| 6 | Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hvbridization显示文摘RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed. | QIU Jiwen, JIN Demin, LI Chuanyou, JIA Jianhang, OUYANG Ping William TAI, WANG Bin & LI Dawei1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics, Chinese Academy of Sciences, Beijing,100101.China 2. Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China 3. Institute for Global Chinese Affairs, University of Maryland, USA * Jin Deming did the same work as the first author Correspondence should be addressed to Wang Bin & Li Dawei | 2000 | Chinese Science Bulletin2000，45，6： | 0 |
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