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| 1 | 抗gp210及sp100抗体在原发性胆汁性肝硬化患者中的临床研究显示文摘目的探讨抗gp210、sp100抗体检测在原发性胆汁性肝硬化(PBC)患者的相关性及临床意义。方法 53例原发性胆汁性肝硬化及168例非原发性胆汁性肝硬化(非PBC)患者及50例健康对照采用免疫印迹法检测抗gp210和sp100自身抗体并观察其临床评价指标,对其结果进行回顾性分析。结果 (1)53例PBC患者采用免疫印迹法检测gp210和sp100等自身抗体结果显示其阳性率分别为41.5%和17.0%;与非PBC和对照组比较,P<0.01有非常显著性意义。(2)53例PBC患者检测gp210和sp100等自身抗体与非PBC及对照组比较,其临床评价指标结果显示敏感性为41.5%和17.0%,特异性为97.2%和99.5%。结论 PBC患者检测抗gp210和抗sp100抗体,对PBC的诊断及治疗具有重要的作用,有助于提高PBC诊疗水平。 | 詹克勤 彭卫华 陈建华 李俊明 鞠北华 胡意 罗忠勤 | 2013 | 实验与检验医学2013，31，5： | 8 |
| 2 | 23例原发性胆汁性肝硬化患者临床特征分析显示文摘目的分析原发性胆汁性肝硬化(PBC)患者临床资料,总结其临床特征,以提高对该病的诊断水平。方法回顾性分析23例PBC患者的临床资料,观察预后。结果 23例患者确诊时平均年龄为53.5±12.7岁;17例为女性;ALP均明显升高(380.6±375.3U/L),20例GGT升高(360.8±633.2U/L),15例血清总胆红素升高(72.8±97.2μmol/L),血清IgM、IgG、IgA浓度超过正常值的比率分别为78.3%、69.3%和17.4%;19例(82.6%)血清AMA及其M2亚型阳性,14例(60.9%)ANA阳性,10例(43.5%)抗gp210抗体阳性;影像学检查均未发现胆管阻塞或扩张。结论 PBC好发于中年女性,血清ALP、GGT、IgM明显升高、AMA及其M2亚型阳性有助于诊断。抗gp210抗体阳性者可能预后较差。 | 查翔远 翟群超 宋有良 胡晓春 黄义泽 吴同生 史卫红 | 2012 | 实用肝脏病杂志2012，15，2： | 7 |
| 3 | 关于抗核抗体概念及核膜型临床意义的建议显示文摘间接免疫荧光法(indirect immunofluorescence, IIF)通过改进底物选择及制备方法检测抗核抗体(antinuclear antibodies,ANA),已形成以人上皮细胞株-2(human epithelial cells-2,HEp-2细胞)为基质的标准测定方法。 | 程艳杰 王旭 朱鸿 曹华军 徐裕海 范存琳 李士军 | 2012 | 中华检验医学杂志2012，35，5： | 5 |
| 4 | 原发性胆汁性肝硬化发生失代偿的危险因素研究显示文摘目的探讨原发性胆汁性肝硬化(PBC)患者肝硬化失代偿期的危险因素指标,为临床诊治提供循证医学依据。方法回顾性分析115例PBC患者,将患者分为代偿期组(72例)和失代偿期组(43例),分析生化指标[白蛋白(Alb)、球蛋白(Glb)、总胆红素(TB)、碱性磷酸酶(ALP)、γ-谷氨酰基转移酶(GGT)、总胆汁酸(TBA)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)]、凝血指标[凝血酶原时间(PT)]、免疫指标(Ig M、Ig G、Ig A、γ-Glb)、自身抗体[抗核点型靶抗原蛋白100(sp100)抗体、抗核孔复合物糖蛋白210(gp210)抗体、抗着丝点抗体]、Mayo评分与肝硬化失代偿的相关性,并通过多因素Logistic回归分析筛选出可有效独立预测PBC患者出现失代偿的危险因素。结果失代偿期组TB、TBA、PT、γ-Glb、Ig A、Ig G水平及Mayo评分均高于代偿期组,而Alb、GGT水平低于代偿期组(P<0.05)。2个组之间Glb、Alp、ALT、AST、Ig M水平及抗sp100抗体、抗gp210抗体、抗着丝点抗体阳性率差异均无统计学意义(P>0.05)。多因素非条件Logistic回归分析显示,Alb<34.5 g/L[比值比(OR)=4.458,95%可信区间(CI)1.019~19.501]、Ig A>3.09 g/L(OR=15.41,95%CI 2.868~82.786)、Mayo评分>5.04(OR=15.7,95%CI 2.653~92.907)为PBC患者肝硬化失代偿期的独立预测指标。受试者工作特征(ROC)曲线分析显示Alb、Ig A、Mayo评分的曲线下面积分别为0.881、0.700、0.860,Alb+Ig A+Mayo联合检测模型的曲线下面积为0.912。结论 Alb<34.5 g/L、Ig A>3.09 g/L、Mayo评分>5.04及Alb+Ig A+Mayo联合检测预测概率>0.45,可预测PBC患者出现肝硬化失代偿。 | 李京华 赵伟 童晶晶 张铁 曹永彤 | 2018 | 检验医学2018，33，5： | 4 |
