|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Increasing the frequency of CIK cells adoptive immunotherapy may decrease risk of death in gastric cancer patients显示文摘AIM: To analyze the correlation between cytokineinduced killer (CIK) cells adoptive immunotherapy and cancer-related death in gastric cancer patients. METHODS: One hundred and fifty-six gastric cancer patients after operation at the Third Affiliated Hospital of Soochow University were enrolled in this study. Their clinical data including demographic characteristics, operation time, tumor size, pathological type and staging, tumor metastasis, outcome of chemotherapy or CIK cells adoptive immunotherapy, survival time or time of death were collected with a standard structured questionnaire. Kaplan-Meier method was used to estimate the median survival time, and the 2- and 5- year survival rates. Hazard risk (HR) and 95% confidence interval (95% CI) of CIK cells adoptive immunotherapy for gastric cancer were calculated using the two-stage time-dependent covariates Cox model. RESULTS: The survival time of gastric cancer patients was longer after CIK cells adoptive immunotherapy than after chemotherapy (χ 2 = 10.907, P = 0.001). The median survival time of gastric cancer patients was also longer after CIK cells adoptive immunotherapy than after chemotherapy (49 mo vs 27 mo, P < 0.05). The 2- and 5-year survival rates of gastric cancer patients were significantly higher after CIK cells adoptive immunotherapy than after chemotherapy (73.5% vs 52.6%, 40.4% vs 23.9%, P < 0.05). A significant difference was observed in the survival curve for patients who received CIK cells adoptive immunotherapy (0, 1-10, 11-25, and over 25 frequencies) (χ 2 = 14.534, P = 0.002). The frequencies of CIK cells adoptive immunotherapy were significantly related with the decreasing risk of death in gastric cancer patients after adjustment for sex and age of the patients, tumor stage and relapse (HR = 0.54, 95% CI: 0.36-0.80) when the first stage Cox model was used to define the subjects who remained alive beyond 36 mo as survivors. However, no correlation was observed between the frequencies of death in CIK cells adoptive immunotherapy and the risk of gastric cancer patients (HR = 1.09, 95% CI: 0.63-0.89) when the second stage Cox model was used to define the subjects who survived for more than 36 mo as survivors. CONCLUSION: The survival time of the gastric cancer patients treated with chemotherapy combined with CIK cells adoptive immunotherapy is significantly longer than that of the patients treated with chemotherapy alone and increasing the frequency of CIK cells adoptive immunotherapy seems to benefit patients more. | Jing-Ting Jiang, Chang-Ping Wu, Lu-Jun Chen, Xiao Zheng, Department of Tumor Biological Treatment, Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu Province, China Yi-Bei Zhu, Jing Sun, Xue-Guang Zhang, Key Laboratory of Stem Cell of Jiangsu Province, Institute of Biotechnology, Key Laboratory of Clinical Immunology of Jiangsu Province, Soochow University, Suzhou 215123, Jiangsu Province, China Yue-Ping Shen, Wen-Xiang Wei, Department of Medicine, Soochow University, Suzhou 215123, Jiangsu Province, China Bin-Feng Lu, Department of Immunology, University of Pitts- burgh School of Medicine, Pittsburgh, PA 15261, United States | 2010 | World Journal of Gastroenterology2010，16，48： | 82 |
| 2 | Full-thickness tissue engineered skin constructed with autogenic bone marrow mesenchymal stem cells显示文摘To explore the feasibility of repairing clinical cutaneous deficiency, autogenic bone marrow mesen-chymal stem cells (BMSCs) were isolated and differentiated into epidermal cells and fibroblasts in vitro supplemented with different inducing factors and biomaterials to construct functional tissue- engineered skin. The results showed that after 72 h induction, BMSCs displayed morphologic changes such as typical epidermal cell arrangement, from spindle shape to round or oval; tonofibrils, melano-somes and keratohyaline granules were observed under a transmission electronic microscope. The differentiated cells expressed epidermal stem cell surface marker CK19 (59.66% ± 4.2%) and epidermal cells differentiation marker CK10. In addition, the induced epidermal cells acquired the anti-radiation capacity featured by lowered apoptosis following exposure to UVB. On