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| 1 | miR-20b, miR-98, miR-125b-1*, and let-7e* as new potential diagnostic biomarkers in ulcerative colitis显示文摘AIM:To use microarray-based miRNA profiling of colonic mucosal biopsies from patients with ulcerative colitis (UC), Crohn's disease (CD), and controls in order to identify new potential miRNA biomarkers in inflammatory bowel disease. METHODS:Colonic mucosal pinch biopsies from the descending part were obtained endoscopically from patients with active UC or CD, quiescent UC or CD, as well as healthy controls. Total RNA was isolated and miRNA expression assessed using the miRNA microarray Geniom Biochip miRNA Homo sapiens (Febit GmbH, Heidelberg, Germany). Data analysis was carried out by principal component analysis and projection to latent structure-discriminant analysis using the SIMCA-P+12 software package (Umetrics, Umea, Sweden). The microarray data were subsequently validated by quantitative real-time polymerase chain reaction (qPCR) performed on colonic tissue samples from active UC patients (n = 20), patients with quiescent UC (n = 19), and healthy controls (n = 20). The qPCR results were analyzed with Mann-WhitneyU test.In silico prediction analysis were performed to identify potential miRNA target genes and the predicted miRNA targets were then compared with all UC associated susceptibility genes reported in the literature. RESULTS:The colonic mucosal miRNA transcriptome differs significantly between UC and controls, UC and CD, as well as between UC patients with mucosal inflammation and those without. However, no clear differences in the transcriptome of patients with CD and controls were found. The miRNAs with the strongest differential power were identified (miR-20b, miR-99a, miR-203, miR-26b, and miR-98) and found to be upregulated more than a 10-fold in active UC as compared to quiescent UC, CD, and controls. Two miRNAs, miR-125b-1* and let-7e*, were up-regulated more than 5-fold in quiescent UC compared to active UC, CD, and controls. Four of the seven miRNAs (miR-20b, miR-98, miR-125b-1*, and let-7e*) were validated by qPCR and found to be specifically upregulated in patients with UC. Usingin silico analysis we found several predicted pro-inflammatory target genes involved in various pathways, such as mitogen-activated protein kinase and cytokine signaling, which are both key signaling pathways in UC.CONCLUSION:The present study provides the first evidence that miR-20b, miR-98, miR-125b-1*, and let7e* are deregulated in patients with UC. The level of these miRNAs may serve as new potential biomarkers for this chronic disease. | Mehmet Coskun Jacob Tveiten Bjerrum Jakob Benedict Seidelin Jesper Thorvald Troelsen JΦrgen Olsen Ole Haagen Nielsen | 2013 | World Journal of Gastroenterology2013，19，27： | 19 |
| 2 | Does ethnicity and education influence preoperative disability and expectations in patients undergoing total knee arthroplasty?显示文摘AIM To investigate whether minority ethnicity and the duration of education influence preoperative disability and expectations in patients undergoing total knee arthroplasty.METHODS We prospectively included 829 patients undergoing primary unilateral total knee arthroplasty(TKA) from April 2013 to December 2014 at a single centre. Patients filled in pre-operative questionnaires with information regarding place of birth, duration of education, expectations for outcome of surgery and baseline characteristics. Patients were stratified based on ethnicity. Majority ethnicity was defined as born inthe study country and minority ethnicity was defined as born in any other country. Similarly, patients were stratified based on duration of education in groups defined as < 9 years, 9-12 years and > 12 years, respectively.RESULTS We found that 92.2% of patients were of majority ethnicity. We found that 24.5%, 44.8% and 30.8% of patients had an education of < 9 years, 9-12 years and > 12 years, respectively. The mean preoperative(preOP) oxford knee score(OKS) in the total population was 23.6. Patients of minority ethnicity had lower mean pre-OP OKS(18.6 vs 23.9, P < 0.001), higher pain levels(VAS 73.0 vs 58.7, P < 0.001), expected higher levels of post-OP pain(VAS 14.1 vs 6.1, P = 0.02) and of overall symptoms(VAS 16.6 vs 6.4, P = 0.006). Patients with > 12 years education had lower mean pre-OP OKS(21.5 vs 23.8 and 24.6, P < 0.001) and higher pre-OP VAS pain(65.4 vs 59.2 and 56.4, P < 0.001) compared to groups with shorter education. One year post-operative(post-OP) patients of minority ethnicity had lower mean OKS, higher pain and lower QoL. One year post-OP patients with > 12 years education reported higher pain compared to patients with shorter educations. However, the response-rate was low(44.6%), and therefore post-OP results were not considered to be significant.CONCLUSION Minority ethnicity and the duration of education influ-ence preoperative disability and expectation in patients undergoing TKA. This should be taken into account when patients are advised pre-operatively. | Madeline Therese Kudibal Thomas Kallemose Anders Troelsen Henrik Husted Kirill Gromov | 2018 | World Journal of Orthopedics2018，9，10： | 3 |
