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| 1 | Golgi protein-73:A biomarker for assessing cirrhosis and prognosis of liver disease patients显示文摘BACKGROUND Reliable biomarkers of cirrhosis,hepatocellular carcinoma(HCC),or progression of chronic liver diseases are missing.In this context,Golgi protein-73(GP73)also called Golgi phosphoprotein-2,was originally defined as a resident Golgi type II transmembrane protein expressed in epithelial cells.As a result,GP73 expression was found primarily in biliary epithelial cells,with only slight detection in hepatocytes.However,in patients with acute or chronic liver diseases and especially in HCC,the expression of GP73 is significantly up-regulated in hepatocytes.So far,few studies have assessed GP73 as a diagnostic or prognostic marker of liver fibrosis and disease progression.AIM To assess serum GP73 efficacy as a diagnostic marker of cirrhosis and/or HCC or as predictor of liver disease progression.METHODS GP73 serum levels were retrospectively determined by a novel GP73 ELISA(QUANTA Lite®GP73,Inova Diagnostics,Inc.,Research Use Only)in a large cohort of 632 consecutive patients with chronic viral and non-viral liver diseases collected from two tertiary Academic centers in Larissa,Greece(n=366)and Debrecen,Hungary(n=266).Aspartate aminotransferase(AST)/Platelets(PLT)ratio index(APRI)was also calculated at the relevant time points in all patients.Two hundred and three patients had chronic hepatitis B,183 chronic hepatitis C,198 alcoholic liver disease,28 autoimmune cholestatic liver diseases,15 autoimmune hepatitis,and 5 with other liver-related disorders.The duration of follow-up was 50(57)mo[median(interquartile range)].The development of cirrhosis,liver decompensation and/or HCC during follow-up were assessed according to internationally accepted guidelines.In particular,the surveillance for the development of HCC was performed regularly with ultrasound imaging and alpha-fetoprotein(AFP)determination every 6 mo in cirrhotic and every 12 mo in non-cirrhotic patients.RESULTS Increased serum levels of GP73(>20 units)were detected at initial evaluation in 277 out of 632 patients(43.8%).GP73-seropositivity correlated at baseline with the presence of cirrhosis(96.4%vs 51.5%,P<0.001),decompensation of cirrhosis(60.3%vs 35.5%,P<0.001),presence of HCC(18.4%vs 7.9%,P<0.001)and advanced HCC stage(52.9%vs 14.8%,P=0.002).GP73 had higher diagnostic accuracy for the presence of cirrhosis compared to APRI score[Area under the curve(AUC)(95%CI):0.909(0.885-0.934)vs 0.849(0.813-0.886),P=0.003].Combination of GP73 with APRI improved further the accuracy(AUC:0.925)compared to GP73(AUC:0.909,P=0.005)or APRI alone(AUC:0.849,P<0.001).GP73 levels were significantly higher in HCC patients compared to non-HCC[22.5(29.2)vs 16(20.3)units,P<0.001)and positively associated with BCLC stage[stage 0:13.9(10.8);stage A:17.1(16.8);stage B:19.6(22.3);stage C:32.2(30.8);stage D:45.3(86.6)units,P<0.001]and tumor dimensions[very early:13.9(10.8);intermediate:19.6(18.4);advanced:29.1(33.6)units,P=0.004].However,the discriminative ability for HCC diagnosis was relatively low[AUC(95%CI):0.623(0.570-0.675)].Kaplan-Meier analysis showed that the detection of GP73 in patients with compensated cirrhosis at baseline,was prognostic of higher rates of decompensation(P=0.036),HCC development(P=0.08),and liver-related deaths(P<0.001)during follow-up.CONCLUSION GP73 alone appears efficient for detecting cirrhosis and superior to APRI determination.In combination with APRI,its diagnostic performance can be further improved.Most importantly,the simple GP73 measurement proved promising for predicting a worse outcome of patients with both viral and nonviral chronic liver diseases. | Nikolaos K Gatselis Tamás Tornai Zakera Shums Kalliopi Zachou Asterios Saitis Stella Gabeta Roger Albesa Gary L Norman Mária Papp George N Dalekos | 2020 | World Journal of Gastroenterology2020，26，34： | 21 |
