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| 1 | Biochemical changes in phenols,flavonoids,tannins,vitamin E,β-carotene and antioxidant activity during soaking of three white sorghum varieties显示文摘Objective:To investigate the changes in total phenols,flavonoids,tannins,vitamin E, β-carotene and antioxidant activity during soaking of three white sorghum varieties.Methods: The changes in total phenols,total ilavonoids,tannins,phenolic acids compounds,flavonoid components,vitamin E,P-carotene and antioxidant activity during soaking of sorghum grains were determined.Results:Total phenols,total flavonoids,tannins,vitamin E,P-carotene and antioxidant activity in raw sorghum were ranged from 109.21 to 116.70,45.91 to 54.69,1.39 to 21.79 mg/100 g,1.74 to 5.25,0.54 to 1.19 mg/kg and 21.72%to 27.69%and 25.29%to 31.97%,respectively. The above measured compounds were significantly decreased after soaking.p-Hydroxybenzoic acid,vanillic acid,syringic acid and cinnamic acid represent the major phenolic acids in Dorado variety.While ferulic acid,p-coumaric acid,gallic acid and caffeic acid represent the major phenolic acids in Shandaweel-6.On the other hand,protocatechuic acid represents the major phenolic acids in Giza-15.Regarding flavonoids components,Dorado was the highest variety in kampferol and naringenin while Shandaweel-6 was the highest variety in luteolin, apigenin,hypersoid,quercelin and christen.Finally,Giza-15 was the highest variety in catechin. Phenolic acids,flavonoid compounds and antioxidant activities were decreased after soaking. Conclusions:Sorghum varieties have moderate quantities from total phenols,total flavonoids, tannins,phenolic acids compounds,flavonoid components,vitamin E,P-carotene and antioxidant activity which decreased after soaking. | Abd El-Moneim MR Afify Hossam S El-Beltagi Samiha M Abd El-Salam Azza A Omran | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，3： | 7 |
| 2 | Biochemical changes in grape rootstocks resulted from humic acid treatments in relation to nematode infection显示文摘Objective:To investigate the effect of humic acid on nematode infected,resistant and susceptible grapes in relation to lipid peroxidation and antioxidant mechanisms on selected biochemical parameters known as proactive substances.Methods:The grape rootstocks,superior,superior/ freedom and freedom were reacted differently to Meloidogyne incognita and Rotylenchulus reniformis according to rootstock progenitor.Two weeks after inoculation,two commercial products of humic acid were applied at the rate of(2,4 mL or grams/plant) as soil drench.After 4 months,nematode soil populations were extracted and counted.A subsample of roots from each plant was stained and gall numbers,embedded stages per root were calculated,final population, nematode build up(Pf/Pi),average of eggs/eggmass were estimated.Subsamples of fresh root of each treatment were chemically analyzed.Results:Freedom reduced significantly the nematode criteria and build up.Humic acid granules appeared to be more suppressive to nematode build up on superior and the higher dose on superior/freedom than liquid treatments.On freedom,all treatments reduced significantly the nematode build up regardless to the material nature.The higher dose was more effective than the lower one.As a result of humic acid applications,the malondialdehyde(MDA) and H_2O_2 contents were significandy reduced after humic acid treatments while the antioxidant compounds glutathione(GSH),ascorbic acid(ASA) and total phenol contents were significandy increased when compared with check.Antioxidant defense enzymes ascorbate peroxidase(AFX),superoxide dismutase(SOD),catalase(CAT) and polyphenol oxidase(PPO) showed significant increase in their specific activities in treated plants compared with nematode treated check.Conclnsions:Humic acid treatments improve we yield of grape by increasing the contents of antioxidant compounds and the specific activities of antioxidant enzymes. | Hosny H Kesba Hossam S El-Beltagi | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，4： | 4 |
