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| 1 | 查看详情显示文摘 | W C Huang H Y Tam P K A Wai X Y Dong H Ming J P Xie | | 中国物理快报(英文版)0，，： | 4 |
| 2 | Dopaminergic effects on in vitro osteogenesis显示文摘Multiple growth factors(e.g., BMP2, TGF-b1, FGF2) and isolated genes have been shown to improve osteoblastic proliferation and mineralization, advancing bone tissue engineering. Among these factors, both polydopamine(PDA) and dopamine(DA) monomer have recently been reported to increase osteoblast proliferation and mineralization in vitro. Although a well-characterized neurotransmitter, DA's role in the bone is unknown. We hypothesize that DA can directly act on osteoblasts, and examined whether osteoblasts express DA receptors that respond to exogenous DA. m RNAs and protein cell lysates were obtained from MC3T3-E1 cells during osteogenic differentiation phase. Reverse transcription polymerase chain reaction and western blot analysis were used to examine the expression of DA receptors, D1–D5. Dose-response effect and time course of DA treatment on cell proliferation, mineralization, and osteogenic differentiation were investigated at pre-determined days. Real-time PCR was performed to investigate whether DA affects osteogenic gene expression(ALP, BSP, OC, OSX, RUNX2, and Collagen1a2) with or without receptor antagonists(SCH233390 and GR103691). Two-way ANOVA was used for statistical analysis. All five DA receptors(D1, D2, D3, D4, and D5) m RNAs and proteins were expressed in MC3T3-E1 cells. DA treatment increased cell proliferation for up to 7 days(P, 0.05). Osteogenic mineralization was significantly greater in the DA-treated group than control group(P, 0.05). Finally, expression of all the osteogenic genes was inhibited by DA receptor antagonists for D1, D3, and D5. Our findings suggest that MC3T3-E1 osteoblasts express functional DA receptors that enhance proliferation and mineralization. PDA is not biologically inert and has important implications in orthopedic applications. Furthermore, osteoblast differentiation might be regulated by the nervous system, presumably during bone development, remodeling, or repair. | Dong Joon Lee Henry C Tseng Sing Wai Wong Zhengyan Wang Meng Deng Ching-Chang Ko | 2015 | Bone Research2015，3，3： | 3 |
| 3 | Novel role of STAT3 in microglia-dependent neuroinflammation after experimental subarachnoid haemorrhage显示文摘Background and purpose Signal transducer and activator of transcription 3(STAT3)may contribute to the proinflammation in the central nervous system diseases by modulating the microglial responses.Thus,this study was intended to investigate the effect of STAT3 on microglia-dependent neuroinflammation and functional outcome after experimental subarachnoid haemorrhage(SAH).Methods The SAH model was established by endovascular perforation in the mouse.Real-time PCR(RtPCR)and western blot were used to examine the dynamic STAT3 signalling pathway responses after SAH.To clarify the role of the STAT3 signalling pathway in the microglia-dependent neuroinflammation after SAH,the microglia-specific STAT3 knockout(KO)mice were generated by the Cre-LoxP system.The neurological functions were assessed by Catwalk and Morris water maze tests.Neuronal loss after SAH was determined by immunohistochemistry staining.Microglial polarisation status after STAT3 KO was then examined by RtPCR and immunofluorescence.Results The STAT3 and Janus kinase-signal transducer 2 activated immediately with the upregulation and phosphorylation after SAH.Downstream factors and related mediators altered dynamically and accordingly.Microglial STAT3 deletion ameliorated the neurological impairment