共被期刊论文引用了3次
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| 1 | 质谱法测定4种真菌毒素与载体蛋白偶联物的结合比显示文摘采用基质辅助激光解吸-电离飞行时间质谱法(matrix-assisted laser desorption/ionization time-of-flight massspectrometry,MALDI-TOFMS)对由3种真菌毒素伏马菌素B1(FB1)、赭曲霉毒素A(OTA)、脱氧雪腐镰刀菌烯醇(DON)与两种载体蛋白卵清白蛋白(OVA)、血清白蛋白(BSA)分别偶联制备得到的4种小分子-载体蛋白偶联物FB1-OVA、FB1-BSA、OTA-BSA、DON-OVA的偶联结合比进行测定,并与紫外吸收光谱法进行比较。基质辅助激光解吸-电离飞行时间质谱法测得偶联物FB1-OVA、FB1-BSA、OTA-BSA、DON-OVA的结合比分别为5:1、11:1、3:1、0;紫外吸收光谱法测得OTA-BSA的结合比为4.4:1,DON-OVA的结合比为3.4:1,无法测定FB1-OVA、FB1-BSA结合比。 | 王莹 王希春 金福源 何成华 郑浩 张海彬 | 2012 | 食品科学2012，33，2： | 6 |
| 2 | 免疫脂质体作为靶向给药系统的研究进展显示文摘免疫脂质体是用抗体或其片段修饰的脂质体,能与靶细胞表面抗原或受体结合,从而对靶细胞具有分子水平上的识别能力。与游离药物、非特异抗体脂质体、单独单抗等相比,免疫脂质体有更好的选择性和更强的杀伤活性。在动物体内,免疫脂质体可使药物特异性分布在病灶部位,从而增强药物疗效、减轻不良反应,并且表面聚乙二醇化还增强了体内的循环时间。本文综述了用于修饰的不同种类抗体、抗体与免疫脂质体偶联方式,并总结了免疫脂质体在抗肿瘤药、基因治疗、活体成像技术以及在传染病、自身免疫和神经退行性疾病治疗方面的应用。 | 邱明星 白姣姣 周小菊 | 2020 | 中国医药工业杂志2020，51，3： | 3 |
| 3 | Characterization and Comparison of Ochratoxin A-Ovalbumin(OTA-OVA) Conjugation by Three Methods显示文摘In order to generate an antibody against a small hapten molecule, the hapten is cross-linked with carrier protein to make it immunogenic. In this study, the hapten(ochratoxin A, OTA) was coupled to ovalbumin(OVA) by an active ester reaction. To develop a technique for detecting the conjugation, the hapten-protein conjugate(OTA-OVA) was characterized thoroughly by immunoarray technology, ultraviolet(UV) spectroscopy and matrix-assisted laser desorption ionization time-of-fl ight mass spectrometry(MALDI-TOF-MS), respectively. The molecular weight of OTA-OVA was 50 350.141 Da, and the molecular weight of OVA was 44 887.506 Da, which were determined by MALDI-TOF-MS, respectively. In OTA-OVA, the molecular coupling ratio was 13:1 by MALDI-TOF-MS while the molecular coupling ratio was 10:1 by UV. In this experiment, UV and MALDI-TOF-MS were selected as the effi cient methods to evaluate the cross-linking effect and calculate the molecular coupling ratio. | WANG Xi-chun BAO Ming WU Jin-jie LUO Ying MA Liang-you WANG Ying ZHANG Ai-hua HE Cheng-hua ZHANG Hai-bin | 2014 | Journal of Integrative Agriculture2014，13，5： | 1 |
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