| 1 | 持续高眼压状态下的急性闭角型青光眼的治疗时机及疗效观察显示文摘【目的】观察持续高眼压状态下的急性闭角型青光眼的治疗时机及疗效。【方法】选择2010年1月至2015年1月本院收治的121例(121眼)持续高眼压状态下的急性闭角型青光眼患者,用药物治疗不能有效降低眼压后均采用前房穿刺联合复合小梁切除术,观察患者术后眼部表现、滤过泡功能及有无并发症,比较术后7d和30d的视力、眼压。【结果】患者术后7d眼压均控制在正常值(21mmHg)以下,平均(15.6±0.4)mmHg;术后30d眼压平均(14.2±0.3)mmHg,显著低于术前,差异有统计学意义(P〈0.05);术后视力明显提高,未发生严重并发症。【结论】对药物治疗不能有效控制高眼压的急性闭角型青光眼应尽早进行手术治疗,术后患者的眼压和视功能可得到明显改善。 | 王克 邵倩 | 2016 | 医学临床研究2016，33，12： | 14 |
| 3 | 羟喜树碱诱导的人Tenon囊成纤维细胞凋亡及其机制显示文摘背景青光眼滤过手术后人Tenon囊成纤维细胞(HTFs)增生是滤过泡功能下降的主要原因,羟喜树碱是诱导肿瘤细胞及多种非肿瘤细胞凋亡的药物。目前已有羟喜树碱诱导成纤维细胞凋亡的相关报道,但其作用机制尚不完全清楚。目的探讨羟喜树碱是否能诱导HTFs凋亡并研究其作用机制。方法取离体〈6h的供体结膜囊组织,以组织块培养法原代培养HTFs,用含质量分数10%胎牛血清(FBS)的DMEM培养基继续传代培养,用波形蛋白及角蛋白对培养的细胞进行鉴定,取3~6代细胞进行实验。分别将0.Ol、0.05、0.10g/L羟喜树碱加入培养基中作用5min,未加入羟喜树碱的细胞作为对照组,采用细胞计数试剂盒-8(CCK8)法检测各组细胞的增生能力并进行比较。用0.10g/L羟喜树碱处理细胞,继续培养24h,用annexinV/PI双染流式细胞仪检测细胞凋亡率,用JC-1染色检测HTFs线粒体膜电位,采用Westernblot法检测HTFs中半胱氨酸天冬酶-3(caspase-3)、easpase-9及线粒体/细胞质中细胞色素C(cytC)蛋白的表达,对各组中的测量结果进行比较。结果0、0.01、0.05、0.10g/L羟喜树碱组的HTFs增生值(A450)分别为0.9716±0.0608、0.8035±0.0346、0.7048±0.0446和0.6265±0.0286,总体差异有统计学意义(F=26.372,P=0.002),0.01、0.05、0.10g/L羟喜树碱组HTFsA450值均低于对照组,差异均有统计学意义(P〈0.05),以0.10g/L羟喜树碱组HTFsA。,。值最低。AnnexinV/PI双染色检测发现,0.10g/L羟喜树碱组HTFs凋亡率为(18.72±1.41)%,明显高于对照组的(3.67±O.36)%,差异有统计学意义(t=-10.374,P=0.001);0.10g/L羟喜树碱组HTFs中caspase-3、caspase-9蛋白的表达强度明显强于对照组。JC-1染色发现,0.10g/L羟喜树碱处理5min后细胞质中单体JC.1的绿色荧光明显强于对照组,而线粒体中聚合态JC-1的红色荧光弱于对照组。Westernblot检测发现,0.10g/L羟喜树碱组HTFs线粒体中cytC蛋白表达灰度值为0.0124±0.0016,高于对照组的0.0216±0.0096,而0.10g/L羟喜树碱组HTFs细胞质中eytC蛋白表达灰度值为0.0605±0.0022,低于对照组的0.0301±O.0016,差异均有统计学意义(线粒体:z=4.865,P:0.014;细胞质:t=-11.177,P=0.001)。结论羟喜树碱诱导HTFs凋亡.引起线粒体的稳态破坏.激活线粒体凋亡涂径。 | 殷雪 傅煜轩 袁志兰 | 2013 | 中华实验眼科杂志2013，31，3： | 2 |
| 4 | Management of hypotony and flat anterior chamber associated with glaucoma filtration surgery显示文摘AIM: To determine the effectiveness of pharmacological and interventional treatment of hypotony and flat anterior chamber(FAC) resulting from glaucoma filtration surgery.·METHODS: We retrospectively examined the medical records of fifty-two trabeculectomy patients(52 eyes)who developed postoperative hypotony and FAC. The management and associated complications of hypotony,changing intraocular pressure(IOP) and best corrected visual acuity(BCVA) were evaluated.·RESULTS: Of the 52 patients with hypotony, 29(56%)had a grade 1 FAC, 21(40%) had a grade 2 FAC, and only 2 had a grade 3 FAC. There was no significant difference between the mean preoperative IOP and the mean IOP at three and six months after surgery. Thirteen eyes(25%) required antiglaucomatous medication three months after surgery. The mean BCVA at 6mo after surgery was significantly reduced as compared with the mean preoperative BCVA.·CONCLUSION: Hypotonia and FAC following trabeculectomy are associated with troublesome complications that require pharmacological and/or surgical treatment. Thus, close follow-up is essential for affected patients. | Yavuz Tun Mehmet Tetikoglu Necip Kara Haci Murat Sagdik Selahattin zarpaci Mustafa Nuri Elioglu | 2015 | International Journal of Ophthalmology(English edition)2015，8，5： | 0 |
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