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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | USING DISPLACED CONE REPRESENTATION IN DEA MODELS FOR NONDOMINATED SOLUTIONSIN MULTIOBJECTIVE PROGRAMMING显示文摘Concepts revolving around 'displaced cones' are used to extend the ideas of nondominated solutions in multiobjective programming. Necessary and sufficient conditions are formed around the additive models of DEA to idelltify nondominated solutions. This provdes a basis for effecting contacts with other disciplines and other parts of DEA. | W. W. Cooper (Graduate School Of Business, University of Texas at Austin, Austin, Texas, USA 78712-1174)WEI Quanling (Department of Information Management, People’s University of China, Beliing 100872, China) G. Yu (Department of MSIS, University of Texas | 1997 | Systems Science and Mathematical Sciences1997，10，1： | 17 |
| 3 | Influence of whole peptidoglycan of bifidobacterium on cytotoxic effectors produced by mouse peritoneal macrophages显示文摘INTRODUCTIONBifidobacteria are physiologically beneficial bacteria which are perdominant in human intestine ,and possess the most important functions .They play an important role in maintaining microbial balance of the intestine .Furthermore , their presence is thought to be an important indication of health of the body [1-4].Whole peptidoglycan ( WPG) is the major component in the cell wall of bifidobacterium ,which is also a biological responsemodifier with nontoxic side dffcets. | Li Sheng Wang~1 Hui Ming Zhu~1 Dian Yuan Zhou~2 Yu Lin Wang~1 Wan Dai Zhang~2 ~1Departrnent of Gastroenterology,Shenzhen Municipal People’s Hospital,Jinan University of Medical Sciences,Shenzhen 518020,Guangdong Province,China ~2Chinese PLA Institute of Digestion,the First Military Medical University,Guangzhou 510515,Guangdong Province,ChinaLi Sheng Wang graduated and obtained Ph.D,from the First Military Medical University in 1998,now working at Department of Gastroenterology,Shenzhen Municipal People’s Hospital.Jinan University of Medical Sciences.having 35 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 15 |
| 4 | NEW SESQUITERPENES FROM LIGULARIA VEITCHIANA显示文摘Two new eremophilane derivatives and one new nor-sesquiterpene were isolated fromLigularia veitchiana.Their structures were elucidated by spectroscopic methods and 2D-NMR tech-niques and shown to be 1β,10β-epoxy-6β-(2′—semialdehyde acetal hydroxymethylacryloyloxy)-8β-ethoxy-eremophil-7(11)-en-12,8α-olide(1),1β-hydroxy-Δ~6,Δ~9-8-oxo-eremophil-(12)-oic-acid(2)and 1β-hydroxy-Δ~6,Δ~9-8-oxo-11-nor-11-hydroxy-eremophiladiene(3). | Yu ZHAO Zhong Jian JIA Institute of Organic Chemistry,State Key Laboratory of Applied Organic Chemistry at Lanzhou University,Lanzhou 730000. | 1993 | Chinese Chemical Letters1993，4，4： | 2 |
| 5 | TWO NEW OLEANOLIC ACID SAPONINS FROM ASTER YUNNANENSIS显示文摘TWONEWOLEANOLICACIDSAPONINSFROMASTERYUNNANENSIS￥YuSHAO;BingNanZHOU;LongZeLINandGeoffreyA.Cofdell(ShanghaiinstituteofMatstheMe... | Yu SHAO Bing Nan ZHOU Long Ze LIN and Geoffrey A. Cofdell (Shanghai institute of Matsthe Medica, Academic Sinica, Shanghai 2O0031 Program for Collaborative research in the Pharmaceutical Sciences, Coilege of pharmacy,Unlversity of lllinois at Chicago, I | 1994 | Chinese Chemical Letters1994，5，10： | 2 |
| 6 | An integrated model for the prediction of preeclampsia using maternal factors and uterine artery Doppler veloeimetry in unseleeted low-risk women 显示文摘 | Yu CK Smith GC Papageorghiou AT | 2005 | Am J Obstet Gynecol2005，193，2： | 1 |
| 7 | Fabrication of a no- vel core-shell gene delivery system based on a brush- like polycation of a, O-poly (L-Aspartate-Graft-PEI) 显示文摘 | Yu JH Quan JS Kwon JT et at | 2009 | Pharmaceutical Research2009，26，9： | 1 |
| 8 | Assessment of risk for the development of preaeclampsia by maternal characteristics and uterine artery Doppler 显示文摘 | PAPAGEORGHIOU AT YU CK ERASMUS IE | 2005 | BJOG2005，112，6： | 1 |
| 9 | An integrated model for the prediction of pre-eelampsia using maternal factors anduterine artery Doppler velocimetry in unselected low-risk women - American Journal of Obstetrics & Gyneeology显示文摘 | Yu CKH Smith GCS Papageorghiou AT | 2005 | American Journal of Obstetries & Gynecology2005，193，2： | 1 |
| 10 | Marker expre41on in circulating cancer cells of pancreatic cancer patients显示文摘 | Zhou J Hu L Yu Z at al | 2011 | J Surg Res2011，171，2： | 1 |
| 11 | Eficacy and safety of modified se-quential three-step empirical therapy for chronic cough 显示文摘 | Wei W Yu L Wang Y et at | 2010 | Re-spirology2010，15，5： | 1 |
| 12 | Wild-type P53 suppresses angiogenesis in human leiomyo-sarcoma andsynovial sarcoma by transcriptional suppression of vascular endothelial growth factor expression显示文摘 | Liangln Zhang Dihua Yu Mei Hu et at | 2000 | Cancer Res2000，60，13： | 1 |
| 13 | Fabrication and char- acterization of a flow-through PCR device with integrated chromium resistive heaters显示文摘 | Yu C Y Liang W S Kuan I et at | 2007 | Journal of the Chinese In- stitute of Chemical Engineers2007，38，： | 1 |
| 14 | Low-molecular-weight heparin for the treatment of acute ischemic stroke显示文摘 | Wong KS Yu YL Chan YW Tsoi TH Ahuja AT Chan FL Fong KY Law CB Wong A | 1995 | N Engl Med1995，333，： | 1 |
| 15 | Intravenous leiomyomatosis:two cases with different routes of tumor extension显示文摘 | LAM P M LO K W K YU M Y et at | 2004 | J Vasc Surg2004，39，： | 1 |
| 16 | Apelin and its receptor APJ in cardiovascular diseases 显示文摘 | YU X H TANG Z B LIU L J et at | 2014 | Clin Chim Acta2014，428，： | 1 |
| 17 | An overview of recent progress in the study of distributed multi-agent coordination 显示文摘 | Cao Y C Yu W W Ren W et at | 2013 | IEEE Transactions on Industrial Informatics2013，9，42： | 1 |
| 18 | The expression and significance of insulin- like growth factor-I receptor and its pathway on breast cancer stempro- genitors显示文摘 | Chang WW Lin R J Yu J et at | 2013 | Breast Cancer Res2013，15，3： | 1 |
| 19 | Hierarchical ensemble of global and local classifiers for face recognition显示文摘 | Su Yu Shan Shiguang Chen Xilin at el | 2009 | IEEE Transactions on Image Processing2009，18，8： | 1 |
| 20 | An integrated model for the prediction of pre-eclampsia using maternal factors and uterine artery Doppler velocimetry in unselected low-risk women 显示文摘 | Yu CK Smith GC Papageorghiou AT | 2006 | Am J Obstet Gynecol2006，195，1： | 1 |
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