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| 1 | Prevalence of minimal hepatic encephalopathy and quality of life evaluations in hospitalized cirrhotic patients in China显示文摘AIM:To investigate the prevalence of minimal hepatic encephalopathy(MHE)and to assess corresponding health-related quality of life(HRQoL)in hospitalized cirrhotic patients in China.METHODS:This multi-center cross-sectional study included 16 teaching hospitals,which were members of 'Hepatobiliary Cooperation Group,Society of Gastroenterology,Chinese Medical Association',from different areas of China carried out between June and October in 2011.All the eligible hospitalized cirrhotic patients(n = 538)were required to complete triplicate number connection tests combined with one digit symbol test for diagnosing MHE.Patients' clinical examination data were complemented by a modified questionnaire assessing HRQoL.Written informed consent was obtained from each patient.RESULTS:Male was predominant(68.6%)in 519 patients who met the criteria of the study,with a mean age of 49.17 ± 11.02 years.The most common cause of liver cirrhosis was chronic hepatitis B(55.9%).The prevalence of MHE was 39.9% and varied by ChildPugh-Classification score(CPC-A:24.8%,CPC-B:39.4% and CPC-C:56.1%,P < 0.01).MHE(P < 0.01)and higher CPC scores(P < 0.01)were associated with a high HRQoL scores(reflecting poorer quality of life).The prevalence of MHE was proportionate to CPC(P = 0.01)and high quality of life scores(P = 0.01).CONCLUSION:Hospitalized cirrhotic patients have a high prevalence of MHE that is proportionate to the degree of liver function and HRQoL impairment. | Ji-Yao Wang Ning-Ping Zhang Bao-Rong Chi Yu-Qing Mi Li-Na Meng Ying-Di Liu Jiang-Bin Wang Hai-Xing Jiang Jin-Hui Yang Yun Xu Xiao Li Jian-Ming Xu Guo Zhang Xin-Min Zhou Yu-Zheng Zhuge De-An Tian Jin Ye Yu-Lan Liu | 2013 | World Journal of Gastroenterology2013，19，30： | 64 |
| 2 | Inhibition of long non-coding RNA TUG1 on gastric cancer cell transference and invasion through regulating and controlling the expression of miR-144/c-Met axis显示文摘Objective: To discuss the expression of long noncoding RNA TUG1(lnc RNA-TUG1) in gastric carcinoma(GC) and its effects on the transferring and invading capacity of gastric carcinoma cells. Methods: Forty cases of carcinoma tissue and para-carcinoma tissue were selected from GC patients who underwent surgical removal in Zhejiang Provincial Hospital of Chinese Traditional Medicine and Wenzhou Central Hospital from January, 2013 to December, 2014; the expressing level of lnc RNA-TUG1 in GC and para-C tissues was detected by applying the q RT-PCR technique. The correlation between lnc RNA-TUG1 expression and patients' clinical data was classified and analyzed. SGC-7901 cells were transfected using lnc RNA-TUG1 specific si RNA. Changes of the transferring and invading capacity of si RNAtransfected SGC-7901 cells were scratch-tested and transwell-detected. q RT-PCR was applied to detect the expression level of micro RNA-144 after lnc RNA-TUG1 was silenced. Changes of c-Met m RNA and protein expressions was detected by q RT-PCR and western-blot test. Results: The expression level of lnc RNA-TUG1 in GC tissue was significant higher than that in para-C tissue(P<0.05) and the high expression level of lnc RNA-TUG1 in GC tissue was significantly correlated with tumor lymph nodes metastasis and advance TNM phasing(P<0.05). The transferring and invading capacity of SGC-7901 cells was highly inhibited after being transfected by lnc RNA-TUG1 specific si RNA(P<0.05). The results of q RT-PCR and western-blot proved that the expression of micro RNA-144 was significantly boosted and the expression level of c-Met m RNA and protein was inhibited after lnc RNA-TUG1 was silenced(P<0.05). Conclusions: lnc RNA-TUG1 shows an up-regulated expression in GC tissue and that bears a correlation with clinicopathological features of malignant tumor. lnc RNATUG1 can promote the transferring and invading capacity of GC by inhibiting the pathway of micro RNA-144/c-Met. | Ting-Ting Ji Xuan Huang Jie Jin Sheng-Hua Pan Xiao-Ju Zhuge | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，5： | 20 |
