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| 1 | Increased catabolism and decreased unsaturation of ganglioside in patients with inflammatory bowel disease显示文摘AIM: To investigate whether accelerated catabolism of ganglioside and decreased ganglioside content contribute to the etiology of pro-inflammatory intestinal disease. METHODS: Intestinal mucosa from terminal ileum or colon was obtained from patients with ulcerative colitis or inflammatory Crohn's disease(n = 11) undergoing bowel resection and compared to control samples of normal intestine from patients with benign colon polyps(n = 6) and colorectal cancer(n = 12) in this observational case-control study. Gangliosides and phospholipids of intestinal mucosa were characterized by class and ceramide or fatty acid composition using liquid chromatography triple-quad mass spectrometry. Content and composition of ganglioside classes GM1, GM3, GD3, GD1 a, GT1 and GT3 were compared among subject groups. Content and composition of phospholipid classes phosphatidylcholine(PC) and phosphatidylethanolamine were compared among subject groups. Unsaturation index of individual ganglioside and phospholipid classes was computed and compared among subject groups. Ganglioside catabolism enzymes beta-hexosaminidase A(HEXA) and sialidase-3(NEU3) were measured in intestinal mucosa using western blot and compared among subject groups. RESULTS: Relative GM3 ganglioside content was 2-fold higher(P < 0.05) in intestine from patients with inflammatory bowel disease(IBD) compared to control intestine. The quantity of GM3 and ratio of GM3/GD3 was also higher in IBD intestine than control tissue(P < 0.05). Control intestine exhibited 3-fold higher(P < 0.01) relative GD1 a ganglioside content than IBD intestine. GD3 and GD1 a species of ganglioside containing three unsaturated bonds were present in control intestine, but were not detected in IBD intestine. The relative content of PC containing more than two unsaturated bonds was 30% lower in IBD intestine than control intestine(P < 0.05). The relative content of HEXA in IBD intestine was increased 1.7-fold(P < 0.05) and NEU3 was increased 8.3-fold(P < 0.01) compared to normal intestine. Intestinal mucosa in IBD is characterized by increased GM3 content, decreased GD1 a, and a reduction in polyunsaturated fatty acid constituents in GD3, GD1 a and PC.CONCLUSION: This study suggests a new paradigm by proposing that IBD occurs as a consequence of increased metabolism of specific gangliosides. | John J Miklavcic Glen K Shoemaker Vera C Mazurak M Tom Clandinin Tasha DL Hart Kareena L Schnabl Gordon M Lees Bodil MK Larsen Oliver F Bathe Alan BR Thomson M Tom Clandinin | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 2 | 中美公众的转基因态度差异及公众质疑转基因原因探析显示文摘转基因是世界范围内的热点话题,在中国这一争论又变得更为复杂。本研究通过平行问卷调查的方式,对比了中美公众对转基因的态度差异。调查结果显示:中国公众自认为了解转基因的仅为26%,低于美国的40%。只有17.8%的中国公众认为转基因食品是安全的,低于美国的39%。为了进一步分析中国公众质疑转基因的原因,研究梳理总结出14个转基因负面传言,涵盖了食品安全、科技进步、环境保护、社会公平、政府透明、宗教信仰等多个方面。结果显示:这些传言在公众中均具有一定的知晓度和认可度。其中,65.9%的公众都认同传言“很多大型国际活动(奥运会、世博会等)都禁止提供转基因食品”,45.3%的公众都认同传言“美国孟山都公司毁灭中国大豆产业,转基因是巨大的阴谋,是针对发展中国家的生物武器”。由此可见,转基因争论已经超出了科学范畴,不能单纯依靠科学来回应公众质疑,科学传播的内容也需要进行“供给侧改革”。 | 崔凯 Sharon Shoemaker | 2020 | 华中农业大学学报(社会科学版)2020，，6： | 3 |
