| 1 | Free radical scavenging activity of three different flowers-Hibiscus rosa-sinensis,Quisqualis indica and Senna surattensis显示文摘Objective:To evaluate three flowers of Hibiscus rosa-sinensis(H.rosa-sinensis),Quisqualis indica(Q.indica) and Senna surattensis(S.surattensis) for their antioxidant activity by different methods in addition to total phenolic,flavonoid and pigment contents.Methods:Antioxidant activity of water,ethanol and absolute ethanol extracts of three flowers; H.rosa-sinensis,Q.indica and S.surattensis was evaluated.The antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity,ferrous chelating activity,reducing power,nitric oxide scavenging activity,hydroxyl radical scavenging activity as well as total antioxidant capacity.Total flavonoids,total phenols and total pigments including chlorophylls and carotenoids were measured for the three flowers.Results:The results showed that the highest total antioxidant capacity at concentration of 500 mg/L was found in S.surattensis as 0.479 ± 0.001.Scavenging activity of H.rosasinensis,Q.indica and S.surattensis flower extracts against 1,1-diphenyl-2-picrylhydrazyl radical showed the highest activity of(90.20 ± 0.29)% with 500 mg/L.Phytochemical screening of the three flowers extracts were carried out for alkaloids,flavonoids,saponins,tannins,steroids,glycosides,terpenoids,amino acid and mucilages.H.rosa-sinensis showed the total phenolic in water extract of(235.77 ± 14.31) mg/100 g,the other two flowers Q.indica and S.surattensis had the total phenolic in ethanol extracts of(937.70 ± 25.06) and(850.30 ± 13.81) mg/100 g,respectively.On the other hand total flavonoids were identified in absolute ethanol extracts in the three flowers[(32.83 ± 1.34),(49.24 ± 4.87) and(2.79 ± 0.23) mg/100 g,respectively].Conclusions:The extracts in the constituents of the three flowers could be used as additives as supplement fractions in foods. | Abd El-Moneim Mohamed Radwan Afify Hazem Mohamed Mahmoud Hassan | 2016 | Asian Pacific Journal of Tropical Biomedicine2016,6,9: | 1 |
| 2 | Antioxidant antileishmanial cytotoxic and antimicrobial activities of a local plant Myrtus nivellei from Algeria Sahara显示文摘Objective: To study the phytochemical constituents and in vitro biological activities of hydromethanolic extract and fractions from Algerian Sahara Myrtus nivellei(M. nivellei)collected in Hoggar region and to identify the active fraction that can act as an alternative of commonly used antibiotics and as antileishmanial or antioxidant agents.Methods: Phytochemical screening of M. nivellei aerial parts was realised according to the literature. Extract was firstly prepared by using aqueous methanol then fractionated with ethyl acetate and butanol solvents. Total phenolics, tannis and flavonoids, of the hydromethanolic extract and their fractions were determined by Folin-Ciocalteu method as gallic acid equivalents and by aluminium chloride as rutin equivalent respectively.Extract and fractions were tested for their antimicrobial and antiparasital activities against standard bacteria using agar diffusion method and two kinds of leishmania visceral and cutaneous. The antioxidant activities were realized using phosphomolybdenum, FRAP and DPPH tests.Results: Preliminary phytochemical screening exhibited the presence of flavonoids,tannins, saponins, and alkaloids. The experimental results showed that plant extract and fractions were high in phenolic compounds and exhibited an important role as antioxidant,antimicrobial and had a moderate antileishmanial activity.Conclusions: These observations lead us toward more studies in this field, so that we can get more benefits from our local Algerian medicinal plants. | Farah Ramdane Rym Essid Nadia Fares Dahmane El Ouassis Sana Aziz Mahfoud Hadj Mahammed Mohamed Didi Ould Hadj Ferid Limam | 2017 | Asian Pacific Journal of Tropical Biomedicine2017,7,8: | 1 |