| 3 | Preface显示文摘 | Yan-Bo Han Zhi-Wei Xu Hai Zhuge | 2006 | Journal of Computer Science & Technology2006，21，4： | 18 |
| 4 | Big-data analysis: A clinical pathway on endoscopic retrograde cholangiopancreatography for common bile duct stones显示文摘BACKGROUND A clinical pathway(CP)is a standardized approach for disease management.However,big data-based evidence is rarely involved in CP for related common bile duct(CBD)stones,let alone outcome comparisons before and after CP implementation.AIM To investigate the value of CP implementation in patients with CBD stones undergoing endoscopic retrograde cholangiopancreatography(ERCP).METHODS This retrospective study was conducted at Nanjing Drum Tower Hospital in patients with CBD stones undergoing ERCP from January 2007 to December 2017.The data and outcomes were compared by using univariate and multivariable regression/linear models between the patients who received conventional care(non-pathway group,n=467)and CP care(pathway group,n=2196).RESULTS At baseline,the main differences observed between the two groups were the percentage of patients with multiple stones(P<0.001)and incidence of cholangitis complication(P<0.05).The percentage of antibiotic use and complications in the CP group were significantly less than those in the nonpathway group[adjusted odds ratio(OR)=0.72,95%confidence interval(CI):0.55-0.93,P=0.012,adjusted OR=0.44,95%CI:0.33-0.59,P<0.001,respectively].Patients spent lower costs on hospitalization,operation,nursing,medication,and medical consumable materials(P<0.001 for all),and even experienced shorter length of hospital stay(LOHS)(P<0.001)after the CP implementation.No significant differences in clinical outcomes,readmission rate,or secondary surgery rate were presented between the patients in the non-pathway and CP groups.CONCLUSION Implementing a CP for patients with CBD stones is a safe mode to reduce the LOHS,hospital costs,antibiotic use,and complication rate. | Wei Zhang Bing-Yi Wang Xiao-Yan Du Wei-Wei Fang Han Wu Lei Wang Yu-Zheng Zhuge Xiao-Ping Zou | 2019 | World Journal of Gastroenterology2019，25，8： | 14 |
| 5 | Correlation of the occurrence of YMDD mutations with HBV genotypes,HBV-DNA levels,and HBeAg status in Chinese patients with chronic hepatitis B during lamivudine treatment显示文摘BACKGROUND:Continuous lamivudine therapy is associated with high rates of YMDD mutations,which are the main causes of drug resistance.The current study explores the association of the emergence of YMDD mutations with pretherapy HBV genotype,HBV-DNA levels,HBeAg status,and serum alanine aminotransferase(ALT) levels in Chinese patients receiving lamivudine therapy for chronic hepatitis B.METHODS:A total of 319 chronic hepatitis B patients who received lamivudine therapy for more than a year were enrolled in this study.YMDD mutations,HBV genotype,HBV-DNA levels,HBeAg status,and ALT levels were determined prior to their lamivudine treatment and every three months for a year of this therapy.RESULTS:Among the 319 patients,137(42.95%) were infected with genotype B and 182(57.05%) with genotype C.Up to 94 patients(29.47%) developed YMDD mutations within one year of lamivudine therapy.Furthermore,50 patients with HBV genotype B and 44 patients with genotype C developed YMDD mutations(36.50% vs 24.18%,P<0.05).Logistic regression analysis showed that pretherapy HBV genotype,HBV-DNA levels,and HBeAg status are independent factors for the emergence of YMDD mutations(HBV genotype:OR=2.159,95% CI 