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| 1 | Tumor necrosis factor-alpha -308G/A polymorphism and risk of hepatocellular carcinoma in hepatitis C virus-infected patients显示文摘Tumor necrosis factor-alpha (TNF-α) is an important cytokine in generating an immune response against infection with hepatitis C virus (HCV). The functions of TNF-α may be altered by single-nucleotide polymorphisms (SNPs) in its gene structure. We hypothesized that SNPs in TNF-α may be important in determining the outcome of an HCV infection. To test this hypothesis, we investigated the role of the polymorphism -308G/A, which is located in the promoter region of the TNF-α gene, in the progression of HCV infection in Egyptian patients using a quantitative real-time polymerase chain reaction (qRT-PCR). The distribution of this polymorphism and its impact on the serum level of TNF-α was compared between 90 HCV-infected patients [45 with HCV-induced cirrhosis and 45 with HCV-related hepatocellular carcinoma (HCC)] and 45 healthy Egyptian volunteers without any history of liver disease. Our results showed that at the TNF-α -308 position, the G/G allele was most common (78.5% ) in the study population, with the G/A and A/A alleles occurring less frequently (13.3% and 8.1% , respectively). Frequencies of G/G, G/A, and A/A genotypes were 87%, 7%, and 6% in patients with liver cirrhosis and were 94% , 4% , and 2% in patients with HCC, respectively. Serum levels of TNF-α were significantly higher in HCV-infected patients than in healthy controls, indicating that the TNF-α -308 polymorphism does not influence the production of TNF-α. The serum level of TNF-α was positively correlated with HCV infection. Taken together, these findings suggest that the TNF-α -308 polymorphism may not be a host genetic factor associated with the severity of HCV infection, but may be an independent risk factor for HCC. | Roba M.Talaat Ahmed A.Esmail Reda Elwakil Adel A.Gurgis Mahmoud I.Nasr | 2012 | Chinese Journal of Cancer2012，31，1： | 9 |
| 2 | Cytokine and apoptosis gene polymorphisms influence the outcome of hepatitis C virus infection显示文摘BACKGROUND:Hepatitis C virus (HCV) infection is thought to be chronic and the factors leading to viral clearance or persistence are poorly understood.This study was undertaken to investigate the possibility of a significant relationship between the spontaneous clearance or the persistence of hepatitis C virus (HCV) infection and cytokine and apoptosis gene polymorphisms in Tunisian patients on hemodialysis.METHODS:Polymorphisms of the genes IL-1 (-889 IL-1α,-511 and +3954 IL-1β,IL-1Ra),IL-18 (-137 and-607),IL-12 (-1188) and Apo1/Fas (-670) were determined by PCR-RFLP,PCR-SSP and PCR-VNTR in 100 healthy blood donors and 100 patients infected with HCV and undergoing hemodialysis.The patients were classified into two groups:G1 consisted of 76 active chronic hepatitis patients (positive for HCV RNA) and G2 consisted of 24 hemodialysed patients who spontaneously eliminated the virus (negative for HCV RNA).RESULTS:The frequency of genotype association [-137GC/-607CA] IL-18 was higher in G2 (41.7%) than in G1 (15.8%) (P=0.008;OR=0.26;95% CI,0.10-0.73).We also found a higher frequency of the AA genotype of the Apo1/Fas gene in G2 (41.6%) than in G1 (17.5%) (P=0.026;OR=3.49;95% CI,1.13-10.69).Adjustment for known covariate factors (age,gender and genotype) confirmed these univariate findings and revealed that the genotype association GC-CA of the (-137 and-607) IL-18 gene and the AA genotype of the Apo1/Fas gene were associated with the clearance of HCV (P=0.041 and 0.017,respectively).CONCLUSION:The two genotypes GC-CA of the (-137 and-607) IL-18 polymorphism and the AA genotype of the Apo1/Fas gene influence the outcome of HCV infection in Tunisian patients on hemodialysis. | Leila Ksiaa Cheikhrouhou Imen Sfar Hajer Aounallah-Skhiri Houda Aouadi Salwa Jendoubi-Ayed Taieb Ben Abdallah Khaled Ayed Yousr Lakhoua-Gorgi | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 4 |
