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| 1 | Tyrosine kinase of insulin-like growth factor receptor as target for novel treatment and prevention strategies of colorectal cancer显示文摘AIM: To investigate the antineoplastic potency of the novel insulin-like growth factor 1 receptor (IGF-1R) tyro- sine kinase inhibitor (TKI) NVP-AEW541 in cell lines and primary cell cultures of human colorectal cancer (CRC). METHODS: Cells of primary colorectal carcinomas were from 8 patients. Immunostaining and crystal violet stain- ing were used for analysis of growth factor receptor pro- tein expression and detection of cell number changes, respectively. Cytotoxicity was determined by measuring the release of the cytoplasmic enzyme lactate dehydro- genase (LDH). The proportion of apoptotic cells was determined by quantifying the percentage of sub-G1 (hypodiploid) cells. Cell cycle status reflected by the DNA content of the nuclei was detected by flow cytometry. RESULTS: NVP-AEW541 dose-dependently inhibited the proliferation of colorectal carcinoma cell lines and primary cell cultures by inducing apoptosis and cell cycle arrest. Apoptosis was characterized by caspase-3 activa- tion and nuclear degradation. Cell cycle was arrested at the G1/S checkpoint. The NVP-AEW541-mediated cell cycle-related signaling involved the inactivation of Akt and extracellular signal-regulated kinase (ERK) 1/2, the upregulation of the cyclin-dependent kinase inhibitors p21Waf1/CIP1 and p27Kip1, and the downregulation of the cell cycle promoter cyclin D1. Moreover, BAX was upregu- lated during NVP-AEW541-induced apoptosis, whereas Bcl-2 was downregulated. Measurement of LDH release showed that the antineoplastic effect of NVP-AEW541 was not due to general cytotoxicity of the compound. However, augmented antineoplastic effects were ob-served in combination treatments of NVP-AEW541 with either 5-FU, or the EGFR-antibody cetuximab, or the HMG-CoA-reductase inhibitor fluvastatin. CONCLUSION: IGF-1R-TK inhibition is a promising novel approach for either mono- or combination treatment strategies of colorectal carcinoma and even for CRC che- moprevention. | Michael Hpfner Andreas P Sutter Alexander Huether Viola Baradari Hans Scherübl | 2006 | World Journal of Gastroenterology2006，12，35： | 10 |
| 2 | Signaling pathways involved in the inhibition of epidermal growth factor receptor by erlotinib in hepatocellular cancer显示文摘瞄准:在肝细胞癌(HCC ) 检验导致 erlotinib 的生长抑制的内在的机制。方法:在基因表示的导致 Erlotinib 的改变用 cDNA 数组技术被评估;在蛋白质表示或蛋白质激活的变化用西方的弄污由于象 IGF-1-induced EGFR transactivation 一样的 erlotinib 治疗被调查。结果:Erlotinib 治疗禁止了 mitogen 激活和抄写(STAT ) 的激活的蛋白质(地图)-kinase 小径和信号变换器调停了表明哪个导致了调整由 cDNA 数组技术示威了的基因的 apoptosis 和房间周期的一个改变的表达式。象与象 Bcl-2, Bcl-X (L) 或 jun D 一样的 antiapoptotic 因素的一条下面规定联系的 caspases 和 gadds 一样的 proapoptotic 因素的 Overexpression 说明了让 erlotinib 的力量导致 apoptosis。支持 G1/S-transition 的房间周期管理者并且在 cyclin 依赖的激酶禁止者和 gadds 的表示上的 Downregulation 响应 erlotinib 贡献了 G1/G0-arrest 的正式就职。而且,我们显示了由 IGF-1-receptor 的调停 EGFR 的发信号的 transactivation 并且在受体受体十字谈话显示出 erlotinib 的禁止的效果。结论:我们的学习在 HCC 房间和 thus 使 EGFR-TK-inhibition 的行动的机制的理解清楚些可能便于 additively 或 synergistically 行动的联合治疗的设计。而且,我们对 erlotinib 处理作出回应的小径上的数据能在以后预言肿瘤的应答的海角到 EGFR-TKIs 是有用的。 | Alexander Huether Michael Hpfner Andreas P Sutter Viola Baradari Detlef Schuppan Hans Scherübl | 2006 | World Journal of Gastroenterology2006，12，32： | 7 |
