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| 1 | 大鼠发育过程中脊髓组织CAPON与Dexras1 mRNA表达的实验研究显示文摘为探讨大鼠发育过程中神经型一氧化氮合酶羧基末端PSD-95/DLG/ZO-1(PDZ)结合配体(carboxy-terminal PDZ ligandof nNOS,CAPON)与Dexras1 mRNA的表达及二者的相关性,本实验采用大鼠发育模型,通过实时荧光定量聚合酶链式反应(Realtime-PCR)及原位杂交组织化学(ISH)与免疫组织化学相结合的技术检测脊髓组织中CAPON及Dexras1 mRNA的表达变化。结果表明:在胚胎14~18d的脊髓组织中,CAPON mRNA呈低水平表达,生后1d表达升高并达到高峰。从形态上看,表达于尚未分化成熟的前角,随后呈逐渐下降趋势。在生后12w,表达于脊髓后角。而Dexras1 mRNA在胚胎14d呈低水平表达,16~18d时表达升高,同样在生后1d出现表达高峰并定位于尚未分化成熟的前角,随后表达逐渐下降。生后12w,在后角有表达。根据Real-time PCR结果作直线相关分析,在生后脊髓发育过程中CAPON与Dexras1 mRNA表达呈高度相关,于生后1d共定位于脊髓前角。形态学结果显示CAPON与神经型一氧化氮合酶(nNOS)表达于相同细胞,但亚细胞定位不同。本研究表明,大鼠发育过程中,脊髓组织中CAPON及Dexras1 mRNA存在共表达,提示CAPON可能通过调节nNOS,进而激活Dexras1,在神经元的分化、突触的可塑性及突触发生过程中发挥一定的作用。 | 李欣 程纯 赵剑 陈梦玲 牛淑琼 高尚峰 沈爱国 | 2007 | 神经解剖学杂志2007，23，4： | 2 |
| 2 | Yanda Li:A never-stopping pioneer,investigator and founder显示文摘Yanda Li was bom in October 1936 in Dongguan,Guangdong,China.His childhood was in war time.He had to move with his parents across several counties and cities,but he managed to accomplish his high-school study in Guangzhou(Fig.1).He showed deep interest in physics in high school and was admitted to the Department of Electrical Engineering of Tsinghua University in 1954.Because of his excellent performance in college study,he joined the faculty in 1958 before he finished his college study in 1959.Throughout the 60 years from Figure 1.Yanda Li in his high-school age.Credit:Yanda Li,unknown photographer. | Xuegong Zhang Jin Gu | 2022 | Quantitative Biology2022，10，1： | 0 |
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