| 5 | 自身抗体在原发性胆汁性胆管炎中的诊断及预后价值显示文摘原发性胆汁性胆管炎(PBC)是一种病因不明的自身免疫性胆汁淤积性肝病,肝穿刺活检是重要的诊断手段,但因其有创性,临床运用有限。自身抗体对于PBC有特殊诊断及预后价值,代表性抗体有抗线粒体抗体和抗核抗体,各抗体的临床意义不尽相同。综述了PBC相关自身抗体的诊断及预后意义及相关进展。 | 党富涛 唐映梅 | 2020 | 临床肝胆病杂志2020，36，5： | 3 |
| 6 | 原发性胆汁性肝硬化患者特异性自身抗体的实验研究显示文摘目的探讨原发性胆汁性肝硬化(PBC)患者自身抗体相关性及临床意义。方法采用免疫印迹法及间接免疫荧光法检测48例PBC、182例非PBC患者及50例健康对照抗线粒体抗体M2亚型(AMA-M2)、抗核包膜蛋白抗体(GP210)、抗核多点抗体(SP100)、抗核抗体(ANA)、抗双链DNA抗体(ds-DNA)、抗线粒体抗体(AMA)、抗平滑肌抗体(SMA)及抗细胞质抗体(ACYA)等自身抗体并观察其临床评估指标,对其结果进行回顾性分析。结果48例PBC患者ANA、AMA、SMA、ACYA及ds-DNA抗体阳性率分别为41.7%、93.8%、12.5%、79.2%和2.1%;ANA、AMA、SMA和ACYA与对照组比较,P均<0.01;AMA和ACYA与非PBC组比较,P均<0.01。48例PBC患者AMA-M2、GP210及SP100抗体阳性率分别为68.8%、41.7%和16.7%;与非PBC组和对照组比较,P均<0.01。结论检测AMA-M2、GP210、SP100和AMA等自身抗体对PBC的诊断、鉴别诊断、治疗及预后具有重要的作用,可提高PBC诊疗水平。 | 彭卫华 谭立明 陈建华 李俊明 鞠北华 胡意 罗忠勤 | 2012 | 山东医药2012，52，44： | 2 |
| 7 | 自身免疫性肝病相关自身抗体的实验诊断学研究进展显示文摘自身免疫性肝病(autoimmune liver disease,AILD)包括自身免疫性肝炎、原发性胆汁性胆管炎、原发性硬化性胆管炎和IgG4相关硬化性胆管炎等。AILD患者特点是血清中可检出一种或多种自身抗体阳性,自身抗体检测对于AILD诊断至关重要。本文总结了AILD相关自身抗体的实验诊断学研究进展。 | 何超男 王雅杰 | 2021 | 标记免疫分析与临床2021，28，11： | 2 |
| 8 | Presence of disease specific autoantibodies against liver sinusoidal cells in primary biliary cirrhosis显示文摘AIM: To investigate the presence of autoantibodies directed against liver sinusoidal cells in primary biliary cirrhosis(PBC).METHODS: Liver biopsies from 21 PBC patients were studied and compared with 12 liver biopsies from disease controls [3 patients with hepatitis B(HBV) virus,3 patients with hepatitis C virus(HCV), 3 patients with non-alcoholic steatohepatitis and 3 patients with acute alcoholic hepatitis(AAH)]. As healthy controls, we used tissue specimens adjacent to metastatic liver adenocarcinoma. Normal serum was taken from staff members of the unit. The determination of the cell type targetedby autoantibodies present in the patients sera was performed by indirect immunofluorescence(IIF) analysis using paraffin-embedded liver sections as a substrate.Sera from homologous or heterologous PBC patients or sera from the disease control group were used as primary antibodies. The presence of autoantibodies was identified using confocal microscopy.RESULTS: In total, 18/21(85.7%) PBC patients exhibited positive staining in the sinusoidal cells, 10/21(47.6%) in lymphocytes, 8/21(38%) in cholangiocytes and 7/21(33.3%) in hepatocytes, when homologous serum and fluorescein