1.291-3.609,P=0.003;HBV-DNA:OR=1.653,95% CI 1.231-2.218,P=0.001;HBeAg:OR=2.021,95% CI 1.201-3.399,P=0.008).CONCLUSIONS:HBV genotype,HBV-DNA levels,and HBeAg status at baseline are the independent factors associated with the emergence of YMDD mutations among Chinese patients receiving lamivudine therapy for chronic hepatitis B.These findings are helpful to the development of therapeutic strategies for these patients. | Feng Wu,Min-Jin Wu,Xiao-Lin Zhuge,Shao-Ming Zhu and Biao Zhu Department of Chinese Pharmacy, Department of Laboratory Medicine , State Key Laboratory for Diagnosis and Treatment of Infectious Diseases , First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，2： | 11 |
| 6 | Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with proliferative diabetic retinopathy显示文摘·AIM: To determine the effects of laser photocoagulation on serum levels of angiopoietin-1(Ang-1),angiopoietin-2(Ang-2), soluble angiopoietin receptor Tie-2(Tie-2), Ang-1/Ang-2 ratio and vascular endothelial growth factor(VEGF) in patients with type 2diabetes mellitus(T2DM) and proliferative diabetic retinopathy(PDR). We also explored the role of the Ang/Tie system in PDR.·METHODS:Totally 160patientswithT2 DM, including50 patients with non-diabetic retinopathy(NDR), 58 patients with non-proliferative diabetic retinopathy(NPDR), and52 patients with PDR were enrolled in this study. Serum Ang-1, Ang-2, Tie-2 receptor and VEGF levels were measured using enzyme-linked immunosorbent assays for all patients and were repeated in 26 patients who underwent laser photocoagulation two months after the procedure.·RESULTS:ThemedianlevelsofAng-2andVEGFinserum were significantly higher in the NPDR group(4.23 ng/mL and 303.2 pg/mL, respectively) compared to the NDR group(2.67 ng/mL and 159.8 pg/mL, respectively, P <0.01), with the highest level in the PDR group(6.26 ng/mL and531.2 pg/mL, respectively, P <0.01). The median level of Ang-1 was significantly higher in the NPDR group(10.77ng/mL) compared to the NDR group(9.31 ng/mL) and the PDR groups(9.54 ng/mL)(P <0.05), while no difference was observed between the PDR and NDR groups. Ang-1/Ang-2 ratio of PDR group was lowest in three groups(1.49 vs 2.69 and 2.90, both P <0.01). The median level of Tie-2was not significantly different among three groups(P >0.05).Ang-2 was positively correlated with VEGF and Tie-2 in the PDR and NPDR groups(both P <0.05). Among the 26 patients who underwent laser photocoagulation, serum Ang-2 and VEGF levels significantly decreased(both P <0.05), whereas serum Ang-1 level and Ang-1/Ang-2ratio were weakly increased(P >0.05). The median levels of Ang-2 and VEGF in serum were highest in PDR group,however, Ang-1/Ang-2 ratio of PDR group was lowest in three groups.·CONCLUSION: Laser photocoagulation can reduce serum Ang-2 and VEGF levels. The Ang/Tie system and VEGF play an important role in the development and progression of T2 DM patients with PDR. | Qiao-Ying You Fu-Yuan Zhuge Qi-Qian Zhu Xu-Wei Si | 2014 | International Journal of Ophthalmology(English edition)2014，7，4： | 9 |
| 7 | Laboratory Evaluation of the Molluscicidal Activity of Pulsatilla Chinensis(Bunge)Regel Saponins against the Snail Oncomelania Hupensis显示文摘Objective To observe the toxicity of Pulsatilla chinensis(Bunge) Regel saponins(PRS) against Oncomelania hupensis(O.hupensis).Methods O.hupensis snails were exposed to 40% and 80% of 24 h LC50 of PRS for 24 h,and then choline esterase(CHE),alanine aminotransferase(ALT),alkaline phosphatase(ALP),lactate dehydrogenase(LDH) activities in cephalopodium and liver of snails were determined.Niclosamide(NIC) was used as the reference molluscicide.Zebra fish lethality test was evaluated to non-target aquatic species of PRS.Results The molluscicidal activity of PRS(LC50 at 24 h:0.48 mg/L) was similar to that of NIC(LC50 at 24 h:0.16 