|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Meta-analysis of the relationship between Epstein-Barr virus infection and clinicopathological features of patients with gastric carcinoma显示文摘Epstein-Barr virus (EBV) infection has been causally associated with occurrence of many malignant neoplasms. EBV-encoded small RNAs (EBERs) have been detected from about 10% of gastric carcinoma tissue cells, suggesting that EBV infection is associated with the development of gastric carcinoma. The present study pooled the data from the papers concerning EBV-related gastric cancers and performed a meta-analysis of 22 research papers. Among these papers, a total of 5475 cases with gastric cancer were enrolled, of whom 411 cases were found EBV-positive, with the EBV-positive rate being 7.5%. Among the EBV-positive gastric cancer cases, the detection rate was 11.1% in males and 3.0% in females. Compared with EBV-negative gastric cancer, EBV-positive gastric cancer had less lymph node metastasis. Based on the histological typing, of the EBV-positive gastric cancers, the diffuse type was 8.1%, and intestinal type was 8.0%. The examined specimen types included stored paraffin blocks and fresh surgically removed specimens, their EBV positive rates were 7.9% and 6.5% respectively. In terms of geographical distribution, the detection rate of EBV-positive gastric cancer was 9.4% in America, 6.1% in Asia and 9.1% in Europe. Meta-analysis showed that EBV infection occurred only in gastric cancer tissue cells and was significantly associated with the patients’ gender, lymph node metastases, and the location where tumor tissue generated and geographical distribution (P<0.05), but was not significantly associated with the patients’ histological types of tumor and the types of specimens (P>0.05). These results suggested that EBV-positive gastric cancer has distinct clinicopathological features. | LI ShuYing1,2, DU HaiJun3, WANG Zhan3, ZHOU Ling3, ZHAO XiaoYu1 & ZENG Yi3 1 College of Life Sciences, Hebei University, Baoding 071002, China 2 Department of Pathogenic Biology, Faculty of Biological Science, North China Coal Medical College, Tangshan 063000, China 3 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese CDC), State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Beijing 100052, China | 2010 | Science China(Life Sciences)2010，53，4： | 17 |
