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    题名 作者 年代 出处 被引量
1左氧氟沙星三联疗法治疗顽固性Hp感染优于铋剂四联疗法显示文摘Saad RJ Schoenfeld P Kim HM 2006中国处方药2006,5,5:40
2Methods for in vitro evaluating antimicrobial activity: A review显示文摘In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cytofluorometric and bioluminescent methods are not widely used because they require specified equipment and further evaluation for reproducibility and standardization, even if they can provide rapid results of the antimicrobial agent's effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported.mounyr balouiri moulay sadiki saad koraichi ibnsouda 2016Journal of Pharmaceutical Analysis2016,6,2:38
3Sperm DNA damage in men from infertile couples显示文摘瞄准:为了调查精子 DNA 的高水平的流行,在人之中损坏从不肥沃结合正常和反常标准精液参数。方法:从不肥沃的夫妇的 350 个人的一个总数被估计。标准精液分析和精子染色质结构试金(SCSA ) 被执行。结果:97 个人(28% 整个学习组) 有一个 DNA 破碎索引(DFI )20% ,和 43 人(12%) 有的 > DFI > 30% 。在有反常精液参数的人的组(n= 224 ) , 35% 有 DFI > 20% ,和 16% 有 DFI > 30% 而分别地,这些数字在有正常精液参数的人的组是 15% 和 5%(n= 126 ) 。有低精子活动性和反常形态学的人为有 DFI > 有显著地更高的机会比率(ORs )20%(4.0 为活动性并且 1.9 为形态学) 并且 DFI > 30%(6.2 为活动性并且 2.8 为形态学) 与有正常精子活动性和形态学的人相比。结论:在从不肥沃的夫妇的几乎三分之一个个 unselected 人, DFI 超过了水平 20% 在哪个上面根据以前的研究,在里面 vivo 富饶被减少。有不那样正常的精液参数的人的一个重要比例也让高精子 DNA 损坏层次。因此, SCSA 测试能增加在精液分析没从标准显示出任何偏差的情况中解释不孕的原因。我们也建议运用 SCSA 测试选择适当帮助繁殖技术(艺术) 。Juris Erenpreiss Saad Elzanaty Aleksander Giwercman 2008Asian Journal of Andrology2008,10,5:33
4Radioembolization for the treatment of unresectable hepatocellular carcinoma:A clinical review显示文摘Hepatocellular carcinoma(HCC)is the sixth most common cancer in the world.The majority of patients with HCC present with unresectable disease.These patients have historically had limited treatment options secondary to HCC demonstrating chemoresistance to the currently available systemic therapies.Additionally, normal liver parenchyma has shown intolerance to tumoricidal radiation doses,limiting the use of external beam radiation.Because of these limitations,novel percutaneous liver-directed therapies have emerged. The targeted infusion of radioactive microspheres (radioembolization)represents one such therapy. Radioembolization is a minimally invasive transcatheter therapy through which radioactive microspheres are infused into the hepatic arteries that supply tumor. Once infused,these microspheres traverse the hepatic vascular plexus and selectively implant within the tumor arterioles.Embedded within the arterioles, the 90Y impregnated microspheres emit high energy and low penetrating radiation doses selectively to the tumor.Radioembolization has recently shown promise for the treatment of patients with unresectable HCC. The objective of this review article is to highlight twocurrently available radioembolic devices(90Y,188Rh)and provide the reader with a recent review of the literature.Saad M Ibrahim Robert J Lewandowski Kent T Sato Vanessa L Gates Laura Kulik Mary F Mulcahy Robert K Ryu Reed A Omary Riad Salem 2008World Journal of Gastroenterology2008,14,11:14
5Numerical and experimental comparison of the vaned diffuser interaction inside the impeller velocity field of a centrifugal pump显示文摘The paper refers to the analysis of interactions between the impeller and the vaned diffuser based on the air model of a radial flow pump.The study deals with a numerical simulation of the flow for a full 360°entire impeller and diffuser.The task is carried out closely under the design operating conditions and for one particular position of the impeller blade with respect to the diffuser frame.Among all the results,the focus is mainly on the flow pattern at the exit part inside the impeller facing the diffuser vanes.The results are compared to the available PIV measurements.ATIF Abdelmadjid BENMANSOUR Saad BOIS Gerard DUPONT Patrick 2011Science China(Technological Sciences)2011,54,2:13
