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| 1 | 银鲫IgM的纯化及其单克隆抗体的制备显示文摘Aeromonas hydrophila septicemia was a serious infectious disease occurred in China.The majority harmed fish was crucian carp(Carassius auratus gibelio).It was reported that immune prevention was the effect measure to prevent this disease using A.hydrophila bacterin.So it is necessary to develop monoclonal antibody(McAb) against the IgM from Crucian carp.In this study,the purification of serum immunoglobulin from Crucian carp was carried out by using Goat IgG Agarose.The concentration of purified IgM was 2.5 mg·mL-1.By using hybridoma-monoclonal antibody technology,hybridoma cell line secreted McAb against the IgM from crucian carp had been established and the characteristics of mab8 had been described.In isotyping analysis,the results showed that it belong to IgG1,it was able to recognize the heavy chain of the IgM on western blot,and did not has any cross reaction with the Ig from 19 different fish species including common carp(Cyprinus carpio),bighead carp(Aristichthys nobilis),grass carp(Ctenopharhyngodon idella) and bream(Abramis brama) etc.The results demonstrated that McAb was highly specific and sensitive to the Ig of Crucian carp,and had the great potential to be used for analyzing the structure of crucian carp IgM,monitoring the immunity level of cultured Crucian carp and pathogen diagnosis. | 沈锦玉 Thompson K D Adams A Galina Jeney 钱冬 沈智华 | 2006 | 水产学报2006，30，3： | 4 |
| 2 | Effect of deoxynivalenol on neurotransmitters in discrete regions of swine brain 显示文摘 | Prelusky D B Yeung J M Thompson B K | 1992 | Archives of Environmental Contamination and Toxicology1992，22，1： | 1 |
| 3 | Endothelial nitric oxide synthase polymorphisms do not influence pulmonary artery systolic pressure at lltitude 显示文摘 | SMITH E M D BAILLIE J K THOMPSON A A R | 2006 | High Altitude Medicine & Biology2006，7，3： | 1 |
| 4 | Effects of temperature and host maturity on lesion development of Colletotrichum graminicola on corn 显示文摘 | Leonard K Thompson D | 1976 | Phytopathology1976，，66： | 1 |
| 5 | Determination of lincomycin and tylosin residues in honey using solid - phase ex- traction and liquid chromatography - atmospheric pressure chemi- cal ionization mass spectrometry 显示文摘 | Thompson S T Noot D K Calvert J | 2003 | Journal of Chromatogra- phy A2003，1020，2： | 1 |
| 6 | Endo-scopic management of intratracheal ectopic thyroid 显示文摘 | Pritchyk K M Thompson L D Malekzadeh S | 2004 | Otolaryngol Head Neck Surg2004，130，5： | 1 |
| 7 | Lamb birth-weight is affected by culture system utilized during in-vitro pre-elongation development of ovine embryos显示文摘 | THOMPSON J G GARDNER D K PUGH P A | 1995 | BiolReprod1995，53，6： | 1 |
| 8 | Food Fun and Fintness Internet Program for girls:Influencing log-on rate显示文摘 | THOMPSON D BARANOWSKI T CULLEN K | 2008 | Health Educ Res2008，23，2： | 1 |
| 9 | Corrosion resistant coatings from conducting polyaniline 显示文摘 | Wrobleski D A BeneeewiCZ B C Thompson K G | 1994 | Polymer Preprints1994，35，： | 1 |
| 10 | Estradiol interactions with dopamine antagonists in mares:prolactin secretion and reproductive traits显示文摘 | KELLEY K K THOMPSON D L STORER W A | 2006 | J Equine Vet Sci2006，26，11： | 1 |
| 11 | Seasonal variation and the immune response:a fish perspective显示文摘 | Bowden T J Thompson K D Morgan A L | 2007 | Fish Shellfish Immunol2007，22，6： | 1 |
| 12 | Endoscopic balloon dilatation versus endoscopic sphincterotomy for the removal of bile duct stones: a prospective randomised trial显示文摘 | Vlavianos P Chopra K Mandalia S Anderson M Thompson J Westaby D | 2003 | Gut2003，52，： | 1 |
| 13 | Heat treatment of wollastonite-type Y-Al-Si-O-N glasses显示文摘 | LIDDELL K THOMPSON D P | 1997 | Journal of Materials Science1997，32，4： | 1 |
| 14 | Model for hydrocarbon microseepage and related near-surface alterations显示文摘 | Saunders D F Buraon K R Thompson C K | 1999 | AAPG Bulletin1999，83，1： | 1 |
| 15 | Specific acyclic isoprenoids as biological markers of methanogenic bacteria in marine sediments显示文摘 | Brassell S C Wardroper A M K Thompson I D | 1981 | Nature1981，290，： | 1 |
| 16 | An esti mation of the global volume of surgery: a modelling strategy based on a- vailable data显示文摘 | WEISER T G REGENBOGEN S E THOMPSON K D | 2008 | Lancet2008，32，13： | 1 |
| 17 | Toxicity of T - 2 toxin and it s interaction with deoxynivalenol when fed to young pigs 显示文摘 | Friend D W Thompson B K Trenholm H L | 1992 | Canadian Journal of Animal Science1992，72，： | 1 |
| 18 | The expression of a nopaline synthase-human growth hormone chimaeric gene in transformed tobacco and sunflower callus tissue 显示文摘 | BARTA A SOMMERGRUBER K THOMPSON D | 1986 | Plant Mol Biol1986，6，： | 1 |
| 19 | Kinetics of interfacial reaction in bimetallic Cu-Sn thin films显示文摘 | Tu K N Thompson R D | 1982 | Acta Materialia1982，30，5： | 1 |
| 20 | Observa tions on the possible origin, formation and structure of calca reous corpuscles in taeniid cestodes显示文摘 | PAWLOWSKI I D YAP K W THOMPSON R C | 1988 | Parasitol Res1988，74，3： | 1 |
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