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| 1 | Detection and characterization of murine colitis and carcinogenesis by molecularly targeted contrast-enhanced ultrasound显示文摘AIM To study mucosal addressin cellular adhesion molecule-1(MAd CAM-1) and vascular endothelial growth factor(VEGF)-targeted contrast enhanced ultrasound(CEUS) for the assessment of murine colitis and carcinogenesis. METHODS C57BL/6 mice were challenged with 3% dextran sodium-sulfate(DSS) for three, six or nine days to study the development of acute colitis. Ultrasound was performed with and without the addition of unspecific contrast agents. MAd CAM-1-targeted contrast agent was used to detect and quantify MAd CAM-1 expression. Inflammatory driven colorectal azoxymethane(AOM)/DSS-induced carcinogenesis was examined on day 42 and 84 using VEGF-targeted contrast agent. Highly specific tissue echogenicity was quantified using specialized software. Sonographic findings were correlated to tissue staining, western blot analysis and immunohistochemistry to quantify the degree of inflammation and stage of carcinogenesis. RESULTS Native ultrasound detected increased general bowel wall thickening that correlated with more progressed and more severe DSS-colitis(healthy mice: 0.3 mm ± 0.03 vs six days DSS: 0.5 mm ± 0.2 vs nine days DSS: 0.6 mm ± 0.2, P < 0.05). Moreover, these sonographic findings correlated well with clinical parameters such as weight loss(r2 = 0.74) and histological damage(r2 = 0.86)(P < 0.01). In acute DSS-induced murine colitis, CEUS targeted against MAd CAM-1 detected and differentiated stages of mild, moderate and severe colitis via calculation of mean pixel contrast intensity in decibel(9.6 d B ± 1.6 vs 12.9 d B ± 1.4 vs 18 d B ± 3.33, P < 0.05). Employing the AOM/DSSinduced carcinogenesis model, tumor development was monitored by CEUS targeted against VEGF and detected a significantly increased echogenicity in tumors as compared to adjacent healthy mucosa(healthy mucosa, 1.6 d B ± 1.4 vs 42 d, 18.2 d B ± 3.3 vs 84 d, 18.6 d B ± 4.9, P < 0.01). Tissue echogenicity strongly correlated with histological analysis and immunohistochemistry findings(VEGF-positive cells in 10 high power fields of healthy mucosa: 1 ± 1.2 vs 42 d after DSS start: 2.4 ± 1.6 vs 84 d after DSS start: 3.5 ± 1.3, P < 0.01). CONCLUSION Molecularly targeted CEUS is a highly specific and noninvasive imaging modality, which characterizes murine intestinal inflammation and carcinogenesis in vivo. | Markus Brückner Jan Heidemann Tobias M Nowacki Friederike Cordes Jorg Stypmann Philipp Lenz Faekah Gohar Andreas Lügering Dominik Bettenworth | 2017 | World Journal of Gastroenterology2017，23，16： | 4 |
| 2 | Prognostic value of tis-sue Doppler imaging in patients with chronic congestive heart failure显示文摘 | Acil T Wichter T Stypmann J | 2005 | Intern J Cadiol2005，103，： | 1 |
| 3 | Partial left ventriculectomy in modified adriamycin-induced cardiomyopathy in the dog显示文摘 | Christiansen S Stypmann JJ Jahn UR | 2003 | J Heart Lung Transplant2003，22，： | 1 |
| 4 | Partial left ventriculectomy in modified adriamycin-induced cardiomyopathy in the dog显示文摘 | Christiansen S Stypmann JJ Jahn UR | 2003 | J Heart Lung Transplant2003，22，： | 1 |
| 5 | Contrast transeranial doppler ultrasound in the detection of right to left shunts: Time window and threshold in microbubble numbers显示文摘 | Droste DW Silling K Stypmann J | 2000 | Stroke2000，31，： | 1 |
| 6 | Naloxone improves functional recovery of myocardial stunning in conscious dogs through its action on the central nervous system 显示文摘 | Weber TP Stypmann J Meissner A | 2001 | Br J Anaesth2001，86，4： | 1 |
| 7 | Contrast transcranial Doppler ultrasound in the detection of right-to-left shunts: compar/son of different procedures and different contrast agents 显示文摘 | Droste DW Krlete JU Stypmann J | 1999 | Stroke1999，30，： | 1 |
| 8 | Doppler echocardiography and tissue Doppler imaging in the healthy rabbit: difference of cardiac function during awake and anaesthetized examination显示文摘 | Stypmann J Engelen MA Breithardt AK | 2007 | Int J Cardiol2007，115，： | 1 |
| 9 | Atorvastatin therapy is associated with reduced levels of N-terminal prohormone brain natriuretic peptide and improved cardiac function in patients with heart failure 显示文摘 | Stypmann J Schubert A Welp H | 2008 | Clin Cardiol2008，31，10： | 1 |
| 10 | Contrast transcranial doppler ultrasound in the detection of right-to-left shunts : time window and threshold in microbubble numbers 显示文摘 | Droste DW Silling K Stypmann J | 2000 | Stroke2000，31，： | 1 |
| 11 | Reduced repolarization re- serve due to anthraeyeline therapy facilitates torsade de pointes induced by IKr blockers 显示文摘 | Milberg P Fleiseher D Stypmann J | 2007 | Basic Res Cardiol2007，102，1： | 1 |
| 12 | Reduced repolarization reserve due to anthracucline therapy facilitates torsade de pointes induced by IKr blockers显示文摘 | Milberg P Fleischer D Stypmann J | 2007 | Basic Res Cardiol2007，102，1： | 1 |
| 13 | Prognostic value of tissue Doppler imaging in patients with chronic congestive heart failure显示文摘 | Acil T Wichter T Stypmann J | 2005 | Int J Cardiol2005，103，2： | 1 |
| 14 | Dynamic regulation of MEK/Erks and Akt/GSK-3beta in human end-stage heart failure af- ter left ventricular mechanical support: myocardial mechanotransduc- tion-sensitivity as a possible molecular mechanism 显示文摘 | Baba HA Stypmann J Grabellus F | 2003 | Cardiovasc Res2003，59，2： | 1 |
| 15 | Dilated cardiomyopathy in mice deficient for the lysosomal cysteine peptidase cathepsin L显示文摘 | Stypmann J Glaser K Roth W | 2002 | PNAS2002，99，9： | 1 |
| 16 | Reversible pulmonary hy- pertension in heart transplant candidates: to transplant or not to trans- plant 显示文摘 | Klotz S Wenzelburger F Stypmann J | 2006 | Ann Thorac Surg2006，82，： | 1 |
| 17 | Dynamic regulation of MEK/Erks and Akt/GSK-3beta in human end-stage heart failure after left ventricular mechanical support: myocardial mechanotransduction -sensitivity as a possible molecular mechanism 显示文摘 | Baba HA Stypmann J Grabellus F | 2003 | Cardiovasc Res2003，59，2： | 1 |
| 18 | Left ventricular pressure and volume unloading during pulsatile versus nonpulsatile left ventricular assist device support显示文摘 | Klotz S Deng MC Stypmann J | 2004 | Ann Thorac Surg2004，77，1： | 1 |
| 19 | Cardiac and ocular pathologies in a mouse model of mueopolysaecharidosis typeVI 显示文摘 | Straueh OF Stypmann J Reinheekel T | 2003 | Pediatr Res2003，54，5： | 1 |
| 20 | Naloxone improves functional recovery of myocardial stunning in conscious dogs through its action on the central nervous system显示文摘 | Weber TP Stypmann J Meibner M | 2001 | Br J Anaesth2001，86，4： | 1 |
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