6单髁与全膝关节置换术治疗膝单间室骨性关节炎对患者围手术期凝血指标及失血量的影响显示文摘目的:研究单髁膝关节置换术(UKA)与全膝关节置换术(TKA)治疗膝单间室骨性关节炎对患者围手术期凝血指标及失血量的影响。方法:回顾性分析因膝关节单间室骨性关节炎行单侧人工膝关节置换术的77例患者的临床资料。依据手术方式不同将患者分为UKA组(28例)和TKA组(49例)。检测并记录两组患者围术期凝血相关指标及围手术期失血量。记录围手术期下肢深静脉血栓(DVT)及肺栓塞发生情况。结果:两组患者术前D-二聚体、纤维蛋白原(FBG)水平比较差异无统计学意义(均P>0.05),但UKA组术后1 d、手术前后差值及比值低于TKA组(均P<0.05)。两组患者手术前后PT、TT、APTT、INR水平比较,差异无统计学意义(均P>0.05)。两组患者术前红细胞压积(Hct)及血红蛋白(Hb)水平比较差异无统计学意义(均P>0.05),但TKA组术后1、3 d低于UKA组(均P<0.05)。UKA组总失血量、隐性失血量及显性失血量少于TKA组(均P<0.05)。UKA组DVT发生率低于TKA组(P<0.05)。结论:UKA治疗膝关节单间室骨性关节炎,可减少围手术期对患者血液系统的影响,降低失血量及DVT发生率。杨天翔 张晋宁 张博文 程萌旗 陈德胜 2022陕西医学杂志2022,51,6:13
7睾丸活检:临床操作及其诠释显示文摘睾丸活检许多年来被认为是不明病因男性不育和无精症诊断的基础。近来,男性不育指南都限定诊断性睾丸活检只能用于梗阻性无精子症(睾丸体积正常,生殖激素正常)的鉴别诊断。现今,睾丸活检取精技术与胞浆内单精子显微注射(ICSI)联用于非梗阻性无精子症患者的取精过程中。此外,睾丸活检还可以应用于有睾丸恶性肿瘤风险的患者的取精治疗中。研究一些不育男性后发现,不育患者患原位睾丸癌的风险越来越高,尤其是曾有隐睾症、睾丸恶性肿瘤病史,或患睾丸萎缩的患者。睾丸超声异常,比如睾丸微石症,实质内呈弥散分布,软细胞组织不均、睾丸组织损伤等,进一步增加了这些患者原位癌的发病率。准确的组织学分类,正确的组织处理和固定,样品准备和评估是必须的。同时,推荐使用标准的睾丸活检方法。另外,通过免疫组织化学法检测睾丸组织的肿瘤恶化程度是不可缺少的技术支持。本综述主要描述了睾丸活检用于男性不育临床诊断和治疗措施的最新进展。Gert R Dohle Saad Elzanaty Niels J van Casteren 2012Asian Journal of Andrology2012,14,1:13
8CDX2 as a marker for intestinal differentiation: Its utility and limitations显示文摘CDX2 is a nuclear homeobox transcription factor that belongs to the caudalrelated family of CDX homeobox genes. The gene encoding CDX2 is a nonclustered hexapeptide located on chromosome 13q12-13. Homeobox genes play an essential role in the control of normal embryonic development. CDX2 is crucial for axial patterning of the alimentary tract during embryonic development and is involved in the processes of intestinal cell proliferation, differentiation, adhesion, and apoptosis. It is considered specif ic for enterocytes and has been used for the diagnosis of primary and metastatic colorectal adenocarcinoma. CDX2 expression has been reported to be organ specif ic and is normally expressed throughout embryonic and postnatal life within the nuclei of epithelial cells of the alimentary tract from the proximal duodenum to the distal rectum. In this review, the authors elaborate on the diagnostic utility of CDX2 in gastrointestinal tumors and other neoplasms with intestinal differentiation. Limitations with its use as the sole predictor of a gastrointestinal origin of metastatic carcinomas are also discussed.Reda S Saad Zeina Ghorab Mahmoud A Khalifa 2011World Journal of Gastrointestinal Surgery2011,3,11:11
9A combination therapy of ethanol injection and radiofrequency ablation under general anesthesia for the treatment of hepatocellular carcinoma显示文摘AIM: To summarize the effects of laparoscopic ethanol injection and radiofrequency ablation (L-EI-RFA), thora- coscopic (T-EI-RFA) and open-surgery assisted EI-RFA (O-EI-RFA) under general anesthesia for the treatment of hepatocellular carcinoma (HCC). METHODS: Time-lag performance of RFA after ethanol injection (Time-lag PEI-RFA) was performed in all cases. The volume of coagulated necrosis and the applied en- ergy for total and per unit volume coagulated necrosis were examined in the groups treated under general (group G) or local anesthesia (group L). RESULTS: The results showed that the total applied energy and the applied energy per unit volume of whole and marginal, coagulated necrosis were significantly larger in group G than those in the group L, resulting in a larger volume of coagulated necrosis in the group G. The rate of local tumor recurrence within one year was extremely low in group G. CONCLUSION: These results suggest that EI-RFA, un- der general anesthesia, may be effective for the treat- ment of HCC because a larger quantity of ethanol and energy could be applied during treatment under pain- free condition for the patients.Kazutaka Kurokohchi Seishiro Watanabe Hirohito Yoneyama Akihiro Deguchi Tsutomu Masaki Takashi Himoto Hisaaki Miyoshi Hamdy Saad Mohammad Akira Kitanaka Tomohiko Taminato Shigeki Kuriyama 2008World Journal of Gastroenterology2008,14,13:10
