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| 1 | Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions显示文摘AIM: To study the expression of early growth response gene1 (Egr-1 gene) and Bcl-X/L protein and its relationship with the cell apoptosis in human esophageal carcinoma(EC) and precancerous lesions.METHODS: In situ hybridization(ISH), immunohistochemistry (IHC) and TUNEL method were used respectively to detect Egr-1mRNA, Egr-1 protein, apoptosis related-protein Bcl-X/L and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa.RESULTS: Egr-1 gene in situ hybridization, Bcl-X/L immunohistochemistry positive products were located in the cytoplasm, while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei. The apoptosis index(AI) and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P<0.01) and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens (P<0.01).The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues (P<0.01), while the AI of Bcl-X/L positive cancer tissues was much lower than that of Bcl-X/L negative cancer tissues (P<0.01). The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC.CONCLUSION: Cell apoptosis was present through esophageal carcinogenesis. The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus. The AI was increased significantly in Egr-1 positive squamous cell carcinoma. Egr-1 might promote apoptotic effect. Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis. | Ming-Yao Wu Ying-Rui Liang Xian-Ying Wu,Department of Pathology,Shantou university Medical College,Shantou 515031,Guangdong Province,China Chu-Xiang Zhuang,Department of physiology,Shantou university Medical College,Shantou 515031,Guangdong Province,China | 2002 | World Journal of Gastroenterology2002，8，6： | 25 |
| 2 | Experimental and clinic-opathologic study on the relationship between transcription factor Egr-1 and esophageal carcinoma显示文摘AIM To observe the growth suppression effect of exogenous introduction of early growth response gene-1 (Egr-1 gene) on esophageal carcinoma tissue as well as on esophageal carcinoma cell line Eca109 and to explore the potential application of Egr-1 gene in gene therapy of tumor.METHODS Eukaryotic expression vector of PCMV-Egr-1 plasmid was introduced into Eca109 cell line which expressed no Egr-1 protein originally with lipofectamine transfection method. The introduction and expression of PCMV-Egr-1 plasmid into Eca109 cell line was confirmed by G418 selection culture, PCR amplification of neogene contained in the vector, Western blot analysis and immunocytochemical analysis. The cell growth curve,soft agar colony formation rate and tumorigenicity in SClD mice were examined to demonstrate the growth suppression effect of exogenous Egr-1 gene on Eca109 cell line. The Egr-1 mRNA and Egr-1 protein were also detected in 50 surgical specimens of esophageal carcinoma by in situ hybridization and immunohistochemistry.RESULTS Exogenous Egr-1 gene was introduced successfully into Eca109 cell line and expressed Egr-1 protein stably. The transfected Eca109 cell line grew more slowly than control Eca109 as shown by cell growth curves, the soft agar colony formation rate (4.0% vs 6.9%, P<0.01) and the average growth rate of tumor in SCID mice (35.5 ± 7.6 vs 65.8 ± 7.6, P<0.05). The expression level of Egr-1 mRNA and protein significantly increased in dysplastic epithelia adjacent to cancer rather than in cancer tissues (65.8% vs 20.0% by ISH and 57.9% vs 14.0% by IHC, P<0.01).CONCLUSION Exogenous Egr-1 gene shows the strong effect of growth inhibition in Eca109 cell line. Egr-1 in the cancer tissue shows down-regulated expression that supports the inhibited function of Egr-1 in cancer growth and suggests Egr-1 may have an important role in gene therapy of esophageal carcinoma. | Ming-Yao Wu Mao-Huai Chen Ying-Rui Liang Guo-Zhao Meng Huan-Xing Yang Chu-Xiang Zhuang Department of Pathology,Shantou University Medical College,Shantou 515031,Guangdong Province,China Supported by the National Natural Science Foundation of China,No.39670298. | 2001 | World Journal of Gastroenterology2001，7，4： | 20 |
| 3 | 慢性乙型肝炎肝组织中HLA-DR和HSP70的表达意义显示文摘目的探讨 HLA-DR 抗原和热休克蛋白70(HSP70)在慢性乙型肝炎肝组织中的表达及其意义.方法采用免疫组化技术对36例慢性乙型肝炎和20例正常肝组织中 HLA-DR 抗原和 HSP70的表达进行检测.结果正常肝组织中肝细胞未见 HLA-DR 表达.慢性乙型肝炎肝细胞 HLA-DR 表达阳性率为28%,其中,中重度肝炎HLA-DR 阳性率明显高于轻度肝炎(阳性率分别为38%和20%,x^2=13.6,P<0.01).慢性乙型肝炎和正常肝组织中肝细胞 HSP70表达阳性率分别为45%和15%,两者相比差异显著(x^2=6.3,P<0.05).中度和重度肝炎 HSP70阳性率明显高于轻度肝炎(阳性率分别为63%和30%,x^2=3.9,P<0.05).结论肝细胞 HLA-DR 和 HSP70的异常表达在慢性乙型肝炎免疫损伤和免疫保护中起重要作用。 | 蒋业贵 王宇明 李奇芬 | 2001 | 世界华人消化杂志2001，9，8： | 8 |
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