| 2 | Activation of human fibroblast-like synoviocytes by uric acid crystals in rheumatoid arthritis显示文摘Hyperuricemia-mediated uric acid crystal formation may cause joint inflammation and provoke the destruction of joints through the activation of inflammasome-mediated innate immune responses.However,the immunopathological effects and underlying intracellular regulatory mechanisms of uric acid crystal-mediated activation of fibroblast-like synoviocytes(FLS)in rheumatoid arthritis(RA)have not been elucidated.Therefore,we investigated the in vitro effects of monosodium urate crystals,alone or in combination with the inflammatory cytokines tumor-necrosis factor(TNF)-a or interleukin(IL)-1b,on the activation of human FLS from RA patients and normal control subjects and the underlying intracellular signaling mechanisms of treatment with these crystals.Monosodium urate crystals were able to significantly increase the release of the inflammatory cytokine IL-6,the chemokine CXCL8 and the matrix metalloproteinase(MMP)-1 from both normal and RA-FLS(all P,0.05).Moreover,the additive or synergistic effect on the release of IL-6,CXCL8 and MMP-1 from both normal and RA-FLS was observed following the combined treatment with monosodium urate crystals and TNF-a or IL-1b.Further experiments showed that the release of the measured inflammatory cytokine,chemokine and MMP-1 stimulated by monosodium urate crystals were differentially regulated by the intracellular activation of extracellular signal-regulated kinase and c-Jun N-terminal kinase pathways but not the p38 mitogen-activated protein kinase pathway.Our results therefore provide a new insight into the uric acid crystal-activated immunopathological mechanisms mediated by distinct intracellular signal transduction pathways leading to joint inflammation in RA. | Da P Chen Chun K Wong Lai S Tam Edmund K Li Christopher WK Lam | 2011 | Cellular & Molecular Immunology2011，8，6： | 5 |
| 3 | 查看详情显示文摘 | W C Huang H Y Tam P K A Wai X Y Dong H Ming J P Xie | | 中国物理快报(英文版)0，，： | 4 |
| 4 | 利钠肽受体2的缺乏促进小鼠二叶主动脉瓣、主动脉瓣疾病、左心室功能不全以及升主动脉扩张显示文摘主动脉瓣疾病是无有效药物治疗的细胞介导的病变过程。C型利钠肽(C-type natriuretic peptide,CNP)抑制瓣膜间质细胞(valve interstitial cells,VIC)的肌纤维形成和成骨过程,并且在出现主动脉瓣狭窄时水平下调。然而,CNP信号是否调节体内主动脉瓣的健康状态尚不清楚。该研究旨在确定小鼠CNP信号通路的缺失是否导致主动脉瓣疾病加速发展。 | 刘青 叶鹏 Blaser MC Wei K Adams RL Zhou YQ Caruso LL Mirzaei Z Lam A Tam RK Zhang H Heximer SP Henkelman M Simmons CA | 2018 | 中华高血压杂志2018，26，1： | 3 |
| 5 | Antioxidant potential,tannin and polyphenol contents of seed and pericarp of three Coffea species显示文摘Objective:To investigate the antioxidant activity,total phenolic and total tannin content of the pericarp and the seed of Coffea benghalensis(C.benghalensis) and Coffea liberica compared to Coffea arabica(C.arabica).Methods:The antioxidant potential,total tannin and polyphenol contents of the immature and mature seed and pericarp of C.benghalensis and Coffea liberica were quantified and compared to C.arabica.Enhanced chemiluminescence(ECL).2,2-diphenyl-1-picrylhydruzyl(DPPH).oxygen radical absorbance capacity,Folin-Ciocaltcau method and total tannin content assays were used.Results:Trolox equivalent(TE/g plant material) values obtained by ECL and DPPH methods showed loose correlation(r^2=0.587)while those