mg/L).Significant alterations about CHE,ALP,and ALT activities both in the cephalopodium and the liver of snails were observed when O.hupensis was exposed to 40% and 80% LC50 of PRS or NIC for 24 h.PRS and NIC could not affect LDH activity in the cephalopodium and the liver.Lower toxicity to fish of PRS was observed up to the highest concentration tested than NIC.Conclusion PRS,as compared with the reference molluscicide NIC,is thought to be used for the control of harmful vector snails safely. | CHEN Yan Qin XU Qiong Ming LIU Yan Li LI Xiao Ran YANG Shi Lin ZHUGE Hong Xiang | 2012 | Biomedical and Environmental Sciences2012，25，2： | 8 |
| 8 | Polyaspartic acid mediates the absorption and translocation of mineral elements in tomato seedlings under combined copper and cadmium stress显示文摘Polyaspartic acid(PASP)is a nontoxic,biodegradable,environmentally friendly polymer and is widely used as a fertilizer synergist in agricultural production.In many old orchards and vegetable gardens,highly fertile soil is often accompanied by severe heavy metal contamination.The present study was designed to investigate mineral element interactions mediated by PASP under copper(Cu)+cadmium(Cd)combined stress to provide reasonable suggestions for scientific fertilization.A pot experiment was conducted in which tomato seedlings served as plant materials.A concentration of 700 mgPASP and foliar spraying application methods were selected based on previous experiments.Four treatments were applied:normal soil(control(CK)),Cu+Cd(combined stress),Cu+Cd+PASP,and normal soil+PASP.The plant biomass,root activity,and mineral elements were measured,and these data were analyzed by Data Processing System(DPS)statistical software.The results showed that,under Cu+Cd combined stress,PASP promoted stem diameter growth,root activity and chlorophyll content and ultimately increased the biomass of tomato seedlings to different degrees.Moreover,the content of both Cu and Cd and their individual accumulation in plants decreased.PASP increased the distribution of Cu and Cd in the roots under Cu+Cd combined stress,and the translocation ability from the roots to shoots was significantly restricted.With respect to essential elements,PASP promoted mainly the absorption and translocation of potassium(K),calcium(Ca),and magnesium(Mg),which greatly exerted physiological roles.However,the variation trends of Cu and Cd under normal soil conditions contrasted with those under stress conditions.With respect to essential elements other than K,Ca,and Mg,PASP mostly restrained their absorption but promoted their translocation.The regulatory mechanism of PASP differed between the combined stress conditions and normal soil conditions.Under the combined stress conditions,PASP seemed to mainly promote these advantageous factors that exert physiological regulatory functions.Under normal soil conditions,PASP mainly acted as a biological stimulant or signaling molecule for increased nutrient efficiency,which caused greater biomass productivity. | HU Mei-mei DOU Qiao-hui CUI Xiu-min LOU Yan-hong ZHUGE Yu-ping | 2019 | Journal of Integrative Agriculture2019，18，5： | 8 |
| 9 | An integrated turbocharger design approach to improve engine performance显示文摘Turbocharging technology is today considered as a promising way for internal combustion engine energy saving and CO2 reduction.Turbocharger design is a major challenge for turbocharged engine performance improvement.The turbocharger designer must draw upon the information of engine operation conditions,and an appropriate link between the engine requirements and design features must be carefully developed to generate the most suitable design recommendation.The objective of this research is to develop a turbocharger design approach for better turbocharger matching to an internal combustion engine.The development