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| 1 | Evaluating the effectiveness of marine actinobacterial extract and its mediated titanium dioxide nanoparticles in the degradation of azo dyes显示文摘Aim of the present study was to synthesize titanium dioxide nanoparticles(TiO2 NPs) from marine actinobacteria and to develop an eco-friendly azo-dye degradation method. A total of five actinobacterial isolates were isolated from Chennai marine sediments, Tamilnadu, India and analyzed for the synthesis of TiO2 NPs using titanium hydroxide. Among these, the isolate PSV 3 showed positive results for the synthesis of TiO2 NPs, which was confirmed by UV analysis. Further characterization of the synthesized TiO2 NPs was done using XRD, AFM and FT-IR analysis. Actinobacterial crude extract and synthesized TiO2 NPs was found efficient in degrading azo dye such as Acid Red 79(AR-79) and Acid Red 80(AR-80). Degradation percentage was found to be 81% for AR-79, 83% for AR-80 using actinobacterial crude extract and 84% for AR-79, 85% for AR-80 using TiO2 NPs. Immobilized actinobacterial cells showed 88% for AR-79 and 81% for AR-80, dye degrading capacity. Degraded components were characterized by FT-IR and GC-MS analysis. The phytotoxicity test with 500 μg/mL of untreated dye showed remarkable phenotypic as well as cellular damage to Tagetes erecta plant. Comparatively no such damage was observed on plants by degraded dye components. In biotoxicity assay, treated dyes showed less toxic effect as compared to the untreated dyes. | S Priyaragini S Veena D Swetha L Karthik G Kumar K V Bhaskara Rao | 2014 | Journal of Environmental Sciences2014，26，4： | 4 |
| 2 | 产抗氧化活性物质的海洋放线菌筛选与鉴定显示文摘采用传统的分离培养技术对4种海洋动物肠道中的放线菌进行分离,利用紫外-可见分光光度法对分离菌株的代谢产物进行抗氧化活性初筛测定,再采用电子自旋共振仪(ESR)进行4种自由基清除率复筛测定,并对抗氧化活性强的菌株进行16S r DNA水平鉴定与系统发育树分析。结果表明共分离到37株放线菌,初筛测定获得7株菌的代谢产物对DPPH自由基清除率超过50%,其中菌株CH16与CH38的代谢产物通过ESR测定发现对DPPH自由基、羟基自由基、超氧阴离子自由基及烷基自由基的消除率均超过50%,并分别鉴定为Amycolatopsis rifamycinica与Amycolatopsis sp.。该研究为从海洋环境获得产生抗氧化活性物质菌株资源与开发提供依据。 | 秦曼曼 张雪梅 王晓杰 冯紫君 刘颖 千忠吉 王雅玲 | 2016 | 食品工业科技2016，37，5： | 1 |
| 3 | 一株耐热珊瑚共附生真菌的鉴定及抗氧化活性的研究显示文摘珊瑚共附生真菌及其代谢产物研究活跃,产物种类多样,活性丰富,但珊瑚中的耐热真菌未见报道。对耐热珊瑚共附生真菌C21-5B进行鉴定,研究其生理学特性及代谢产物的抗氧化活性,为该菌的深入研究及利用提供依据。通过形态学观察、18S rRNA序列分析鉴定菌株;通过测量不同海精盐浓度、pH、温度条件下的菌落直径研究该菌生理特性;根据DPPH自由基清除率、ABTS自由基清除率测定该菌抗氧化活性;通过薄层层析和高效液相色谱分析代谢产物活性成分。珊瑚真菌C21-5B经鉴定为土曲霉(Aspergillus terreus)。生理特性研究表明,菌株C21-5B能够生长的条件:海精盐浓度0.0%~20.0%、pH值2.0~12.0、温度15~45℃;最适生长条件:海精盐浓度0.5%~3.5%、pH值6.0~8.0、温度35℃。该菌代谢产物的乙酸乙酯萃取物具有较高的抗氧化活性,DPPH和ABTS自由基的EC50分别为0.113、0.504 mg/mL。经薄层层析和高效液相色谱分析,发现该菌代谢产物的中性极性成分偏多,含有生物碱、类脂化合物、还原性物质、黄酮类物质、芳香胺类。菌株C21-5B为耐热、兼性海洋真菌,具有极强的耐酸碱能力,代谢产物种类丰富,并具有明显的抗氧化活性,具有开发利用潜力。 | 顾哲铭 杨永星 黄晓欣 李雪珽 谈嘉莉 雷晓凌 聂芳红 | 2023 | 微生物学杂志2023，43，2： | 0 |
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