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| 1 | Larvicidal activity of Xenorhabdus and Photorhabdus bacteria against Aedes aegypti and Aedes albopictus显示文摘Objective: To evaluate the efficacy of symbiotic bacteria, Xenorhabdus indica, Xenorhabdus stockiae, Photorhabdus luminescens subsp. akhurstii and Photorhabdus luminescens subsp. hainanensis as a larvicide against Aedes aegypti and Aedes albopictus. Methods: Larvae(L3-L4) of Aedes aegypti and Aedes albopictus were given 2 m L of a suspension 107-108 CFU/m L of each symbiotic bacterium. Distilled water and Escherichia coli ATCC襅25922 were used as the control. The mortality rate of the larval mosquitoes was observed at 24, 48, 72 and 96 h. The experiment was performed in triplicates. Results: The larvae of both Aedes species started to die at 24 h exposure. Aedes aegypti showed the highest mortality rate(87%-99%), 96 h after exposure to Xenorhabdus stockiae(b NBP22.2_TH). The mortality rate of Aedes albopictus was between 82% and 96% at 96 h after exposure to Xenorhabdus indica(b KK26.2_TH). Low effectiveness of distilled water and Escherichia coli ATCC襅25922 were observed in both Aedes larvae, with a mortality rate of 2% to 12%. Conclusions: The study confirms the oral toxicity of Xenorhabdus and Photorhabdus bacteria against Aedes spp. Xenorhabdus stockiae and Xenorhabdus indica may be an alternative agent for control Aedes spp. This is basic information for further study on the mechanism of action on Aedes larvae or application to control mosquito larvae in the community. | Apichat Vitta Punnawat Thimpoo Wipanee Meesil Thatcha Yimthin Chamaiporn Fukruksa Raxsina Polseela Bandid Mangkit Sarunporn Tandhavanant Aunchalee Thanwisai | 2018 | Asian Pacific Journal of Tropical Biomedicine2018，8，1： | 0 |
| 2 | 不同温度下苏云金杆菌对白纹伊蚊的杀虫效果研究显示文摘目的探讨在不同环境温度下苏云金杆菌以色列亚种(Bacillus thuringiensisisraelensis,Bti)对白纹伊蚊幼虫的杀伤效果。方法采用Probit analysis生物测定法,检测和比较分析不同环境温度下Bti对白纹伊蚊三龄幼虫的杀伤效果。利用不同浓度Bti处理后的幼虫累积死亡率对应P值和浓度对数值进行线性回归分析,获得线性回归公式,计算LC50和LC95等重要剂量参数。结果Bti对白纹伊蚊幼虫具有明显的杀伤效应,药物起效快。在常规28℃,Bti处理后12 h即出现明显的幼虫死亡,随Bti处理时间的延长和浓度的增大,白纹伊蚊幼虫累积死亡率升高。本研究获得了不同温度下各时间点Bti对白纹伊蚊幼虫毒杀作用的线性回归公式。同一Bti浓度下28℃~32℃的杀虫效果较为理想,20℃的低温度环境下Bti杀虫效果相对最弱;28℃下各时间点的LC95均最低,在24 h、48 h、72 h和96 h的LC95分别为122.418、109.404、96.474和92.229 IU/L。结论Bti对白纹伊蚊三龄幼虫具有很好的杀伤效果,环境温度可影响Bti对白纹伊蚊的杀虫效果,在一定范围内适当升高温度可以增强其杀灭效果;在Bti的实际使用中,应充分考虑温度因素的影响,以达到更好的杀虫效果。 | 刘婷婷 王英 郑丹 刘治隆 李昕 王静 于莎莎 | 2023 | 中国热带医学2023，23，12： | 0 |
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