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| 1 | 从白屈菜乙醇提取物中分离出的白屈菜碱通过p38-p53和PI3K/AKT信号转导通路促进 HeLa细胞凋亡(英文)显示文摘目的:研究从白屈菜乙醇提取物中分离出的白屈菜碱在诱导 HeLa细胞凋亡中的作用及参与其作用的主要信号转导通路。方法:细胞先以不同浓度白屈菜碱处理48h,用噻唑蓝法分析确定半数致死量(median lethal dose,LD50)。用4′,6-二脒基-2-苯基吲哚染色,追踪分析核浓染以及 DNA 损伤和碎片的形态学变化,并用流式细胞术分析检测活性氧(reactive oxygen species,ROS)的产生以及细胞周期阻滞和线粒体膜电位的变化。用圆二色光谱分析寻找白屈菜碱和小牛胸腺 DNA可能的相互作用。用逆转录聚合酶链反应和蛋白免疫印迹法测定p38、p53、蛋白激酶B(protein kinase B,AKT)、磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinases,PI3K)、Janus激酶3(Janus kinase 3,JAK3)、信号转导及转录激活因子3(signal transducer and activator oftranscription 3,STAT3)等的mRNA和蛋白表达,以及E6、E7癌基因和促凋亡基因、抗凋亡基因的mRNA和蛋白表达。结果:根据白屈菜碱的LD50(30μg/mL),选定3种实验剂量,即22.5、30和37.5μg/mL。结果显示,白屈菜碱抑制了 HeLa细胞增殖,诱发其细胞凋亡,表现为 ROS的产生,细胞亚 G1和 G0/G1周期阻滞,线粒体膜电位变化和 DNA 碎片产生。圆二色光谱分析结果显示白屈菜碱和小牛胸腺 DNA 间存在有效的相互作用。信号通路的研究显示白屈菜碱通过上调p38、p53和其他促凋亡基因的表达,以及下调 AKT、PI3K、JAK3、STAT3、E6、E7和其他抗凋亡基因的表达,有效诱发细胞凋亡。结论:从白屈菜中分离出的白屈菜碱能通过改变p38-p53及 AKT/PI3激酶信号转导通路有效地诱发 HeLa细胞凋亡。 | Avijit Paul Kausik Bishayee Samrat Ghosh Avinaba Mukherjee Sourav Sikdar Debrup Chakraborty Naoual Boujedaini Anisur Rahman Khuda-Bukhsh | 2012 | 中西医结合学报2012，10，9： | 10 |
| 2 | Homeopathic mother tincture of Phytolacca decandra induces apoptosis in skin melanoma cells by activating caspase-mediated signaling via reactive oxygen species elevation显示文摘OBJECTIVE: Preventive measures against skin melanoma like chemotherapy are useful but suffer from chronic side effects and drug resistance. Ethanolic extract of Phytolacca decandra (PD), used in homeopathy for the treatment of various ailments like chronic rheumatism, regular conjunctivitis, psoriasis, and in some skin diseases was tested for its possible anticancer potential. METHODS: Cytotoxicity of the drug was tested by conducting 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on both normal (peripheral blood mononuclear cells) and A375 cells. Fluorescence microscopic study of 4',6-diamidino-2-phenylindole dihydrochloride-stained cells was conducted for DNA fragmentation assay, and changes in cellular morphology, if any, were also recorded. Lactate dehydrogenase activity assay was done to evaluate the percentages of apoptosis and necrosis. Reactive oxygen species (ROS) accumulation, if any, and expression study of apoptotic genes also were evaluated to pin-point the actual events of apoptosis. RESULTS: Results showed that PD administration caused a remarkable reduction in proliferation of A375 cells, without showing much cytotoxicity on peripheral blood mononuclear cells. Generation of ROS and DNA damage, which made the cancer cells prone to apoptosis, were found to be enhanced in PD-treated cells. These results were duly supported by the analytical data on expression of different cellular and nuclear proteins, as for example, by down-regulation of Akt and Bcl-2, up-regulation of p53, Bax and caspase 3, and an increase in number of cell deaths by apoptosis in A375 cells. CONCLUSION: Overall results demonstrate anticancer potentials of PD on A375 cells through activation of caspase-mediated signaling and ROS generation. | Samrat Ghosh Kausik Bishayee Avijit Paul Avinaba Mukherjee Sourav Sikdar Debrup Chakraborty Naoual Boujedaini Anisur Rahman Khuda-Bukhsh | 2013 | Journal of Integrative Medicine2013，11，2： | 5 |
