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| 1 | A seed-specific AP2-domain transcription factor from soybean plays a certain role in regulation of seed ger-mination显示文摘Plant seed development and germination are under strict temporal and spatial regulation, and tran-scription factors play important roles in this regulation. In the present study we identified an EST ex-pressed specifically in the developing soybean seeds. The full length of the gene was obtained through further RACE analysis and the gene was named GmSGR. Sequence analysis revealed that this gene belonged to the AP2/ERF transcription factor family. Its AP2 domain had the highest similarity with that of the A-3 member AtABI4 of DREB subgroup in the AP2/ERF family in Arabidopsis. GmSGR did not exhibit transcriptional activation activity in the yeast assay system. GmSGR was overexpressed in Arabidopsis and the germination rates of the transgenic seeds were significantly higher than that of the wild type seeds under higher concentrations of ABA and glucose respectively. However, the germina-tion rates of the transgenic seeds were lower than that of control under salt stress. The expression of AtEm6 and AtRD29B was higher in the seedlings of the transgenic plants than that in the wild-type seedlings. These results suggest that GmSGR may confer reduced ABA sensitivity and enhanced salt sensitivity to the transgenic seeds through regulating the expression of AtEm6 and AtRD29B genes. | WANG ChunMei, WANG HuiWen, ZHANG JinSong & CHEN ShouYi National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Bei-jing 100101, China | 2008 | Science China(Life Sciences)2008，51，4： | 11 |
| 2 | Ectopic expression of soybean GmKNT1 in Arabidopsis results in altered leaf morphology and flower identity显示文摘Plant morphology is specified by leaves and flowers, and the shoot apical meristem (SAM) defines the architecture of plant leaves and flowers. Here, we reported the characterization of a soybean KNOX gene GmKNT1, which was highly homologous to Arabidopsis STM. The GmKNT1 was strongly expressed in roots, flowers and developing seeds. Its expression could be induced by IAA, ABA and JA, but inhibited by GA or cytokinin. Staining of the transgenic plants overexpressing GmKNT1-GUS fusion protein revealed that the GmKNT1 was mainly expressed at lobe region, SAM of young leaves, sepal and carpel, not in seed and mature leaves. Scanning electron micros-copy (SEM) disclosed multiple changes in morphology of the epidermal cells and stigma. The transgenic Arabidopsis plants overexpress-ing the GmKNT1 showed small and lobed leaves, shortened internodes and small clustered inflorescence. The lobed leaves might result from the function of the meristems located at the boundary of the leaf. Compared with wild type plants, transgenic plants had higher ex-pression of the SAM-related genes including the CUP, WUS, CUC1, KNAT2 and KNAT6. These results indicated that the GmKNT1 could affect multiple aspects of plant growth and development by regulation of downstream genes expression. | Jun Liu Da Ha Zongming Xie Chunmei Wang Huiwen Wang Wanke Zhang Jinsong Zhang Shouyi Chen | 2008 | Journal of Genetics and Genomics2008，35，7： | 8 |
| 3 | Molecular cloning, chromosomal mapping and expression analysis of disease resistance gene homologues in rice(Oryza sativa L.)显示文摘Resistance-like sequences have been amplified from first strand cDNA and genomic DNA of rice by PCR using oligonucleotide primers designed from sequence motifs conserved between resistance genes of tobacco and Arabidopsis thaliana. 3 PCR clones, designated Osr1, Osr2 and Osr3 which were 98% identical in nucleotide sequence level, have been found to be significantly homologous to known plant resistance genes and all contained the conserved motifs of NBS-LRR type resistance genes, such as P-loop, kinase2a, kinase3a and transmembrane domain. Southern hybridization revealed that rice resistance gene homologues were organized as a cluster in the genome. RFLP mapping using a DH population derived from an indica/japonica cross (Zhaiyeqing 8/Jingxi 17) and an RFLP linkage map assigned two copies of Osr1 and one copy of Osr3 to the distal position of chromosome 12 where a blast resistance QTL has been mapped previously. Northern blot analysis showed that Osr1 gene was constitutively transcribed in rice leaves, | Ziyin Li Shouyi Chen | 1999 | Chinese Science Bulletin1999，44，13： | 7 |
