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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Mobile Ubiquitous Service Environment显示文摘The Mobile Ubiquitous Service Environment (MUSE),established through the coordination and integration of mobile telecommunications and ubiquitous network,in the pursuit of Always Best Experience (ABE),represents the major development trend for the next generation mobile wireless network. Research on MUSE will involve the integration of the computing model system,service platform system,operating system and terminal structure system,all of which involve exploration and innovation of a new networking structure,its control and management as well as way of measuring. The change in network resources triggers the change in network computing models. To let readers have a basic understanding of MUSE,this lecture introduces it in four sections. This section focuses on the development and demand analysis of the service platform. | Zhang Ping,Ji Yang,Feng Zhiyong (Research Center for Wireless New Technology,Beijing University of Posts and Telecommunications,Beijing 100876,China) The Mobile Ubiquitous Service Environment (MUSE),established through the coordination and integration of mobile telecommunications and ubiquitous network,in the pursuit of Always Best Experience (ABE),represents the major development trend for the next generation mobile wireless network. Research on MUSE will involve the integration of the computing model system,service platform system,operating system and terminal structure system,all of which involve exploration and innovation of a new networking structure,its control and management as well as way of measuring. The change in network resources triggers the change in network computing models. To let readers have a basic understanding of MUSE,this lecture introduces it in four sections. This section focuses on the development and demand analysis of the service platform. | 2007 | ZTE Communications2007，5，2： | 10 |
| 3 | Current progress in epigenetic research for hepato-carcinomagenesis显示文摘Hepatocellular carcinoma is the main type of primary liver cancer,and also one of the most malignant tumors.At present,the pathogenesis mechanisms of liver cancer are not entirely clear.It has been shown that inactivation of tumor suppressor genes and activation of oncogenes play a significant role in carcinogenesis,caused by the genetic and epigenetic aberrance.In the past,people generally thought that genetic mutation is a key event of tumor pathogenesis,and somatic mutation of tumor suppressor genes is in particular closely associated with oncogenesis.With deeper understanding of tumors in recent years,increasing evidence has shown that epigenetic silencing of those genes,as a result of aberrant hypermethylation of CpG islands in promoters and histone modification,is essential to carcinogenesis and metastasis.The term epigenetics refers to heritable changes in gene expression caused by regulation mechanisms,other than changes in the underlying DNA sequence.Specific epigenetic processes include DNA methylation,genome imprinting,chromotin remodeling,histone modification and microRNA regulations.This paper reviews recent epigenetics research progress in the hepatocellular carcinoma study,and tries to depict the relationships between hepatocellular carcinomagenesis and DNA methylation as well as microRNA regulation. | HUANG Jian National Human Genome Research Center at Shanghai-Ministry of Science and Technology to build a healthy and disease Ge-nomics Laboratory,Shanghai 201203,China | 2009 | Science China(Life Sciences)2009，52，1： | 9 |
| 4 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 5 | New Development of China-Russia-India Triangle显示文摘Since 2001, positive improvement has occurred in the trianglerelationship between China, Russia and India. The foreignministers of the three nations met in Russia recently, symbolizingthe launching of substantive triangular cooperation. | Ma Jiali is Research Professor at CICIR. He specializes in South Asian security, especially in India’s foreign policy. He is the author of three books, such as Pay Close Attention to India: A Rising Power which is very influential. He was one of the delegates for Track Two Dialogue between China and India for all of four rounds and a delegate for Track Two Dialogue between China, Russia and India. He is also a member of Eminent Persons Group Forum between China and India. | 2005 | Contemporary International Relations2005，15，7： | 4 |
| 6 | Response characteristics of an ascending auditory neuron AN3 and its projections in the brain of the bushcricket Gampsocleis grationsa显示文摘The phonotaxis of bushcrickets demonstrates that they have goodcapability of recognizing conspecific songs and locating the sound source,which bases on complicated auditory mechanisms of the central nervoussystem(CNS).This article describes the response characteristics of AN3,an auditory ascending neuron in the prothoracic ganglion,and its projec-tions within the brain.The AN3,with a quasi-tonic discharge pattern,has high sensitivity,directionality and ability to encode sound intensity.Therefore,it plays an important role in auditory information processingof CNS.Its projections in the brain are mainly concentrated in the lateralzone of the deutencephalon,where synaptically connecting local auditoryinterneurons in the brain. | GAO Panpan and SHEN Jun -Xian(Institute of Biophysics, Academia Sinica, Beijing 100080)The phonotaxis of bushcrickets demonstrates that they have good capability of recognizing conspecific songs and locating the sound source, which bases on complicated auditory mechanisms of the central nervous system (CNS). This article describes the response characteristics of AN3, an auditory ascending neuron in the prothoracic ganglion, and its projections within the brain. The AN3 , with a quasi-tonic discharge pattern, has high sensitivity, directionality and ability to encode sound intensity. Therefore, it plays an important role in auditory information processing of CNS. Its projections in the brain are mainly concentrated in the lateral zone of the deutencephalon, where synaptically connecting local auditory interneurons in the brain. | 1992 | Chinese Journal of Acoustics1992，11，1： | 3 |
