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| 1 | Plecanatide-mediated activation of guanylate cyclase-C suppresses inflammation-induced colorectal carcinogenesis in Apc+/Min-FCCC mice显示文摘AIM To evaluate the effect of orally administered plecanatide on colorectal dysplasia in Apc^(+/Min-FCCC) mice with dextran sodium sulfate(DSS)-induced inflammation. METHODS Inflammation driven colorectal carcinogenesis was induced in Apc^(+/Min-FCCC) mice by administering DSS in their drinking water. Mice were fed a diet supplemented with plecanatide(0-20 ppm) and its effect on the multiplicity of histopathologically confirmed polypoid,flat and indeterminate dysplasia was evaluated. Plecanatide-mediated activation of guanylate cyclase-C(GC-C) signaling was assessed in colon tissues by measuring cyclic guanosine monophosphate(cG MP) by ELISA, protein kinase G-II and vasodilator stimulated phosphoprotein by immunoblotting. Ki-67, c-myc and cyclin D1 were used as markers of proliferation. Cellular levels and localization of b-catenin in colon tissues were assessed by immunoblotting and immunohistochemistry, respectively. Uroguanylin(UG) and GC-C transcript levels were measured by quantitative reverse transcription polymerase chain reaction(RT-PCR). A mouse cytokine array panel was used to detect cytokines in the supernatant of colon explant cultures. RESULTS Oral treatment of Apc^(+/Min-FCCC) mice with plecanatide produced a statistically significant reduction in the formation of inflammation-driven polypoid, flat and indeterminate dysplasias. This anti-carcinogenic activity of plecanatide was accompanied by activation of cG MP/GC-C signaling mediated inhibition of Wnt/b-catenin signaling and reduced proliferation. Plecanatide also decreased secretion of pro-inflammatory cytokines(IL-6, IL-1 TNF), chemokines(MIP-1, IP-10) and growth factors(GCSF and GMCSF) from colon explants derived from mice with acute DSS-induced inflammation. The effect of plecanatidemediated inhibition of inflammation/dysplasia on endogenous expression of UG and GC-C transcripts was measured in intestinal tissues. Although GC-C expression was not altered appreciably, a statistically significant increase in the level of UG transcripts was detected in the proximal small intestine and colon, potentially due to a reduction in intestinal inflammation and/or neoplasia. Taken together, these results suggest that reductions in endogenous UG, accompanied by dysregulation in GC-C signaling, may be an early event in inflammation-promoted colorectal neoplasia; an event that can potentially be ameliorated by prophylactic intervention with plecanatide.CONCLUSION This study provides the first evidence that orally administered plecanatide reduces the multiplicity of inflammation-driven colonic dysplasia in mice, demonstrating the utility for developing GC-C agonists as chemopreventive agents. | Wen-Chi L Chang Shet Masih Anusha Thadi Viren Patwa Apoorva Joshi Harry S Cooper Vaseem A Palejwala Margie L Clapper Kunwar Shailubhai | 2017 | World Journal of Gastrointestinal Pharmacology and Therapeutics2017，8，1： | 5 |
| 2 | Metastatic clear cell variant of hepatocellular carcinoma with an occult hepatic primary显示文摘Metastatic clear cell carcinomas are commonly seen in the kidney and lung. Clear cell variant of hepatocellular carci- noma is an uncommon tumour. Diagnosis is usually made by correlation of histopathology. tumour markers and im- munohistochemistry, especially HepPar 1. In this unusual case of metastatic clear cell carcinoma presenting as Sister Mary Joseph’s nodule, no primary evidence was observed radiologically in the liver, but the level of alfa fetoprotein was markedly elevated. Metastatic clear cell carcinoma of the liver with an occult hepatic primary was diagnosed by immunohistochemical profile of the tumour. | Samir ShahSamir Shah Sudeep Gupta Tanuja Shet Amita Maheshwari Rekha Wuntkal and Kunisshery Mallath Mohandas | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，2： | 3 |
| 3 | Prevalence of respiratory syncytial virus infection among children hospitalized with acute lower respiratory tract infections in Southern India显示文摘BACKGROUND Respiratory syncytial virus(RSV) is a leading cause of lower respiratory infections among children.AIM To investigate the proportion of RSV and non-RSV respiratory viral infections among hospitalized children ≤ 5 years.METHODS Hospitalized children aged < 5 years, with a diagnosis of acute lower respiratory infections(ALRI), admitted between August 2011-August 2013, were included.Cases were defined as laboratory-confirmed RSV and non-RSV respiratory viruses by direct fluorescence assay from the nasopharyngeal wash.RESULTS Of 383 1-59 mo old children hospitalized with an acute lower respiratory infection, 33.9%(130/383) had evidence of viral infection, and RSV was detected in 24.5%(94/383). Co-infections with RSV and other respiratory viruses(influenza A or B, adenovirus, para influenza 1, 2 or 3) were seen in children 5.5%(21/383). Over 