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| 1 | Relocation of the 10 March 2011 Yingjiang,China,earthquake sequence and its tectonic implications显示文摘An earthquake with MS5.8 occurred on 10 March 2011 in Yingjiang county,western Yunnan,China.This earthquake caused 25 deaths and over 250 injuries.In order to better understand the seismotectonics in the region,we collected the arrival time data from the Yunnan seismic observational bulletins during 1 January to 25 March 2011,and precisely hand-picked the arrival times from high-quality seismograms that were recorded by the temporary seismic stations deployed by our Institute of Crustal Dynamics,China Earthquake Administration.Using these arrival times,we relocated all the earthquakes including the Yingjiang mainshock and its aftershocks using the double-difference relocation algorithm.Our results show that the relocated earthquakes dominantly occurred along the ENE direction and formed an upside-down bow-shaped structure in depth.It is also observed that after the Yingjiang mainshock,some aftershocks extended toward the SSE over about 10 km.These results may indicate that the Yingjiang mainshock ruptured a conjugate fault system consisting of the ENE trending Da Yingjiang fault and a SSE trending blind fault.Such structural features could contribute to severely seismic hazards during the moderate-size Yingjiang earthquake. | Jianshe Lei Guangwei Zhang Furen Xie Yuan Li Youjin Su Lifang Liu Honghu Ma Junwei Zhang | 2012 | Earthquake Science2012，25，1： | 35 |
| 2 | Outbreak of Dengue Fever in Central China,2013显示文摘In 2013,the first dengue fever(DF) outbreak in central China was reported in the central of Henan province,northern temperate regions,although they have been sequentially recorded in Southern China.106 suspected DF cases were reported and 73 patients were confirmed dengue virus type 3(DEN-3) infections.62/392(15.8%) local health persons showed DEN antibodies positive.To this day Henan is the northernmost province in China which has been reported about outbreak of DF and what is important is that it warns us the endemic range of DF has been expanded geographically in China. | HUANG Xue Yong MA Hong Xia WANG Hai Feng DU Yan Hua SU Jia LI Xing Le TANG Xiao Yan MA Hong Peng ZU Bing Can ZHANG Qiao Hong CHEN Hao Min XU Bian Li | 2014 | Biomedical and Environmental Sciences2014，27，11： | 30 |
| 3 | Application of yolk-shell Fe3O4@N-doped carbon nanochains as highly effective microwave-absorption material显示文摘 | Mingtao Qiao Xingfeng Lei Yong Ma Lidong Tian Xiaowei He Kehe Su Qiuyu Zhang | 2018 | Nano Research2018，11，3： | 26 |
| 4 | Validation of quantitative computed tomography-derived areal bone mineral density with dual energy X-ray absorptiometry in an elderly Chinese population显示文摘 | Cheng Xiaoguang Wang Ling Wang Qianqian Ma Yimin Su Yongbin Li Kai | 2014 | Chinese Medical Journal2014，，8： | 22 |
| 5 | Compensation trends of the angulation of first molars:retrospective study of 1403 malocclusion cases显示文摘We investigated the compensatory trends of mesiodistal angulation of first molars in malocclusion cases. We compared differences in the angulation of first molars in different developmental stages, malocclusion classifications and skeletal patterns. The medical records and lateral cephalogrammes of 1 403 malocclusion cases taken before treatment were measured to evaluate compensation of molar angulation in relation to the skeletal jaw. The cases were stratified by age, Angle classification and skeletal patterns. Differences in the mesiodistal angulation of the first molars were compared among the stratifications. We observed three main phenomena. First,angulation of the upper first molar varied significantly with age and tipped most distally in cases aged,12 years and least distally in cases aged.16 years. The lower first molar did not show such differences. Second, in Angle Class II or skeletal Class II cases, the upper first