| 1 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 2 | Binderless Carbon/Graphite Materials显示文摘High performance binderless carbon bodies can be mamufactured from the mesophase raw material derived from petroleum pitch, which is ground into fine powders less than 10μm, and directly moulded, then sintered at 1000℃ without any kind of binder. The sintered bodies have a bulk density of 1.69 g/cm3, bending strength of 91.2 MPa. Shore hardness of 100. Porosity of only 7.2%. and electrical resistivity of 60x10-6 m. The bodies graphitized at 2800℃ have a bulk density of 1.88 g/cm3, bending Strength of 82 MPa. Shore hardness of 85, porosityof 5.4%, and electrical resistivity Of 12x10-6 nm. The scanning electron micrographs of thesection show that the bodies possess an extremely dense and homogeneous structure. lnfra-redspectrum analysis shows that carbonyls introduced when ground or activated are one of the keyfactors determining the selfbinding and self sintering properties of the mesophase fine powders,and the Structure and strength of the sintered bodies. | Zhou, SM Xia, JT Yan, LC | 1997 | Journal of Materials Science & Technology1997，13，3： | 2 |
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