10Photovoltaic yield prediction using an irradiance forecast model based on multiple neural networks显示文摘In order to develop predictive control algorithms for efficient energy management and monitoring for residential grid connected photovoltaic systems, accurate and reliable photovoltaic(PV) power forecasts are required.A PV yield prediction system is presented based on an irradiance forecast model and a PV model. The PV power forecast is obtained from the irradiance forecast using the PV model. The proposed irradiance forecast model is based on multiple feed-forward neural networks. The global horizontal irradiance forecast has a mean absolute percentage error of 3.4% on a sunny day and 23% on a cloudy day for Stuttgart. PV power forecasts based on the neural network irradiance forecast have performed much better than the PV power persistence forecast model.Saad Parvaiz DURRANI Stefan BALLUFF Lukas WURZER Stefan KRAUTER 2018Journal of Modern Power Systems and Clean Energy2018,6,2:10
11Prediction of air flow, methane, and coal dust dispersion in a room and pillar mining face显示文摘In underground coal mines, uncontrolled accumulation of methane and fine coal dust often leads to serious incidents such as explosion. Therefore, methane and dust dispersion in underground mines is closely monitored and strictly regulated. Accordingly, significant efforts have been devoted to study methane and dust dispersion in underground mines. In this study, methane emission and dust concentration are numerically investigated using a computational fluid dynamics(CFD) approach. Various possible scenarios of underground mine configurations are evaluated. The results indicate that the presence of continuous miner adversely affects the air flow and leads to increased methane and dust concentrations.Nevertheless, it is found that such negative effect can be minimized or even neutralized by operating the scrubber fan in suction mode. In addition, it was found that the combination of scrubber fan in suction mode and brattice results in the best performance in terms of methane and dust removal from the mining face.Lu Yueze Akhtar Saad Sasmito Agus P. Kurnia Jundika C. 2017International Journal of Mining Science and Technology2017,27,4:10
12Circulating hTERT mRNA as a tumor marker in cholangiocarcinoma patients显示文摘瞄准:调查人的 telomerase 颠倒 transcriptase (hTERT ) 在 cholangiocarcinoma 病人的浆液的 mRNA。方法:三十三个 cholangiocarcinoma 病人,四十一个良性的胆道疾病病人和十个健康志愿者的浆液为由即时反向的 transcriptase 聚合酶链反应(RT-PCR ) 的 hTERT mRNA 的表示镇定、分析。我们然后检验了在浆液 hTERT mRNA 的价值和 cholangiocarcinoma 的病理学的阶段之间的关联。结果:hTERT mRNA 在 33 中的 28 个被检测(84.85%) 从 cholangiocarcinoma 浆液获得了病人,(21.9%) 41 中的 9 个从良性的胆道疾病病人浆液获得了。hTERT mRNA 没在从健康志愿者获得的任何浆液被检测。在另一方面普通肿瘤标记, CA19-9 在 33 中的 20 个被检测(60.6%) 从 cholangiocarcinoma 浆液获得了病人,(19.5%) 41 中的 8 个从良性的胆道疾病病人浆液获得了。然而,没有关联浆液 hTERT mRNA 和肿瘤阶段在礼品之间被发现。结论:这些结果显示传播 hTERT mRNA 的察觉在几乎所有 cholangiocarcinoma 病人被识别。它提供一个新奇肿瘤标记,它能为 cholangiocarcinoma 的诊断被用作互补研究。Kawin Leelawat Surang Leelawat Thawee Ratanachu-Ek Somboon Trubwongchareon Jerasak Wannaprasert Saad Tripongkaruna Suchart Chantawibul Panadda Tepaksorn 2006World Journal of Gastroenterology2006,12,26:10