and alleviated the early neuronal loss after SAH.To investigate the underlying mechanism,we examined the microglial reaction after STAT3 KO.STAT3 deletion reversed the increase of microglia after SAH.Loss of STAT3 triggered the early morphological changes of microglia and primed microglia from M1 to M2 polarisation.Functionally,microglial STAT3 deletion suppressed the SAH-induced proinflammation and promoted the anti-inflammation in the early phase.Conclusions STAT3 is closely related to the microglial polarisation transition and modulation of microglia-dependent neuroinflammation.Microglial STAT3 deletion improved neurological function and neuronal survival probably through promoting M2 polarisation and anti-inflammatory responses after SAH.STAT3 may serve as a promising therapeutic target to alleviate early brain injury after SAH. | Zhiyuan Vera Zheng Junfan Chen Hao Lyu Sin Yu Erica Lam Gang Lu Wai Yee Chan George K C Wong | 2022 | Stroke & Vascular Neurology2022，7，1： | 3 |
| 4 | Tracking control based on neural network strategy for robot manipulator 显示文摘 | Wai R J Chang C J | 2003 | Neurocomputing2003，51，： | 1 |
| 5 | Microemulsion templated synthesis of carbon nanotube-supported Pd and Rh nanoparticles for ca- talytic applications显示文摘 | Yoon B Wai C M | 2005 | J Am Chem Soc2005，127，17： | 1 |
| 6 | Self-starting of passively mode-locked lasers with fast saturable absorbers显示文摘 | Chen C J Wai P K Menyuk C R | 1995 | Optics Letter1995，20，： | 1 |
| 7 | Further validation of the Chinese version of the level of expressed emotion scale for re search and clinical use显示文摘 | Wai - Tong Chiena Sally W Chan C | 2010 | International Journal of Nursing Stud- ies2010，47，： | 1 |
| 8 | Synthesizing and dispersing silver nanoparticles in a water-in supercritical carbon dioxide microemulsion显示文摘 | Chen X Y Wai C M | 1999 | J Am Chem Soc1999，121，： | 1 |
| 9 | 显示文摘 | Chen X Y Wai C M | 1999 | J Am Chem Soc1999，121，： | 1 |
| 10 | Osteopontin regulation in tumor metastasis 显示文摘 | Wai P Y Kuo P C | 2008 | Cancer Metastasis Rev2008，27，1： | 1 |
| 11 | Novel maximum-power- extraction algorithm for PMSG wind generations显示文摘 | Wai R J Lin C Y Chang Y R | 2007 | lET Electric PowerApplications2007，1，2： | 1 |
| 12 | Osteopontin inhibits macrophage nitric oxide synthesis to enhance tumor proliferation显示文摘 | Wai PY Guo L Gao C | | 0，，02： | 1 |
| 13 | Supercritical fluid extraction in herbal and natural product studies-a practical review显示文摘 | LANG Q Y WAI C M | 2001 | Talanta2001，53，： | 1 |
| 14 | Tracking control based on neural network strategy for robot nmnipulator 显示文摘 | Wai R J Chang C J | 2003 | Neurocomputing2003，51，7： | 1 |
| 15 | Bioelectrochemical immunoassay of polychlorinated biphenyl显示文摘 | Lin Y Y Liu G D Wai C M | 2008 | Analytica Chimica Acta2008，612，： | 1 |
| 16 | Synthesizing and dispersing silver nanoparticles in a water-in-supercritical carbon dioxide microemulsion显示文摘 | JI M CHEN X Y WAI C M | 1999 | J Am Chem Soc1999，121，： | 1 |
| 17 | The role of osteopontin in tumor metastasis显示文摘 | Wai P Y Kuo P C | 2004 | J Surg Res2004，121，2： | 1 |
| 18 | A novel technique for high impedance fault identification 显示文摘 | Wai D C T Xia Y B | 1998 | IEEE Transactions on Power Delivery1998，13，3： | 1 |
| 19 | Design of Voltage Tracking Control forDC-DC Boost Converter Via Total Sliding-Mode Technique显示文摘 | Wai R J Shih L C | 2011 | Transactions on industrial electronics2011，58，6： | 1 |
| 20 | Polarization mode dispersion,decorrelation,and diffusion in optical fibers with randomly varying birefringence显示文摘 | P K A Wai C R Menyuk | 1996 | J Lightwave Technol1996，14，2： | 1 |
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