| 3 | Control of Tetranychus urticae Koch by extracts of three essential oils of chamomile,marjoram and Eucalyptus显示文摘Objective:To evaluate the acaricidal activity of extracts of three essential oils of chamomile,marjoram and Eucalyptwi against Tetranychus urtiaie(T.urticae.)Koch.Methods:Extracts of three essential oils of chamomile,marjoram and Eucalyptus with different concentrations(0.5%,1.0%,2.0%,3.0%and 4.0%)were used to control T.urticae Koch.Results:The results showed that chamomile(Chamomilla recutita)represented the most potent efficient acaricidal agent against Tetranychus followed by marjoram(Marjorana hortensis)and Eucalyptus.The LC_(50)values of chamomile,marjoram and Eucalyptus for adults were 0.65,1.84 and 2.18,respectively and for eggs 1.17,6.26 and 7.33,respectively.Activities of enzymes including glutathione-Stxansferase,esterase(α-esterase andβ-esterase)and alkaline phosphatase in susceptible mites were determined and activities of enzymes involved in the resistance of acaricides were proved.Protease enzyme was significantly decreased at LC_(50)of both chamomile and marjoram compared with positive control.Gas chromatography-mass spectrometer(CC-MS)proved that the major compositions of Chamomilla recutita areα-bisabolol oxide A(35.251%),and trans-3-farersene(7.758%),while the main components of Marjorana hortensis are terpincne-4-ol(23.860%),p-cymene(23.404%)and sabinene(10.904%).Conclusions:It can be concluded that extracts of three essential oils of chamomile,marjoram and Eucalyptus possess acaricidal activity against T.urticae. | Abd El-Moneim MR Afify Fatma S Ali Turky AF | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，1： | 2 |
| 4 | Effect of lead acetate toxicity on experimental male albino rat显示文摘Objective:To evaluate the effect of different doses of lead acetate(1/20,1/40 and 1/60 of LD_(50))on body weight gain,blood picture,plasma prolein profile and the function of liver,kidney and thyroid gland.Methods:Male albino rats were divided into four groups,the first group represented the health control animals,while the second,third and fourth groups were ingested orally with sub lethal doses of load acetate(1/20,1/40 and 1/60)of the oral LD_(50),respectively.One dose was ingested every two days during the experimental period(14 weeks)including the adaptation time.Blood was collected and used for all analysis.Results:The results showed that,the ingestion of Pb^(2+)induced significant stimulation in glutamic-pyruvic transaminase(ALT)and glutamic-oxalacetic transaminease(AST)activity.Also,total soluble protein and albumin contents of plasma were significantly decreased,while the content of globulin was changed by the Pb^(2+)treatments.The cholinesteiase activity was inhibited,but the activities of alkaline and acid phosphates and lactate dehydrogenase were stimulated,while plasma glucose level was elevated as a result of lead acetate intoxication.In case of blood picture,Pb^(2+)ingestion reduced the contents of hemoglobin and RBCs count of intoxicated rat's blood and the plasma levels of T3,T4 and blood WBCs count were decreased.Conclusions:It can be concluded that lead acetate has harmful effect on experimental male albino rats.Therefore,the present work advises people to prevent exposure to the lead compound to avoid injurious hazard risk. | Nabil M Ibrahim Esam A Eweis Hossam S El-Beltagi Yasmin E Abdel-Mobdy | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，1： | 1 |
| 5 | 不同方法提取乌墨叶挥发油化学成分的研究显示文摘目的研究乌墨叶挥发油的化学成分。方法采用水蒸气蒸馏与超声波萃取分别提取乌墨叶挥发油,运用毛细管气相色谱-质谱联用法结合计算机检索对其挥发油化学成分进行了分析。结果显示从水蒸汽蒸馏法和超声波萃取所得挥发油中分别鉴定出了34和37种化合物。用面积归一法测定了2种挥发油中各种成分的相对百分含有量,各占总峰面积的97.31%和98.19%。2种挥发油化学组成各有异同,其主要成分为α-蒎烯、β-杜松烯、α-古芸烯、丁香烯、α-蛇麻烯、β-芹子烯、异长叶烯-5-酮、韦得醇、1,4a-二甲基-7-异丙烯基-4,4a,5,6,7,8-六氢-萘-2-酮。结论不同提取方法所得乌墨叶挥发油化学成分存在差异,但主要成分基本相同。 | 刘艳清 汪洪武 蔡璇 | 2014 | 中成药2014，36，5： | 1 |
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