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1一株富集铯的微生物及其在植物修复中的应用显示文摘利用一株分离自重金属污染土壤的伯克霍尔德菌(Burkholderia),研究其对放射性核素铯的耐性、富集,并在水培籽粒苋(Amaranthus cruentus L)并接种伯克氏菌,研究接种伯克氏菌对籽粒苋生长和吸收铯的影响。结果表明:伯克氏菌D54在铯浓度达到50 mmol.L-1的培养基中能正常生长,含铯1 mmol.L-1的100 mL液体培养基中接种1%活化菌液培养3 d后,收集到0.173 7 g干重菌体,菌体铯的质量分数达到45 mg.g-1,培养基中铯的去除率达58.77%。接种伯克氏菌促进籽粒苋生长,在Cs浓度分别为0、200、500、1 000μmol.L-1条件下,籽粒苋地上部的生物量分别增加30.91%,9.29%,42.71%,74.50%,根的干物质量分别增加36.01%、62.89%、55.17%、63.50%。籽粒苋的根、茎、叶中铯含量分别增加27.94%~43.58%、14.9~34.51%、6.31%~11.48%。接种伯克氏菌籽粒苋的耐受指数(TI%)增加11%~28%,生物富集率(BR)增加5%~9%。廖上强 郭军康 宋正国 唐世荣 2011生态环境学报2011,20,4:8
2西藏搭格架铯硅华区热泉高温菌株的分离及特征研究显示文摘从西藏搭格架铯硅华矿床区热泉中分离培养高温菌T4-1,并进行了格兰氏染色、显微镜观察、室内温度实验、16SrRNA基因分析等。结果表明,T4-1为杆状菌,格兰氏染色阳性,其生长范围为45-80℃,最适生长温度70℃。16SrRNA基因分析结果表明,该菌株属于地芽孢杆菌属(Geobacillus),在发育树上,T4-1菌株与高温烷烃地芽孢杆菌(Geobacillus the rmoleovorans)非常近。本研究为进一步开展西藏高温微生物资源以及微生物参与成矿作用的研究提供了首例。孔凡晶 王海雷 郑绵平 郑小娟 2007地质学报2007,81,12:3
3Cesium accumulation by bacterium Thermus sp. TibetanG7:hints for biomineralization of cesium-bearing geyserite in hot springs in Tibet,China显示文摘The bacterium Thermus sp. TibetanG7, isolated from hot springs in Tibet, China, was examined for the ability to accumulate cesium from solutions. Environmental conditions were simulated and the effects of pH, K+, Na+ and K+-regimes were then studied to determine the possible role of the bacterium in the formation of cesium-bearing geyserite around these hot springs. In despite of the inhibition of K+ and Na+, the bacterium Thermus sp. TibetanG7 revealed noticeable accumulation of cesium from solutions, with maximum accumulations of 53.49 and 40.41 μmol Cesium/g cell dry weight in Na+ and K+ inhibition experiments, respectively. The accumulation of cesium by this microorganism is rapid, with 40%―50% accumulated within the first 5 min. K+-deficient cells showed a much higher capacity of cesium accu- mulation compared with K+-sufficient cells. It is evident that the bacteria within the genus thermus play a significant role in the cesium assembly. The formation of cesium-bearing geyserite is also considered.WANG HaiLei1,2,3, ZHENG MianPing1,2,3 & HUANG XiaoXing4 1 Institute of Mineral Resources, Chinese Academy of Geological Sciences (CAGS), Beijing 100037, China 2 R&D Center for Saline Lake and Epithermal Deposits, CAGS, Beijing 100037, China 3 Open Laboratory of Saline Lake Resourses and Environment, CAGS, Beijing 100037, China 4 Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100081, China 2007Chinese Science Bulletin2007,52,19:2
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