| 2 | 广州地区EB病毒相关胃癌的临床病理特征及相关分子表达显示文摘目的 观察鼻咽癌高发区广州地区EB病毒相关胃癌的构成比、临床病理特征、EB病毒的潜伏类型,并初步探讨DNMT1、p16和cyclin D1在发病过程中的作用.方法 对676例胃癌采用组织芯片和EBER1原位杂交的方法筛选EB病毒相关胃癌,并用免疫组织化学EnVision法检测EB病毒潜伏期膜蛋白(LMP)和DNMT1、p16和cyclin D1的表达.结果 在676例胃癌中,45例EB病毒阳性(6.7%),EB病毒相关胃癌以男性为主,主要发生在胃的中上2/3,以弥漫型多见(P<0.05).EBNA1和LMP2A的阳性例数分别为42例(93.3%)和24例(53.3%),EBNA2、LMP1和ZEBRA均未见表达.DNMT1、p16和cyclin D1在45例EB病毒相关胃癌的阳性例数分别为35例(77.8%)、10例(22.2%)和29例(64.4%),在40例EB病毒阴性胃癌的阳性例数分别为20例(50.0%)、25例(62.5%)和12例(30.0%),3个分子在两组胃癌的表达率差异均具有统计学意义(P<0.05).p16与肿瘤的浸润深度有关(P<0.05).LMP2A与DNMT1、DNMT1与p16、p16与cyclin D1存在相关关系(P<0.05).结论 广州地区EB病毒相关胃癌占胃癌构成比的6.7%(45/676),EB病毒的潜伏类型部分为Ⅰ型,部分介于Ⅰ型和Ⅱ型之间.LMP2A、DNMT1、p16和cyclin D1的相互作用在EB病毒相关胃癌发生过程中起着重要的作用. | 韩静 何丹 冯智英 丁运刚 邵春奎 | 2010 | 中华病理学杂志2010，39，12： | 11 |
| 3 | EB病毒潜伏期膜蛋白LMP2A对胃癌细胞增殖的影响显示文摘目的:构建EBV潜伏期膜蛋白基因LMP2A的腺病毒表达载体,探讨LMP2A表达对胃癌细胞的作用.方法:RT-PCR扩增获取目的基因LMP2A,采用AdEasy系统构建携带目的基因的重组腺病毒载体pAd-2A,脂质体法将重组质粒pAd-2A转染HEK293细胞包装重组腺病毒.用重组腺病毒感染靶细胞SGC(胃癌细胞),通过MTT实验、流式细胞分析、激光共聚焦检测目的基因LMP2A表达对靶细胞增殖的影响.结果:限制性酶切、PCR及测序鉴定证实,目的基因LMP2A正确插入重组质粒,HEK293细胞成功包装出具有稳定感染性的重组腺病毒,命名为vAd-2A,经测定病毒滴度为3.16×10^(12)pfu/L.MTT实验、流式细胞分析以及激光共聚焦检测结果显示,感染腺病毒vAd-2A的SGC细胞增殖旺盛(感染vAd-2A细胞vs感染vAd细胞和未感染病毒细胞,P<0.01),S期细胞比例升高(24 h:35.2%±5.1%vs 14.0%±3.4%,13.2%±4.6%,P<0.01;48 h:25.6%±4.1%vs 12.9%±2.6%,12.5%±3.2%,P<0.01),LMP2A可诱导细胞周期调节蛋白cyclinE的表达.结论:成功构建了EBV潜伏期膜蛋白基因LMP2A的重组腺病毒表达载体,并在HEK293细胞中成功包装出重组腺病毒,同时证实LMP2A可通过诱导cyclinE的表达促进SGC细胞增殖. | 高玉 纪静 朱伟 王云 罗兵 | 2007 | 世界华人消化杂志2007，15，2： | 6 |
| 4 | EB病毒感染与胃癌患者临床病理特征相关性的Meta分析显示文摘EB病毒(EPstein-Barr virus,EBV)感染与多种恶性肿瘤的发生相关.大约10%的胃癌组织细胞中可以检测到EB病毒编码的小RNA(EBERs),表明EBV感染与部分胃癌的发生相关.为研究EBV感染与胃癌临床病理特征的相关性,本研究汇总了EBV相关胃癌的研究论文,对采用原位杂交方法检测EBV的22篇论文进行了Meta分析.22篇入选的论文中收集的胃癌病例5475例,检测到EBV阳性病例411例,EBV阳性率为7.5%.在EBV阳性胃癌中,男性检出率为11.1%,女性检出率为3.0%,男性检出率相当于女性检出率3倍多;EBV阳性胃癌与阴性胃癌相比具有较少的淋巴结转移;EBV阳性胃癌与癌组织发生部位相关,并且残胃癌中EBV感染率较高.依据组织学分型,EBV阳性胃癌弥漫型为8.1%,肠型为8.0%.统计分析显示,EBV感染与组织学分型无显著相关性(P>0.05);被检标本类型包括存档蜡块和新鲜手术切除组织标本:EBV阳性率分别为7.9%,6.5%.统计分析表明,EBV相关胃癌与标本类型无显著相关性(P>0.05);在地域分布方面,EBV阳性胃癌检出率美洲为9.4%,亚洲为6.1%,欧洲为9.1%,统计分析显示,EBV相关胃癌与地域分布显著相关(P<0.05).Meta分析表明,EBV感染仅发生在胃癌组织细胞中,并且与患者性别、淋巴结转移、肿瘤组织发生部位及地域分布显著相关(P<0.05),与患者肿瘤组织学分型、标本类型无显著相关性(P>0.05).结果提示,EBV阳性胃癌具有独特的临床病理学特征. | 李淑英 杜海军 王湛 周玲 赵晓瑜 曾毅 | 2009 | 中国科学(C辑)2009，39，9： | 5 |