molar was the most distally tipped, the lower first molar was the most mesially tipped, and opposite angulation compensation was observed in Class III cases. Third, in high-angle cases, the upper and lower first molars were the most distally tipped, and opposite angulation compensation was observed in low-angle cases. These data suggest that the angulation of the molars compensated for various growth patterns and malocclusion types. Hence, awareness of molar angulation compensation would help to adjust occlusal relationships, control anchorage and increase the chances of long-term stability. | Hong Su Bing Han Sa Li Bin Na Wen Ma Tian-Min Xu | 2014 | International Journal of Oral Science2014，6，3： | 22 |
| 6 | Individualized prediction of perineural invasion in colorectal cancer: development and validation of a radiomics prediction model显示文摘Objective: To develop and validate a radiomics prediction model for individualized prediction of perineural invasion(PNI) in colorectal cancer(CRC).Methods: After computed tomography(CT) radiomics features extraction, a radiomics signature was constructed in derivation cohort(346 CRC patients). A prediction model was developed to integrate the radiomics signature and clinical candidate predictors [age, sex, tumor location, and carcinoembryonic antigen(CEA) level]. Apparent prediction performance was assessed. After internal validation, independent temporal validation(separate from the cohort used to build the model) was then conducted in 217 CRC patients. The final model was converted to an easy-to-use nomogram.Results: The developed radiomics nomogram that integrated the radiomics signature and CEA level showed good calibration and discrimination performance [Harrell's concordance index(c-index): 0.817; 95% confidence interval(95% CI): 0.811–0.823]. Application of the nomogram in validation cohort gave a comparable calibration and discrimination(c-index: 0.803; 95% CI: 0.794–0.812).Conclusions: Integrating the radiomics signature and CEA level into a radiomics prediction model enables easy and effective risk assessment of PNI in CRC. This stratification of patients according to their PNI status may provide a basis for individualized auxiliary treatment. | Yanqi Huang Lan He Di Dong Caiyun Yang Cuishan Liang Xin Chen Zelan Ma Xiaomei Huang Su Yao Changhong Liang Jie Tian Zaiyi Liu | 2018 | Chinese Journal of Cancer Research2018，30，1： | 23 |
| 7 | Clinical epidemiology of gastric cancer in Hehuang valley of China:A 10-year epidemiological study of gastric cancer显示文摘AIM:To investigate the clinical epidemiological characteristics of gastric cancer in the Hehuang valley,China,to provide a reference for treatment and prevention of regional gastric cancer.METHODS:Between February 2003 and February2013,the records of 2419 patients with gastric cancer were included in this study.The patient’s characteristics,histological and pathological features,as well as the dietary habits of the patients,were investigated.RESULTS:The clinical data showed that adenocarcinoma was the leading histological type of gastric cancer in this area.Characteristics of gastric cancer in different ethnic groups and age showed that the 60.55-65.50years group showed the high incidence of gastric cancer in all ethnic groups.There were more male gastric cancer patients than female.Intestinal was the most common type of gastric cancer in the Hehuang valley.There was no significant difference in the proportion of sex in terms of Helicobacter pylori infection.The impact of dietary habits on gastric cancer showed that regular consumption of fried or grilled food,consumption of high-salt,high-fat and spicy food and drinking strong Boiled brick-tea were three important factors associated with gastric cancer in males and females.CONCLUSION:Differences existed in race,sex,and age of patients according to the epidemiology of gastric cancer in the Hehuang valley.Moreover,dietary habits was also an important factor contributing to gastric cancer. | Su Yan Bin Li Zhen-Zhong Bai Jun-Qi Wu Da-Wei Xie Ying-Cai Ma Xu-Xiang Ma Jun-Hui Zhao Xin-Jian Guo | 2014 | World Journal of Gastroenterology2014，20，30： | 22 |