of the approach is based on the concept of turbocharger design and interaction links between engine cycle requirements and design parameter values.A turbocharger through flow model is then used to generate the design alternatives.This integrated method has been applied with success to a gasoline engine turbocharger assembly. | ZHANG YangJun1,CHEN Tao1,ZHUGE WeiLin1,ZHANG ShuYong2 & XU JianZhong3 1 State Key Laboratory of Automotive Safety and Energy,Tsinghua University,Beijing 100084,China 2 National Key Laboratory of Diesel Engine Turbocharging Technology,P.O.B.22,Datong 037036,China 3 Institute of Engineering Thermophysics,Chinese Academy of Sciences,Beijing 100190,China | 2010 | Science China(Technological Sciences)2010，53，1： | 8 |
| 10 | Heterologous expression of human cytochrome P450 2E1 in HepG2 cell line显示文摘AIM: Human cytochrome P-450 2E1 (CYP2E1) takes part in the biotransformation of ethanol, acetone, many smallmolecule substrates and volatile anesthetics. CYP2E1 is involved in chemical activation of many carcinogens,procarcinogens, and toxicants. To assess the metabolic and toxicological characteristics of CYP2E1, we cloned CYP2E1 cDNA and established a HepG2 cell line stably expressing recombinant CYP 2E1.METHODS: Human CYP2E1 cDNA was amplified with reverse transcription-polymerase chain reaction (RT-PCR)from total RNAs extracted from human liver and cloned into pGEM-T vector. The cDNA segment was identified by DNA sequencing and subcloned into a mammalian expression vector pREP9. A transgenic cell line was established by transfecting the recombinant plasmid of pREP9-CYP2E1 to HepG2 cells. The expression of CYP2E1 mRNA was validated by RT-PCR. The enzyme activity of CYP2E1 catalyzing oxidation of 4-nitrophenol in postmitochondrial supernate (S9) fraction of the cells was determined by spectrophotometry. The metabolic activation of HepG2-CYP2E1 cells was assayed by N-nitrosodiethylamine (NDEA)cytotoxicity and micronucleus test.RESULTS: The cloned CYP2E1 cDNA segment was identical to that reported by Umeno et al(GenBank access No.J02843). HepG2-CYP2E1 cells expressed CYP2E1 mRNA and had 4-nitrophenol hydroxylase activity (0.162±0.025nmol.min-1.mg-1 S9 protein), which were undetectable in parent HepG2 cells. HepG2-CYP2E1 cells increased the cytotoxicity and micronucleus rate of NDEA in comparison with those of HepG2 cells.CONCLUSION: The cDNA of human CYP2E1 can be successfully cloned, and a cell line, HepG2-CYP2E1, which can efficiently express mRNA and has CYP2E1 activity, is established. The cell line is useful for testing the cytotoxicity,mutagenicity and metabolism of xenobiotics, which may possibly be activated or metabolized by CYP2E1. | Jian Zhuge Ye Luo Ying-Nian Yu Department of Pathophysiology,School of Medicine,Zhejiang University,Hangzhou 310031,Zhejiang Province,China | 2003 | World Journal of Gastroenterology2003，9，12： | 7 |
| 11 | Hepatic safety of caspofungin during treatment of invasive fungal diseases in elderly patients显示文摘To the Editor:Caspofungin is a new class of antifungal agents (echinocandins) for the treatment of fungal infections.By noncompetitive inhibition β-(1,3)-D-glucan synthase,it can destroy the glucan synthesis of the fungal cell wall and produce bactericidal activity.β-(1,3)-D-glucan does not exist in mammalian cells;thus the toxicity of this drug could be avoided. | WANG Li-li ZHUGE Xin XIAO Guang-hui ZHANG Yun | 2012 | Chinese Medical Journal2012，，12： | 6 |