| 4 | Identification of salt-tolerance QTL in rice(Oryza sativa L.)显示文摘Quantitative trait loci (QTLs) controlling salt-tolerance at the seedling stage in rice (Oryza sativa L.) were identified by interval mapping (SIM) and composite interval mapping (CIM) using a doubled haploid population ZJDH and its high resolution genetic linkage map. The population was derived from an inter-subspecific cross between an indica variety Zhaiyeqing8 (ZYQ8) and a japonica variety Jingxi17 (JX17). Analysis of survival days of seedlings treated with 0.7% NaCl revealed that a major salt-tolerance quantitative trait locus (QTL), Std, was present between markers RG612 and C131 on chromosome 1 when using both MAPMAKER/QTL 1.1 and PLABQTL 1.0 (SIM).Its allele which contributes to salt-tolerance was from ZYQ8. In addition, seven more QTLs which give additive effect on salt-tolerance are identified when using PLABQTL(CIM), and most of them were from JX17. | Jiming Gong Ping He Qian Qian Lishuang Shen Lihuang Zhu Shouyi Chen | 1999 | Chinese Science Bulletin1999，44，1： | 7 |
| 5 | Hierarchical representation of on-chip context to reduce reconfiguration time and implementation area for coarse-grained reconfigurable architecture显示文摘In reconfigurable system,fast reconfiguration and small size of configuration contexts are strongly required to enhance the processing performance and reduce the implementation overhead.In this paper,a hierarchical representation of contexts for CGRA called HCC is proposed to satisfy the above requirements.In HCC,the contexts are constructed in a hierarchical fashion to thoroughly eliminate the repetitive portions of the contexts,not only reducing the overall contexts storage size,but also alleviating the contexts transportation overhead.The fast context-indexing mechanism is proposed in HCC to achieve high configuration speed,since the hierarchically organized contexts can be located and accessed conveniently.HCC has been verified in a reconfigurable processor called REMUS HP.Owing to HCC,when implementing H.264 decoding on REMUS HP,76.67%of the overall contexts are reduced compared with the traditional non-hierarchical one;and the configuration speed is averagely 23×increased compared with the latest reported optimized configuration mechanism on Virtex-4 FX60.REMUS HP is implemented on a 48.9 mm2silicon with TSMC 65 nm technology.Simulation shows that 1920×1088@30 fps could be achieved for H.264 high-profile decoding when exploiting a 200 MHz working frequency.Compared with the high performance version of XPP,the performance is 181%boosted. | WANG YanSheng LIU LeiBo YIN ShouYi ZHU Min CAO Peng YANG Jun WEI ShaoJun | 2013 | Science China(Information Sciences)2013，56，11： | 7 |
| 6 | An efficient VLSI architecture of speeded-up robust feature extraction for high resolution and high frame rate video显示文摘This paper proposes a VLSI architecture of the optimized Speeded-Up Robust Feature (SURF) algorithm. The SURF algorithm which is widely used in computer vision applications, locates interest points (IPoints) and extracts feature descriptors based on the surrounding gradients. In the proposed approach, the SURF algorithm is modified to make it more suitable for hardware implementation with little accuracy lost compared to OpenSURF, an open source software implementation based on OpenCV. The resource cost and throughput gain are balanced. Word Length Reduction (WLR) is adopted to compress the integral image and reduce the occupied memory resources. The orientations and the feature descriptors of the IPoints are calculated in a more efficient way, which significantly reduces memory accesses. Moreover, the operations are pipelined both in and among the proposed hardware modules. This VLSI architecture has been validated on FPGA (Xilinx Virtex-4 XC4VLX80), which is able to detect IPoints and extract SURF feature descriptors in a VGA (640 × 480) 64 fps video input with a 96 MHz working frequency while dissipating 1.278 W. This throughput is more than double of the ones reported in the latest literatures. | ZHANG WeiLong LIU LeiBo YIN ShouYi ZHOU RenYan CAI ShanShan WEI ShaoJun | 2013 | Science China(Information Sciences)2013，56，7： | 6 |
