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| 1 | Authentication and distinction of Shenmai injection with HPLC fingerprint analysis assisted by pattern recognition techniques显示文摘In this paper,the feasibility and advantages of employing high performance liquid chromatographic(HPLC) fingerprints combined with pattern recognition techniques for quality control of Shenmai injection were investigated and demonstrated.The Similarity Evaluation System was employed to evaluate the similarities of samples of Shenmai injection,and the HPLC generated chromatographic data were analyzed using hierarchical clustering analysis(HCA) and soft independent modeling of class analogy(SIMCA).Consistent results were obtained to show that the authentic samples and the blended samples were successfully classified by SIMCA,which could be applied to accurate discrimination and quality control of Shenmai injection.Furthermore,samples could also be grouped in accordance with manufacturers.Our results revealed that the developed method has potential perspective for the original discrimination and quality control of Shenmai injection. | Xue-Feng Lu Kai-Shun Bi Xu Zhao Xiao-Hui Chen | 2012 | Journal of Pharmaceutical Analysis2012，2，5： | 5 |
| 2 | 基于LC-MS/MS分析不同蛋白酶酶解奶牛胎盘产物差异及其抗氧化活性预测显示文摘为探究胰蛋白酶、胃蛋白酶和木瓜蛋白酶酶解奶牛胎盘产物间差异,用此3种酶在最优条件下酶解奶牛胎盘获得胎盘酶解产物,每种酶重复3次,利用LC-MS/M技术鉴定产物中蛋白和肽段,BIO-PEP数据库匹配和测算十肽及以下的抗氧化活性多肽。结果显示:胰蛋白酶、胃蛋白酶和木瓜蛋白酶酶解产物分别鉴定出541、137和86种蛋白,2766、1170和128个肽段,胰蛋白酶鉴定蛋白和肽段数极显著高于其余2种酶(P<0.01),木瓜蛋白酶鉴定蛋白和肽段数均显著低于其余2种酶(P<0.05);3种酶酶解产物十肽及以下具有潜在抗氧化能力肽段分别占十肽及以下肽段总量的39.14%(256/654)、32.80%(81/247)和53.33%(16/30),木瓜蛋白酶占比显著高于其余2种酶(P<0.05);胰蛋白酶、胃蛋白酶和木瓜蛋白酶3种酶酶解产物抗氧化多肽共同特征是分别含有EL、LY、MM、LK、IR、MY、VY残基,HL、LK、TY、HH、EL残基和LK、IR、EL等残基。综上,通过LC-MS/M技术对酶解产物定性和定量分析,胰蛋白酶酶解奶牛胎盘的产物生物学信息最丰富,在蛋白组学中可靠性和准确性最高;木瓜蛋白酶酶解奶牛胎盘最彻底,其产物潜在抗氧化活性最强,本研究为使用蛋白组学研究奶牛胎盘时酶的选择和奶牛胎盘抗氧化活性多肽的开发利用提供理论依据。 | 张钺 尤留超 朱颍琨 莘余 吕尚揆 裴淼毓 傅宏庆 沈留红 | 2021 | 中国农业大学学报2021，26，11： | 1 |
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