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| 1 | Identification of squamous cell carcinoma associated proteins by proteomics and loss of beta tropomyosin expression in esophageal cancer显示文摘AIM: To assess the proteome of normal versus tumor tissue in squamous cell carcinoma of the esophagus (SCCE) in Iranian patients and compare our results with former reports by using proteomics. METHODS: Protein was extracted from normal and tumor tissues. Two dimensional electrophoresis was carried out and spots with differential expression were identified with mass spectrometry. RNA extraction and RT-PCR along with immunodetection were performed. RESULTS: Fourteen proteins were found whose expression levels differed in tumor compared to normal tissues. Mass spectrometric analysis resulted in the identification of β-tropomyosin (TMβ), myosin light chain 2 (and its isoform), myosin regulatory light chain 2, peroxyredoxin 2, annexinⅠand an unknown polypeptide as the down regulated polypeptides in tumor tissue. Heat shock protein 70 (HSP70), TPM4-ALK fusion oncoprotein 2, myosin light polypeptide 6, keratinⅠ, GH16431p and calreticulin were the up-regulated polypeptides found in tumor tissue. Several of these proteins, such as TMβ,HSP70, annexinⅠ, calreticulin, TPM4-ALK and isoforms of myosins, have been well recognized in tumorigenesis of esophageal or other types of cancers. CONCLUSION: Our study not only supports the involve- ment of some of the formerly reported proteins in SCCE but also introduces additional proteins found to be lost in SCCE, including TMβ. | Ferdous Rastgar Jazii Zahra Najafi Reza Malekzadeh Thomas P Conrads Abed Ali Ziaee Christian Abnet Mansour Yazdznbod Ali Asghar Karkhane Ghasem H Salekdeh | 2006 | World Journal of Gastroenterology2006，12，44： | 14 |
| 2 | Differential gene expression between squamous cell carcinoma of esophageus and its normal epithelium; altered pattern of mal,akrlc2, and rablla expression显示文摘AIM: To identify the altered gene expression patterns in squamous cell carcinoma of esophagus (ESCC) in relation to adjacent normal esophageal epithelium. METHODS: Total RNA was extracted using SV total RNA isolation kit from snap frozen tissues of ESCC samples and normal esophageal epithelium far from the tumor. Radio-labeled cDNA were synthesized from equal quantities of total RNAs of tumor and normal tissues using combinations of 24 arbitrary 13-mer primers and three different anchoring oligo-dT primers and separated on sequencing gels. cDNA with considerable different amounts of signals in tumor and normal tissue were reamplified and cloned. Using southern blot, the clones of each band were controlled for false positive results caused by probable heterogeneity of cDNA population with the same size. Clones that confirmed differential expression by slot blot selected for sequencing and northern analysis. Corresponding full-length gene sequences was predicted using human genome project data, related transcripts were translated and used for various protein/motif searches to speculate their probable functions. RESULTS: The 97 genes showed different levels of cDNA in tumor and normal tissues of esophagus. The expression of mal gene was remarkably down. regulated in all 10 surveyed tumor tissues. Akrlc2, a member of the aldoketo reductase 1C family, which is involved in metabolism of sex hormones and xenobiotics, was up-regulated in 8 out of 10 inspected ESCC samples. Rablla, RPL7, and RPL28 showed moderate levels of differential expression. Many other cDNAs remained to further studies. CONCLUSION: The mal gene which is switched-off in all ESCC samples can be considered as a tumor suppressor gene that more studies in its regulation may lead to valuable explanations in ESCC