| 7 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 8 | Ethylene signaling in rice显示文摘Ethylene regulates many aspects of growth,development and responses to environmental stresses in plants.Its signaling pathway has been established in model dicotyledonous plant Arabidopsis.However,its roles and signal transduction in monocotyledous rice plant remain largely unknown.In this review,we summarize the current advances in rice ethylene signaling studies and compare these with the results from Arabidopsis and other plants.Most of the components homologous to those in Arabidopsis ethylene signaling pathway have been found in rice,including five ethylene receptors,OsEIN2,OsEIL1,and OsERFs.Rice ethylene receptors are functionally more divergent than that of Arabidopsis.OsEIN2 and OsEIL1 display limited roles in regulation of rice ethylene responses compared with their Arabidopsis orthologs.ERF-like proteins OsERF1 and OsEBP-89 appear to be involved in rice ethylene signaling.However,whether they are activated through OsEIN2 and OsEIL1-mediated pathway needs further studies.Given that rice uses ethylene to control many processes that do not exist in Arabidopsis,it seems that new components or new mechanisms may exist in rice ethylene signaling pathway. | MA Biao CHEN ShouYi ZHANG JinSong | 2010 | Chinese Science Bulletin2010，55，21： | 6 |
| 9 | The role of tocopherol cyclase in salt stress tolerance of rice(Oryza sativa)显示文摘Tocopherols synthesized exclusively by photosynthetic organisms are major antioxidants in biomembranes.In plants,tocopherol cyclase(TC/VTE1) catalyzes the conversion of 2,3-dimethyl-5-phytyl-1,4-benzoquinone(DMPBQ) to γ-tocopherol.In the present study,OsVTE1,which encodes a rice tocopherol cyclase ortholog,was cloned and characterized.OsVTE1 was induced significantly by abiotic stresses such as high salt,H2O2,drought,cold and by the plant hormones ABA and salicylic acid.The tissue-specific expression pattern and OsVTE1-promoter GUS activity assay showed that OsVTE1 was mainly expressed in the leaf,and also could be detected in the root,stem and panicle.Compared with control plants,transgenic plants with OsVTE1 RNA interference(OsVTE1-RNAi) were more sensitive to salt stress whereas,in contrast,transgenic plants overexpressing OsVTE1(OsVTE1-OX) showed higher tolerance to salt stress.The DAB in vivo staining showed that OsVTE1-OX plants accumulated less H2O2 than did control plants. | OUYANG ShouQiang HE SiJie LIU Peng ZHANG WanKe ZHANG JinSong CHEN ShouYi | 2011 | Science China(Life Sciences)2011，54，2： | 6 |
| 10 | Three-dimensionally ordered, ultrathin graphitic-carbon frameworks with cage-like mesoporosity for highly stable Li-S batteries显示文摘Mesoporous 碳广泛地为锂硫(Li-S ) 作为硫主人被利用了电池。设计碳主人的孔,墙厚度,和 graphitization 度的能力为处理妨碍 Li-S 电池的商品化的问题是必要的,例如快能力腐烂和差的高率的性能。在这个工作,高度订了,有唯一的像笼子的 mesoporosity 的 ultrathin mesoporous graphitic 碳框架(MGF ) ,源于自我装配的 Fe 3 O 4 nanoparticle 超点阵,被表明是为包含硫的一位优秀主人。产生 S@MGFs 展览高度特定的能力(1,446 mAh?? | Huijuan Yu Hanwen Li Shouyi Yuan Yuchi Yang Jiahui Zheng Jianhua Hu Dong Yang YonggangWang Angang Dong | 2017 | Nano Research2017，10，7： | 5 |
| 11 | A novel tobacco gene coding for a product similar to bacterial two-component regulators显示文摘The two-component signaling system has been studied in bacteria. It takes part in signal transduction of adaptive behavior. Recent studies have shown that a similar two-component system is also present in eukaryotes. Examples of this are ETR1 and CKI1 genes which may involve the signal transduction of plant hormone ethylene and cytokinin respectively. The cloning and characterization of a novel gene ( NTHK1 ) fragment from tobacco are presented.Its partial sequence codes for a product which shows similarity to many two-component signaling proteins. Southern blot analysis indicated that there are 2 to 3 copies of NTHK1 gene in tobacco genome (allotetraploid). Homologous genes may also exist in other plants such as Arabidopsis, soybean and spinach. The expression of NTHK1 gene has also been analyzed in tobacco.Further studies on the isolation of full-length cDNA of NTHK1 gene will elucidate more clearly its function in signal perception and transduction. | Jinsong Zhang Can Xie Feng Liu Fenghua Liu Shouyi Chen | 1999 | Chinese Science Bulletin1999，44，11： | 5 |