isothiocyanate-conjugated immunoglobulin type G(IgG) secondary antibody were used. PBC sections incubated with heterologous PBC serum showed reduced staining(20% for sinusoidal cells, 20% for lymphocytes, 20% for cholangiocytes and 13.3% for hepatocytes). When IgM immunoglobulin, instead of IgG, was used as secondary antibody,positive staining was observed in 75% of lymphocytes,62.5% of cholangiocytes, 37.5% of hepatocytes and50% of the sinusoidal cells with a much stronger staining intensity. No staining was observed when either normal or PBC sera were used as a primary antibody on liver sections from the disease control group. When PBC sera were incubated with healthy control sections,weak positive staining of cholangiocytes was observed in 3/21(14.3%) PBC serum samples. Steatohepatitis serum on PBC sections gave a positive staining of some hepatocytes and lymphocytes but no staining on viral hepatitis sections. Incubation with HBV sera stained some hepatocytes, cholangiocytes and intra-sinusoidal or portal lymphocytes of PBC, HBV and AAH patients but not HCV patients.CONCLUSION: In this study, for the first time in diseased liver tissue, we have demonstrated that a large proportion of PBC patients have disease specific autoantibodies against liver sinusoidal cells. | Ourania Sfakianaki Maria Tzardi Argyro Voumvouraki Aikaterini Afgoustaki Meri Koulentaki Elias Kouroumalis | 2013 | World Journal of Hepatology2013，5，10： | 2 |
| 9 | 原发性胆汁性肝硬化43例临床分析显示文摘目的总结原发性胆汁性肝硬化(PBC)的临床特征,提高对该疾病的认识水平。方法对43例PBC患者的临床表现、血清学检查、肝脏病理及治疗进行回顾性分析。结果 43例PBC患者中女性40例,男性3例,确诊时年龄29-80岁,临床以持续黄疸、皮肤瘙痒、乏力、肝脾肿大、腹胀、双下肢浮肿为主要表现;血清球蛋白、Y球蛋白、碱性磷酸酶(ALP)、γ谷氨酰转肽酶(γ-GT)、总胆固醇(CHO)以及IgM明显升高;绝大多数患者抗线粒体抗体(AMA)、抗线粒体M2亚型抗体(AMA-M2)为阳性,8例患者AMA、AMA-M2阴性但病理证实为PBC;多数患者经熊去氧胆酸(UDCA)辅以其他治疗后皮肤瘙痒、黄疸、乏力、纳差等症状减轻,肝功能指标改善。结论中年女性出现胆汁淤积性肝损害伴ALP、IgM升高,且AMA及AMA-M2阳性,可诊断为PBC,并非一定依据肝脏病理活检;男性患者较少见,AMA、AMA-M2阴性者也有,此类往往需要病理协助诊断。UDCA对早期PBC患者治疗有效。 | 李娟 王越 肖健存 王艳 李旭 张云丽 刘梅 郑素军 陈煜 | 2015 | 肝脏2015，20，9： | 0 |
