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| 1 | 隔药灸对克罗恩病肠纤维化大鼠结肠成纤维细胞CTGF、FN、Smad表达的影响显示文摘目的观察隔药灸对克罗恩病肠纤维化大鼠结肠成纤维细胞(conlonic fibroblast,CFB)CTGF、FN蛋白和Smad3、Smad7 mRNA表达的影响,探讨隔药灸治疗克罗恩病肠纤维化的作用机制。方法 SD大鼠随机分为正常组、模型组、隔药灸组和西药组。在成功制备克罗恩病肠纤维化大鼠模型的基础上,隔药灸组取天枢穴(双)、气海穴进行治疗,西药组以美沙拉嗪灌胃治疗。各组治疗干预结束后,留取结肠组织,分离并体外培养大鼠CFB。采用免疫细胞化学法观察各组大鼠CFB的CTGF、FN蛋白表达,应用实时荧光定量PCR技术检测各组大鼠CFB的Smad3、Smad7 mRNA表达。结果与正常组比较,模型组大鼠CFB的CTGF、FN蛋白表达升高(均P<0.01);与模型组比较,隔药灸组CFB的CTGF、FN蛋白表达降低(均P<0.01)。各组大鼠比较,CFB的Smad3和Smad7 mRNA表达变化均不显著(均P>0.05)。结论隔药灸能下调克罗恩病肠纤维化大鼠肠成纤维细胞CTGF、FN的蛋白表达。 | 安彩萍 黄燕 马晓芃 吴焕淦 施征 杨玲 窦传字 洪珏 | 2014 | 上海针灸杂志2014，33，6： | 9 |
| 2 | Increased duodenal expression of mi R-146a and-155 in pediatric Crohn's disease显示文摘AIM: To evaluate the role of micro RNA(mi R)-146 a,-155 and-122 in the duodenal mucosa of pediatric patients with Crohn's disease(CD) and the effect of transforming growth factor-β(TGF-β) on these mi Rs in duodenal epithelial and fibroblast cells.METHODS: Formalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed(CD inflamed: n = 10) and intact(CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children(C: n = 10) were examined. Expression of mi R-146 a,-155 and-122 was determined by realtime polymerase-chain reaction(PCR). The expression of the above mi Rs was investigated in recombinant human TGF-β(1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells(CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.RESULTS: Expression of mi R-146 a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD(CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, p ≤ 0.01) and to the control group(CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, p ≤ 0.05). The expression of mi R-155 was significantly increased in the inflamed region of the duodenum compared to the control group(CD inflamed: 4.87 ± 1.02 vs Control: 1.00 ± 0.40, p ≤ 0.001). The expression of mi R-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of mi R-155 in small intestinal epithelial cells(TGF-β: 0.7 ± 0.083 vs Control: 1 ± 0.09, p ≤ 0.05) and also the expression of mi R-146a(TGF-β: 0.67 ± 0.04 vs Control: 1 ± 0.15, p ≤ 0.01) and mi R-155(TGF-β: 0.72 ± 0.09 vs Control: 1 ± 0.06, p ≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of mi R-122.CONCLUSION: The elevated expression of mi R-146 a and-155 in the inflamed duodenal mucosa of CD patients suggests the role of these mi Rs in the pathomechanism of inflammatory bowel disease. Antiinflammatory TGF-β plays an important role in the regulation of the expression of these mi Rs. | Dániel Szucs Nóra Judit Béres Réka Rokonay Kriszta Boros Katalin Borka Zoltán Kiss András Arató Attila J Szabó ádám Vannay Erna Sziksz Csaba Bereczki Gábor Veres | 2016 | World Journal of Gastroenterology2016，22，26： | 2 |
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