| 12 | Exploration of presence/absence variation and corresponding polymorphic markers in soybean genome显示文摘This study was designed to reveal the genome‐wide distribution of presence/absence variation(PAV) and to establish a database of polymorphic PAV markers in soybean. The 33 soybean whole‐genome sequences were compared to each other with that of Williams 82 as a reference genome. A total of 33,127 PAVs were detected and 28,912 PAV markers with their primer sequences were designed as the database NJAUSoyPAV_1.0. The PAVs scattered on whole genome while only 518(1.8%) overlapped with simple sequence repeats(SSRs) in BARCSOYSSR_1.0database. In a random sample of 800 PAVs, 713(89.13%) showed polymorphism among the 12 differential genotypes. Using 126 PAVs and 108 SSRs to test a Chinese soybean germplasm collection composed of 828 Glycine soja Sieb. et Zucc. and Glycine max(L.) Merr. accessions, the per locus allele number and its variation appeared less in PAVs than in SSRs. The distinctness among alleles/bands of PCR(polymerase chain reaction) products showed better in PAVs than in SSRs, potential in accurate marker‐assisted allele selection. The association mapping results showed SSR t PAV was more powerful than any single marker systems.The NJAUSoyPAV_1.0 database has enriched the source of PCR markers, and may fit the materials with a range of per locus allele numbers, if jointly used with SSR markers. | Yufeng Wang Jiangjie Lu Shouyi Chen Liping Shu Reid G.Palmer Guangnan Xing Yan Li Shouping Yang Deyue Yu Tuanjie Zhao Junyi Gai | 2014 | Journal of Integrative Plant Biology2014，56，10： | 5 |
| 13 | Ethylene and salicylic acid synergistically accelerate leaf senescence in Arabidopsis显示文摘The phytohormones ethylene and salicylic acid(SA) have long been known to promote senescence, but their interplay during this process remains elusive. Here we report the synergistic effects of ethylene and SA on promoting leaf senescence in Arabidopsis. EIN3, a key transcription factor of ethylene signaling, physically interacted with the core SA signaling regulator NPR1 in senescing leaves. EIN3 and NPR1 synergistically promoted the expression of the senescence-associated genes ORE1 and SAG29.The senescence phenotype was more delayed for the ein3 eil1 npr1 triple mutant than ein3 eil1 or npr1 with ethylene or/and SA treatment. NPR1-promoted leaf senescence may depend on functional EIN3/EIL1. | Chaoqi Wang Shouyi Dai Zhong‐Lin Zhang Wenqing Lao Ruiying Wang Xianqing Meng Xin Zhou | 2021 | Journal of Integrative Plant Biology2021，63，5： | 4 |
| 14 | Cloning,expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco显示文摘Nucleoside diphosphate kinase (NDPK ) 是维持除了 ATP,在生合成使用的所有(d) NTP 的细胞内部的层次的家务酶。这里,我们报导编码 nucleoside diphosphate kinase (NDPK ) 的全身的 cDNA 用酵母被克隆二混血儿的途径。烟草 NDPK 基因被获得并且指定了为 NtNDPK1。NtNDPK1 是在长度的 704 bp 并且编码一通常认为 148 氨基酸的 16.2 kD 蛋白质。系统发生的分析证明 NtNDPK1 对另外的植物 NDPK 基因高度相应并且作为类型 i (NDPK1 ) 识别。RNA 胶化污点分析证明没有在根的 NtNDPK1 表示的重要差别,起源,离开并且发芽。并且 NtNSPK1 的表示被骆驼毛的织物和木钉导致并且由 NaCl 镇压了,然而并非显著地由 Paraquat 影响了,伤害并且低温度(4 ° C ) 处理, NtNDPK1 可以玩的指示响应不能生活的应力的某个角色。在 vitrophosphorylation,试金证明 NtNDPK1 举办了 autophosphorylation 活动。 | Zhou Qiyun Xie Zongming Zhang Zhigang Cao Yangrong Zhang jinsong Chen Shouyi | 2007 | Progress in Natural Science:Materials International2007，17，8： | 4 |