| 3 | Fibrinogen and C-reactive protein on admission as markers of finaI infarct size atter primary angioplasty for acute myocardial infarction显示文摘 | De Buyzere M Gheeraert P | 2001 | Atheroselerosis2001，157，1： | 1 |
| 4 | A real-life prospective health economic study of elective single embryo transfer versus two-embryo transfer in first IVF/ICSI cycles显示文摘 | Gerris J De Sutter P De Neubourg D | 2004 | Hum Reprod2004，19，4： | 1 |
| 5 | Fluorescent insitu hybridization on human embryos showing cleavage arrest after freezing and thawing显示文摘 | Laverge H Van der Elst J De Sutter P | 1998 | Hum Reprod1998，13，： | 1 |
| 6 | Treatment option for sperm-or oocyte-related fertilization failure :assisted oovyte activation following diagnositic heterologous ICSI显示文摘 | Heindryckx B Vander J Elst De Sutter P | 2005 | Hunman Reproduction2005，20，8： | 1 |
| 7 | Double-quality control reveals high-level toxicity in gloves used for operator pro- tection in assisted reproductive technology 显示文摘 | LIERMAN S DE SUTTER P DHONT M | 2007 | Fertil Steril2007，88，4： | 1 |
| 8 | An update on surgical and non-surgical treatments for vaginal hypoplasia显示文摘 | Callens N De Cuypere G De Sutter P | 2014 | Hum Repord Update2014，20，5： | 1 |
| 9 | Group decision making under risk: An experiment with student couples显示文摘 | He H Martinsson P Sutter M | 2012 | Economics Letters2012，117，3： | 1 |
| 10 | The frozen embryo and its nonresponding parents显示文摘 | Provoost V Pennings G De Sutter P | 2011 | Fertil Steril2011，95，6： | 1 |
| 11 | Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmic sperm injection 显示文摘 | De Sutter P Dozortsev D Qian C | 1996 | Hum Reprod1996，11，3： | 1 |
| 12 | Modular multiplication and exponentiation architectures for fast RSA cryptosystem based on digit serial computation 显示文摘 | Sutter G D Deschamps J P Imana J L | 2011 | Industrial Electronics2011，58，7： | 1 |
| 13 | Recombinant modified vaccinia virus Ankara expressing glycoprotein E2 of cbikungunya virus protects AG129 mice against lethal challenge 显示文摘 | van den Doel P Volz A Roose JM Sewbalaksing VD Pijlman GP van Middelkoop 1 Duiverman V van de Wetering E Sutter G Osterhaus AD Martina BE | 2014 | PLoS Negl Trop Dis2014，8，9： | 1 |
| 14 | Posterior reversible encephalopathy as the initial manifestation of a Guillain-Barre syndrome 显示文摘 | Sutter R Mengiardi B Lyrer P | 2009 | Neuromuscul Disord2009，19，： | 1 |
| 15 | Extracellular nucleotides inhibit growth of hu man oesophageal cancer cells via P2Y ( 2 )-receptors 显示文摘 | Maaser K Hopfner M Kap H Sutter A P Barthel B yon Lampe B | 2002 | Br J Cancer2002，86，： | 1 |
| 16 | Epitaxial graphene on ruthenium显示文摘 | Sutter P W Flege J I Sutter E A | 2008 | Nature materials2008，7，5： | 1 |
| 17 | Adjuvant-inducedarthritis in rats : a possible animal model of chronic pain 显示文摘 | De Castro C M De Sutter P Gybels J | 1981 | Pain1981，10，2： | 1 |
| 18 | Epitaxial graphene onruthenium显示文摘 | Sutter P W Flege J I Sutter E A | 2008 | Nature Mater2008，7，5： | 1 |
| 19 | Low grade endometrial stromal sarcoma treated with the aromatase inhibitor letrozole 显示文摘 | Leunen M Breugelmans M De Sutter P | 2004 | Gynecol Oncol2004，95，3： | 1 |
| 20 | Treatment option for sperm-or oocyte-related fertilization failure:assisted oocyte activation following diagnostic heterologous ICSI显示文摘 | Heindryckx B Van der Elst J De Sutter P | 2005 | Hum Reprod2005，20，8： | 1 |
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