| 5 | 肺癌组织中EB病毒感染的检测显示文摘目的探讨原发性肺癌中EB病毒(Epstein—Barr virus,EBV)的存在情况及EBV与原发性肺癌的关系。方法唐山市人民医院和开滦医院病理科储存的2001--2006年肺癌手术切除石蜡包埋肺癌组织108份,癌旁组织22份,以EBV阳性鼻咽癌组织为阳性对照,用原位杂交法(ISH)检测肺癌患者石蜡包埋组织标本中EBV编码的小RNA(EBERl),并采用图像分析法进行形态学定量。结果癌组织及癌旁组织EBERl的阳性率分别为33.3%(36/108)和4.5%(1/22),二者间差异有统计学意义(P〈0.01)。鳞癌、腺癌、小细胞癌及大细胞癌中EBV感染率分别是35.9%(14/39)、31.6%(12/38)、31.0%(9/29)和1/2。EBV感染与患者年龄、性别和组织学类型无关,但与肺癌的部位、癌组织分化程度有关,右肺明显高于左肺,中低分化癌明显高于高中分化癌。结论唐山地区原发性肺癌组织中EBV感染率为33.3%,EBV感染可能是肺癌的潜在病因之一,在癌组织分化的不同阶段有不同的作用。 | 李翠梅 韩光亮 张淑杰 | 2007 | 中华实验和临床病毒学杂志2007，21，3： | 5 |
| 6 | EBV相关性胃癌研究进展显示文摘胃癌细胞中存在Epstein-Barr病毒(EpsteinBarr virus,EBV)者被称为EBV相关性胃癌(Epstein-Barr virus-associated gastric carcinoma,EBVaGC).近年来EBVaGC作为一种独特的分子亚型疾病逐渐被人们所认知,全球胃癌患者中平均有10%者为EBVaGC.本文对EBVaGC近年来在流行病学、临床病理特征、发病机制、治疗及预后等方面的研究进展作一综述.但目前对EBVaGC的研究尚不明确,且尚无临床诊疗规范与共识。 | 朱晓芸 李夏 李素琴 于红刚 | 2017 | 世界华人消化杂志2017，25，15： | 2 |
| 7 | EB病毒与相关性胃癌研究进展显示文摘EB病毒(Epstein-Barr virus,EBV)于40年前从1个伯基特淋巴瘤活组织切片中发现,是发现的第1个与人类癌症直接相关的病毒.他是一个常见的人类病原体,与宿主保持平衡的状态,在极少数情况下,宿主一病毒之间的平衡被打乱.从而造成不同群体的恶性肿瘤.EBV与上皮细胞肿瘤特别是鼻咽癌和EBV阳性胃癌相关性还不是太清楚,目前认为是病毒在上皮细胞中显示癌前病变病变的基因变化的后果.约10%的胃癌中有克隆的EBV.本文对EBV在胃癌发生发展中的作用进行了讨论,包括流行病学和临床研究. | 李晓 孙自勤 江学良 | 2011 | 世界华人消化杂志2011，19，32： | 2 |
| 8 | 嗅神经母细胞瘤与EB病毒感染的关系显示文摘目的探讨Epstein-Barr病毒(EBV)在嗅神经母细胞瘤中的表达。方法应用免疫组织化学方法(Envision法)检测11例嗅神经母细胞瘤EBV抗原的蛋白表达。结果嗅神经母细胞瘤EBV感染阳性率为45.5%(6/11),嗅神经母细胞瘤检出率明显高于鼻黏膜炎症者(P<0.05);嗅神经母细胞瘤中的肿瘤细胞含有EBV潜伏感染期中高拷贝(EBV)含量丰富,阳性信号在强胞质、细胞膜表达。结论嗅神经母细胞瘤存在EBV的感染;嗅神经母细胞瘤发生可能与EBV潜伏人鼻黏膜感染有关。 | 叶圣华 陈余鹏 王泽鸿 | 2006 | 福建医科大学学报2006，40，6： | 2 |
| 9 | EB病毒相关胃癌的研究新进展显示文摘胃癌是消化道最常见的恶性肿瘤之一,其发病率在世界范围内居各类恶性肿瘤第2位,在国内居各类恶性肿瘤之首,严重危害着人民的健康。胃癌的发生发展是一个复杂的、多因素、多步骤的过程,其发病机制及病因一直是国内外学者的研究热点。EB病毒是一种γ-DNA疱疹病毒,在世界范围内的人群中,其感染率达95%左右。 | 文亦磊 张锡流 | 2015 | 广东医学2015，36，8： | 2 |
| 10 | EBV相关胃癌发生发展的研究进展显示文摘 | 彭璇 严米娅 | 2009 | 湖北民族学院学报(医学版)2009，26，1： | 1 |
| 11 | EB病毒与胃癌发生的研究进展显示文摘全球恶性肿瘤发病率中胃癌居第2位,近年来的研究不断证实Epstein-Barr(EB)病毒存在于胃癌细胞中,EB病毒相关胃癌(Epstein-Barr virus-associated gastric carcinoma,EBVaGC)逐渐被人们所了解和认知.全球胃癌患者中平均有7.5%是EBVaGC,预计每年有80000新增EBVaGC患者.EBVaGC具有自己独特的临床病理学特性,EB病毒编码的小RNA在肿瘤细胞中表达而不在正常上皮细胞中表达,说明EB病毒在胃癌的发生中起着重要的作用.本文对EB病毒及EBVaGC做一综述. | 郭欢 王涛 苏海翔 | 2013 | 世界华人消化杂志2013，21，17： | 1 |
| 12 | EB病毒与胃癌关系的研究进展显示文摘 | 刘国莉 唐世孝 | 2010 | 西南军医2010，12，5： | 0 |