measured by oxygen radical absorbance capacity assay were higher without correlation in each plant.A closer correlation was detected between the ECL method and the percentage antioxidant activity of the DPPH technique(r^2=0.610 7) in each species,however the immature pericarp of C.benghalensis showed much higher DPPH scavenging potential than was seen in the ECL assay.The immature pericarp of C.benghalensis expressed the highest tannin and polyphenol content,and a high polyphenol level was also detected in the immature seed of C.arabica.The immature pericarp of Bengal and Liberian coffees showed the largest amount of phenolic contents.Conclusions:The obtained data highlight the potential role of C.benghalensis as a new source of natural antioxidants and polyphenols compared to C.arabica. | va Brigitta Patay Nikolett Sali Tams Kszegi Rita Csepregi Viktória Lilla Blaz Tibor Sebastian Németh Tibor Németh Nóra Papp | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，4： | 3 |
| 6 | Dispersion-relation-preserving schemes for computational aeroacoustics 显示文摘 | Tam C K Webb J C | 1993 | J Computional Physics1993，107，： | 2 |
| 7 | A study of the short wave components in computational acoustics 显示文摘 | Tam C K W Webb J C Dong Z | 1993 | Journal of Computational Acoustics1993，1，： | 2 |
| 8 | Numerical Simulation of the Generation of Axisymmetric Mode Jet Screech Tones显示文摘 | Hao Shen Tam C K W | 1998 | AIAA Journal1998，36，0： | 2 |
| 9 | Wall Boundary Conditions for High- order Finite- difference Schemes in Computational Aeroacoustics 显示文摘 | Tam C K W Dung Z | 1994 | Theoretical and Computational Fluid Dynamics1994，6，56： | 1 |
| 10 | Hyperproduction of IL-23 and IL- 17 in patients with systemic lupus erythematosus: Implications for Thl7- mediated inflammation in autoimmunity 显示文摘 | Wang C K Lit L C W Tam L S | 2008 | Clin Immunol2008，127，3： | 1 |
| 11 | Modification of Hough Transform for circles and ellipses detection using a 2-dimensional array 显示文摘 | Raymond K K Yip Peter K S Tam Dennis N K Leung | 1992 | Pattern Recognition1992，25，9： | 1 |
| 12 | Non-structural Fuzzy Decision Support System for Evaluation of Construction Safety Management System显示文摘 | Tam C M Tong Thomas K L Chiu Gerald C W Fung Ivan W H | 2002 | International Journal of Project Management2002，，20： | 1 |
| 13 | Robust eigenstructure assignment under regional pole constaints显示文摘 | TAM H K LAM J | 1996 | IEEE1996，，12： | 1 |
| 14 | Deformable Model Retrieval Based on Topological and Geometric Signatures 显示文摘 | Gary K L Tam Rynson W H Lau | 2007 | IEEE Transactions on Visualization and Computer Graphics (S1077-2626)2007，13，3： | 1 |
| 15 | Collision risk assessment for ships 显示文摘 | TAM C K | 2010 | Jour- nal of Marine Science and Technology2010，15，3： | 1 |
| 16 | Association behavior of poly( methacrylic acid)-block-poly( methyl methacrylate) in aqueous medium : potentiometric and laser light scattering studies显示文摘 | Ravi P WANG C Tam K C | 2003 | Macromolecules2003，36，： | 1 |
| 17 | Cholangitis After Hepatic Portoenterostomy For Biliary Atresia:A Multivariate Analysis Of Risk Factors显示文摘 | Tam H | 2003 | J Pediatr2003，142，： | 1 |
| 18 | Peptide dendrimers: applications and syn- thesis 显示文摘 | SADLER K TAM JP | 2002 | J Biotechnol2002，90，34： | 1 |
| 19 | A biological survey of' ballast water in container ships entering Hong Kong显示文摘 | CHU K H TAM P F FUNG C H | 1997 | Hydrobiologia1997，352，1： | 1 |
| 20 | Effects of Matrine on proliferation and differentiation in K-562 cells显示文摘 | ZHANG L P JIANG J K TAM J W | 2001 | Leuk Res2001，25，9： | 1 |
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