the other hand, the collagen microfibrils deposition was noticed under a transmission electronic microscope after differentiating into dermis fibroblasts; RT-PCR identified collagen type I mRNA expression in differentiated cells; radioimmunoassay detected the secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8) (up to 115.06 pg/mL and 0.84 ng/mL, respectively). Further in vivo implanting BMSCs with scaffold material short-ened skin wound repair significantly. In one word, autogenic BMSCs have the potential to differentiate into epidermal cells and fibroblasts in vitro, and show clinical feasibility acting as epidermis-like and dermis-like seed cells in skin engineering. | HE LiJuan, NAN Xue, WANG YunFang, GUAN LiDong, BAI CiXian, SHI ShuangShuang, YUAN HongFeng, CHEN Lin, LIU DaQing & PEI XueTao Laboratory of Stem Cells and Regenerative Medicine, Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing 100850, China | 2007 | Science China(Life Sciences)2007，50，4： | 16 |
| 3 | 国外STEM教师的培养培训及启示显示文摘STEM教育正在中国大地蓬勃兴起,许多地方和学校积极投身其中。在STEM教育实践探索过程中,能够胜任STEM教育的能力成为普遍议论的话题。开展STEM教育需要教师具备怎样的能力?由哪些能力构成?如何有效提升教师STEM教育的能力? | stem教师能力等级标准研制课题组 王素 李正福 | 2018 | 中国民族教育2018，，7： | 8 |
| 4 | Magnetic resonance evaluation of transplanted mesenchymal stem cells after myocardial infarction in swine显示文摘Objectives To trace and evaluate intracoronary transplanted mesenchymal stem cells(MSCs) labeled with superparamagnetic iron oxide(SPIO) by using magnetic resonance imaging(MRI) in a swine model of myocardial infarction (MI).Methods MSCs were transfected with a lentiviral vector carrying the gene encoding green fluorescent protein (GFP) and labeled in vitro with SPIO.Two weeks after MI, swine were randomized to intracoronary transplantation of dual -labeled MSCs(n = 10),MSCs-GFP(n = 10) and saline(n = 5).MRI examination was performed with a 1.5T clinical scanner at 24 hours,3 weeks and 8 weeks after cells transplantation. Signal intensity(SI) changes,cardiac function and MI size were measured using MRI.Correlation between MR findings and histomorphologic findings was also investigated. Results The labeling efficiency at a combination of 25μg Fe/ml SPIO and 0.8 pi/ml Lipofectamine 2000 reached 100%.SPIO labeling did not affect GFP fluorescence and dual-labeling did not affect cell proliferation(P>0.05). Multipotentiality was not affected especially for cardiomyocyte-like cells differentiation.Cardiac cell marker of a-MHC and actinin were positively expressed by immunofluorescence staining after induction.SI on T2 * WI decreased substantial- ly in the interventricular septum 24 hours after injection of MSCs.The intensity of hypo-intense signals appeared to increase throughout the later time points.Changes in SI at 24 hours,3 weeks and 8 weeks were 52.98%±10.74%,21.53%±5.40%and 6.23%±2.01%,respectively(P<0.01).DE-MRI demonstrated both dual-labeled MSCSs and MSCs-GFP could dramatically reduce the size of MI and improve cardiac function. Histological data revealed that prussian blue stain-positive cells were found mainly in the border zone which also showed green fluorescence but negative for macrophage marker(CD68).Gross pathologic examination revealed that engrafted MSCs dramatically reduce the extent of necrotic myocardium and promote the regeneration of new,contractile myocardium along the subendocardial surface of the MI. Conclusions MSCs could be efficiently and safely labeled with SPIO and GFP,and could be detected reproducibly and noninvasively in vivo using cardiac MRI.Intracoronary transplantation of dual-labeled MSCs could increase cardiac function and reduce the size of MI. | KE Yang~1,PENG Chao-quan~1,XIANG Peng~1,ZHANG Cheng-xi~1, ZOU Li-yuan~1,WU Xiao~1,YA Gao~1,KANG Z huang~2,HE Ke-ke~2,LIU Jin-lai~1 (1.Department of Cardiology,The Third Affiliated Hospital, Sun Yat-sen University,Guangzhou 2.Department of Radiology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 5100630 3.Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University,Guangzhou 5100120) | 2011 | 岭南心血管病杂志2011，17，S1： | 7 |
