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| 1 | Magnetic resonance evaluation of transplanted mesenchymal stem cells after myocardial infarction in swine显示文摘Objectives To trace and evaluate intracoronary transplanted mesenchymal stem cells(MSCs) labeled with superparamagnetic iron oxide(SPIO) by using magnetic resonance imaging(MRI) in a swine model of myocardial infarction (MI).Methods MSCs were transfected with a lentiviral vector carrying the gene encoding green fluorescent protein (GFP) and labeled in vitro with SPIO.Two weeks after MI, swine were randomized to intracoronary transplantation of dual -labeled MSCs(n = 10),MSCs-GFP(n = 10) and saline(n = 5).MRI examination was performed with a 1.5T clinical scanner at 24 hours,3 weeks and 8 weeks after cells transplantation. Signal intensity(SI) changes,cardiac function and MI size were measured using MRI.Correlation between MR findings and histomorphologic findings was also investigated. Results The labeling efficiency at a combination of 25μg Fe/ml SPIO and 0.8 pi/ml Lipofectamine 2000 reached 100%.SPIO labeling did not affect GFP fluorescence and dual-labeling did not affect cell proliferation(P>0.05). Multipotentiality was not affected especially for cardiomyocyte-like cells differentiation.Cardiac cell marker of a-MHC and actinin were positively expressed by immunofluorescence staining after induction.SI on T2 * WI decreased substantial- ly in the interventricular septum 24 hours after injection of MSCs.The intensity of hypo-intense signals appeared to increase throughout the later time points.Changes in SI at 24 hours,3 weeks and 8 weeks were 52.98%±10.74%,21.53%±5.40%and 6.23%±2.01%,respectively(P<0.01).DE-MRI demonstrated both dual-labeled MSCSs and MSCs-GFP could dramatically reduce the size of MI and improve cardiac function. Histological data revealed that prussian blue stain-positive cells were found mainly in the border zone which also showed green fluorescence but negative for macrophage marker(CD68).Gross pathologic examination revealed that engrafted MSCs dramatically reduce the extent of necrotic myocardium and promote the regeneration of new,contractile myocardium along the subendocardial surface of the MI. Conclusions MSCs could be efficiently and safely labeled with SPIO and GFP,and could be detected reproducibly and noninvasively in vivo using cardiac MRI.Intracoronary transplantation of dual-labeled MSCs could increase cardiac function and reduce the size of MI. | KE Yang~1,PENG Chao-quan~1,XIANG Peng~1,ZHANG Cheng-xi~1, ZOU Li-yuan~1,WU Xiao~1,YA Gao~1,KANG Z huang~2,HE Ke-ke~2,LIU Jin-lai~1 (1.Department of Cardiology,The Third Affiliated Hospital, Sun Yat-sen University,Guangzhou 2.Department of Radiology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 5100630 3.Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University,Guangzhou 5100120) | 2011 | 岭南心血管病杂志2011，17，S1： | 7 |
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| 3 | Genetic association between IL-17Fgene polymorphisms and the pathogenesis of Graves'Disease in theHan Chinese population 显示文摘 | Ting G Ya H Wei Z | 2013 | Gene2013，512，2： | 1 |
| 4 | Transglutarninase treatment of pea proteins: effect on physicochemical and rheological properties of heat-induced protein gels显示文摘 | SHAND P J YA H PIETRASIK Z | 2008 | Food Chemistry2008，107，2： | 1 |
| 5 | Growth of ZnO nanorods by aqueous solution method with eleetrodeposited ZnO seed layers 显示文摘 | Liu Z F E L Ya J | 2009 | Appl Surf Sci2009，255，12： | 1 |
| 6 | Ginkgolides protect PC12 cells against hypoxia-induced injury by p42/p44 MAPK pathwaydependent upregulation of HIF-1 alpha expression and HIF-1DNA- binding activity显示文摘 | Li Z Ya K Mei WX | 2008 | J Cell Biechem2008，103，2： | 1 |
| 7 | Stress analyses and fatigue evaluation of rib-to-deck joints in steel orthotropic decks 显示文摘 | XIAO Z G YAMADA K YA S | 2008 | International Journal of Fatigue2008，30，80： | 1 |
| 8 | Estimates of the potential for energy conservation in the Chinese Steel Industry显示文摘 | BOQIANG L YA W LI Z | 2011 | Energy Policy2011，39，4： | 1 |
| 9 | Pulsed high - dose dexamethasone therapy in children with chronic idiopathic tbrombocytopenic purpura 显示文摘 | Wali YA A1 Lamki Z Shah W | 2002 | Pediatr Hematol Oncol2002，19，5： | 1 |
| 10 | 显示文摘 | ZHANG Z M YA()S LI YG el al | 2013 | ChemCommun2013，49，25： | 1 |
| 11 | Physicochemical and textural properties of heat- induced pea protein isolate gels 显示文摘 | P J Shand H Ya Z Pietrasik | 2007 | Food Chemistry2007，102，4： | 1 |
| 12 | Application of a Ferroelectric Plasma Cathode as a High-Current Switch显示文摘 | Krasik Ya E Chirko K Gleizer J Z | 2002 | The European Physical Journal D2002，，19： | 1 |
| 13 | Urotensin II contributes to the formation of lung adenocarcinoma inflammatory microenvironment through the NF -KB pathway in tumor-bearing nude mice显示文摘 | CHENG H Z YA Y W XIANG H C | 2012 | ONCOLOGY LEITERS2012，4，： | 1 |
| 14 | Transglutaminase treatment of pea proteins: Effect on physicochemical and rheological properties of heat-induced protein gels显示文摘 | SHAND P J YA H PIETRASIK Z | 2008 | Food Chem2008，107，2： | 1 |
| 15 | Physicochemical and textural properties of heat-induced pea protein isolate gels 显示文摘 | Shand P J Ya H Pietrasik Z | 2007 | Food Chemistry2007，102，4： | 1 |
| 16 | Stress Analyses and Fatigue Evaluation of Rib-to-deck Joints in Steel Orthotropic Decks显示文摘 | XIAO Z G YAMADA K YA S | | 0，，08： | 1 |
| 17 | Stress analyses and fatigue evaluation of rib-to-deck ioints in steel orth - otropie decks 显示文摘 | Xiao Z G Yamada K Ya S | 2008 | International Journal of Fatigue2008，30，8： | 1 |
| 18 | Mode structure in an anisotropic resonator consisting of two comer-cube prisms 显示文摘 | Vimik Ya Z Gcrasimov V B Krutin A Yu | 1990 | IEEE Journal of Quantum Electron1990，20，6： | 1 |
| 19 | Efficient production of recombi- nant IL-21 proteins for pre-clinical studies by a two-step dilution refolding method 显示文摘 | Chen Z Cui Y Leong YA | 2013 | lnt lmmunopharmacol2013，5769，13： | 1 |
| 20 | Ovarian cyst formation following GnRH agonist administration in IVF cycles:incidence and impact显示文摘 | Qublan HS Amarin Z Tahat YA | 2006 | Hum Reprod2006，21，： | 1 |
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