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| 1 | Identification and characterization of genes that control fat deposition in chickens显示文摘Background: Fat deposits in chickens contribute significantly to meat quality attributes such as juiciness, flavor,taste and other organoleptic properties. The quantity of fat deposited increases faster and earlier in the fastgrowing chickens than in slow-growing chickens. In this study, Affymetrix Genechip? Chicken Genome Arrays 32773transcripts were used to compare gene expression profiles in liver and hypothalamus tissues of fast-growing and slow-growing chicken at 8 wk of age. Real-time RT-PCR was used to validate the differential expression of genes selected from the microarray analysis. The mRNA expression of the genes was further examined in fat tissues. The association of single nucleotide polymorphisms of four lipid-related genes with fat traits was examined in a F2resource population.Results: Four hundred genes in the liver tissues and 220 genes hypothalamus tissues, respectively, were identified to be differentially expressed in fast-growing chickens and slow-growing chickens. Expression levels of genes for lipid metabolism(SULT1B1, ACSBG2, PNPLA3, LPL, AOAH) carbohydrate metabolism(MGAT4B, XYLB, GBE1, PGM1,HKDC1)cholesttrol biosynthesis(FDPS, LSS, HMGCR, NSDHL, DHCR24, IDI1, ME1) HSD17B7 and other reaction or processes(CYP1A4, CYP1A1, AKR1B1, CYP4V2, DDO) were higher in the fast-growing White Recessive Rock chickens than in the slow-growing Xinghua chickens. On the other hand, expression levels of genes associated with multicellular organism development, immune response, DNA integration, melanin biosynthetic process, muscle organ development and oxidation-reduction(FRZB, DMD, FUT8, CYP2C45, DHRSX, and CYP2C18) and with glycol-metabolism(GCNT2, ELOVL 6, and FASN), were higher in the XH chickens than in the fast-growing chickens. RT-PCR validated high expression levels of nine out of 12 genes in fat tissues. The G1257069A and T1247123C of the ACSBG2 gene were significantly associated with abdominal fat weight. The G4928024A of the FASN gene were significantly associated with fat bandwidth, and abdominal fat percentage. The C4930169T of the FASN gene was associated with abdominal fat weight while the A59539099G of the ELOVL 6 was significantly associated with subcutaneous fat. The A8378815G of the DDT was associated with fat band width.Conclusion: The differences in fat deposition were reflected with differential gene expressions in fast and slow growing chickens. | Hirwa Claire D'Andre Wallace Paul Xu Shen Xinzheng Jia Rong Zhang Liang Sun Xiquan Zhang | 2014 | Journal of Animal Science and Biotechnology2014，5，1： | 7 |
| 2 | Using quality improvement methods to increase use of pain prevention strategies for childhood vaccination显示文摘AIM To increase evidence-based pain prevention strategy use during routine vaccinations in a pediatric primary care clinic using quality improvement methodology.METHODS Specific intervention strategies(i.e.,comfort positioning,nonnutritive sucking and sucrose analgesia,distraction) were identified,selected and introduced in three waves,using a Plan-Do-Study-Act framework.System-wide change was measured from baseline to post-intervention by:(1) percent of vaccination visits during which an evidence-based pain prevention strategy was reported as being used; and(2) caregiver satisfaction ratings following the visit.Additionally,self-reported staff and caregiver attitudes and beliefs about pain prevention were measured at baseline and 1-year post-intervention to assess for possible long-term cultural shifts.RESULTS Significant improvements were noted post-intervention.Use of at least one pain prevention strategy was documented at 99% of patient visits and 94% of caregivers were satisfied or very satisfied with the pain prevention care received.Parents/caregivers reported greater satisfaction with the specific pain prevention strategy used [t(143) = 2.50,P ≤ 0.05],as well as greater agreement that the pain prevention strategies used helped their children's pain [t(180) = 2.17,P ≤ 0.05] and that they would be willing to use the same strategy again in the future [t(179) = 3.26,P ≤ 0.001] as compared to baseline.Staff and caregivers also demonstrated a shift in attitudes from baseline to 1-year post-intervention.Specifically,staff reported greater agreement that the pain