| 7 | The Cosmic Dust in Suzhou Granite:Ablated Products of a Cometary Explosion | Hu Zhong-wei,Wang Er-kang,Wan Yu-qiuDepartement of Astronomy,Nanjing University,Nanjing 210093,China Department of Earth Sdence,Nanjing University,Nanjing 210093,China Cosmic dust has been collected in large number from deap-sea sediments,Greenland ice deposits,the stratosphere and space,of cosmic dust,some are the unmelted micrometeorite,and most are the ablated products of me-teoroids in the atmosphere.Cosmic dusts were recovered from two sites of the 1908 Tunguska explosion and the 1965 Revelstoks explosion Since 1987,a large number of microspherules have been extracted from Suzhou granite of China Their suface and section structures,mineral and chemical composition show that these microspherules are of the ablated cosmic dust.The microspherules occurred in the inner contact complex intrusion.It suggests that the microspherules may be products of the same event and were aggergated in some strata and then captured by the emplacement of granitic magma.Although the microspherules are similare to other ablated cosmic dusts,there are some diferences.Our calculated results of cometary motion through the atmosphere and explosion show that the microspherules may be the ablated dust of a comet with mass above 1015g at its explosion near the ground. | 1996 | 紫金山天文台台刊1996，15，3： | 2 |
| 8 | BLIND AND INTERNET显示文摘BLINDANDINTERNETBLINDANDINTERNETZhaoGuanglu(ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollege,Beijing100730China)In... | Qin-zhang Ding(The Department of Neuropsychiatry.Second Affiliated Hospital of Hebei Medical UniversityShijiazhuang. Heibe, 050000 China)In china a and all of the world, it is always using cloth bands to restrain activities of thelimbs in the patient | 1995 | Chinese Journal of Biomedical Engineering(English Edition)1995，4，4： | 2 |
| 9 | Haematological manifestations in patients with severe acute respiratory syndrome:retrospective analysis显示文摘 | Wong R S Wu A To K F | 2003 | BMJ2003，326，： | 2 |
| 10 | Evidence of increasing L1014F kdr mutation frequency in Anopheles gambiae s.l.pyrethroid resistant following a nationwide distribution of LLINs by the Beninese National Malaria Control Programme显示文摘Objective:To determine the susceptibility status to pyrethroid in Anopheles gambiae s.l.(An.gambiae),the distribution of kdr'Leu-Phe'mutation in malaria vectors in Benin and to compare the current frequency of kdr'Leu-Phe'mutation to the previous frequency after long-lasting insecticide treated nets implementation.Methods:Larvae and pupae of An.gambiae s.l.mosquitoes were collected from the breeding sites in Littoral,Zou.Borgou and Alibori provinces.CDC susceptibility tests were conducted on unfed females mosquitoes aged 2-5 d old.An.gambiae mosquitoes were identified to species using PCR techniques.Molecular assays were also carried out to identify kdr mutations in individual mosquitoes.Results:The results showed that An.gambiae Malanville and Suru-lere populations were resistant to deltamethrin.Regarding An.gambiae Parakou and Bohicon populations,they were resistant to permethrin.PCR revealed 100%of mosquitoes tested were An.gambiae s.s.The L1014F kdr mutation was found in An.gambiae s.s.Malanville and Parakou at various allelic frequencies.The increase of kdr allelic frequency was positively correlated with CDC bioassays data.Conclusions:Pyrethroid resistance is widespread in malaria vector in Benin and kdr mutation is the main resistance mechanism involved.More attention may be paid for the future success of malaria control programmes based on LLINs with pyrethroids in the country. | Nazairc Azoun Rock Akpon Martin Akogbéto | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，3： | 2 |
| 11 | Acknowledgments to reviewers of World Journal of Biological Chemistry显示文摘Many reviewers have contributed their expertise and time to the peer review,a critical process to ensure the quality of World Journal of Biological Chemistry.The | Many reviewers have contributed their expertise and time to the peer review,a critical process to ensure the quality of World Journal of Biological Chemistry.The editors | 2011 | World Journal of Biological Chemistry2011，2，9： | 2 |
| 12 | Neovascular expression of E - selectin, ICAM - 1 and VCAM - 1 in human atherosclerosis and their relation to intimal leukocyte content 显示文摘 | O'Brien KD McDonald To Chait A | 1996 | Circulation1996，93，4： | 1 |
| 13 | Dose Reconstruction显示文摘 | Lloyd D C Edwards AA Protracted Overexposure to a 137Cs Source:Ⅰ | 1999 | Radiation Protection Dosimetry1999，81，2： | 1 |
| 14 | Absence of the protease inhibitor cystatin C in inflammatory cells results in larger plaque area in plaque regression of apoE-deficient mice显示文摘 | Bengtsson E To F Grubb A | 2005 | Atherosclerosis2005，180，： | 1 |
| 15 | Wavelet denoising techniques with applications to experimental geophysical data 显示文摘 | To A C Moore J R Glaser S D | 2009 | Signal Processing2009，89，2： | 1 |
| 16 | Vitamin E and vitamin C treatment improves fibrosis in patients with nonalcoholic steatohepatitis显示文摘 | Hart ison S A To rgerson S Hayashi P | 2003 | Am J Gastroenterol2003，98，： | 1 |
| 17 | Serum lipoprotein (a) level and its clinical significance in patients with systemic lupus erythematosus 显示文摘 | Differential apolipoprotein ( a ) is an independent contributor to Clin Chim Acta Sat4 RA Polat MF Taysi S | 2002 | Clin Rheumatol2002，21，6： | 1 |
| 18 | Perfectly matched multiscale simulations 显示文摘 | To A C Li S | 2005 | Phys Rev B2005，72，： | 1 |
| 19 | WUSCHEL controls meristem function by direct regulation of cytokinin-inducible response regulators 显示文摘 | Leibfried A To JP Busch W | 2005 | Nature2005，438，7071： | 1 |
| 20 | Assessment of injury to the thoracolumbar posterior ligamentous complex in the setting of normal-appearlng plain radiography显示文摘 | Using magnetic resonance injury to the posterior ligamen- a prospective comparison of the Neurosurg Spine 2010 12(4): Lee JY Vaccaro AR Schweitzer KM Jr | 2007 | Spine J2007，7，4： | 1 |
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