| 3 | Molecular dynamics simulation of nanometer scale mechanical properties of hexagonal Mgsingle bondLi alloy显示文摘Nano mechanical behavior of Mgsingle bondLi nanowire is investigated under tension and compression to elicit property alteration due to Li alloying in Mg within hexagonal range.Embedded atom method(EAM)is employed to carry out present simulation work.Nanowire under consideration is supposed to be isotropic and mechanical behavior is uninfluenced by material texture.The elastic modulus,yield strength both in tension and compression is assessed with change in strain rate.Effects of temperature in tension and compression are studied.Results of present simulation work elicit serrated yielding under uniaxial tension,however,twin mediated deformation under compression is completely tuned with previously reported experimental works.This investigation bridges nanometer scale properties to microscale material response,which in turn can be applied for designing suitable robust processing routes of this material. | Avik Mahata Koushik Sikdar | 2016 | Journal of Magnesium and Alloys2016，4，1： | 3 |
| 4 | Matryoshka-caged gold nanorods: Synthesis, plasmonic properties, and catalytic activity显示文摘将 Matryoshka 关入笼中的金 nanorods (mCGNRs ) 被在一个调停种子的银涂层方法和流电的代替反应(GRR ) 之间轮流出现成功地综合。当 mCGNRs 的 matryoshka 层的数字增加了,拓宽并且是转移红的电浆子回声山峰,和向 4-nitrophenol (4-NTP ) 的减小的催化活动增加了。当有 6 层的 mCGNRs 在 4-nitrophenol 的减小被用作 nanocatalysts 时,反应率系数分别地比 gold-nanorod- 和 4-nitrophenol 的 caged-gold-nanorod-catalyzed 减小的高是 5.2-times 和 3.7-times。另外,光的 surface-plasmon-resonance-based 吸收提高了 mCGNRs 的催化表演。与聚氨酯泡沫的支持, mCGNRs 在这研究综合了能作为 recyclable 被使用为 4-nitrophenol 的减小的异构的催化剂。 | Wei Xiong Debabrata Sikdar Lim Wei Yap Pengzhen Guo Malin Premaratne Xinyong Li Wenlong Cheng | 2016 | Nano Research2016，9，2： | 3 |
| 5 | 北美香柏叶的乙醇提取物阻断A549细胞增殖并引起细胞凋亡的体外研究(英文)显示文摘目的:研究北美香柏叶的乙醇提取物对非小细胞肺癌A549细胞的抗肿瘤及抗增殖作用。方法:噻唑蓝法检验不同剂量北美香柏叶的乙醇提取物对细胞活性的影响。确定半数最大抑制浓度为282μg/mL,另外选择两个浓度188μg/mL和376μg/mL进行剂量依赖性检测。进行溴脱氧尿苷结合实验和细胞迁移实验检测药物的抗肿瘤细胞增殖活性。膜联蛋白V-异硫氰酸荧光黄-碘化丙啶双染色后采用荧光激活细胞分类分析仪对细胞早期凋亡进行检测。Hoechst 33258及吖啶橙-溴化乙啶荧光染色法进行DNA片段分析。间接酶联免疫吸附法分析Bax-Bcl2的调节和表达情况。逆转录聚合酶链反应检测caspase3基因表达情况,其活性和蛋白水平的表达则使用间接酶联免疫吸附法和蛋白印迹法进行检测。结果:A549的细胞活性在暴露于北美香柏叶的乙醇提取物24h后呈剂量依赖性下降。脱氧尿苷结合实验和细胞迁移实验表明细胞的增殖活性与暴露于药物的时间有时间依赖性关系。11.72%的细胞在双染色后呈阳性反应,表明药物引起了细胞的早期凋亡。药物作用24h后DNA片段彗星尾的出现以及Hoechst 33258荧光染色的增加提示显著的DNA缺口出现以及染色质凝聚。Bax的上调及Bcl2的下调表明了细胞凋亡的出现。逆转录聚合酶链反应、间接酶联免疫吸附法以及蛋白印迹法的检测结果表明caspase3的活性随着抗聚(腺苷二磷酸-核糖)聚合酶的表达的增加而增加。结论:北美香柏叶的乙醇提取物能够促进A549细胞凋亡并抑制其增殖活性。 | Avinaba Mukherjee Sourav Sikdar Kausik Bishayee Avijit Paul Samrat Ghosh Naoual Boujedaini Anisur Rahman Khuda-Bukhsh | 2012 | 中西医结合学报2012，10，12： | 2 |
| 6 | Evaluation of chemopreventive potentials of ethanolic extract of Ruta graveolens against A375 skin melanoma cells in vitro and induced skin cancer in mice in vivo显示文摘OBJECTIVE: Chemopreventive approach with natural products, particularly plants and plant-derived ones, is receiving increasing attention for their effective role against cancer without any palpable side effects. In this study, efficacy of ethanolic extract of Ruta graveolens(RG) on skin melanoma cells(A375) in vitro and on 7,12-dimethylbenz(a)anthracene(DMBA)-induced skin cancer in vivo has been tested in Swiss albino mice.METHODS: Studies on cell viability, apoptosis and autophagy induction were conducted in vitro. To check apoptosis, assays like alteration in mitochondrial membrane potential, annexin V-fluorescein isothiocyanate/propidium iodide assay and immunoblot were performed. Fluorescence microscopic and immunoblot assays were performed to confirm autophagy induction. The effects of RG were determined by evaluating body weight, tumor incidence, tumor volume and tumor burden in