| 1 | 玉郎伞多糖对双氯芬酸致小鼠肝损伤的保护作用及相关机制研究显示文摘目的:探讨玉郎伞多糖(Yulangsan polysaccharide,YLSPS)对双氯芬酸(Diclofenac,DICF)致小鼠肝损伤的保护作用及相关机制研究。方法:把昆明种小鼠随机分为正常对照组(normal control,NC),DICF损伤模型组,YLSPS高(YLSPSH,600 mg/kg)、YLSPS中(YLSPSM,300 mg/kg)、YLSPS低(YLSPSL,150 mg/kg)剂量组和联苯双酯阳性对照组(dimethyl diphenyl bicarboxylate,200 mg/kg DDB),每组10只。YLSPS高、中、低剂量组和联苯双酯组灌胃给予相应药物,正常对照组和模型组同法给予相等容积的生理盐水,每日1次,连续14 d。末次给药后1 h,除正常组给予相等容量生理盐水腹腔注射外,其余各组小鼠同法给予DICF 50 mg/kg。造模后动物禁食20 h,取血及脏器检测肝功能指标碱性磷酸酶(alkaline phosphatase,ALP)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)及总胆红素(total bilirubin,TBIL),检测肝匀浆超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、丙二醛(malondialdehyde,MDA)和过氧化氢酶(catalase,CAT)的水平及血清中白介素-1β(interleukin-1β,IL-1β)水平,HE(Hematoxylin eosin)染色法观察各组小鼠肝细胞损伤程度。结果:与模型对照组比较,YLSPS能降低血清ALT、AST、ALP活性和TBIL含量以及肝组织MDA含量(P<0.05),能降低血清TNF-α、IL-1β水平(P<0.05),提高肝组织SOD、GSH-Px以及CAT活性(P<0.05)。结论:YLSPS能够有效减轻肝细胞损伤程度,对DICF所致的小鼠肝损伤具有保护作用,机制可能与抗氧自由基及抑制脂质过氧化等作用有关。 | 阮文福 梁杏梅 段文明 陈春霞 黄仁彬 | 2015 | 重庆医科大学学报2015，40，10： | 4 |
| 2 | Synergistic anticancer effect of the extracts from Polyalthia evecta caused apoptosis in human hepatoma(HepG2)cells显示文摘Objective:To evaluate the anticancer activity of the extract fraction of Polyalthia evecta(P. evecta)(Pierre) Finet & Gagnep and the synergistic anticancer effect of the extracts from P.evecta by using the ATR/FT-IR spectroscopy.Methods:The 50%ethanol-water crude leaf extract of P.evecta(EW-L) was prepared and was further fractionated to isolate various fractions.The anticancer activity was investigated from cytotoxicity against HepG2 using a neutral red assay and apoptosis induction by evaluation of nuclei morphological changes after DAPI staining. Synergistic anticancer effects of the extracts from P.evecta were performed using the ATR/FTIR spectroscopy.Results:The result showed that the EW-L showed higher cytotoxicity and apoptosis induction in HepG2 cells than its fractionated extracts.The hexane extract exhibited higher cytotoxicity and apoptosis induction than the water extracts,but less than the EW-L. The combined water and hexane extracts apparently increased cytotoxicity and apoptosis induction.The%apoptotic cells induced by the extract mixture were increased about 2-fold compared to the single hexane extract Conclusions:The polar extract fraction is necessary for the anticancer activity of the non-polar extract fraction.The ATR/FT-IR spectra illustrates the physical interaction among the constituents in the extract mixture and reveals the presence of polyphenolic constituents in the EW-L,which might play a role for the synergistic anticancer effect. | Sasipawan Machana Natthida Weerapreeyakul Sahapat Barusrux Kanjana Thumanu Warapom Tanthanuch | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，8： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员