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| 1 | 低氮对产油尖状栅藻荧光特性及色素蛋白复合物的影响显示文摘以产油绿藻尖状栅藻(Scenedesmus acuminatus)为实验材料,采用77 K低温荧光、SDS-PAGE圆盘电泳、i TRAQ蛋白定量等方法,研究其在低氮胁迫产油条件下,藻细胞的低温荧光特性及光合色素蛋白复合物的变化。结果表明,在77 K低温条件下尖状栅藻有3个荧光发射峰,分别位于685、695和715 nm处。利用圆盘电泳从藻细胞类囊体膜上分离到6个条带,从上至下依次为CPIa、CPI、CPa1、CPa2、LHCP和FP(游离色素),与对照组(18 mmol·L-1 Na NO3)相比,低氮(3.6 mmol·L-1Na NO3)培养的藻细胞光能耗散增强,分离的类囊体膜色素蛋白复合物代表PSII的条带CPa1和C Pa2模糊,D1蛋白及捕光色素蛋白表达下调。综上低氮限制条件下产油尖状栅藻的PSII核心复合物降解,制约藻细胞吸收和转化光能,进而影响光合效率。 | 罗舒怀 张莹 吴琼芳 彭倩倩 李爱芬 张成武 | 2015 | 植物生理学报2015，51，9： | 3 |
| 2 | 聚球藻7942光自养培养的碳代谢和能量代谢显示文摘代谢通量分析是研究微藻光自养培养过程中CO2和光能利用的一个非常有用的工具。本研究建立了聚球藻7942光自养培养代谢网络,并通过代谢通量方法分析了不同入射光强下的碳代谢流分布和能量代谢。研究结果表明,CO2固定是代谢能量和还原力消耗的主要途径,分别约占总消耗能量的85%和70%。研究还发现在一定光强范围,基于ATP生成的细胞得率和最大细胞得率基本维持不变,分别约为2.80g/molATP和2.95g/molATP,但基于总吸收光能的细胞得率和对应的光能转换效率则随着光强的增加而降低。 | 颜日明 张志斌 朱笃 储炬 | 2009 | 生物工程学报2009，25，9： | 2 |
| 3 | Sunlight multiband photon flux density detection based on PFD calculation model显示文摘The sunlight provides essential light and temperature for photosynthesis in protected cultivation.Sunlight is one of the important plant living environment factors in facility agriculture.Most of the existing light detecting equipment are used to detect the whole band of Photosynthetic Available Radiatio(PAR),which is unable to meet the demand of modern photosynthesis research.In order to solve the problem,a function model between single-band spectral Photon Flux Density(PFD)and solar altitude was established through experiments.Based on the model,a sunlight multiband PFD detecting device was designed,which was using a PAR sensor as the detecting node and microcontroller as the core part.This device can detect the PFD of different bands in PAR by using a single sensor.Meanwhile,detecting band can be set by using keyboard according to the characteristic of spectrum absorption of different plants.The secure digital memory card(SD card)was used in the device to store data.Results of the field test showed that determination coefficients of the device testing red and blue PFD with standard value were 0.986 and 0.993 respectively.The device with little relative error and high reliability could be applied in facility light detecting. | Liang Yan Zhang Haihui Hu Jin Zhai Changyuan | 2015 | International Journal of Agricultural and Biological Engineering2015，8，2： | 0 |
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