mice. Enzymatic and non-enzymatic antioxidant status was assessed. The role of some relevant signaling proteins was also analyzed.RESULTS: RG caused death of A375 cells through induction of caspase 3-mediated apoptosis and Beclin-1-associated autophagy. Moreover, RG administration(75 mg/kg body weight) which showed no acute or chronic toxicity, showed significant reduction in the skin tumor burden of DMBA-painted mice. RG also demonstrated potent anti-lipid peroxidative and antioxidant functions during the course of skin cancer induction by DMBA.CONCLUSION: Chemopreventive potential of RG was demonstrated from overall results of this study, indicating its possible use in therapeutic formulation of an effective drug to treat skin cancer. | Samrat Ghosh Sourav Sikdar Avinaba Mukherjee Anisur Rahman Khuda-Bukhsh | 2015 | Journal of Integrative Medicine2015，13，1： | 2 |
| 7 | Effect of graded dietary levels of aflatoxin on total serum proteins,albumin and globulins in broilers显示文摘 | BAKSHI C S SIKDAR A JOHRI T S | 1997 | Indian Journal of Comparative Microbiology Immunology and Infectious Diseases1997，18，2： | 1 |
| 8 | Plastid transformation in Arabidopsis thaliana 显示文摘 | Sikdar S R Serino G Chaudhuri S Pal M | 1998 | Plant Cell Rep1998，18，1： | 1 |
| 9 | Recovery of VOCs from surfactant solutions by pervaporation显示文摘 | JIANG J VANE L M SIKDAR S K | 1997 | Journal of Membrane Scinence1997，136，12： | 1 |
| 10 | Cytomorphological studies in triploid mulberry evolved by diploidization of female gamete cells 显示文摘 | Dwived N K Suryanarayana N Sikdar A K | 1989 | Cytologia1989，54，： | 1 |
| 11 | Optimization of muhicomponent pervaporation for removal of volatile organic compounds from water显示文摘 | Wenchang Ji A Hilaly Subhas K Sikdar | 1994 | J Membr Sci1994，97，： | 1 |
| 12 | Sustainable development and sustainability metrics 显示文摘 | Sikdar SK | 2003 | AIChE Journal2003，49，8： | 1 |
| 13 | -Gingerol isolatedfrom ginger attenuates sodium arsenite induced oxidative Stress andplays a corrective role in improving insulin signaling in mice 显示文摘 | Chakraborty D Mukherjee A Sikdar S | 2012 | Toxicology Letters2012，210，1： | 1 |
| 14 | Functional electrical stimula- tion of dorsiflexor muscle: effects on dorsiflexor strength, plan- tarflexor spasticity, and motor recovery in stroke patients 显示文摘 | Sabut SK Sikdar C Kumar R | 2011 | Neuro Rehab2011，29，4： | 1 |
| 15 | Loss of heterozygosity at APC and MCC genes of oral cancer and leukoplakia tissues from Indian tobacco chewers 显示文摘 | Sikdar N Paul R R Panda C K | 2003 | J Oral Pathol Med2003，32，8： | 1 |
| 16 | Kinetics of the decoloration of reactive dyes over visible light-irradiated TiO2 semiconductor photoeatalyst 显示文摘 | Chatterjee D Patnam V R Sikdar A | 2008 | J Hazard Mater2008，156，13： | 1 |
| 17 | On aggregating multiple indicators into a single metric for sustainability 显示文摘 | Sikdar SK | 2009 | Clean Technologies and Environmental Policy2009，11，： | 1 |
| 18 | Separation of azeotropic organic liquid mixtures by pervaporation显示文摘 | Dutta B K Sikdar S K | 1991 | AIChE Journal1991，37，4： | 1 |
| 19 | Simulation of Thermal History of Work Rolls of Finishing Stand in Hot Strip Mill: A Numerical Approach 显示文摘 | Sikdar S John S | 2006 | lronmaking and Steelmaking2006，33，6： | 1 |
| 20 | Effect ot pricing intervals on congestion-- sensitivity of network service prices显示文摘 | Yuksel M Kalyanaraman S Sikdar B | 1993 | Business and Ecomonics1993，28，1： | 1 |
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