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| 1 | Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells显示文摘To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesen-chymal stem cells (BMSCs) were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissue- engineered skin. The results showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle shape to round or oval; tonofibrils, melano-somes and keratohyaline granules were observed under a transmission electronic microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% ± 4.2%) and epidermal cells differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06 pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material short-ened skin wound repair significantly. In one word, autogenic BMSCs have the potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering. | HE LiJuan, NAN Xue, WANG YunFang, GUAN LiDong, BAI CiXian, SHI ShuangShuang, YUAN HongFeng, CHEN Lin, LIU DaQing & PEI XueTao Laboratory of Stem Cells and Regenerative Medicine, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China | 2007 | Science China(Life Sciences)2007，50，4： | 16 |
| 2 | Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye显示文摘Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bi-polar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Elec-troretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas. | ZHANG Jie1,2, SHAN Qing1, MA Ping1, JIANG Yanming1, CHEN Peng1, WEN Jingxia1, ZHOU You1, QIAN Huanwen1 & PEI Xuetao2 1. Beijing Institute of Radiation Medicine, Beijing 100850, China 2. Beijing Institute of Transfusion Medicine, Beijing 100850, China | 2004 | Science China(Life Sciences)2004，47，3： | 10 |
| 3 | On the“plasticity”of adult stem cells and its application in regenerative medicine显示文摘In recent years, with the increasingly further studies on embryonic stem cells and the recognition of the biologic characteristics of adult stem cells, it has been discovered that adult stem cells have another phenomenon of 'Plasticity' in addition to the characteristics of strong poten- tial for self-renewal, proliferation and multi-differentiation, which brings us the hope for regenerative medicine —— renewing new organ or tissue cells to replace those damaged by injury or diseases. Although the mechanism of 'Plastic- ity' and its application in the regenerative medicine are still in doubt, thorough exploration in these subjects would open up broad prospects for the use in cell and tissue engineering in the near future. | WANG Yunfang & PEI Xuetao Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China Correspondence should be addressed to Pei Xuetao (e-mail: peixt@ nic.bmi.ac.cn) | 2003 | Chinese Science Bulletin2003，48，13： | 3 |
| 4 | iPS cells—Alternative pluripotent cells to embryo stem cells显示文摘Since the first murine and human embryonic stem cell lines were established by Drs. Evans and Kaufman [1] and Thomson et al. [2], respectively, great progress has been make in the field | PEI XueTao Beijing Institute of Transfusion Medicine,Beijing 100850,China | 2010 | Science China(Life Sciences)2010，53，1： | 2 |
| 5 | Guidelines for mieroplate techniques in liquid-phase blood grouping and antibody screening 显示文摘 | A Joint Publication of the British Society for Haematology and the British Blood Transfusion Society | 1990 | Clin Lab Haematol1990，12，4： | 1 |
| 6 | Transfusion guidelines for neonates and older children显示文摘 | British Committee for Standardsin Haematology Blood Transfusion Task Force | 2004 | Br J Haematol2004，124，4： | 1 |
| 7 | Practice guidelines for perioperativc blood transfusion and adjuvant therapies: an update report by the American Society of Anesthesiologists Task Force on Blood Transfusion and Adjuvant Therapies 显示文摘 | American Society of Anesthesiologists Task Force on Perioperative Blood Transfusion and Adjuvant Therapies | 2006 | Anesthesiology2006，，： | 1 |
| 8 | Guidelines for the use of platelet transfusions显示文摘 | British Committee for Standardsin Haematology Blood Transfusion Task Force | 2003 | Bri J Haematology2003，122，1： | 1 |
| 9 | Guidelines on γ irradiation of blood components for the prevention of transfusion-associated graft-versus-host disease显示文摘 | BCSH Blood Transfusion Task Force | 1996 | Transfus Med1996，6，3： | 1 |
| 10 | AmericanCollege of Physicians显示文摘 | Practice strategies for elective red blood cell transfusion | 1992 | Ann Intern Med1992，116，5： | 1 |
| 11 | Guidelines for the use of fresh-frozen plasma, cryoprecipitate and cryosupernatant 显示文摘 | British Committee for Standards in Haematology Blood Transfusion Task Force | 2004 | British Journal of Haematology2004，126，1： | 1 |
| 12 | Pathogen inactivation of labile blood products显示文摘 | Council of Europe Expert Committee in Blood Transfusion Study Group on Pathogen Inactivation of Labile Blood Components | 2001 | Transfus Med2001，11，3： | 1 |
| 13 | Guideline for the use of fresh frozen plasma 显示文摘 | Medical Directors Advisory Committee National Blood Transfusion Council | 1998 | S Afr Med J1998，88，10： | 1 |
| 14 | Practice guidelines for perioperative blood transfusion and adjuvant therapies:an updated report by the American Society of Anesthesiologists Task Force on Perioperative Blood Transfusion and Adjuvant Therapies显示文摘 | American Society of Anesthesiologists Task Force on Perioperative Blood Transfusion and Adjuvant Therapies | 2006 | Anesthesiology2006，105，： | 1 |
| 15 | Guidelines for the clinical use of red cell transfusions显示文摘 | British Committee for Standards in Haematology Blood Transfusion Task Force | 2001 | Br J Haematol2001，113，： | 1 |
| 16 | Guidelines for the use of platelet transfusion显示文摘 | Blood Transfusion Task Force | 2003 | Br J Haematol2003，122，1： | 1 |
| 17 | Practice guidelines for perioperative blood transfusion and sdjuvant therapies: sn updated report by the American society of anesthesiologists task force on perioperative blood transfusion and adjuvant therapies 显示文摘 | American Society of Anesthesiologists Task Force on Perioperative Blood Transfusion and Adjuvant Therapies | 2006 | Anesthesiology2006，105，1： | 1 |
| 18 | Guidelines for blood grouping and red cell antibody testing during pregnancy显示文摘 | British Committee for Standards in Haematology Blood Transfusion Task Force | 1996 | Transfusion Med1996，6，： | 1 |
| 19 | Guidelines for the use of fresh frozen plasma显示文摘 | British Committee for Standards in Haematology Working Party of the Blood Transfusion Task Force | 1992 | Transfusion Med1992，2，1： | 1 |
| 20 | Practice guidelines for perioperative blood transfusion and adjuvant therapies:an updated report by the A-merican Society of Anesthesiologists Task Force on Perioperative Blood Transfusion and Adjuvant Therapies显示文摘 | American Society of Anesthesiologists Task Force on Perioperative Blood Transfusion and Adjuvant Therapies | 2006 | Anesthesiology2006，105，1： | 1 |
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