| 8 | Preliminary study on proteomics of gastric carcinoma and its clinical significance显示文摘AIM: To explore the preliminary identification of serum protein pattern models that may be novel potential biomarkers in the detection of gastric cancer.METHODS: A total of 130 serum samples, including 70 from patients with gastric cancer and 60 from healthy adults, were detected by surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The data of spectra were analyzed by Biomarker Patterns Software (BPS). Thirty serum samples of gastric cancer patients and 30 serum samples of healthy adults were grouped into the training group to build models, and the other 70 samples were used to test and evaluate the models. The samples of the test group were judged only with their peaks'height and were separated into cancer group or healthy control group by BPS automatically and the judgments were checked with the histopathologic diagnosis of the samples.RESULTS: Sixteen mass peaks were found to be potential biomarkers with a significant level of P<0.01.Among them, nine mass peaks showed increased expression in patients with gastric cancer. Analyzed by BPS, two peaks were chosen to build the model for gastric cancer detection. The sensitivity, specificity, and accuracy of the model were 90%, 36/40, 86.7%, 26/30,and 88.6%, 62/70, respectively, which were greatly higher than those of clinically used serum biomarkers CEA (carcinoembryonic antigen), CA19-9 and CA72-4.Stage Ⅰ/Ⅱ gastric cancer samples of the test group were all judged correctly.CONCLUSION: The novel biomarkers in serum and the established model could be potentially used in the detection of gastric cancer. However, large-scale studies should be carried on to further explore the clinical impact on the model. | Hong-Gang Qian Jing-Shen Hong Ma Hua-Chong Ma Ya-Hui Su Chun-Yi Hao Bao-Cai Xing Xin-Fu Huang Cheng-Chao Shou | 2005 | World Journal of Gastroenterology2005，11，40： | 20 |
| 9 | Establishing eutrophication assessment standards for four lake regions, China显示文摘The trophic status assessment of lakes in diferent lake regions may provide important and fundamental information for lake trophic state classification and eutrophication control. In this study, a region-specific lake eutrophication assessment standard was established through a frequency distribution method based on chlorophyll-a concentration. The assessment standards under the oligotrophic state for lakes in the Eastern plain, Yungui Plateau, Northeast Plain and Mountain Mongolia-Xinjiang regions are total phosphorus of 0.068,0.005, 0.011, 0.005 mg/L; total nitrogen of 1.00, 0.16, 0.37, 0.60 mg/L; Secchi depth of 0.60, 8.00, 1.55, 3.00 m; and CODMn of2.24, 1.00, 5.11, 4.00 mg/L, respectively. Moreover, a region-specific comprehensive trophic level index was developed to provide an understandable assessment method for the public. The results indicated that the frequency distribution analysis based on chlorophyll-a combined with trophic level index provided a useful metric for the assessment of the lake trophic status. In addition, the diference of eutrophication assessment standards in diferent lake regions was analyzed, which suggested that the sensitivities of algae to nutrients and the assessment standard of trophic status possessed significant regional diferences for the four lake ecoregions. Lake eutrophication assessment standards would contribute to maximizing the efectiveness of future management strategies, to control and minimize lake eutrophication problems. | Shouliang Huo Chunzi Ma Beidou Xi Jing Su Fengyu Zan Danfeng Ji Zhuoshi He | 2013 | Journal of Environmental Sciences2013，25，10： | 18 |