| 10 | Primary biliary cirrhosis: From bench to bedside显示文摘Primary biliary cirrhosis(PBC) is a chronic non-suppurative destructive intrahepatic cholangitis leading to cirrhosis after a protractive non cirrhotic stage. The etiology and pathogenesis are largely unknown and autoimmne mechanisms have been implicated to explain the pathological lesions. Many epitopes and autoantigens have been reported as crucial in the pathophysiology of the disease and T and B cells abnormalities have been described, the exact pathways leading to the destruction of small intrahepatic ductules are mostly speculative. In this review we examined the various epidemiologal and geoepidemiological data as well as the complex pathogenetic aspects of this disease, focusing on recent in vivo and in vitro studies in this field. Initiation and progression of PBC is believed to be a multifactorial process with strong infuences from the patient's genetic background and by various environmental factors. The role of innate and adaptive immunity, including cytokines, chemokines, macrophages and the involvement of apoptosis and reactive oxygen species are outlined in detailed. The current pathogenetic aspects are presented and a novel pathogenetic theory unifying the accumulated clinical information with in vitro and in vivo data is formulated. A review of clinical manifestations and immunological and pathological diagnosis was presented. Treatment modalities, including the multiple mechanisms of action of ursodeoxycholate were finally discussed. | Elias Kouroumalis George Notas | 2015 | World Journal of Gastrointestinal Pharmacology and Therapeutics2015，6，3： | 0 |
| 11 | 肝病自身抗体对原发性胆汁性胆管炎的临床价值显示文摘目的探讨肝病自身抗体抗线粒体抗体(AMA)、抗线粒体抗体-M2型(AMA-M2)、抗gp210抗体、抗sp100抗体对原发性胆汁性胆管炎(PBC)的诊断价值及预后价值。方法回顾性分析2020年3月至2022年2月于福建医科大学附属泉州第一医院就诊的88例PBC患者、100例非PBC肝功能不全者和140名健康对照者的临床资料,计算AMA、AMA-M2、抗gp210抗体和抗sp100抗体诊断PBC的灵敏度、特异度、AUC曲线下面积,并进一步比较各抗体阳性组和阴性组PBC患者肝脏酶学指标丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)和γ-谷氨酰转移酶(γ-GGT)和免疫球蛋白IgG、IgM水平差异是否显著。结果AMA、AMA-M2、抗gp210抗体、抗sp100抗体诊断PBC的灵敏度分别为70.45%、77.27%、39.77%和17.05%,特异度分别为90.42%、92.08%、95.42%、96.67%,AUC曲线下面积分别为0.8044,0.8468,0.6759,0.5686。PBC患者中AMA、AMA-M2阳性组与阴性组间AST、ALT、ALP、GGT、IgG和IgM水平差异无统计学意义(P>0.05),而抗gp210抗体、抗sp100抗体阳性组ALP、GGT和IgM水平高于阴性组,差异有统计学意义(P<0.05)。抗gp210抗体在肝硬化组与非肝硬化组的阳性率分别为60.87%(14/23)和32.31%(21/65),差异有统计学意义(P<0.05)。结论AMA、AMA-M2、抗gp210抗体、抗sp100抗体对诊断PBC均具有较高的特异度,但敏感度不一,其中AMA-M2对PBC的诊断效能较好;抗gp210抗体、抗sp100抗体与PBC疾病活动度有关,对PBC具有一定的预测价值。 | 苏春燕 吴聪莲 白羽 | 2023 | 中国医药指南2023，21，10： | 0 |
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