| 3 | Hepatitis-related hepatocellular carcinoma: Insights into cytokine gene polymorphisms显示文摘Hepatocellular carcinoma(HCC) is a primary liver cancer, which is one of the most prevalent cancers among humans. Many factors are involved in the liver carcinogenesis as lifestyle and environmental factors. Hepatitis virus infections are now recognized as the chief etiology of HCC; however, the precise mechanism is still enigmatic till now. The inflammation triggered by the cytokine-mediated immune response, was reported to be the closest factor of HCC development. Cytokines are immunoregulatory proteins produced by immune cells, functioning as orchestrators of the immune response. Genes of cytokines and their receptors are known to be polymorphic, which give rise to variations in their genes. These variations have a great impact on the expression levels of the secreted cytokines. Therefore, cytokine gene polymorphisms are involved in the molecular mechanisms of several diseases. This piece of work aims to shed much light on the role of cytokine gene polymorphisms as genetic host factor in hepatitis related HCC. | Mahmoud Fathy Dondeti Eman Anwar El-Maadawy Roba Mohamed Talaat | 2016 | World Journal of Gastroenterology2016，22，30： | 4 |
| 4 | 慢性丙型肝炎患者血清IL-18和IL-1β水平的变化及意义显示文摘目的:探讨血清IL-18和IL-1β水平在慢性丙型肝炎患者病程进展中的作用及与临床和干扰素疗效的关系.方法:检测30例慢性丙型肝炎患者干扰素治疗前后血清IL-18和IL-1β含量,观察HCV感染后不同阶段血清IL-18和IL-1β水平的变化,分析血清IL-18和IL-1β水平与ALT水平、HCV基因型及Th1/Th2相关细胞因子IL-2和IL-6的关系.比较应答组和无应答组之间2种细胞因子水平的差异.血清细胞因子检测应用ELISA法,HCV基因分型应用直接测序法,HCV RNA载量采用荧光定量PCR法.结果:慢性丙型肝炎患者血清IL-18水平明显高于健康对照组(1077.44±657.58ng/Lvs259.92±328.47ng/L,P<0.001),IL-1β水平与健康对照组无显著差异;随着感染时间的延长,血清IL-1β水平有逐渐升高趋势,而IL-18则逐渐下降;慢性肝炎重度患者血清IL-1β水平明显高于轻度患者(4.99±1.44ng/Lvs3.68±0.76ng/L,P<0.05);不同基因型和亚型之间两种细胞因子水平无显著差异;血清IL-18与IL-2含量呈显著性正相关(r=0.434,P<0.05),与IL-6无相关,血清IL-1β含量与IL-2和IL-6均无相关;干扰素对应答组和无应答组治疗前、后血清IL-18和IL-1β水平均无显著性差异.结论:血清IL-18和IL-1β水平分别与丙型肝炎的慢性化和病情轻重相关,该两种细胞因子水平与干扰素疗效无关,不能对疗效进行预测. | 张琳 苗亮 付海超 赵桂珍 冯国和 窦晓光 | 2009 | 世界华人消化杂志2009，17，20： | 4 |
| 5 | 骨髓间充质干细胞治疗肝纤维化的研究进展显示文摘骨髓间充质干细胞是一种多能干细胞,具有分化成肝细胞的潜力.近年研究表明,骨髓间充质干细胞还具有很强的抗肝纤维化的作用,并且不发生同种异体移植排斥反应,这为肝纤维化、肝硬化的治疗带来了新的策略. | 胡国华 沈来根 | 2007 | 世界华人消化杂志2007，15，34： | 2 |
| 6 | 云南汉族人群IL-10基因启动子多态性与HCV慢性感染的相关性研究显示文摘目的探讨IL-10基因启动子SNP-1082G/A(rs1800896)、-819C/T(rs1800871)、-592C/A(rs1800872)位点与HCV慢性感染的相关性.方法入选云南地区汉族人群HCV慢性感染患者379例,健康对照364例.采用Taq Man探针基因分型方法对IL-10基因启动子SNP-1082G/A(rs1800896)、-819C/T(rs1800871)、-592C/A(rs1800872)的3个位点进行基因分型,并构建单倍型,评估上述3个SNPs及单倍型与HCV慢性感染的相关性.结果在病例组和对照组中,IL-10基因启动子SNP-1082G/A(rs1800896)、-819C/T(rs1800871)、-592C/A(rs1800872)基因型频率和等位基因频率差异没有统计学意义(P>0.05).单倍型分析结果显示,SNP-1082G/A(rs1800896)、-819C/T(rs1800871)和-592C/A(rs1800872)构建的单倍型频率的差异无统计学意义(P>0.05).结论在云南汉族群体中,IL-10基因启动子SNP-1082G/A(rs1800896)、-819C/T(rs1800871)、-592C/A(rs1800872)与HCV慢性感染没有相关性. | 李莹 张淑琼 俞建昆 汪亚玲 姚宇峰 史荔 | 2015 | 昆明医科大学学报2015，36，1： | 1 |
| 7 | 病毒性肝炎后肝纤维化的遗传易患性研究进展显示文摘肝纤维化主要由慢性肝脏损伤所致,其中部分患者可进展为肝硬化、肝衰竭或肝癌。肝纤维化的进展表现为明显的个体差异,即使将感染年龄、性别、外界因素等调整后,这种差异仍然存在。因此,宿主的遗传因素在疾病的进展中起重要作用。肝纤维化是受多基因控制的复杂过程。肝纤维化遗传的关联研究分为单核苷酸标记、单体型分析、全基因组相关研究等,这些研究结果为肝纤维化的病因及个性化治疗提供了依据。然而,这些相关研究的结果却不尽相同,主要是由于研究样本小、设计不合理等原因导致。这需要多中心合作,用良好分型的大样本来增加结果的可靠性和稳定性。 | 何雪娟 田庆宝 | 2012 | 医学综述2012，18，18： | 0 |
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