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| 1 | Tyrosine kinase of insulin-like growth factor receptor as target for novel treatment and prevention strategies of colorectal cancer显示文摘AIM: To investigate the antineoplastic potency of the novel insulin-like growth factor 1 receptor (IGF-1R) tyro- sine kinase inhibitor (TKI) NVP-AEW541 in cell lines and primary cell cultures of human colorectal cancer (CRC). METHODS: Cells of primary colorectal carcinomas were from 8 patients. Immunostaining and crystal violet stain- ing were used for analysis of growth factor receptor pro- tein expression and detection of cell number changes, respectively. Cytotoxicity was determined by measuring the release of the cytoplasmic enzyme lactate dehydro- genase (LDH). The proportion of apoptotic cells was determined by quantifying the percentage of sub-G1 (hypodiploid) cells. Cell cycle status reflected by the DNA content of the nuclei was detected by flow cytometry. RESULTS: NVP-AEW541 dose-dependently inhibited the proliferation of colorectal carcinoma cell lines and primary cell cultures by inducing apoptosis and cell cycle arrest. Apoptosis was characterized by caspase-3 activa- tion and nuclear degradation. Cell cycle was arrested at the G1/S checkpoint. The NVP-AEW541-mediated cell cycle-related signaling involved the inactivation of Akt and extracellular signal-regulated kinase (ERK) 1/2, the upregulation of the cyclin-dependent kinase inhibitors p21Waf1/CIP1 and p27Kip1, and the downregulation of the cell cycle promoter cyclin D1. Moreover, BAX was upregu- lated during NVP-AEW541-induced apoptosis, whereas Bcl-2 was downregulated. Measurement of LDH release showed that the antineoplastic effect of NVP-AEW541 was not due to general cytotoxicity of the compound. However, augmented antineoplastic effects were ob-served in combination treatments of NVP-AEW541 with either 5-FU, or the EGFR-antibody cetuximab, or the HMG-CoA-reductase inhibitor fluvastatin. CONCLUSION: IGF-1R-TK inhibition is a promising novel approach for either mono- or combination treatment strategies of colorectal carcinoma and even for CRC che- moprevention. | Michael Hpfner Andreas P Sutter Alexander Huether Viola Baradari Hans Scherübl | 2006 | World Journal of Gastroenterology2006，12，35： | 10 |
| 2 | 中国推进消灭脊髓灰质炎终结战略计划技术问题:世界卫生组织专家解读显示文摘中国积极推进消灭脊髓灰质炎(脊灰)终结战略计划,分阶段将灭活脊灰病毒疫苗(Inactivated Poliovirus Vaccine,IPV)纳入免疫规划,预期2016年4月与全球同步停服三价(Trivalent)口服脊灰减毒活疫苗(Oral Poliomyelitis Attenuated Live Vaccine,t OPV),开始接种二价(Bivalent)OPV(b OPV)。为澄清对一些技术问题的认识,世界卫生组织驻中国代表处会同国内外相关专家,讨论了中国部分专业人员提出的主要技术和政策问题,包括停用OPV的原因,b OPV替代t OPV使用的步骤,转换前减少风险的准备等,形成专家解读,供相关部门和专业人员在推进消灭脊灰终结战略计划中参考。 | 左树岩 Lance Rodewald Roland Sutter Jackie Fournier-Caruana William Schluter Sergey Diorditsa | 2015 | 中国疫苗和免疫2015，21，4： | 9 |
| 3 | Neural stem cells, tumour stem cells and brain tumours: Dangerous relationships?显示文摘 | Sutter R Yadirgi G Marino S | 2007 | 中国神经肿瘤杂志2007，5，3： | 7 |
| 4 | Signaling pathways involved in the inhibition of epidermal growth factor receptor by erlotinib in hepatocellular cancer显示文摘瞄准:在肝细胞癌(HCC ) 检验导致 erlotinib 的生长抑制的内在的机制。方法:在基因表示的导致 Erlotinib 的改变用 cDNA 数组技术被评估;在蛋白质表示或蛋白质激活的变化用西方的弄污由于象 IGF-1-induced EGFR transactivation 一样的 erlotinib 治疗被调查。结果:Erlotinib 治疗禁止了 mitogen 激活和抄写(STAT ) 的激活的蛋白质(地图)-kinase 小径和信号变换器调停了表明哪个导致了调整由 cDNA 数组技术示威了的基因的 apoptosis 和房间周期的一个改变的表达式。象与象 Bcl-2, Bcl-X (L) 或 jun D 一样的 antiapoptotic 因素的一条下面规定联系的 caspases 和 gadds 一样的 proapoptotic 因素的 Overexpression 说明了让 erlotinib 的力量导致 apoptosis。