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| 1 | Ultrafast photonic PCR显示文摘Nucleic acid amplification and quantification via polymerase chain reaction(PCR)is one of the most sensitive and powerful tools for clinical laboratories,precision medicine,personalized medicine,agricultural science,forensic science and environmental science.Ultrafast multiplex PCR,characterized by low power consumption,compact size and simple operation,is ideal for timely diagnosis at the point-of-care(POC).Although several fast/ultrafast PCR methods have been proposed,the use of a simple and robust PCR thermal cycler remains challenging for POC testing.Here,we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon–electron–phonon coupling.We demonstrate an efficient photonic heat converter using a thin gold(Au)film due to its plasmon-assisted high optical absorption(approximately 65%at 450 nm,the peak wavelength of heat source light-emitting diodes(LEDs)).The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150℃ within 3 min.Using this method,ultrafast thermal cycling(30 cycles;heating and cooling rate of 12.7960.93℃ s^(-1) and 6.660.29℃ s^(-1),respectively)from 55℃(temperature of annealing)to 95℃(temperature of denaturation)is accomplished within 5 min.Using photonic PCR thermal cycles,we demonstrate here successful nucleic acid(λ-DNA)amplification.Our simple,robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures,including on-chip thermal lysis and heating for isothermal amplifications. | Jun Ho Son Byungrae Cho SoonGweon Hong Sang Hun Lee Ori Hoxha Amanda J Haack Luke P Lee | 2015 | Light(Science & Applications)2015，4，1： | 2 |
| 2 | Protein phosphatase PP4 is overexpressed in human breast and lung tumors显示文摘 | Bo Wang Ailian Zhao Lingling Sun Xueyan Zhong Jianing Zhong Haibo Wang Minhua Cai Jing Li Yi Xu Ji Liao Jianli Sang Dipanj an Chowdhury Gerd P Pfeifer Yun Yen Xingzhi Xu | 2008 | Cell Research2008，18，9： | 2 |
| 3 | The Regional Innovation System in Sweden: a Study of Regional Clusters for the Development of High Technology 显示文摘 | SANG C P LEE S K | 2004 | AI & SOCIETY2004，18，3： | 1 |
| 4 | Antimicrobial fabrication of cotton fabric and leather rising 显示文摘 | Velmurugan P Min Cho Sang - Myeong Lee | 2014 | Car- bohydrate Polymers2014，106，15： | 1 |
| 5 | 显示文摘 | Dong S S Sang T P Hoon S C | 1999 | Thin Solid Films1999，354，： | 1 |
| 6 | Effect of vacuum thermal cyclic exposures on unidirectional carbon fiber/epoxy composites for low earth orbit space applications 显示文摘 | Sang Y P Heung S C Won J C | 2012 | Composites : Part B2012，43，2： | 1 |
| 7 | Structure and Functional Properties of Sorghum Starches Differing in Amylose Content 显示文摘 | Sang Y J Bean S Seib P A | 2008 | Agrie Food Chem2008，56，16： | 1 |
| 8 | Fabrication of Mg-doped ZnO Thin Films by Laser Ablation of Zn: Mg Target显示文摘 | Tae H K Jin J P Sang H N | 2009 | Applied Surface Science2009，255，10： | 1 |
| 9 | Analysis of static axial load capacity of single piles and large diameter shafts using nonlinear load transfer curves显示文摘 | HYEONG J K MISSION J L C SANG P | 2007 | KSCE Journal of Civil Engineering2007，11，6： | 1 |
| 10 | Finite Element Analysis and Design in Stainless Steel Sheet Forming and Its Experimental Comparison 显示文摘 | Li P L Sang M H Beom S K | 2001 | Journal of Materials Processing Technology2001，110，1： | 1 |
| 11 | Effect of milling ratio on sensory properties of cooked rice and on physicochemical properties of milled and cooked rice 显示文摘 | Jung K P Sang S K Kwang O K | 2001 | Cereal Chemistry2001，78，2： | 1 |
| 12 | Speciation of chromium in water samples with cloud point extraction separation and preconcentration and determination by graphite furnace atomic absorption spectrometry显示文摘 | LIANG P SANG H | 2008 | Journal of Hazardous Materials2008，154，13： | 1 |
| 13 | Determination of the curvature of the magnetic fluid under the external forces显示文摘 | GWAN S P SANG H P | 2002 | IEEE Transactions on Magnetics2002，38，2： | 1 |
| 14 | Fo-cused ion beam fabrication of submicron gold struc-tures显示文摘 | Blauner P G Butt Yousaf Ro Jae Sang | 1989 | Vac Sci Technol B1989，7，4： | 1 |
| 15 | Speciation of chromium in water samples with cloud point extraction separation and preconcentration and determination by graphite furnace atomic absorption spectrometry显示文摘 | Liang P Sang H B | 2008 | Journal of Hazardous Materials2008，154，： | 1 |
| 16 | The relation between periods' identification and noises in hydrologic series data 显示文摘 | Sang Y F Wang D Wu J C Zhu Q P Wang L | 2009 | Journal of Hydrology2009，368，14： | 1 |
| 17 | Organocatalytic Application of Direct Organo-Functionalized Mesoporous Catalysts prepared by Microwave 显示文摘 | SANG P EKO A P | 2009 | Top Cata12009，52，： | 1 |
| 18 | Biochemical and physiological mechanism mediated by allelochemicals显示文摘 | Tiffany L W Sang W P Jorge M V | 2004 | Current Opinion in Plant Biology2004，7，： | 1 |
| 19 | Pseudomonas Aeruginosa Exotoxin A Reduces Chemoresistance of Oral Squamous Carcinoma Cell via Inhibition of Heat Shock Proteins 70 (HSP70)显示文摘 | SANG R P KYOUNG D L UK K K | 2010 | Yonsei Med J2010，51，5： | 1 |
| 20 | Asymptotic homogenization of viscoelastic composites with periodic microstructrues显示文摘 | YEONG M Y SANG H P SUNG K Y | 1998 | Int J Solids Structures1998，35，17： | 1 |
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