| 3 | BMP decision support system for evaluating stormwater management alternatives显示文摘Prince George’s County,Maryland,in the Washington D.C.metropolitan area has developed a best management practice decision support system(BMPDSS)to support analysis and decision making for stormwater management planning and design at both the site scale and the watershed levels.This paper presents a detailed description of the BMPDSS.A case study that demonstrates the application of the system is also included.The case study involves a Green Highway project located in a highly urbanized area within the Anacostia River watershed of the county.Several best management practices(BMP)such as bioretention,filter vegetative swale,porous paving,and landscape infiltration are proposed for reducing highway runoff and improving water quality.The BMPDSS is used to identify and evaluate various alternatives to determine the most costeffective types and combinations of BMPs that minimize the highway runoff pollution. | Mow-Soung CHENG Jenny X.ZHEN Leslie SHOEMAKER | 2009 | Frontiers of Environmental Science & Engineering2009，3，4： | 3 |
| 4 | Outcome prediction of emergency patients by noninvasive hemodynamic monitoring显示文摘 | Shoemaker W C Wo C C Ramicone E | 2001 | Chest2001，120，： | 2 |
| 5 | An investigation of the chemical stability of arsenosugar in simulated gastric juice and acidic environments using IC-ICP-MS and IC-ESIMS/MS显示文摘 | GAMBLE B M GALLAGHER P A SHOEMAKER J A | 2002 | Analyst2002，127，： | 1 |
| 6 | Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides 显示文摘 | Bennett CF Chiang MY Chan H shoemaker JE Mirabelli CK | 1992 | Mol Pharmacol1992，41，6： | 1 |
| 7 | Outcome prediction of emergency patients by noninvasive hemodynamic monitoring 显示文摘 | Shoemaker WC Wo CCJ Chan L | 2001 | Chest2001，11，： | 1 |
| 8 | Comparative pharmacokinetics and bioavailability of oxolinic acid in channel catfish (Ictalurus punctatus) and rainbow trout (Oncorhynchus mykiss)显示文摘 | Kleinow K M Jarboe H H Shoemaker K E | 1994 | Can J Fish Aquac Sci1994，51，： | 1 |
| 9 | Experimental annotation of the human genome using microarray technology显示文摘 | Shoemaker D D Schadt E E Armoar L | 2001 | Nature2001，409，22： | 1 |
| 10 | A new integrated genetic linkage map of the soybean显示文摘 | Song Q J Marek L F Shoemaker R C | 2004 | Theoretial and Applied Genetics2004，109，： | 1 |
| 11 | Randomized Comparison of a High Clopidogrel Maintenance Dose in Patients With Diabetes Mellitus and Coronary Artery Disease Results of the Optimizing Antiplatelet Therapy in Diabetes Mellitus (OPTIMUS) Study显示文摘 | Angiolillo DJ Shoemaker SB Desai B | 2007 | Circulation2007，115，6： | 1 |
| 12 | Loyalty:A strategic commitment显示文摘 | Bowen J T Shoemaker S | 1998 | Cornell Ho- tel and Restaurant Administration Quarterly1998，39，1： | 1 |
| 13 | Bench to bedside: electrophysiologic and clinical principles of noninvasive hemodynamic monitoring using impedance cardiography显示文摘 | Summers RL Shoemaker WC Peacock WF | 2003 | Acad Emerg Med2003，10，6： | 1 |
| 14 | Production and processing of milk from transgenic goats expressing human lysozyme in the mammary gland 显示文摘 | MAGA E A SHOEMAKER C F ROWE J D | 2006 | Journal of Dairy Science2006，89，2： | 1 |
| 15 | Characterization of endo-1,4-beta-D-glucanases purified from Trichoderma viride显示文摘 | SHOEMAKER S P BROWN R D | 1978 | Biochim Biophys Aeta1978，523，： | 1 |
| 16 | Sequence of physiologic patterns in physiology in surgical septic shock显示文摘 | Shoemaker WC Appel PL Kram HB | 1993 | Crit Care Med1993，21，12： | 1 |
| 17 | Design and testing of PCR primers for the construction of scFv libraries representing the immunoglobulin repertoire of rats显示文摘 | Sepulveda J Shoemaker C B | 2008 | J Immunol Methods2008，332，12： | 1 |
| 18 | A new integrated ge-netic linkage map of the soybean显示文摘 | Song Q J Marek L F Shoemaker R C | 2004 | Theoretical and Applied Ge-netics2004，109，： | 1 |
| 19 | Meta-analysis of hemodynamic optimisation in high-risk patients显示文摘 | Kern JW Shoemaker WC | 2002 | Crit Care Med2002，30，： | 1 |
| 20 | Rapid deteetion of columnaris disease in channel catfish (Ictalurus punctatus) with a new species-specific 16S rRNA gene-based PCR primer for Flavobacterium columnare显示文摘 | BaderJA Shoemaker CA Klesius PH | 2003 | J Mierobiol Methods2003，52，2： | 1 |
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