支持 G1/S-transition 的房间周期管理者并且在 cyclin 依赖的激酶禁止者和 gadds 的表示上的 Downregulation 响应 erlotinib 贡献了 G1/G0-arrest 的正式就职。而且,我们显示了由 IGF-1-receptor 的调停 EGFR 的发信号的 transactivation 并且在受体受体十字谈话显示出 erlotinib 的禁止的效果。结论:我们的学习在 HCC 房间和 thus 使 EGFR-TK-inhibition 的行动的机制的理解清楚些可能便于 additively 或 synergistically 行动的联合治疗的设计。而且,我们对 erlotinib 处理作出回应的小径上的数据能在以后预言肿瘤的应答的海角到 EGFR-TKIs 是有用的。 | Alexander Huether Michael Hpfner Andreas P Sutter Viola Baradari Detlef Schuppan Hans Scherübl | 2006 | World Journal of Gastroenterology2006，12，32： | 7 |
| 5 | Intravitreal triamcinolone for diabetic macular edema that persists after laser treatment显示文摘 | Florian K.P. Sutter Judy M. Simpson Mark C. Gillies | 2004 | Ophthalmology2004，，11： | 3 |
| 6 | The fast m-transform:a fast computation of cross-correlations with binary m-sequence显示文摘 | Sutter E E | 1991 | SJAM J Comput1991，20，4： | 1 |
| 7 | The fast m - trasnform : a fast computation of cross - correlations with binary m - sequences 显示文摘 | E E Sutter | 1991 | Sian J Comput1991，20，： | 1 |
| 8 | Frequent K-ras mutations in small bowel adenocarcinomas显示文摘 | Sutter T Arber N Moss SF | 1996 | Dig Dis Sci1996，41，： | 1 |
| 9 | Fibrinogen and C-reactive protein on admission as markers of finaI infarct size atter primary angioplasty for acute myocardial infarction显示文摘 | De Buyzere M Gheeraert P | 2001 | Atheroselerosis2001，157，1： | 1 |
| 10 | Mapping of retinal function in diabetic retinaopathy using the multifocal electroretinogram显示文摘 | Palmowski AM Sutter EE Bearse MA | 1997 | Invest Ophthalmol Vis Sci1997，38，12： | 1 |
| 11 | A real-life prospective health economic study of elective single embryo transfer versus two-embryo transfer in first IVF/ICSI cycles显示文摘 | Gerris J De Sutter P De Neubourg D | 2004 | Hum Reprod2004，19，4： | 1 |
| 12 | Blockade of IGF-1 receptor tyrosine kinase has antineoplastic effects in hepatocellular carcinoma cells显示文摘 | Hopfner M Huether A Sutter AP | 2006 | Biochem Pharmacol2006，71，10： | 1 |
| 13 | Imaging visual function with the multifocal m-sequence technique显示文摘 | SUTTER E E | 2001 | Vision Research2001，41，1011： | 1 |
| 14 | Fluorescent insitu hybridization on human embryos showing cleavage arrest after freezing and thawing显示文摘 | Laverge H Van der Elst J De Sutter P | 1998 | Hum Reprod1998，13，： | 1 |
| 15 | An experimental technique for the measurement of temperature fields for the orthogonal cutting in high speed machining显示文摘 | Sutter G | 2003 | International Journal of Machine Tools & Manu- facture2003，43，7： | 1 |
| 16 | Treatment option for sperm-or oocyte-related fertilization failure :assisted oovyte activation following diagnositic heterologous ICSI显示文摘 | Heindryckx B Vander J Elst De Sutter P | 2005 | Hunman Reproduction2005，20，8： | 1 |
| 17 | The field topography of ERG components in man-I显示文摘 | Sutter E E Tran D | 1992 | The photopic Iuminance Response:Vis Res1992，32，： | 1 |
| 18 | Parameters controlling drug release from pellets coated with aqueous ethylcellulose dispersion 显示文摘 | LIPPOLD B H Sutter B K LIPPOLD B C | 1989 | Int J Pharm1989，54，1： | 1 |
| 19 | Hypoxia-induebile factor 1 alpha protein expression is controlled by oxygenregulated ubiquitination that is disrupted by deletions and missense mutations显示文摘 | Sutter CH Laughner E Semenza GL | 2000 | PNAS2000，97，9： | 1 |
| 20 | Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells显示文摘 | Stein E Koehn J Sutter W | 2008 | Wien Klin Wochenschr2008，120，34： | 1 |
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