13Pre-and postoperative systemic hemodynamic evaluation in patients subjected to esophagogastric devascularization plus splenectomy and distal splenorenal shunt:A comparative study in schistomomal portal hypertension显示文摘AIM: To investigate the systemic hemodynamic effects of two surgical procedures largely employed for treatment of schistosomal portal hypertension. METHODS: Thirty-six patients undergoing elective surgical treatment of portal hypertension due to hepatosplenic mansonic schistosomiasis were prospectively evaluated. All patients were subjected to preoperative pulmonary artery catheterization; 17 were submitted to esophagogastric devascularization and splenectomy (EGDS) and 19 to distal splenorenal shunt (DSRS). The systemic hemodynamic assessment was repeated 4 d after the surgical procedure. RESULTS: Preoperative evaluation revealed (mean ± SD) an increased cardiac index (4.78 ± 1.13 L/min per m2),associated with a reduction in systemic vascular resistance index (1457 ± 380.7 dynes.s/cm5.m2). The mean pulmonary artery pressure (18 ± 5.1 mmHg) as well as the right atrial pressure (7.9 ± 2.5 mmHg) were increased,while the pulmonary vascular resistance index (133 ± 62 dynes.s/cm5.m2) was decreased. Four days after EGDS,a significant reduction in cardiac index (3.80 ± 0.4 L/min per m2,P < 0.001) and increase in systemic vascular resistance index (1901.4 ± 330.2 dynes.s/cm5. m2,P < 0.001) toward normal levels were observed. There was also a significant reduction in pulmonary artery pressure (12.65 ± 4.7 mmHg,P < 0.001) and no significant changes in the pulmonary vascular resistance index (141.6 ± 102.9 dynes.s/cm5.m2). Four days after DSRS,a non-significant increase in cardiac index (5.2 ± 0.76 L/min per m2) and systemic vascular resistance index (1389 ± 311 dynes.s/cm5.m2) was observed. There was also a non-significant increase in pulmonary artery pressure (19.84 ± 5.2 mmHg),right cardiac work index (1.38 ± 0.4 kg.m/m2) and right ventricular systolic work index (16.3 ± 6.3 g.m/m2),without significant changes in the pulmonary vascular resistance index (139.7 ± 67.8 dynes.s/cm5.m2). CONCLUSION: The hyperdynamic circulatory state observed in mansonic schistosomiasis was corrected by EGDS,but was maintained in patients who underwent DSRS. Similarly,the elevated mean pulmonary artery pressure was corrected after EGDS and maintained after DSRS. EGDS seems to be the most physiologic surgery for patients with schistosomal portal hypertension.Roberto de Cleva Paulo Herman Luis Augusto Carneiro D’albuquerque Vincenzo Pugliese Orlando Luis Santarem William Abro Saad 2007World Journal of Gastroenterology2007,13,41:9
14Characteristics of common solid liver lesions and recommendations for diagnostic workup显示文摘Due to the widespread clinical use of imaging modalities such as ultrasonography,computed tomography and magnetic resonance imaging (MRI),previously unsuspected liver masses are increasingly being found in asymptomatic patients.This review discusses the various characteristics of the most common solid liver lesions and recommends a practical approach for diagnostic workup.Likely diagnoses include hepatocellular carcinoma (the most likely;a solid liver lesion in a cirrhotic liver) and hemangioma (generally presenting as a mass in a non-cirrhotic liver).Focal nodular hyperplasia and hepatic adenoma should be ruled out in young women.In 70% of cases,MRI with gadolinium differentiates between these lesions.Fine needle core biopsy or aspiration,or both,might be required in doubtful cases.If uncertainty persists as to the nature of the lesion,surgical resection is recommended.If the patient is known to have a primary malignancy and the lesion was found at tumor staging or follow up,histology is required only when the nature of the liver lesion is doubtful.Nimer Assy Gattas Nasser Agness Djibre Zaza Beniashvili Saad Elias Jamal Zidan 2009World Journal of Gastroenterology2009,15,26:9
