|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Hierarchical Nanogold Labels to Improve the Sensitivity of Lateral Flow Immunoassay显示文摘Lateral flow immunoassay(LFIA) is a widely used express method and offers advantages such as a short analysis time, simplicity of testing and result evaluation.However, an LFIA based on gold nanospheres lacks the desired sensitivity, thereby limiting its wide applications.In this study, spherical nanogold labels along with new types of nanogold labels such as gold nanopopcorns and nanostars were prepared, characterized, and applied for LFIA of model protein antigen procalcitonin. It was found that the label with a structure close to spherical provided more uniform distribution of specific antibodies on its surface, indicative of its suitability for this type of analysis.LFIA using gold nanopopcorns as a label allowed procalcitonin detection over a linear range of 0.5–10 ng mL^(-1) with the limit of detection of 0.1 ng mL^(-1), which was fivefold higher than the sensitivity of the assay with gold nanospheres. Another approach to improve the sensitivity of the assay included the silver enhancement method,which was used to compare the amplification of LFIA for procalcitonin detection. The sensitivity of procalcitonin determination by this method was 10 times better the sensitivity of the conventional LFIA with gold nanosphere as a label. The proposed approach of LFIA based on gold nanopopcorns improved the detection sensitivity without additional steps and prevented the increased consumption of specific reagents(antibodies). | Kseniya Serebrennikova Jeanne Samsonova Alexander Osipov | 2018 | Nano-Micro Letters2018，10，2： | 5 |
| 2 | Characteristics of the active-layer under the China-Russia Crude Oil pipeline显示文摘Active layer is a key component for permafrost environment studies as many subsurface biological,biogeochemical,ecological,and pedogenic activities prevail in this layer.This study focuses on active layer temperature monitoring in an area with sporadic permafrost at two adjacent sites along China-Russia Crude Oil Pipeline(CRCOP),NorthEast China.Site T1 is located in disturbed ground on the right-of-way(on-ROW)2 m away from the center of the oil pipeline.T2 is located in a natural and undisturbed site,around 16.6 m off-ROW.Our objective was to study seasonal variability of the active layer depth and thermal regime from October 2017 to September 2018.The monitoring sites consist of soil temperature probes arranged in a vertical array at different depths at both sites.The following parameters were computed:number of isothermal days(ID),freezing days(FD),thawing days(TD),freezing degree days(FDD),thawing degree days(TDD),number of freeze-thaw days(FTD).The mean air temperature in the monitoring period reached-3.2℃.The temperature profile indicates that the maximum active layer thickness observed during the study period was 10 m at T1 and 2 m at T2.The majority of the soil temperatures were above or close to 0℃,resulting in great values of TDD,especially in the first 4 m depth.TDD for T1 were predominant and ranged between 600-1160℃·days(0-4 m depth)reflecting the influence of oil temperature from the pipeline.In T2 borehole FDD were predominant for all the soil layer depths resulting in less permafrost degradation.This comparison emphasizes the significant influence of vegetation removal and the dispersed heat from the pipeline on the active layer thickness. | SERBAN Mihaela LI Guo-yu SERBAN Raul-David WANG Fei FEDOROV Alexander VERA Samsonova CAO Ya-peng CHEN Peng-chao WANG Wei | 2021 | Journal of Mountain Science2021，18，2： | 4 |