| 15 | Mapping Quantitative Trait Loci Associated with Aluminum Toxin Tolerance in NJRIKY Recombinant Inbred Line Population of Soybean (Glycine max)显示文摘To investigate the genetic mechanism of Al-tolerance in soybean, a recombinant inbred line population (RIL) with 184 F2:7:11 lines derived from the cross of Kefeng No.1 × Nannong 1138-2 (Al-tolerant × Al-sensitive) were tested in pot experiment with sand culture medium in net room in Nanjing. Four traits, i.e. plant height, number of leaves, shoot dry weight and root dry weight at seedling stage, were evaluated and used to calculate the average membership index (FAi) as the indicator of Al-tolerance. The composite interval mapping (CIM) under WinQTL Cartographer v. 2.5 detected five QTLs (i.e. qFAi-1, qFAi- 2, qFAi-3, qFAi-4 and qFAi-5), explaining 5.20%-9.07% of the total phenotypic variation individually. While with the multiple interval mapping (MIM) of the same software, five QTLs (qFAi-1, qFAi-5, qFAi-6, qFAi-7, and qFAi-8) explaining 5.7%-24.60% of the total phenotypic variation individually were mapped. Here qFAi-1 and qFAi-5 were detected by both CIM and MIM with the locations in a same ?anking marker region, GMKF046-GMKF080 on B1 and satt278-sat_95 on L, respectively. While qFAi-2 under CIM and qFAi-6 under MIM both on D1b2 were located in neighboring regions with their confidence intervals overlapped and might be the same locus. Segregation analysis under major gene plus polygene inheritance model showed that Al-tolerance was controlled by two major genes (h2mg = 33.05%) plus polygenes (h2pg = 52.73%). Both QTL mapping and segregation analysis confirmed two QTLs responsible for Al-tolerance with relatively low heritability, and there might be a third QTL, confounded with the polygenes in segregation analysis. | Bo Qi Paul Korir Tuanjie Zhao Deyue Yu Shouyi Chen Junyi Gai | 2008 | Journal of Integrative Plant Biology2008，50，9： | 4 |
| 16 | Stepwise prediction and statistical screening:B-cell epitopes on neuraminidase of human avian H_5N_1 virus显示文摘The B-cell epitopes of virus are associated with the antiviral drug and the vaccine screening. As the nucleotide sequences of neuraminidase (NA) of stain GD-01-06 were sequenced, we predicted the α-helix and β-fold structure and the indexes of the flexible regions of secondary structure of NA with methods of the Hydrophilicity plot by Kyte-Doolittle, the Surface probability plot by Emini and the Antigenic index by Jameson-Wolf, and then screened statistically the parameters to predict B-cell epitopes by the Hierarchical cluster and the Bivariate correlation and the quartiles with SPSS 13.0. The impact of variation of amino acids in NA on its epitopes was analyzed. The predictive results were evaluated by Wu’s Antigenic Index and SWISS-MODEL. We found that the most possible epitopes on NA were located within or nearby its N-terminal Nos. 120―137, 81―84, 408―415, 273―282, 429―432, 356―368, 46―55, 146―155, 341―350 and 198―209, which were the dominant regions of NA epitopes. Peptide 120―137 including the glycoprotein domain (NGT126―128) was first chosen as the B-cell epitopes on NA. NA in H5N1 strain isolated after 2003 lacked in No. 53 amino acid (I), resulting in an increase in the surface flexible region of NA in GD-01-06 and an enlargement to their epitope regions (VEP46―48→ VEPISNTNFL46―55). Conclusively, prediction of the B-cell epitopes on the NA based on multiple parameters is useful for researches on the molecular immunology and drug screening and immuno-prophylaxis. A deletion of No. 53 amino acid (I) in NA in strain GD-01-06 might increase its antigenicity. | HUANG Ping YU ShouYi KE ChangWen | 2008 | Chinese Science Bulletin2008，53，23： | 4 |
| 17 | Isolation and characterization of soybean NBS analogs显示文摘Isolation of plant resistance genes is greatly helpful to crop resistance breeding and the insight of resistance mechanism. The cloned plant resistance genes are classified into four classes according to their putative structural domain, of which the majority possesses nucleotide-binding site (NBS) domain that consists of P-loop, kinase2a and kinase3a. The conservation of this domain affords the potential possibility of cloning the plant resistance genes, which is homology-based cloning technique. In the present study, the degenerate oligonucleotide primers were designed according to the tobacco N and Arabidopsis RPS2, and 358 clones were isolated from the genomic DNA of resistance soybean culti-var Kefengl, resistant to soybean mosaic virus, and 4 open-reading NBS analogs were finally characterized and designated as KNBS1, KNBS2, KNBS3 and KNBS4. Southern hybridization suggested that they were present with multicopy in the soybean genome; KNBS4 was mapped to F linkage group and KNBS2 co-located J | Chaoying He Zhiyong Zhang Shouyi Chen | 2001 | Chinese Science Bulletin2001，46，23： | 3 |