| 3 | 髋关节畸形的发生率及其与性别、腹股沟痛和骨关节炎发病风险的关系——一项人口调查研究显示文摘背景:关于股骨髋臼撞击症的临床结果已有较多阐述,但对髋关节解剖畸形在一般人群中的发生率、自然病史和骨关节炎发生风险评估的研究甚少。方法:此抽样调查资料来源于1991至1994年哥本哈根骨关节炎子研究的4151例人口调查影像学及问卷调查队列研究数据。调查对象主要为丹麦哥本哈根sterbro地区的白人。符合纳入标准的男性1332名,女性2288名。根据影像学标准,髋关节被分为无畸形或有畸形,畸形中包括臼窝过深、枪柄样畸形和臼窝过深合并枪柄样畸形。髋关节骨关节炎影像学上定义为最小关节间隙宽度≤21mm。结果:3620名调查对象中髓臼发育不良的发生率为男4.3%,女3.6%:臼窝过深为男15.2%,女19.4%;枪柄样畸形为男19.6%,女5.2%;臼窝过深合并枪柄样畸形为男2.9%,女0.9%。正常髋臼顶比例为男80.7%,女77.0%。我们发现影像学上显示有髋关节畸形的调查对象其腹股沟痛的发生率无显著增加(P均〉0.13)。臼窝过深是骨关节炎发生的一个显著危险因素(风险比为2.4),枪柄样畸形亦为显著危险因素(风险比为2.2)。髋臼发育不良及性别不是髋关节骨关节炎发生的显著危险因素(P值分别为0.053和0.063)。靛关节骨关节炎发生率为男9.5%,女11.2%。髋关节骨关节炎并发畸形的发生率为男71.o%,女36.6%。结论:本组调查对象中,臼窝过深及枪柄样畸形是比较常见的影像学改变,与髋关节骨关节炎的发生风险增加相关。与髋关节畸形相关的骨关节炎高发生率提示我们应该更多地关注髋关节的早期畸形。 | Kasper Kjaerlf Gosvig Steffen Jacobsen Sfig Sonne-Holm Henrik Palm Anders Troelsen 付勤(译) | 2010 | 中华骨科杂志2010，30，12： | 2 |
| 4 | Transcriptional regulation of pigactase-phlorizin hydroase:involvement of HNF-1 and FREACs显示文摘 | Spodsberg N Troelsen JT Carlsson P | 1999 | Gastroenterology1999，116，4： | 1 |
| 5 | Transcriptional Regulation of pig Laetase- phlorizin Hydrolase involvement of HNF- 1 and FREACs 显示文摘 | Spodsberg N Troelsen J T Carlsson P | 1999 | Gastroenterology1999，116，： | 1 |
| 6 | The HOXCll Ho- meodomain Protein Interacts with the Lactase- Phlorizin Hydrolase Promoter and Stimulates HNF1-dependent Transcription 显示文摘 | Mitchelmore C Troelsen J T Sjostrom H | 1998 | Bio- ehem1998，273，13： | 1 |
| 7 | lkb of the lactase-phlorizin hydrolase promoter directes post-weaning decline and small intestinal- specific expression in transgenic mice 显示文摘 | TROELSEN J T MEHLUM A OLSEN J | 1994 | FEBS Letters1994，342，3： | 1 |
| 8 | Two intesinal specific nuclear factors binding to the lactase phlorizin hydrolase and sucrase-isomaltase promoter are functionally related oligomeric molecules显示文摘 | Troelsen JT Olsen J Mitchelmore C | 1994 | FEBS Lett1994，342，3： | 1 |
| 9 | The role of CDX2 in intestinal homeostasis and inflammation 显示文摘 | Coskun M Troelsen JT Nielsen OH | 2011 | Biochim Biophys Aeta2011，1812，3： | 1 |
| 10 | A novel intestinal trans-fac- tor (NF-LPH1) interacts with the lactase-phlorizin hydrolase pro- moter and co-varies with the enzymatic activity 显示文摘 | Troelsen J T Olsen J Noren 0 | 1992 | Biological Chemistry1992，267，20： | 1 |
| 11 | Transcriptional regula- tion of pig lactase-phlorizin hydrolase involvement of HNF-1 and FREACs 显示文摘 | SPODSBERG N TROELSEN J T CARLSSON P | 1999 | Gastroenterology1999，116，： | 1 |
| 12 | What is the role of clinical tests and ultrasound in acetabular labral tear diagnostics?显示文摘 | Troelsen A Mechlenburg I Gelineck J | 2009 | Acta Orthop2009，80，3： | 1 |
| 13 | Weight bearing anteroposterior pelvic radiographs are recommended in DDH assessment显示文摘 | Troelsen A Jacobsen S Romer L | 2008 | Clin Orthop Relat Res2008，4，5： | 1 |
| 14 | A new minimally invasive transsartorial approach for periacetabular osteotomy显示文摘 | TROELSEN A ELMENGAARD B SOBALLE K | 2008 | J Bone Joint Surg Am2008，90，3： | 1 |
| 15 | Weightbearing anteroposterior pelvic radiographsare recommended in DPH assessment 显示文摘 | Troelsen A Jacobson S Remer L | 2008 | Clin Orthop2008，466，： | 1 |
| 16 | Prevalence and mortal ity of acute myocardial infarction in patients with diabetes 显示文摘 | Rytter L Troelsen S Beck-Nielsen H | 1985 | Diabetes Care1985，8，3： | 1 |
| 17 | Blue eye color in humans may be caused by a perfectly associated founder mutation in a regulatory element located within the HERC2 gene inhibiting OCA2 expression 显示文摘 | Eiberg H Troelsen J Nielsen M | 2008 | Hum Genet2008，123，2： | 1 |
| 18 | Transcriptional regulation of pig lactase-phlorizin hydrolase:involvement of HNF-1 and FREACs 显示文摘 | Spodsberg N Troelsen JT Carlsson P | 1999 | Gastroenterology1999，116，4： | 1 |
| 19 | Serotonin transporter density and anxiolytic-like effects of antidepressants in mice显示文摘 | Mirza NR Nielsen EO Troelsen KB | 2007 | Prog Neuropsychopharmacol Biol Psychiatry2007，31，4： | 1 |
| 20 | Comparison of the min- imally invasive and ilioinguina显示文摘 | Troelsen A Elmengaard B Soballe K | 2008 | Acta Orthop2008，79，6： | 1 |
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