felt from vaccinations can result in harmful effects [2.47 vs 3.10; t(70) =-2.11,P ≤ 0.05],less agreement that pain from vaccinations is 'just part of the process' [3.94 vs 3.23; t(70) = 2.61,P ≤ 0.05],and less agreement that parents expect their children to experience pain during vaccinations [4.81 vs 4.38; t(69) = 2.24,P ≤ 0.05].Parents/caregivers reported more favorable attitudes about pain prevention strategies for vaccinations across a variety of areas,including safety,cost,time,and effectiveness,as well as less concern about the pain their children experience with vaccination [4.08 vs 3.26; t(557) = 6.38,P ≤ 0.001],less need for additional pain prevention strategies [3.33 vs 2.81; t(476) = 4.51,P ≤ 0.001],and greater agreement that their doctors' office currently offers pain prevention for vaccinations [3.40 vs 3.75; t(433) =-2.39,P ≤ 0.05].CONCLUSION Quality improvement methodology can be used to help close the gap in implementing pain prevention strategies during routine vaccination procedures for children. | Jennifer Verrill Schurman Amanda D Deacy Rebecca J Johnson Jolynn Parker Kristi Williams Dustin Wallace Mark Connelly Lynn Anson Kevin Mroczka | 2017 | World Journal of Clinical Pediatrics2017，6，1： | 3 |
| 3 | Monodisk: device for percutaneous transcather closure of cardiac septal defects显示文摘 | Pavcnik D WrigKC Wallace S | 1993 | Cardiovasc Intervent Radiol1993，16，5： | 2 |
| 4 | 显示文摘 | Wilk G D Wallace R M Anthony J M | 2001 | Journal of Applied Physics2001，89，10： | 2 |
| 5 | Synthesis and antimitotic/cytotoxic activity of hemiasterlin analogues 显示文摘 | Nieman J A Coleman J E Wallace D J | 2003 | J Nat Prod2003，66，： | 1 |
| 6 | The international at-sea intercomparison of f CO2 systems during the R/V Meteor cruise 36/1 in the North Atlantic Ocean 显示文摘 | Kortzinger A Mintrop L Wallace D W R | 2000 | Marine Chemistry2000，72，24： | 1 |
| 7 | Mitochondrial DNA in aging and disease显示文摘 | WALLACE D C | 1997 | Sci Am1997，277，： | 1 |
| 8 | Nosocomial bloodstream infections in United States hospitals: a three-year analysis显示文摘 | EDMOND M B WALLACE S E MCCLISH D K | 1999 | Clin Infect1999，29，23： | 1 |
| 9 | Regional climate impacts of the Northern Hemisphere annular mode显示文摘 | Thompson D W J Wallace J M | 2001 | Science2001，29，3： | 1 |
| 10 | The cellular and molecular basis of gastric mucosal defense显示文摘 | WALLACE J L GRANGER D N | 1996 | FASEB J1996，10，7： | 1 |
| 11 | Improved high-contrast imaging with on-axis telescopes using a multistage vortex coronagraph 显示文摘 | Mawet D Serabyn E Wallace J K | 2011 | Optics Letters2011，36，8： | 1 |
| 12 | Functional selectivity for glycerol of the nodulin 26 subfamily of plant membrane intrinsic proteins显示文摘 | Wallace I S Wills D M Guenther J F | 2002 | FEBS Lett2002，523，： | 1 |
| 13 | A randomized,controlled pilot study of a home-based exercise program for individuals with mild and moderate stroke显示文摘 | Duncan PW Richards L Wallace D | 1998 | Stroke1998，29，10： | 1 |
| 14 | Reduced B lymphocyte and immunoglobulin levels after atacicept treatment in patients with systemic lupus erythematosus:results of a multicenter,phase Ib,double-blind,placebo-controlled,dose-escalating trial显示文摘 | Dall'Era M Chakravarty E Wallace D | 2007 | Arthritis Rheum2007，56，： | 1 |
| 15 | Prolonged exposure of naive CD8+T cells to interleukin-7 or interleukin-15 stimulates proliferation without differentiation or loss of telomere length 显示文摘 | Wallace D L B~rard M Soares M V | 2006 | Immunology2006，119，2： | 1 |
| 16 | Specific allergy immunotherapy for allergic rhini- tis:subcutaneous and sublingual 显示文摘 | Cox L Wallace D | 2011 | Immunol Allergy Clin North Am2011，31，3： | 1 |
| 17 | Value of C-reactive protein measurements in exacerbations of chronic obstructive pulmonary disease显示文摘 | Dev D Wallace E Sankaran R | 1998 | Respir Med1998，92，4： | 1 |
| 18 | Titanium Alloys and Processing for High Speed Aircraft显示文摘 | Brewer D Bird R K Wallace T A | 1998 | Materials Science and Engineering1998，243，： | 1 |
| 19 | Constitutive expression ofafruitphytoene synthase gene in transgenic tomatoes cause dwarfism by redirecting metabolites from the gibberellin pathway 显示文摘 | Fray R G Wallace A Fraser P D | 1995 | The Plant Journal1995，，8： | 1 |
| 20 | Planetary-scale atmospheric phenomena associated with the Southern Oscillation显示文摘 | HOREL J D WALLACE J M | 1981 | Mon Wea Rev1981，109，： | 1 |
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