| 3 | Enzyme-linked im- munosorbent assay of ampicillin in milk显示文摘 | Samsonova Z Shchelokova O lvanova N | 2005 | Applied Biochemis- try Andmierobiology2005，41，6： | 1 |
| 4 | Moiré interference technique for studying vortex flame structures显示文摘 | SAMSONOV V P SAMSONOVA I V A | 2005 | Technical Physics Letters2005，31，2： | 1 |
| 5 | Enzyme immunoassay for semicarbazide--The nitrofuran metabolite and food contaminant 显示文摘 | Cooper K M Samsonova J V | 2007 | Analytica Chimica Acta2007，592，： | 1 |
| 6 | Determination of ivermectin in bovine liver by optical immunobiosensor显示文摘 | SAMSONOVA J V BAXTER G A CROOKS S R | 2002 | Biosensors and Bioelectronics2002，17，67： | 1 |
| 7 | Gestational hypothyroxinemia and cognitive function in offspring显示文摘 | Kasatkina EP Samsonova LN Ivakhnenko VN | 2006 | Neurosci Behav Physiol2006，36，6： | 1 |
| 8 | Polarization fluoroimmunoassay of propazine in reversed micelles of aerosol OT in n-octane 显示文摘 | Matvccva E G Samsonova Z V Eremin S A | 1996 | Russian Journal of Bioorganic Chemistry1996，22，12： | 1 |
| 9 | Enzyme-linked immunosorbent assay of ampicillin in milk 显示文摘 | Samsonova Z Shchelokova O Ivanova N | 2005 | Applied Biochemistry Andmicrobiology2005，41，6： | 1 |
| 10 | Enzyme immunoassay for semicar- bazide-The nitrofuran metabolite and food con- taminant显示文摘 | COOPER K M SAMSONOVA J V PLUMPT- ON L | 2007 | Analytica Chimica Acta2007，592，： | 1 |
| 11 | Biosensor immunoassay of ivermectin in bovine milk显示文摘 | Samsonova JV Baxter GA Crooks SRH | 2002 | J AOAC Int2002，85，4： | 1 |
| 12 | Enzyme-Linked Immunosorbent Assay of Ampicillin in Milk 显示文摘 | Samsonova Zh V Shchelokova O S Ivanova N L | 2005 | Applied Biochemistry and microbiology2005，41，6： | 1 |
| 13 | Enzyme - linked immunosorbent assay of ampicillin in milk显示文摘 | Samsonova Zhv Shchelokova OS Ivanova NL | 2005 | Prikl Biokhim Mikrobiol2005，41，6： | 1 |
| 14 | Aspartate-and alanine aminotransfer ase in early development of the keta显示文摘 | Samsonova M V Minkova N O Lapteva T I | 2003 | Russian Journal of Develo-pmental Biology2003，34，1： | 1 |
| 15 | Competitive ELISA of chloramphenicoh influence of imnunoreagent structure and application of the method for the inspection of food of animal origin 显示文摘 | KOLOSOVA A Y SAMSONOVA J V EGOROV A M | 2000 | Food Agri Immunol2000，12，2： | 1 |
| 16 | Aspartate-artd alanine-aminotransfer-ase in early development of keta 显示文摘 | Samsonova M V Minkova N O Lapteva T I | 2003 | Ontogenez2003，34，1： | 1 |
| 17 | Metabolic engineering of Escherichia coli to produce (2S, 3R, 4S)-4- hydroxyisoleucine 显示文摘 | Smirnov S V Kodera T Samsonova N | 2010 | App Microbiol Biotech2010，88，3： | 1 |
| 18 | A novel L-isoleucine hydroxylating enzyme, L-isoleucine dioxygenase from Bacillus thuringieusis, produces ( 2S, 3R, 4S ) -4-hydroxyisoleucine 显示文摘 | Kodera T Smirnov S V Samsonova N | 2009 | Biochem Biophys Res Commun2009，390，3： | 1 |
| 19 | Enzyme-linked immunosorbent assay of ampicillin in milk显示文摘 | SAMSONOVA Z SHCHELOKOVA O IVANOVA N | 2005 | Appl Biochem Andmicrobiol2005，41，6： | 1 |
| 20 | Immunohistochemical profile of endometrioid adenocarcinoma of the uterus:ER,PR,HER-2,Ki-67 and their prognostic value显示文摘 | Pozharisskii K M Samsonova E A Ten V P | 2005 | Arkh Patol2005，67，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员