| 10 | Expression of Yes-associated protein in non-small cell lung cancer and its relationship with clinical pathological factors显示文摘 | SU Li-li MA Wei-xia YUAN Jian-feng SHAO Yang XIAO Wei JIANG Shu-juan | 2012 | Chinese Medical Journal2012，，22： | 18 |
| 11 | First experimental demonstration of coherent beam combining of more than 100 beams显示文摘Coherent beam combining of 107 beams has been demonstrated for the first time to the best of our knowledge.When the system was in closed loop,the pattern in far-field was stable and the fringe contrast was>96%.The impact of the dynamic tilt error,the piston error,and power inconsistency was theoretically analyzed.Meanwhile,the distribution law of dynamic tilt error was estimated and the correlation of the tilt dithering of different axis was analyzed statistically.The ratio of power in the central lobe was^22.5%.The phase residue error in the closed loop was~λ∕22,which was evaluated by the root-mean-square error of the signal generated from the photoelectric detector. | HONGXIANG CHANG QI CHANG JIACHAO XI TIANYUE HOU RONGTAO SU PENGFEI MA JIAN WUCAN LI MAN JIANG YANXING MA PU ZHOU | 2020 | Photonics Research2020，8，12： | 17 |
| 12 | Vision,application scenarios,and key technology trends for 6G mobile communications显示文摘With the global commercialization of the fifth-generation(5G)network,many countries,including China,USA,European countries,Japan,and Korea,have started exploring 6G mobile communication network,following the tradition of“planning the next while commercializing one generation”.Currently,studies on 6G networks are at the infancy stage.Research on the vision and requirements for 6G is still ongoing,and the industry is yet to clarify the key enabling technologies for 6G.However,6G will certainly build on the success of 5G.Therefore,developing high-quality 5G networks and seamlessly integrating 5G with verticals are the priorities before 2030,when 6G is projected to be commercialized.Also,global 5G standards will keep evolving to better support vertical applications.As a milestone,the Third-Generation Partnership Project(3GPP)published Release 16 in July 2020,which continuously enhanced the capabilities of mobile broadband service based on Release 15 and realized the support for low-delay and high-reliability applications,such as Internet of Vehicles and industrial Internet.Currently,3GPP is working on Releases 17 and 18,focusing on meeting the demands of medium-and high-data-rate machine communication with low-cost and high-precision positioning,which will be published in June 2022.Thus,6G networks will further expand the application fields and scope of the Internet of Things to accommodate those services and applications that are beyond the capabilities of 5G networks.Herein,we present our vision,application scenarios,and key technological trends for 6G networks.Furthermore,we propose several future research opportunities in 6G networks with regard to industrialization and standardization. | Zhiqin WANG Ying DU Kejun WEI Kaifeng HAN Xiaoyan XU Guiming WEI Wen TONG Peiying ZHU Jianglei MA Jun WANG Guangjian WANG Xueqiang YAN Jiying XIANG He HUANG Ruyue LI Xinhui WANG Yingmin WANG Shaohui SUN Shiqiang SUO Qiubin GAO Xin SU | 2022 | Science China(Information Sciences)2022，65，5： | 17 |
| 13 | D2 dissection in laparoscopic and open gastrectomy for gastric cancer显示文摘AIM: To evaluate the radicalness and safety of laparoscopic D2 dissection for gastric cancer. METHODS: Clinicopathological data from 209 patients with gastric cancer, who underwent radical gastrectomy with D2 dissection between January 2007 and February 2011, were analyzed retrospectively. Among these patients, 131 patients underwent laparoscopyassisted gastrectomy (LAG) and 78 underwent open gastrectomy (OG). The parameters analyzed included operative time, blood loss, blood transfusion, morbidity, mortality, the number of harvested lymph nodes (HLNs), and pathological stage.RESULTS: There were no significant differences in sex, age, types of radical resection [radical proximal gastrectomy (PG + D2), radical distal gastrectomy (DG + D2) and radical total gastrectomy (TG + D2)], and stages between the LAG and OG groups (P > 0.05). Among the two groups, 127 cases (96.9%) and 76 cases (97.4%) had 15 or more HLNs, respectively. The average number of HLNs was 26.1 ± 11.4 in the LAG group and 24.2 ± 9.3 in the OG group (P = 0.233). In the same type of radical resection, there were no signifi cant differences in the number of HLNs between the two groups (PG + D2: 21.7 ± 7.5 vs 22.4 ± 9.3; DG + D2: 25.7 ± 11.0 vs 22.3 ± 7.9; TG + D2: 30.9 ± 13.4 vs 29.3 ± 10.4; P > 0.05 for all comparisons). Tumor free margins were obtained in all cases. Compared with OG group, the LAG group had signifi cantly less blood loss, but a longer operation time (P < 0.001). The morbidity of the LAG group was 9.9%, which was not signifi cantly different from the OG group (7.7%) (P = 0.587). The mortality was zero in both groups. CONCLUSION: Laparoscopic D2 dissection is equivalent to OG in the number of HLNs, regardless of tumor location. Thus, this procedure can achieve the same radicalness as OG. | Ming Cui lia-Di Xing Yi-Yuan Ma Zhen-Dan Yao Nan Zhang Xiang-Qian Su Wei Yang | 2012 | World Journal of Gastroenterology2012，18，8： | 16 |
| 14 | Treatment and outcomes of 1041 pediatric patients with neuroblastoma who received multidisciplinary care in China显示文摘Importance:Neuroblastoma is the most common extracranial malignant solid tumor in children.Multidisciplinary care is critical to improving the survival of pediatric patients with neuroblastoma.Objective:To systematically summarize the clinical characteristics of children with neuroblastoma and evaluate their prognosis with multidisciplinary care provided in a single center.Methods:This retrospective study analyzed the clinical data of 1041 patients with neuroblastoma who were diagnosed,treated,and followed-up in the Hematology-Oncology Center of Beijing Children’s Hospital from 2007 to 2019.Results:The median age at diagnosis was 34 months;80.8%of the patients were younger than 5 years of age.Notably,243 patients(23.3%)were classified as low-risk,249 patients(23.9%)were classified as intermediate-risk,and 549(52.7%)were classified as high-risk.Furthermore,956 patients underwent surgical resections;986(94.7%)patients received chemotherapy;and 176 patients with high-risk neuroblastoma received hematopoietic stem cell transplantation.The 5-year event-free survival(EFS)rate was 91.3%and 5-year overall survival(OS)rate was 97.5%in low-risk group;in the intermediate-risk group,these rates were 85.1%and 96.7%,respectively,while they were 37.7%and 48.9%in the high-risk group(P<0.001 for both).The 5-year EFS and OS rates were significantly higher in patients diagnosed between 2015 and 2019 than in patients diagnosed between 2007 and 2014(P<0.001).In total,278 patients(26.7%)exhibited tumor relapse or progression;the median interval until relapse or progression was 14 months.Of the 233 patients who died,83%died of relapse or progression of neuroblastoma and 4.3%died of therapy-related complications.Interpretation:The 5-year OS rate was low in high-risk patients,compared with low-and intermediate-risk patients.Multidisciplinary care is critical for improvement of survival in pediatric patients with neuroblastoma.Additional treatment strategies should be sought to improve the prognosis of patients with high-risk neuroblastoma. | Yan Su Hong Qin Chenghao Chen Shengcai Wang Shihan Zhang Dawei Zhang Mei Jin Yun Peng Lejian He Xiaoman Wang Suyun Qian Maoquan Qin Ming Ge Fuquan Zhang Qi Zeng Huanmin Wang Xiaoli Ma Xin Ni | 2020 | Pediatric Investigation2020，4，3： | 16 |
