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| 1 | The Distribution of DEN Infected People in Dushan and Xingyi Area of Yunnan-Guizhou Plateau,China显示文摘The dengue viruses (DEN, genus Flavivirus, family Flaviviridae) are mosquito borne and have caused 100 million cases of dengue fever each year in most tropical and subtropical areas of the world. However, in the Southwest area of Yunnan-Guizhou Plateau, China, the previous work demonstrated that different geographic strains of Aedes albopictus were susceptible to dengue virus. In this study, we collected 456 sera samples from patients with fever and 994 sera samples from healthy population in Dushan and Xingyi area of Yunnan-Guizhou Plateau, China. All sera samples were tested for dengue IgG by enzyme-linked immunosorbent assay (ELISA). Patients’ sera samples were tested for dengue IgM and DEN antigen was checked in the sera of 6 from 456 samples with which C6/36 cell in culated by IFA. The results indicate that these patients with fever were infected with DEN-2 and suggest that DEN infection had existed in Dushan and Xingyi area of Yunnan-Guizhou Plateau, China. | Wei Liu Li ZUO Yongbing Zhou | 2006 | Cellular & Molecular Immunology2006，3，6： | 1 |
| 2 | 贵州独山、兴义不明原因发热病人登革病毒的分离和鉴定的初步研究显示文摘目的对贵州独山、兴义两地夏秋季不明原因发热病人血清进行登革病毒(DEN)分离及鉴定,从病原学角度证实贵州省人群DEN的感染情况。方法于2005年6至10月份收集贵州独山县、兴义市两地不明原因发热病人血清356份,并接种于生长良好的单层C6/36细胞盲传3代,观察细胞病变,用抗DEN1~4型单克隆抗体通过间接免疫荧光法进行型别鉴定;用DENNS1基因区特异性通用引物进行RT-PCR检测分离的DEN毒株核酸,经序列测定并作系统发生树分析。结果3份病人血清标本可使C6/36发生细胞病变,用单克隆抗体、RT-PCR扩增和序列测定,鉴定为DEN2;系统发生树分析证明,分离的病毒与DEN2-43、DEN2-44株系统进化关系最近。结论贵州省独山、兴义两地人群中存在DEN感染。 | 周永兵 左丽 刘伟 谢庭华 何成友 王定明 | 2008 | 中华微生物学和免疫学杂志2008，28，3： | 0 |
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