 李杰 张蕾 刘玮 庄昀筠 | 2007 | 中国海洋大学学报(自然科学版)2007，37，S1： | 2 |
| 4 | 浅谈企业多元化战略下的财务策略显示文摘一、相关概述伴随着世界经济一体化进程的不断加快以及我国社会主义市场经济体制改革的不断深化.企业所面临的内外经营环境都发生了重大变化.企业在日常经营管理过程中需要解决的风险和不确定因素也越来越多.对于企业的管理科学化水平提出了更高的要求。正是在这一大背景下.不少企业积极实施多元化经营战略. | 张译文 | 2014 | 中国集体经济2014，，13： | 1 |
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