| 15 | Development status of high power fiber lasers and their coherent beam combination显示文摘High-power fiber laser has been emerged great potential in a wide range of applications and becomes a robust candidate for high energy solid state laser system. To further increase the output brightness of single-channel fiber laser, high-brightness pump sources and high-power-handling passive components should be fabricated and utilized in the fiber laser systems, in addition to the advanced techniques for multiple nonlinear effects managements. The state-of-the-art high power fiber lasers are reviewed, in terms of narrow-linewidth fiber lasers, broadband fiber lasers and fiber lasers at 2 μm. Coherent beam combining is a promising technique to obtain higher output power while maintaining excellent beam quality simultaneously,which breaks through the bottlenecks of single-channel fiber laser. Based on a series of key techniques for coherent beam combining, high-power coherent beam combining of fiber lasers could be enabled with high combining efficiency. In this paper, we review the progress of high-power fiber lasers and their coherent beam combining in the recent decade, particularly the relevant work in our group. The future prospects of fiber lasers and coherent beam combining technique are also discussed. | Zejin LIU Xiaoxi JIN Rongtao SU Pengfei MA Pu ZHOU | 2019 | Science China(Information Sciences)2019，62，4： | 15 |
| 16 | UAV Online Path Planning Algorithm in a Low Altitude Dangerous Environment显示文摘UAV online path-planning in a low altitude dangerous environment with dense obstacles, static threats(STs)and dynamic threats(DTs), is a complicated, dynamic, uncertain and real-time problem. We propose a novel method to solve the problem to get a feasible and safe path. Firstly STs are modeled based on intuitionistic fuzzy set(IFS) to express the uncertainties in STs. The methods for ST assessment and synthesizing are presented. A reachability set(RS) estimator of DT is developed based on rapidly-exploring random tree(RRT) to predict the threat of DT. Secondly a subgoal selector is proposed and integrated into the planning system to decrease the cost of planning, accelerate the path searching and reduce threats on a path. Receding horizon(RH) is introduced to solve the online path planning problem in a dynamic and partially unknown environment. A local path planner is constructed by improving dynamic domain rapidly-exploring random tree(DDRRT) to deal with complex obstacles. RRT* is embedded into the planner to optimize paths. The results of Monte Carlo simulation comparing the traditional methods prove that our algorithm behaves well on online path planning with high successful penetration probability. | Naifeng Wen Lingling Zhao Xiaohong Su Peijun Ma | 2015 | IEEE/CAA Journal of Automatica Sinica2015，2，2： | 15 |
| 17 | COVID-19: Challenges to GIS with Big Data显示文摘The outbreak of the 2019 novel coronavirus disease(COVID-19)has caused more than 100,000 people infected and thousands of deaths.Currently,the number of infections and deaths is still increasing rapidly.COVID-19 seriously threatens human health,production,life,social functioning and international relations.In the fight against COVID-19,Geographic Information Systems(GIS)and big data technologies have played an important role in many aspects,including the rapid aggregation of multi-source big data,rapid visualization of epidemic information,spatial tracking of confirmed cases,prediction of regional transmission,spatial segmentation of the epidemic risk and prevention level,balancing and management of the supply and demand of material resources,and socialemotional guidance and panic elimination,which provided solid spatial information support for decision-making,measures formulation,and effectiveness assessment of COVID-19 prevention and control.GIS has developed and matured relatively quickly and has a complete technological route for data preparation,platform construction,model construction,and map production.However,for the struggle against the widespread epidemic,the main challenge is finding strategies to adjust traditional technical methods and improve speed and accuracy of information provision for