| 12 | Hierarchical CuCo_2O_4@nickel-cobalt hydroxides core/shell nanoarchitectures for high-performance hybrid supercapacitors显示文摘Ni_(0.5)Co_(0.5)(OH)_2 nanosheets coated CuCo_2O_4 nanoneedles arrays were successfully designed and synthesized on carbon fabric. The core/shell nanoarchitectures directly served as the binder-free electrode with a superior capacity of 295.6 mAh g^(-1) at 1 Ag^(-1), which still maintained 220 mAh g^(-1) even at the high current density of 40 Ag^(-1), manifesting their enormous potential in hybrid supercapacitor devices. The asassembled CuCo_2O_4@Ni_(0.5)Co_(0.5)(OH)_2//AC hybrid supercapacitor device exhibited favorable properties with the specific capacitance as high as 90 Fg^(-1) at 1 Ag^(-1) and the high energy density of 32 Wh kg^(-1) at the power density of 800 Wkg^(-1). Furthermore, the as-assembled device also delivered excellent cycling performance(retaining 91.9% of the initial capacitance after 12,000 cycles at 8 Ag^(-1)) and robust mechanical stability and flexibility, implying the huge potential of present hierarchical electrodes in energy storage devices. | Chenggang Wang Kai Guo Weidong He Xiaolong Deng Peiyu Hou Fuwei Zhuge Xijin Xu Tianyou Zhai | 2017 | Science Bulletin2017，62，16： | 6 |
| 13 | The Relationship Between the Gut Microbiome and Neurodegen-erative Diseases显示文摘Many recent studies have shown that the gut microbiome plays important roles in human physiology and pathology.Also,microbiome-based therapies have been used to improve health status and treat diseases.In addition,aging and neurodegenerative diseases,including Alzheimer’s disease and Parkinson’s disease,have become topics of intense interest in biomedical research.Several researchers have explored the links between these topics to study the potential pathogenic or therapeutic effects of intestinal microbiota in disease.But the exact relationship between neurodegenerative diseases and gut microbiota remains unclear.As technology advances,new techniques for studying the microbiome will be developed and refined,and the relationship between diseases and gut microbiota will be revealed.This article summarizes the known interactions between the gut microbiome and neurodegenerative diseases,highlighting assay techniques for the gut microbiome,and we also discuss the potential therapeutic role of microbiome-based therapies in diseases. | Xueling Zhu Bo Li Pengcheng Lou Tingting Dai Yang Chen Aoxiang Zhuge Yin Yuan Lanjuan Li | 2021 | Neuroscience Bulletin2021，37，10： | 6 |
| 14 | Halftoning based algorithms for image hiding显示文摘 | Xia ZHUGE Koji Nakano | 2009 | 通讯和计算机(中英文版)2009，6，9： | 5 |
| 15 | 基于小波变换的变分辨率频谱的实现显示文摘将FFT与小波理论相结合,本文提出了用变频率分辨率频谱描述信号的思想,系统地介绍了该方法的原理,提出了实现算法.从仿真对比研究的结果看出,该方法比传统的信号分析方法更为优越。 | 沈国重 陈章位 James Zhuge 路甬祥 | 2000 | 声学学报2000，25，6： | 5 |
| 16 | Correlation of fibrinogen-like protein 2 with progression of acute pancreatitis in rats显示文摘AIM: To examine fibrinogen-like protein 2 (fgl2) expression during taurocholate-induced acute pancreatitis progression in rats and its correlation with pancreatic injury severity. METHODS: Forty-eight male Sprague-Dawley rats were randomly divided into the severe acute pancreatitis (SAP) group (n = 24) and the sham operation (SO) group (n = 24). Sodium taurocholate (4% at doses of 1 mL/kg body weight) was retrogradely injected into the biliopancreatic ducts of the rats to induce SAP. Pancreatic tissues were prepared immediately after sacrifice. At the time of sacrifice, blood was obtained for determination of serum amylase activity and isolation of peripheral blood mononuclear cells (PBMCs). Pancreatic tissue specimens were obtained for routine light microscopy including hematoxylin and eosin staining, and the severity of pancreatic injury was evaluated 1, 4 and 8 h after induction. Expression of fgl2 mRNA was measured in the pancreas and PBMCs using reverse transcription polymerase chain reaction. Expression of fgl2 protein was evaluated in pancreatic tissues using Western blotting and immunohistochemical staining. Masson staining was also performed to observe microthrombosis. RESULTS: At each time point, levels of fgl2 mRNAs in pancreatic tissues and PBMCs were higher (P < 0.05) in the SAP