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| 1 | Gene expression profiling of MYC-driven tumor signatures in porcine liver stem cells by transcriptome sequencing显示文摘AIM:To identify the genes induced and regulated by the MYC protein in generating tumors from liver stem cells.METHODS:In this study,we have used an immortal porcine liver stem cell line,PICM-19,to study the role of c-MYC in hepatocarcinogenesis.PICM-19 cells were converted into cancer cells(PICM-19-CSCs)by overexpressing human MYC.To identify MYC-driven differential gene expression,transcriptome sequencing was carried out by RNA sequencing,and genes identified by this method were validated using real-time PCR.In vivo tumorigenicity studies were then conducted by injecting PICM-19-CSCs into the flanks of immunodeficient mice.RESULTS:Our results showed that MYC-overexpressing PICM-19 stem cells formed tumors in immunodeficient mice demonstrating that a single oncogene was sufficient to convert them into cancer cells(PICM-19-CSCs).By using comparative bioinformatics analyses,we have determined that>1000 genes were differentially expressed between PICM-19 and PICM-19-CSCs.Gene ontology analysis further showed that the MYCinduced,altered gene expression was primarily associated with various cellular processes,such as metabolism,cell adhesion,growth and proliferation,cell cycle,inflammation and tumorigenesis.Interestingly,six genes expressed by PICM-19 cells(CDO1,C22orf39,DKK2,ENPEP,GPX6,SRPX2)were completely silenced after MYC-induction in PICM-19-CSCs,suggesting that the absence of these genes may be critical for inducingtumorigenesis.CONCLUSION:MYC-driven genes may serve as promising candidates for the development of hepatocellular carcinoma therapeutics that would not have deleterious effects on other cell types in the liver. | Rajagopal N Aravalli Neil C Talbot Clifford J Steer | 2015 | World Journal of Gastroenterology2015，21，7： | 3 |
| 2 | A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection显示文摘 | VIAUD M C BALHDERE P V TALBOT N J | 2002 | The Plant Cell2002，14，： | 1 |
| 3 | DNA immunization of dairy cows with the clumping factor A of Staph- ylococcus aureus显示文摘 | ADEL N M NOUR EL-DIN LULZIM SHKRETA BRIAN G TALBOT | 2006 | Vaccine2006，24，： | 1 |
| 4 | Positron emission tomography with -FDG in oncology 显示文摘 | Talbot J N Petegnief Y Kerrou K | 2003 | Nuclear Instruments and Methods in Physics Research A2003，504，13： | 1 |
| 5 | The genome se- quence of the rice blast fungus Magnaporthe grisea显示文摘 | Dean R A Talbot N J Ebbole D J | 2005 | Nature2005，434，： | 1 |
| 6 | Under pressure:investigating the biology of plant infection by Magnaporthe oryzae显示文摘 | WILSON R A TALBOT N J | 2009 | Nature Reviews Microbiology2009，7，3： | 1 |
| 7 | The genome sequence of the rice blast fungus Magnaporthe grisea显示文摘 | Dean R A Talbot N J Ebbole D J | 2005 | Nature2005，434，7036： | 1 |
| 8 | The biology of blast: Understanding how Magnaporthe oryzae invades rice plants显示文摘 | Galhano R Talbot N J | 2011 | Fungal Biology Reviews2011，25，1： | 1 |
| 9 | Fast exact leave-one-out Cross- Validation of sparse least-squares support vector machines显示文摘 | CAWLEY G C TALBOT N L | 2004 | Neural Networks2004，17，： | 1 |
| 10 | On the trail of a cereal killer:exploring the biology of magnaporthe grisea显示文摘 | Talbot N J | 2003 | Annu Rev Microbiol2003，57，： | 1 |
| 11 | On the trail of a cereal killer:Exploring the biology of Magnaporthe grisea显示文摘 | TALBOT N J | 2003 | Annual Review of Microbiology2003，57，： | 1 |
| 12 | Autophagic fungal cell death is necessary for infection by the rice blast fungus显示文摘 | Veneault-Fourrey C Barooah M Egan M Wakley G Talbot N J | 2006 | Science2006，312，： | 1 |
| 13 | Comparison of immu-nofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture显示文摘 | Arbour N Talbot PJ | 1998 | J Virol Methods1998，72，2： | 1 |
| 14 | Genetic control of incubation behavior in the domestic hen显示文摘 | Romanov M N Talbot R T Wilson P W | 2002 | Poultry Science2002，81，7： | 1 |
| 15 | On the trail of a cereal killer: Exploring the biology of Magnaporthe grisea显示文摘 | Talbot N J | 2003 | Annual Review of Microbiology2003，57，： | 1 |
| 16 | On the trail of a cereal killer:exploring the biology of Magnaporthe grisea 显示文摘 | Talbot N J | 2003 | Annu Rev Microbiol2003，57，： | 1 |
| 17 | Comparative genomic analysis of phytopathogenic fungi using expressed sequence tag (EST) col- lections显示文摘 | SOANES D M Talbot N J | 2006 | Molecular Plant Pathology2006，7，1： | 1 |
| 18 | The genome sequence of the rice blast fungus Magnaporthe grisea显示文摘 | Dean R A Talbot N J Ebbole D J | 2005 | Nature2005，434，7036： | 1 |
| 19 | Deletions in the survival motor neuron gene on 5 q13 in autosomal recessive spinal muscular atrophy显示文摘 | Rodrigues NR Owen N Talbot K | 1995 | Hum Mol Genet1995，4，4： | 1 |
| 20 | Superoxide activates a GDPsensitive proton conductance in skeletal muscle mitochandria from king penguin显示文摘 | Talbot DA Hanuise N Rey B | 2003 | Biochem Biophys Res Commun2003，312，4： | 1 |
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