15锌镁比对Al-Zn-Mg牺牲阳极用于碳钢阴极保护的影响显示文摘研究不同锌镁质量比的Al-Zn-Mg合金作为牺牲阳极在3.5wt.%Na Cl溶液中用于碳钢阴极保护的性能。用纯Al、Zn和Mg金属通过铸造法制备Al-Zn-Mg阳极。采用光学显微镜、扫描电镜-能谱、X射线衍射和电化学等技术对样品进行表征和测试。结果表明,随着锌镁质量比的降低,α(Al)的晶粒尺寸和析出物的粒径减小,析出物的体积分数增大。当锌镁质量比大于4.0时,Al-Zn-Mg阳极具有最低的腐蚀速率;当锌镁质量比小于0.62时,Al-Zn-Mg阳极具有最高的腐蚀速率且对碳钢(AISI 1018)具有最好的阴极保护效果。与其他阳极相比,锌镁质量比为0.62的Al-Zn-Mg阳极具有多孔腐蚀产物。Mosaad SADAWY Saad SAAD Randa ABDEL-KARIM 2020Transactions of Nonferrous Metals Society of China2020,30,8:8
16comparison between tocotrienol and omeprazole on gastric growth factors in stress-exposed rats显示文摘AIM To investigate and compare the effects of tocotrienol and omeprazole on gastric growth factors in rats exposed to water-immersion restraint stress(WIRS).METHODS Twenty-eight male Wistar rats were randomly assigned to four groups of seven rats. The two control groups were administered vitamin-free palm oil(vehicle) and the two treatment groups were given omeprazole(20 mg/kg) or tocotrienol(60 mg/kg) by oral gavage. After 28 d of treatment, rats from one control group and both treated groups were subjected to WIRS one time for 3.5 h. Gastric lesions were measured and gastric tissues were obtained to measure vascular endothelial growth factor(VEGF), epidermal growth factor(EGF), basic fibroblast growth factor(b FGF), and transforming growth factor-alpha(TGF-α) m RNA expression. RESULTS Rats exposed to WIRS for 3.5 h demonstrated the presence of considerable ulcers in the form of gastric erosion. The lesion index in the stressed control(S) group was increased(P < 0.001) compared to the tocotrienol treated and omeprazole treated groups. Stress led to a decrease in gastric VEGF(P < 0.001), b FGF(P < 0.001) and TGF-α(P < 0.001) m RNA levels and caused an increase in EGF m RNA(P < 0.001) that was statistically significant compared to the nonstressed control group. Although both treatment agents exerted similar ulcer reducing ability, only treatment with tocotrienol led to increased expression of VEGF(P = 0.008), b FGF(P = 0.001) and TGF-α(P = 0.002) m RNA.CONCLUSION Tocotrienol provides gastroprotective effects in WIRSinduced ulcers. Compared to omeprazole, tocotrienol exerts a similar protective effect, albeit through multiple mechanisms of protection, particularly through up-regulation of growth factors that assist in repair of gastric tissue injuries.Mohd Fahami Nur Azlina Hj Mohd Saad Qodriyah Kien Hui Chua Yusof Kamisah 2017World Journal of Gastroenterology2017,23,32:8
17Recognition of motor imagery tasks for BCI using CSP and chaotic PSO twin SVM显示文摘Accurate modeling and recognition of the brain activity patterns for reliable communication and interaction are still a challenging task for the motor imagery(MI) brain-computer interface(BCI) system. In this paper, we propose a common spatial pattern(CSP) and chaotic particle swarm optimization(CPSO) twin support vector machine(TWSVM) scheme for classification of MI electroencephalography(EEG). The self-adaptive artifact removal and CSP were used to obtain the most distinguishable features. To improve the recognition results, CPSO was employed to tune the hyper-parameters of the TWSVM classifier. The usefulness of the proposed method was evaluated using the BCI competition IV-IIa dataset. The experimental results showed that the mean recognition accuracy of our proposed method was increased by 5.35%, 4.33%, 0.78%, 1.45%, and 9.26% compared with the CPSO support vector machine(SVM), particle swarm optimization(PSO) TWSVM, linear discriminant analysis(LDA), back propagation(BP) and probabilistic neural network(PNN), respectively. Furthermore, it achieved a faster or comparable central processing unit(CPU) running time over the traditional SVM methods.Li Duan Zhang Hongxin Muhammad Saad Khan Mi Fang 2017The Journal of China Universities of Posts and Telecommunications2017,24,3:7