development. Akrlc2 which is upregulated in ESCC probably plays an important role in tumor development of esophagus and may be proposed as a potential molecular target in ESCC treatments. Differential display technique in spite of many disadvantages is still a valuable technique in gene function exploration studies to find new candidates for improved ones like gene chips. | Sakineh Kazemi-Noureini Sergio Colonna-Romano Abed-Ali Ziaee Mohammad-Ali Malboobi Mansour Yazdanbod Parviz Setayeshgar Bruno Maresca | 2004 | World Journal of Gastroenterology2004，10，12： | 4 |
| 3 | A heuristic algorithm for solving flexible job shop scheduling problem显示文摘 | Ziaee M | 2014 | International Journal of Advanced Manufacturing Technology2014，71，14： | 1 |
| 4 | Management of 10-15mm proximal ureteral stones:ureteroscopy or extracorporeal shockwave lithotripsy显示文摘 | Ziaee SA Halimiasl P Aminsharifi A | 2008 | J Urol2008，71，1： | 1 |
| 5 | Ultrasonographic versus fluoro- scopic access for pereutaneous nephrolithotomy a randomized clinical tri- al显示文摘 | Basifi A Ziaee AM Kianian HR | 2008 | J Endourol2008，22，2： | 1 |
| 6 | OCT4, an embryonic stem cell marker, is highly expressed in bladder cancer显示文摘 | Atlasi Y Mowla S J Ziaee S A | 2007 | Int J Cancer2007，120，7： | 1 |
| 7 | Ultrasonographic versus fluoroscopic access for percutaneous nephrolithotomy:a randomized clinical trial显示文摘 | Basiri A Ziaee AM Kianian HR | 2008 | J Endourol2008，22，2： | 1 |
| 8 | Management of 10-15ram proximal ureteral stones: ureteroscopy or extracorporeal shockwave lithotripsy?显示文摘 | Ziaee SA Halimiasl P Aminsharifi A | 2008 | Urology2008，71，1： | 1 |
| 9 | Ultrasonographic versus Fluor- oscopic Access for Percutaneous Nephrolithotomy : A Randomized Clin- ical Trial显示文摘 | Basiri A Ziaee AM Kianian HR | 2008 | J Endourol2008，22，2： | 1 |
| 10 | Totally ultrasonographyguided percutaneous nephrolithotomy in the flank position显示文摘 | Basiri A Ziaee SA Nasseh H | 2008 | J Endourol2008，22，： | 1 |
| 11 | Effects of Temperature on Cure Kinetics and Mechanical Properties of Vinyl-Ester Resins显示文摘 | PALMESE G R | 1999 | Journal of Polymer Science Part B:Polymer Physics1999，37，7： | 1 |
| 12 | OCT-4, an embryonic stem cell marker, is highly expressed in bladder cancer 显示文摘 | Aflasi Y J Mowla S Ziaee S A | 2007 | Int J Cancer2007，120，7： | 1 |
| 13 | Effect of low glycemic load diet on glycated hemoglobin (HbAtc) in poorly-controlled diabetes patients 显示文摘 | Ziaee A Afaghi A Sarreshtehdari M | 2011 | Glob J Health Sci2011，4，1： | 1 |
| 14 | OCT-4, an embryonic stem cell marker, is highly expressed in bladder cancer 显示文摘 | Atlasi Y Mowla S J Ziaee SA | 2007 | Int J Cancer2007，120，7： | 1 |
| 15 | Absence of PIWIL2 (HILI) expression in human bladder cancer cell lines and tissues 显示文摘 | Nikpour P Forouzandeh-Moghaddam M Ziaee S | 2009 | Cancer Epidemiol2009，33，34： | 1 |
| 16 | OCT-4, an embryonic stem cell marker, is highly expressed in bladder cancer 显示文摘 | Atlasi Y Mowla SJ Ziaee SA | 2007 | Int J Cancer2007，120，7： | 1 |
| 17 | Prediction of successful sperm retrieval in patients with nonobstructive azoospermia 显示文摘 | Ziaee SA Ezzatnegad M Nowroozi M | 2006 | Urol J2006，3，2： | 1 |
| 18 | Prevalence of hepatitis d virus infection among patients with chronic hepatitis B attending birjand hepatitis clinic(East of Iran) in 2012显示文摘 | Ziaee M Azarkar G | 2013 | Hepat Mon2013，13，11： | 1 |
| 19 | Pulmona~r function pa- rameters changes at different altitudes in healthy athletes 显示文摘 | Ziaee V Alizadeh R Movafegh A | 2008 | Iran J Allergy Asthma Immunol2008，7，： | 1 |
| 20 | OCT-4, an embryonic stemcell marker, is highly expressed in bladder cancer 显示文摘 | Atlasi Y Mowla SJ Ziaee SA | 2007 | Int J Canc- er2007，120，7： | 1 |
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