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| 1 | 异叶苦竹花粉管生长及双受精过程(英文)显示文摘以异叶苦竹为材料,采用扫描电镜、荧光显微镜技术及传统的石蜡制片技术,解剖观察其花粉管生长途径及双受精过程。结果表明:(1)授粉后,花粉在柱头上吸水膨胀,约30min即可萌发。(2)授粉1~2h后花粉管可达到花粉长度的5~10倍,花粉管在柱头分支中进一步伸长,并开始伸入花柱中生长。(3)授粉后5h,大量花粉管沿引导组织进入花柱基部与子房顶部之间的子房壁,有少量花粉管在子房壁与外珠被之间的缝隙中生长。(4)授粉后8h,少量花粉管到达珠孔端。(5)授粉后15~18h,精核与极核融合,形成初生胚乳核;精、卵核融合,形成合子。(6)授粉后20~30h,仍可在花柱中见到大量呈束状的花粉管。(7)授粉后48h,子房内的大部分花粉管出现解体,大多数花粉死亡。研究认为,精细胞到达胚珠的时间为8h。 | 林树燕 赵荣 徐强 王曙光 丁雨龙 | 2015 | 西北植物学报2015，35，11： | 8 |
| 2 | Abnormal Structure of Embryo Sac in Autotetraploid Rice显示文摘The structures of mature embryo sacs in 13 genetic stock lines of autotetraploid rice (Oryza sativa L.), including indica, japonica and javanica, were studied by using the whole-mount stain-clearing laser scanning confocal microscopy (WCLSM). Among the 13 autotetraploid rice, the majority of ovaries possess normal polygonum-type embryo sacs, while a few ovaries were characterized by abnormal embryo sacs. The abnormalities of embryo sacs could be classified into six categories, i. e. no female germ unit, abnormal polar nuclei, embryo sac degeneration, no egg apparatus, small embryo sac and ‘double set’ of embryo sacs. The frequency of abnormal embryo sac in japonica (26.6%) was higher than that in indica (19.34%). In addition, the major abnormalities in each autotetraploid line varied, suggesting that the abnormalities may be related to the genotypes of the varieties. | Guo Hai-bin FENG Jiu-huan Lu Yong-gen LIU Xiang-dong | 2006 | Rice science2006，13，4： | 3 |
| 3 | Pollen Grain Germination and Pollen Tube Growth in Pistil of Rice显示文摘The germination of pollen grain in vitro and the growth of pollen tube in the pistil of rice were observed with a microscope. The stigma was removed at different time points after pollination to study its effect on seed setting rate. The rice pollen grain started to germinate at 2 min after pollination and the pollen tube penetrated stigma into style in 5–10 min, 30 min later the end of pollen tube reached the bottom of ovary, and only some pollen tubes arrived at embryo sac at 40 min after pollination. Meanwhile, a small amount of callose began to deposit in the pollen tubes, a great deal of callose was observed at 50 min after pollination, whereas the pollen grain began to shrink. The growing rates of pollen tube in the rice stigma, style and ovary were 1500, 5000, and 5400 μm/h, respectively. The seed setting rate was quite low when the stigma was removed at about 10–15 min after pollination, gradually increased when it removed at 20 min to 50 min after pollination, and over 60% when it removed at 50 min after pollination and finally tended to be stable. | CHEN Shi-qiang WANG Zhong LIU Man-xi XIE Zhao-wei WANG Hui-hui | 2008 | Rice science2008，15，2： | 2 |
| 4 | Observation on Double Fertilization and Early Embryonic Development in Autotetraploid Polyembryonic Rice显示文摘The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy.Some abnormalities including degenerated ovary,abortive embryo sac,single fertilization,double-ovule and double-embryo and so on.were found during double fertilization and embryo development in IR36-Shuang.The rate of the abnormalities was 46.67%in IR36-Shuang,significantly higher than that in the control,an autotetraploid rice line IR36-4X(33.00%) .Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang. | DAI Xi-mei YANG Xu HUANG Qun-ce QIN Guang-yong | 2009 | Rice science2009，16，2： | 1 |
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