group than in the SO group. For pancreatic tissue in SAP vs SO, the levels were: after 1 h, 3.911 ± 1.277 vs 1.000 ± 0.673; after 4 h, 9.850 ± 3.095 vs 1.136 ± 0.609; and after 8 h, 12.870 ± 3.046 vs 1.177 ± 0.458. For PBMCs in SAP vs SO, the levels were: after 1 h, 2.678 ± 1.509 vs 1.000 ± 0.965; after 4 h, 6.922 ± 1.984 vs 1.051 ± 0.781; and after 8 h, 13.533 ± 6.575 vs 1.306 ± 1.179. Levels of fgl2 protein expression as determined by Western blotting and immunohistochemical staining were markedly up-regulated (P < 0.001) in the SAP group compared with those in the SO group. For Western blotting in SAP vs SO, the results were: after 1 h, 2.183 ± 0.115 vs 1.110 ± 0.158; after 4 h, 2.697 ± 0.090 vs 0.947 ± 0.361; and after 8 h, 3.258 ± 0.094 vs 1.208 ± 0.082. For immunohistochemical staining in SAP vs SO, the results were: after 1 h, 1.793 ± 0.463 vs 0.808 ± 0.252; after 4 h, 4.535 ± 0.550 vs 0.871 ± 0.318; and after 8 h, 6.071 ± 0.941 vs 1.020 ± 0.406. Moreover, we observed a positive correlation in the pancreas (r = 0.852, P < 0.001) and PBMCs (r = 0.735, P < 0.001) between fgl2 expression and the severity of pancreatic injury. Masson staining showed that microthrombosis (%) in rats with SAP was increased (P < 0.001) compared with that in the SO group and it was closely correlated with fgl2 expression in the pancreas (r = 0.842, P < 0.001). For Masson staining in SAP vs SO, the results were: after 1 h, 26.880 ± 9.031 vs 8.630 ± 3.739; after 4 h, 53.750 ± 19.039 vs 8.500 ± 4.472; and after 8 h, 80.250 ± 12.915 vs 10.630 ± 7.003.CONCLUSION: Microthrombosis due to fgl2 overexpression contributes to pancreatic impairment in rats with SAP, and fgl2 level may serve as a biomarker during early stages of disease. | Xiao-Hua Ye Tan-Zhou Chen Jia-Ping Huai Guang-Rong Lu Xiao-Ju Zhuge Ren-Pin Chen Wu-Jie Chen Chen Wang Zhi-Ming Huang | 2013 | World Journal of Gastroenterology2013，19，16： | 4 |
| 17 | Validation of the Nanjing Criteria for Diagnosing Pyrrolizidine Alkaloids-induced Hepatic Sinusoidal Obstruction Syndrome显示文摘Background and Aims:Hepatic sinusoidal obstruction syndrome(HSOS)is caused by toxic injury to sinusoidal endothelial cells in the liver.The intake of pyrrolizidine alkaloids(PAs)in some Chinese herbal remedies/plants remains the major etiology for HSOS in China.Recently,new diagnostic criteria for PA-induced HSOS(i.e.PA-HSOS)have been developed;however,the efficacy has not been clinically validated.This study aimed to assess the performance of the Nanjing criteria for PA-HSOS.Methods:Data obtained from consecutive patients in multiple hospitals,which included 86 PA-HSOS patients and 327 patients with other liver diseases,were retrospectively analyzed.Then,the diagnostic performance of the Nanjing criteria and simplified Nanjing criteria were evaluated and validated.The study is registered in www.chictr.org.cn(ID:ChiCTR1900020784).Results:The Nanjing criteria have a sensitivity and specificity of 95.35%and 100%,respectively,while the simplified Nanjing criteria have a sensitivity and specificity of 96.51%and 96.33%,respectively,for the diagnosis of PA-HSOS.Notably,a proportion of patients with Budd-Chiari syndrome(11/49)was misdiagnosed as PA-HSOS on the basis of the simplified Nanjing criteria,and this was mainly due to the overlapping features in the enhanced computed tomography/magnetic resonance imaging examinations.Furthermore,most of these patients(10/11)had occlusion or thrombosis of the hepatic vein,and communicating vessels in the liver were found in 8/11 patients,which were absent in PA-HSOS patients.Conclusions:The Nanjing criteria and simplified Nanjing criteria exhibit excellent performance in diagnosing PA-HSOS.Thus,both could be valuable diagnostic tools in clinical practice. | Wei Zhang Lu Liu Ming Zhang Feng Zhang Chunyan Peng Bin Zhang Jun Chen Lin Li Jian He Jiangqiang Xiao Yanhong Feng Xunjiang Wang Aizhen Xiong Li Yang Xiaoping Zou Yuecheng Yu Yuzheng Zhuge | 2021 | Journal of Clinical and Translational Hepatology2021，9，3： | 4 |