| 18 | Molecular characterization of RAPD and SCAR marker linked to the frog-eye leaf spot resistance gene in soybean显示文摘Two fragments SCS362o and SCS3580 of the co-dominant marker OPS0362o&580 that were linked to the resistance gene of soybean frog-eye leaf spot have been completely sequenced. A significant insertion of 30 bp is the main reason of the polymorphism between the two fragments. The results of Southern hybridization indicate that SCS362o derives from a single- or low-copy sequence and can be used as an RFLP probe. A co-dominant SCAR marker SCSSeao&sao has been developed based on the sequences. The segregation of SCS362o&58o is similar to that of RAPD marker OPS03620&580. Significant polymorphism has been shown between resistant and susceptible genotypes when 62 soybean genotypes were surveyed for the SCAR marker. Therefore, the marker can be used in the resistance breeding of soybean frog-eye leaf spot by marker-assisted selection. | ZOU Jijun YANG Qingkai CHEN Shouyi CHEN Qingshan LIU Yaguang DONG Wei | 2000 | Chinese Science Bulletin2000，45，5： | 3 |
| 19 | Row-based configuration mechanism for a 2-D processing element array in coarse-grained reconfigurable architecture显示文摘Using the coarser operand grain and simplified interconnection patterns, CGRA(coarse grained reconfigurable architectures) has been proven to be energy efficient in several specific domains. As we know, the speed at which the contexts are applied to a PEA(processing element array) directly determines the performance of CGRA. In this paper, the design space in CGRA is further developed from the configuration granularity perspective by one middle-grained configuration granularity—the row-based configuration mechanism(RCM).The most prominent feature of the RCM is that a large DFG(data flow graph) can be mapped onto a small array in once reconfiguration, which is carried out on a row-by-row basis. Compared with an ordinary DFGpartitioning solution, the reconfiguration time and the data transfer time are well reduced. Furthermore, the proposed RCM offers much more efficient storage for the contexts. Compared with the DFG partitioning solution,the performance is boosted from 2.6% to 57.8%, while the area penalty is only 4.79% and the power penalty is only 7.22%. The RCM has been used in one reconfigurable processor called REMUS HPA(reconfigurable multi-media system, high performance version advanced). REMUS HPA has been implemented on a 50.5 mm2 silicon with TSMC 65 nm technology. Simulation shows that 1920×1088@37 fps can be achieved for H.264high-profile decoding when exploiting a 200 MHz working frequency. Compared with the high performance version of XPP(one commercial reconfigurable processor), the performance is 247% boosted. | LIU LeiBo WANG YanSheng YIN ShouYi ZHU Min WANG Xing WEI ShaoJun | 2014 | Science China(Information Sciences)2014，57，10： | 3 |
| 20 | Characterization of Arabidopsis calreticulin mutants in response to calcium and salinity stresses显示文摘作为重要钙绑定蛋白质, calreticulin 在在 endoplasmic 蜂窝胃调整钙动态平衡起一个重要作用(嗯) 植物。这里,我们在 Arabidopsis 识别了 calreticulin 基因的三 loss-of-function 异种响应钙和咸度压力表明 calreticulin 的功能。有三基因,把 calreticulin 编码为 Arabidopsis,并且他们被称为 AtCRT1, 2,和 3 分别地。我们发现两个挑选 crt3 的异种和 crt1 crt2 的两倍异种比野类型的 Arabidopsis 对低钙环境更敏感。而且, crt3 异种显示了更多的敏感在萌芽舞台,而是忍耐腌处理与野类型的植物相比在以后的舞台腌应力。然而,与在钙和盐压力下面的野类型的 Arabidopsis 相比在变异的 crt1 和 crt2 没有明显的生长差别。这些结果建议 calreticulin 在对钙和盐压力的植物回答工作。 | Zhigang Li Yangrong Cao Jinsong Zhang Shouyi Chen | 2008 | Progress in Natural Science:Materials International2008，18，10： | 3 |
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