| 5 | On the“plasticity”of adult stem cells and its application in regenerative medicine显示文摘In recent years, with the increasingly further studies on embryonic stem cells and the recognition of the biologic characteristics of adult stem cells, it has been discovered that adult stem cells have another phenomenon of 'Plasticity' in addition to the characteristics of strong poten- tial for self-renewal, proliferation and multi-differentiation, which brings us the hope for regenerative medicine —— renewing new organ or tissue cells to replace those damaged by injury or diseases. Although the mechanism of 'Plastic- ity' and its application in the regenerative medicine are still in doubt, thorough exploration in these subjects would open up broad prospects for the use in cell and tissue engineering in the near future. | WANG Yunfang & PEI Xuetao Department of Stem Cell Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China Correspondence should be addressed to Pei Xuetao (e-mail: peixt@ nic.bmi.ac.cn) | 2003 | Chinese Science Bulletin2003，48，13： | 3 |
| 6 | Ion imprinted cryogel-based supermacroporous traps for selective separation of cerium(Ⅲ) in real samples显示文摘This study demonstrates the preparation and characterization of a novel ion imprinted cryogel which exhibits high affinity and selectivity towards Ce(Ⅲ) ions in aqueous solutions and bastnasite ore samples.2-Hydroxyethyl methacrylate(HEMA) and N-methacryloylamido antipyrine(MAAP) were used as functional monomers for the preparation of Ce(Ⅲ) imprinted cryogel. The effects of various factors such as initial Ce(Ⅲ) concentration, flow rate, pH, interaction time and ionic strength on the Ce(Ⅲ) binding to the prepared ion imprinted cryogels were also studied. The binding equilibrium for Ce(Ⅲ) is obtained in30 min at the flow rate of 0.5 mL/min. The maximum binding capacity of the prepared ion imprinted cryogel towards Ce(Ⅲ) is obtained as 36.58 mg/g at optimum conditions. The selectivity of the prepared ion imprinted cryogel towards Ce(Ⅲ) in the presence of other possible interfering lanthanide ions such as La(Ⅲ) and Nd(Ⅲ) were also performed. The obtained results showed that the prepared ion imprinted cryogel exhibits high selectivity and sensitivity towards Ce(Ⅲ) ions. The limit of detection(LOD) was found as 50 μg/L. | Rüstem Kecili brahim Dolak Berrin Ziyadanogullari Arzu Ersoz Ridvan Say | 2018 | Journal of Rare Earths2018，36，8： | 2 |
| 7 | Treatment and prognosis of rectal prolapse in cystic fibrosis 显示文摘 | Stem RC | 1982 | Gastroenterology1982，82，4： | 1 |
| 8 | Cecal carriage of Clostri-dinm perfringens in broiler chickens given mucosal starter culture 显示文摘 | Craven S E Stem N J Cox N A | 1999 | Avian Diseases1999，43，： | 1 |
| 9 | Promoter hypermethylation of tumor suppressor genes in urine from patients with cervical neoplasia显示文摘 | Feng Q Hawes SE Stem JE | 2007 | Cancer Epidemiol Biomarkers Prey2007，16，： | 1 |
| 10 | Model estimates of the frequency of white and near white segregants in the American Negro显示文摘 | | 1953 | Acta Genet1953，4，： | 1 |
| 11 | General retail store patronage loyalty as a determinant of consumer outshopping behavior显示文摘 | Hozier G C and Stem D E | 1985 | Journal of the Academy of Marketing Science1985，13，1： | 1 |
| 12 | Untangling the Ori- gins of Competitive Advantage显示文摘 | Cockbum A M Henderson R M Stem S | 2000 | Strategic Management Journal2000，，21： | 1 |
| 13 | Immunohistochemical reaction to human milk fat globule antibodies and breast cancer differentiation 显示文摘 | Stems EE Fletcher WA Zee B | 1992 | Breast Cancer Res Treat1992，23，1： | 1 |
| 14 | A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants 显示文摘 | Calsamiglia S Stem M D | 1995 | J Anim Sci1995，73，： | 1 |
| 15 | The association of bone mineral density with vitamin D receptor gene polymorphisms显示文摘 | Gong G Stem HS Cheng SC | 1999 | Osteoporos Int1999，9，： | 1 |
| 16 | The homeostasis model in the San Autonio Heart Study 显示文摘 | Haffner SM Mietfinen H Stem MP | 1997 | Diabetes Care1997，20，7： | 1 |
| 17 | Relationship between intimamedia thickness in the common carotid artery and atherosclerosis in the carotid bifurcation显示文摘 | Rosfors S Stem SH Rerstin JU | 1998 | Stroke1998，29，7： | 1 |
| 18 | XPD codon 751 polymorphism,metabolism genes,smoking,and bladder cancer risk显示文摘 | Stem MC Johnson LR Bell DA | 2002 | Cancer Epidemiol Biomark Prev2002，11，101： | 1 |
| 19 | The homeostasis model in the San Antonin Heart stndy显示文摘 | Haffner SM Miettinen H Stem MP | 1997 | Diabetes Care1997，20，： | 1 |
| 20 | Isolation of mumps vires from theinner ear after sudden deafness显示文摘 | Westmore GA Pickard BH Stem H | 1979 | Br Med J1979，1，6155： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员