| 18 | Original article Activated changes of platelet ultra microstructure and plasma granule membrane protein 140 in patients with non-small cell lung cancer显示文摘背景非小的房间肺癌症(NSCLC ) 是世界范围的癌症死亡的领先的原因。血小板激活可以在肺癌症在病理学的进步起一个重要作用。在这研究,我们试图在肺癌症产生和生长上澄清激活的血小板的影响,并且在这些之中的关系功能并且血小板的 ultrastructural 变化和在有 NSCLC.Methods 的这些病人的病原的状况的严厉有病理地证实的 NSCLC 的病人的 136 个盒子在这研究被包括。54 个健康的人作为控制被注册。血血小板的极端微观结构和活动的变化在传播和扫描电子显微镜下面被观察。血浆小粒膜蛋白质 140 的同时的决心(GMP-140 ) 是 made.Results 传播电子显微镜学包括胀大,在有 NSCLC 的病人的血小板的极端微观结构的显示出的显著变化 o 小粒,泡,和 glycogenosome 增加。扫描电子显微镜学与 NSCLC 在病人在血小板上显示出许多更多的表面过程和皱纹。健康控制的 GMP-140 的参考血浆层次是(18.2 | ZHUGE Yi ZHOU Jian-ying YANG Guang-die ZU De-ling XU Xiao-liang TIAN Ming-qing LU Guo-hua | 2009 | Chinese Medical Journal2009，，9： | 4 |
| 19 | Utility of Droplet Digital PCR Assay for Quantitative Detection of Norovirus in Shellfish, from Production to Consumption in Guangxi, China显示文摘Objective Shellfish are recognized as important vehicles of norovirus-associated gastroenteritis. The present study aimed to monitor norovirus contamination in oysters along the farm-to-fork continuum in Guangxi, a major oyster production area in Southwestern China. Methods Oyster samples were collected monthly from farms, markets, and restaurants, from January to December 2016. Norovirus was detected and quantified by one-step reverse transcription-droplet digital polymerase chain reaction(RT-ddPCR). Results A total of 480 oyster samples were collected and tested for norovirus genogroups I and II. Norovirus was detected in 20.7% of samples, with genogroup II predominating. No significant difference was observed in norovirus prevalence among different sampling sites. The norovirus levels varied widely, with a geometric mean of 19,300 copies/g in digestive glands. Both norovirus prevalence and viral loads showed obvious seasonality, with a strong winter bias. Conclusion This study provides a systematic analysis of norovirus contamination ‘from the farm to the fork' in Guangxi. RT-ddPCR can be a useful tool for detection and quantification of low amounts of norovirus in the presence of inhibitors found particularly in foodstuffs. This approach will contribute to the development of strategies for controlling and reducing the risk of human illness resulting from shellfish consumption. | TAN Dong Mei LYU Su Ling LIU Wei ZENG Xian Ying LAN Lan QU Cong ZHUGE Shi Yang ZHONG Yan Xu XIE Yi Hong LI Xiu Gui | 2018 | Biomedical and Environmental Sciences2018，31，10： | 4 |
| 20 | Morphology and magnetic properties of Sm_(2)Co_(7)/α-Fe nanocomposite magnets produced by high energy ball milling and spark plasma sintering显示文摘In this article,the Sm_(2)Co_(7)/α-Fe nanocomposite magnets were prepared by high energy ball milling and spark plasma sintering method.The effect of soft phase content on the magnetic properties was studied.Up to 30 wt% α-Fe was added into Sm_(2)Co_(7) matrix without the decrease of remanence.Optimal energy product(BH)max of 9.2 MGOe was obtained with 20 wt% α-Fe.TEM observation shows that the grain size of α-Fe is 20-50 nm which ensures a good coupling effect between soft and hard phase.One more thing needs to be mentioned is that there exists inter-diffusion between Sm-Co phase and α-Fe phase.Moreover,our results can also illustrate that the Sm_(2)Co_(7)/α-Fe nanocomposite magnets are able to acquire better magnetic properties than the SmCo_(5)/α-Fe magnets prepared by the same process due to the large domain width of Sm_(2)Co_(7) phase. | Yitong Zhuge Yuqing Li Xiaochang Xu Dongtao Zhang Hongguo Zhang Weiqiang Liu Ming Yue | 2021 | Journal of Rare Earths2021，39，3： | 3 |
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