18Duodenal variceal bleeding with large spontaneous portosystemic shunt treated with transjugular intrahepatic portosystemic shunt and embolization: A case report显示文摘BACKGROUND Duodenal variceal bleeding is a rare cause of gastrointestinal bleeding. The most common site is the duodenal bulb. It is usually detected endoscopically but it can be very challenging to diagnose if it is located distal to the second part od duodenum. The pre- transjugular intrahepatic portosystemic shunt (TIPS) presence of spontaneous portosystemic shunt (SPSS) was found to be associated with an increased risk of early morbidity and mortality after TIPS placement. CASE SUMMARY A 43-year-old cirrhotic male presented with melena for three days. Upper endoscopy was performed and showed active blood oozing from the distal duodenum concerning for ectopic duodenal varix. A computed tomography (CT) angiogram was performed and showed an enlarged cluster of venous collaterals around the distal duodenum. He underwent TIPS placement. He had another episode of melena three days later. Push enteroscopy with injection sclerotherapy into the duodenal varices was performed with no success. A repeat CT angiogram showed occluded TIPS shunt. Therefore, a TIPS revision was performed and there was an extensive portal venous thrombosis with a large shunt between the inferior mesenteric vein and left renal vein via the left gonadal vein. Thrombectomy and TIPS shunt balloon angioplasty was performed, followed by embolization of the portosystemic. The melena was resolved, and patient was discharged with arranged hepatology follow up. CONCLUSION It importance to look and embolize the SPSS shunts in patients with early TIPS dysfunction and recurrent duodenal variceal bleeding.Rohit Anand Saad Emhmed Ali Driss Raissi Wesam M Frandah 2019World Journal of Radiology2019,11,8:7
19Antimicrobial activity of mangrove plant(Lumnitzera littorea)显示文摘Objective:To investigate the antimicrobial activities of n-hexane.ethyl acetate and methanol extracts of the leaves of Lumnitzera littorea(L littorea) against six human pathogenic microbes. Methods:The antimicrobial activity was evaluated using disc diffusion and microdilution methods.Results:The antimicrobial activities of the crude extracts were increased with increasing the concentration.It is clear that n-hexane extract was the most effective extract. Additionally.Gram positive Bacillus cereus(B.cereus) appear to be the most sensitive strain while Pseudomonas aeruginosa(P.aeruginosa) and the yeast strains(Candida albicans(C. albicans) and Cryptococcus neoformans(C.neqformans)) appear to be resistance to the tested concentrations since no inhibition zone was observed.The inhibition of microbial growth at concentration as low as 0.04 mg/ml.indicated the potent antimicrobial activity of L littorea extracts.Conclusions:The obtained results are considered sufficient for further study to isolate the compounds responsible for the activity and suggesting the possibility of finding potent antibacterial agents from L littorea extracts.Shahbudin Saad Muhammad Taher Deny Susanti Haitham Qaralleh Nurul Afifah Binti Abdul Rahim 2011Asian Pacific Journal of Tropical Medicine2011,4,7:7
202株芽孢杆菌生物学特性研究及菌种鉴定显示文摘从发酵食品分离出的10株芽孢杆菌中,通过对其进行耐酸、耐胆盐筛选,选取了2株存活率最高的菌株513-A和513-O。对这2个菌株进行产酶定性试验并测定其产蛋白酶活力大小,再结合细菌形态学、生理生化特征和16S r DNA序列分析进行鉴定。结果显示,513-A为短小芽孢杆菌,513-O为Bacillus gobiensis。513-A菌株经p H值为2.0的HCl处理1 h存活率达70.61%,0.3%猪胆盐处理1 h存活率达67.30%;513-O菌株经p H值为2.0的HCl处理1 h存活率达59.43%,0.3%猪胆盐处理1 h存活率达87.50%。2株菌株均具有产纤维素酶和蛋白酶的能力,其产碱性蛋白酶活力大小分别为43.67,65.72 U/m L。由于短小芽孢杆菌具有提高饲料利用率和动物免疫力的功能,因此,513-A和513-O这2株菌株有作为动物饲料添加剂候选菌种的潜力,513-O还有作为产碱性蛋白酶菌种的潜力;且首次发现Bacillus gobiensis具有产纤维素酶和蛋白酶的功能。陈雅茜 樊溢 Saad Abdelrahman 王景雪 2018山西农业科学2018,46,2:6
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