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| 1 | Mitochondrial DNA4977-bp deletion correlated with reactive oxygen species production and manganese superoxide dismutase expression in gastric tumor cells显示文摘背景 Mitochondrial DNA 4977-bp 删除(mtDNA4977 ) 在许多人的瘤形成被报导。然而,它的生物意义尚待被评估,分子的机制需要被调查。在这研究,我们在胃的癌症( GC )分析了 mtDNA4977 的频率房间线和纸巾,象反应的氧一样,种( ROS )内容和锰 superoxide dismutase ( MnSOD )表示在 GC 细胞线铺平探索它的生物意义和分子的 mechanism.Methods 半量的 PCR 和即时 PCR 被用来在 13 根 GC 细胞线和 272 人的胃的纸巾检测 mtDNA4977 的发生( 108 GC 标本和各自的邻近的正常纸巾,和 56 标准我们进一步由半量的反向的 transcription-PCR (RT-PCR ) 并且西方的弄污由流动 cytometry 和 MnSOD 表示识别了细胞内部的 ROS 生产。统计分析用基于我们的更早的学习的逻辑回归分析和 Kaplan-Meier method.Results 被执行,我们由减少周期数字优化了 PCR 扩大条件。在这研究,我们系统地在 GC 房间线记录了 mtDNA4977 的高发生(10/13, 76.9%) , GC 纸巾(86/108, 79.6%) ,匹配的正常纸巾(73/108, 67.6%) ,并且非癌症病人的正常胃的 mucosa (29/56, 51.8%) 。变异的 mtDNA4977 的显著地更高的发生关于邻近的正常纸巾在 GC 纸巾被观察(79.6% 对 67.6% , P=0.045 ) ,并且他们在正常控制比那高是两个(P < 0.05 ) 。最重要地,我们与 MnSOD 和 ROS 内容的表示水平连接了 mtDNA4977 变化。包含 MnSOD 的更低的表示水平的房间线被发现有通常更高的经常的 mtDNA4977 和更多的 ROS.Conclusion 减少的抗氧化的能力,导致增加的 ROS 内容,在胃的 carcinogenesis 期间与 mtDNA 损坏被相关。 | WANG Juan LV You-yong | 2009 | Chinese Medical Journal2009，，4： | 8 |
| 2 | Characterization of glucose-6-phosphate dehydrogenase deficiency and identification of a novel haplotype 487G>A/IVS5-612(G>C) in the Achang population of southwestern China显示文摘The prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency and its gene mutations were studied in the Achang population from Lianghe County in Southwestern China. We found that 7.31% (19 of 260) males and 4.35% (10 of 230) females had G6PD deficiency. The molecular analysis of G6PD gene exons 2―13 was performed by a PCR-DHPLC-Sequencing or PCR-Sequencing. Sixteen inde-pendent subjects with G6PD Mahidol (487G>A) and the new polymorphism IVS5-612 (G>C), which combined into a novel haplotype, were identified accounting for 84.2% (16/19). And 100% Achang G6PD Mahidol were linked to the IVS5-612 C. The percentage of G6PD Mahidol in the Achang group is close to that in the Myanmar population (91.3% 73/80), which implies that there are some gene flows between Achang and Myanmar populations. Interestingly, G6PD Canton (1376G>T) and G6PD Kaiping (1388G>A), which were the most common G6PD variants from other ethnic groups in China, were not found in this Achang group, suggesting that there are different G6PD mutation profiles in the Achang group and other ethnic groups in China. Our findings appear to be the first documented report on the G6PD genetics of the AChang people, which will provide important clues to the Achang ethnic group origin and will help prevention and treatment of malaria in this area. | YANG YinFeng, ZHU YueChun, LI DanYi, LI ZhiGang, Lü HuiRu, WU Jing, TANG Jing & TONG ShuFen Department of Biochemistry, Faculty of Basic Medicine, Kunming University of Medical Sciences, Kunming 650031, China These authors contributed equally to this work | 2007 | Science China(Life Sciences)2007，50，4： | 6 |
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