| 13 | Gastric cancer research in Mexico:A public health priority显示文摘This study aimed review studies conducted on Mexican patients diagnosed with gastric cancer and/or diseases associated with its development,in which at least one Mexican institute has participated,and to assess their contributions to the primary and secondary prevention of this disease.A search of the Medline database was conducted using the following keywords:gastric/stomach cancer,Mexico.Studies of the Mexican population were selected in which at least one Mexican Institute had participated and where the findings could support public policy proposals directed towards the primary or secondary prevention of gastric cancer.Of the148 studies found in the Medline database,100 were discarded and 48 were reviewed.According to the analysis presented,these studies were classified as:epidemiology of gastric cancer(5/48);risk factors and protectors relating to gastric cancer(9/48);relationship between Helicobacter pylori and pathologies associated with gastric cancer and the development of the disease(16/48);relationship between the Epstein-Barr virus and pathologies associated with gastric cancer and the development of the disease(3/48);molecular markers for the development of diseases associated with gastric cancer and gastric cancer(15/48).Mexico requires a program for the prevention and control of gastric cancer based on national health indicators.This should be produced by a multidisciplinary committee of experts who can propose actions that are relevant in the current national context.The few studies of gastric cancer conducted on the Mexican population in national institutes highlight the poor connection that currently exists between the scientific community and the health sector in terms of resolving this health issue.Public policies for health research should support projects with findings that can be translated into benefits for the population.This review serves to identify national research groups studying gastric cancer in the Mexican population. | Clara Luz Sampieri Mauricio Mora | 2014 | World Journal of Gastroenterology2014，20，16： | 0 |
| 14 | EB病毒相关胃癌研究进展显示文摘Epstien-Barr病毒(EBV)在EBV阳性胃癌的演进中有重要作用。最近的研究集中在本病的流行病学和生物学发病机制。复习近年来针对EBV相关胃癌有关的基础、临床和流行病学研究,讨论EBV在胃癌发生发展中的作用,介绍此领域最新的研究成果。 | 王长亮(综述) 崔凯(综述) 李胜(审校) | 2009 | 国际肿瘤学杂志2009，36，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员