social management.At the data level,in the era of big data,data no longer come mainly from the government but are gathered from more diverse enterprises.As a result,the use of GIS faces difficulties in data acquisition and the integration of heterogeneous data,which requires governments,businesses,and academic institutions to jointly promote the formulation of relevant policies.At the technical level,spatial analysis methods for big data are in the ascendancy.Currently and for a long time in the future,the development of GIS should be strengthened to form a data-driven system for rapid knowledge acquisition,which signifies ts that GIS should be used to reinforce the social operation parameterization of models and methods,especially when providing support for social management. | Chenghu Zhou Fenzhen Su Tao Pei An Zhang Yunyan Du Bin Luo Zhidong Cao Juanle Wang Wen Yuan Yunqiang Zhu Ci Song Jie Chen Jun Xu Fujia Li Ting Ma Lili Jiang Fengqin Yan Jiawei Yi Yunfeng Hu Yilan Liao Han Xiao | 2020 | Geography and Sustainability2020，1，1： | 13 |
| 18 | PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix显示文摘Biofilms 是在细胞外的矩阵嵌入的微生物的联系表面的社区。Exopolysaccharide 是在维持 biofilm 建筑学并且保护居民 biofilm 细菌免受抗菌剂和主人免疫者攻击的伤害的细胞外的矩阵的一个批评部件。然而,指向矩阵 exopolysaccharides 的自我生产的因素,仍然糟糕被理解。这里,我们显示出那 PslG,在 Pseudomonas aeruginosa 涉及关键 biofilm 矩阵 exopolysaccharide Psl 的合成的蛋白质,阻止 biofilm 形成并且拆卸存在在外长地供应的在 nanomolar 集中的分钟以内的 biofilms。PslG 的水晶结构显示 endoglycosidase 的典型特征。PslG 主要破坏 Psl 矩阵从 biofilms 驱散细菌。PslG 处理显著地提高 biofilm 敏感到抗菌素和巨噬细胞房间,导致在一只老鼠的改进 biofilm 清理植入感染模型。而且, PslG 对大量 Pseudomonas 种类显示出 biofilm 抑制和拆卸活动,显示它在与 biofilm 相关的复杂并发症作斗争的大潜力。 | Shan Yu Tiantian Su Huijun Wu Shiheng Liu Di Wang Tianhu Zhao Zengjun Jin Wenbin Du Mei-Jun Zhu Song Lin Chua Liang Yang Deyu Zhu Lichuan Gu Luyan Z Ma | 2015 | Cell Research2015，25，12： | 13 |
| 19 | CRISPR/Cas9-mediated PINK1 deletion leads to neurodegeneration in rhesus monkeys显示文摘Dear Editor,PINK1 mutations cause autosomal recessive and early-onset Parkinson’s disease(PD)with selective neurodegeneration.Unfortunately,current PINK1 knockout(KO)mouse1,2 and pig models3,4 are unable to recapitulate the selective and overt neurodegeneration seen in PD patients.Furthermore,endogenous Pink1 in the mouse brain is expressed at very low levels and can only be detected via immunoprecipitation,5 meaning that PINK1’s function in the mammalian brain needs to be assessed using larger animals that are closer to humans.We previously used CRISPR/Cas9 to target the monkey gene in one-cell stage embryos.6 Using the same approach,we designed two gRNAs to target exon 2(T1)and exon 4(T2),which encode a kinase domain in the PINK1 gene of rhesus monkeys(Fig.1a).CRISPR/Cas9 and PINK1 gRNAs were injected into one-cell stage rhesus monkey embryos.A T7E1 assay and sequencing of PCR products from the injected embryos showed high efficiency(61.5%)in targeting PINK1(Fig.1b and Supplementary information,Fig.S1).Transfer of 87 embryos to 28 surrogate rhesus monkeys resulted in 11 pregnancies(39.2%)(Fig.1b).Eleven fetuses developed to term and were born naturally.Of these live monkeys,eight carried PINK1 mutations(M),and three were wild type(WT).However,three mutant monkeys(M1,M3 and M4)were newborn triplets that struggled to survive and died 3-4 days after birth.One WT newborn monkey also died after a difficult labor.Another mutant monkey(M2)died 7 days after birth without noticeable warning signs or symptoms.The other three mutant monkeys(M6,M7 and M8)have lived for three years;M5,however,reduced its food intake and showed weakness at the age of 1.5 years,and died 30 days after anesthesia for MRI examination. | Weili Yang Yunbo Liu Zhuchi Tu Chong Xiao Sen Yan Xishan Ma Xiangyu Guo Xiusheng Chen Peng Yin Zhengyi Yang Su Yang Tianzi Jiang Shihua Li Chuan Qin Xiao-Jiang Li | 2019 | Cell Research2019，29，4： | 12 |
| 20 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
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