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| 1 | Micro RNA-320 family is downregulated in colorectal adenoma and affects tumor proliferation by targeting CDK6显示文摘AIM: To investigate the microR NA(miR NA) expression during histological progression from colorectal normal mucosa through adenoma to carcinoma within a lesion. METHODS: Using microarray, the sequential changes in miR NA expression profiles were compared in colonic lesions from matched samples; histologically, nonneoplastic mucosa, adenoma, and submucosal invasive carcinoma were microdissected from a tissue sample. Cell proliferation assay was performed to observe the effect of miR NA, and its target genes were predicted using bioinformatics approaches and the expression profile of SW480 transfected with the miR NA mimics. mR NA and protein levels of the target gene in colon cancer cell lines with a mimic control or miR NA mimics were measured using qR TPCR and Western blotting. The expression levels of miR NA and target gene in colorectal tissue samples were also measured.RESULTS: Microarray analysis identified that the miR-320 family, including miR-320 a, miR-320 b, miR-320 c, miR-320 d and miR-320 e, were differentially expressed in adenomaand submucosal invasive carcinoma. The mi R-320 family, which inhibits cell proliferation, is frequently downregulated in colorectal adenoma and submucosal invasive carcinoma tissues. Seven genes including CDK6 were identified to be common in the results of gene expression array and bioinformatics analyses performed to find the target gene of the miR-320 family. We confirmed that mR NA and protein levels of CDK6 were significantly suppressed in colon cancer cell lines with miR-320 family mimics. CDK6 expression was found to increase from non-neoplastic mucosa through adenoma to submucosal invasive carcinoma tissues and showed an inverse correlation with miR-320 family expression.CONCLUSION: MiR-320 family affects colorectal tumor proliferation by targeting CDK6, plays important role in its growth, and is considered to be a biomarker for its early detection. | Toshihiro Tadano Yoichi Kakuta Shin Hamada Yosuke Shimodaira Masatake Kuroha Yoko Kawakami Tomoya Kimura Hisashi Shiga Katsuya Endo Atsushi Masamune Seiichi Takahashi Yoshitaka Kinouchi Tooru Shimosegawa | 2016 | World Journal of Gastrointestinal Oncology2016，8，7： | 9 |
| 2 | Induction of nociceptive responses by intrathecal injection of interleukin-1 in mice显示文摘 | Tadano T Namioka M Nakagawasai O | 1999 | Life Sci1999，65，3： | 1 |
| 3 | Effect of metal cation type on the structure and properties of ethylene ionomers 显示文摘 | Hirasawa E Yamamoto Y Tadano Y Yano S | 1991 | J Appl Polym Sci1991，42，： | 1 |
| 4 | Health effects of in- door fluoride pollution from coal burning in China显示文摘 | Ando M Tadano M Asanuma S | 1998 | En- vironmental Health Perspectives1998，106，5： | 1 |
| 5 | A weighted block GMRES method for solving linear systems with multiple right-hand sides 显示文摘 | Imakura A Du L Tadano H | 2013 | Japan Society for Industrial and Applied Mathematics2013，5，： | 1 |
| 6 | Health effects of fluoride pollution caused by coal burning 显示文摘 | Mitsuru Ando Mihoko Tadano Shoji Yamamoto | 2001 | The Science of the Total Environment2001，271，2001： | 1 |
| 7 | A Mathematical Expres-sion of Three-Dimensional Configuration of the Seoliotic Spine 显示文摘 | Kanayama M Tadano S Kaneda K | 1996 | Journal of Biomechanical Engineering1996，9，： | 1 |
| 8 | Influence of disc degeneration on mechanism of thoracolumbar burst fractures 显示文摘 | Shirado O Kaneda K Tadano S | 1992 | Spine1992，17，： | 1 |
| 9 | Ontogenetic changes in the contents of ribulose-1, 5-bisphophate carboxyase/oxygenase, phos- phoenolpyruvate carboxylase, and chlorophyll in individual leaves of maize显示文摘 | Osaki M Iyoda M Tadano T | 1995 | Soil Science Plant Nutrition1995，41，2： | 1 |
| 10 | Serection of acid phosphatase by roots of sereral crop species under phosphorus-deficient conditions显示文摘 | TADANO T SAKAI H | 1991 | Soil Sci Plant Nutr1991，37，1： | 1 |
| 11 | Pedistribution of carbon and nitrogen compounds from the shoot to the harvesting organs durjng maturation in field crops显示文摘 | OSAKI M SHINANO T TADANO T | 1991 | Soil Science and Plant Nutrition1991，37，1： | 1 |
| 12 | Locus of a virus neutralization epitope on the Japanese encephalitis virus envelope protein determined by use of long PCR-based region-specific random mutagenesis显示文摘 | MORITA K TADANO M NNKAJI S | 2001 | Virology2001，287，2： | 1 |
| 13 | Effect of nitrogen application on reconstruction of mitrogen compounds during the maturation stage in several fiel crops显示文摘 | Shinano T Osaki M Tadano T | 1991 | Soil Sci &Plant Nutr1991，37，2： | 1 |
| 14 | Effects of GABA supple- mentation on blood pressure and safety in adults with mild hyperten- sion显示文摘 | Matsubara H Tadano K Suyama T | 2002 | Yakuri to chiryo2002，30，11： | 1 |
| 15 | Selection of acid phosphatase by the roots of several crop speciesunder phosphorus-deficient conditions 显示文摘 | Tadano T Sakai H | 1991 | Soil Sciences and Plant Nutrition1991，37，1： | 1 |
| 16 | Indicators and thresholds for the evaluation of sustainable land management显示文摘 | Syers J K Tadano T Sakai H | 1995 | Canadian journal of Soil Science1995，75，2： | 1 |
| 17 | Secretion of acid phosphatase by the roots of sev- eral crop species under phosphorus-deficient conditions显示文摘 | Tadano T Sakia H | 1991 | Soil Sci- ence and Plant Nutrition1991，37，1： | 1 |
| 18 | Intraplantar injection of gan- gliosides produces nociceptive behavior and hyperalgesia via a gluta- mate signaling mechanism显示文摘 | Watanabe S Tan-No K Tadano T l | 2011 | Pain2011，152，2： | 1 |
| 19 | Health effects of fluoride pollution caused by coal burning 显示文摘 | Ando M Tadano M Yamamoto S | 2001 | Sci Total Environ2001，271，13： | 1 |
| 20 | Normally-off type high speed SI-thyristor 显示文摘 | NAKAMURA Y TADANO H SUGIYAMA S | 1982 | IEEE Transactions on Electron Devices1982，28，4： | 1 |
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