90% of the RSV-positive children were under 2 years of age. RSV was detected throughout the year with peaks seen after the monsoon season.Children hospitalized with RSV infection were more likely to have been exposed to a shorter duration of breastfeeding of less than 3 mo. RSV positive children had a shorter hospital stay, although there were significant complications requiring intensive care. Use of antibiotics was high among those with RSV and non-RSV viral infections.CONCLUSION Our study provides evidence of a high proportion of RSV and other virusassociated ALRI among hospitalized children in India. RSV infection was associated with fewer days of hospital stay compared to other causes of lower respiratory infections. A high level of antibiotic use was seen among all respiratory virus-associated hospitalizations. These results suggest the need for implementing routine diagnostics for respiratory pathogens in order to minimize the use of unnecessary antibiotics and plan prevention strategies among pediatric populations. | Sandesh Kini Bhuvanesh Sukhlal Kalal Sara Chandy Ranjani Shamsundar Anita Shet | 2019 | World Journal of Clinical Pediatrics2019，8，2： | 3 |
| 4 | Efficacy of dietary phytase supplementation on laying performance and expression of osteopontin and calbindin genes in eggshell gland显示文摘This study was conducted to evaluate the effects of different levels of dietary phytase supplementation in the layer feed on egg production performance,egg shell quality and expression of osteopontin(OPN) and calbindin(CALB1) genes.Seventy-five White Leghorn layers at 23 weeks of age were randomly divided into 5 groups consisting of a control diet with 0.33% non-phytate phosphorus(NPP) and 4 low phosphorus(P) diets: 2 diets(T1 and T2) with 0.24% NPP t 250 FTU/kg laboratory produced phytase or commercial phytase and another 2 diets(T3 and T4) with 0.16% NPP t 500 FTU/kg laboratory produced phytase or commercial phytase with complete replacement of inorganic P.The results indicated that there were no significant differences(P > 0.05) in egg production performance and quality of egg during the first 2 months of trial.However,in next 2 months,a significant drop in egg production and feed intake was observed in birds fed diets with low P and 500 FTU/kg supplementation of laboratory produced phytase.Osteopontin gene was up-regulated whereas the CALB1 gene was down regulated in all phytase treatment groups irrespective of the source of phytase.The current data demonstrated that250 FTU/kg supplementation of laboratory produced phytase with 50% less NPP supplementation and500 FTU/kg supplementation of commercial phytase even without NPP in diet can maintain the egg production.The up-regulation of OPN and down regulation of CALB1 in egg shell gland in the entire phytase treated group birds irrespective of the source of enzymes is indicative of the changes in P bioavailability at this site. | Divya Shet Jyotirmoy Ghosh Sreeja Ajith Vaibhav B.Awachat Arumbackam V.Elangovan | 2018 | Animal Nutrition2018，，1： | 2 |
| 5 | Finite element analysis of the orthogonal metal cutting process显示文摘 | Shet C DENG Xiao-min | 2000 | Journal of Materials Processing Technology2000，105，1： | 1 |
| 6 | A technique to immobilize bioactive proteins, including bone morphogenetic protein-4 (BMP-4) on titanium alloy显示文摘 | Puleo DA Kissling RA Shet MS | 2002 | Biomaterials2002，23，9： | 1 |
| 7 | Analysis of Energy Balance when Using Cohesive Zone Models to Simulate fracture processes显示文摘 | Shet C Chandra N | 2002 | ASME Journal of Engineering Material and Technology2002，，124： | 1 |
| 8 | Splenic angiomatoid nodular transformation in child with inflammatory pseudotumor- like areas 显示文摘 | Vyas M Deshmukh M Shet T | 2011 | Indian J Pathol Microbiol2011，54，4： | 1 |
| 9 | Digital Converter for Differential Capacitive Sensors 显示文摘 | Mohan N M Shet A R Kedamath S | 2008 | IEEE Transactions on Instrumentation and Measurement(S0018-9456)2008，57，11： | 1 |
| 10 | Mechanical behaviour of function alised nanotubes 显示文摘 | S Namitae N Chandra C Shet | 2004 | Chemical Physics Letters2004，387，： | 1 |
| 11 | Liver biopsy显示文摘 | Bravo AA Shet h SG Chopra S | 2001 | N EngMed2001，34，4： | 1 |
| 12 | Carrier concen- tration tuning of bandgap-reduced p-type ZnO films by codoping of Cu and Ga for improving photoelectro- chemical response显示文摘 | SHET S AHN K S YAN Y | 2008 | Journal of Applied Physics2008，103，07： | 1 |
| 13 | A finite element study of the effect of friction in orthogonal metal cutting 显示文摘 | Guoqin Shi Xiaomin Deng Chandrakanth Shet | 2002 | Finite Elements in Analysis and Design2002，38，： | 1 |
| 14 | Induction of micropartiele and cell-associated intravascular tissue factor in human endotoxemia显示文摘 | Aras O Shet A Bach RR | 2004 | Blood2004，103，12： | 1 |
| 15 | Finite elementanalysis of the orthogonal metal cutting process显示文摘 | Shet C Deng Xiaomin | 2000 | Journal of Materials Processing Technology2000，105，12： | 1 |
| 16 | Clinicopathologic study and outcome analysis of thyroid lymphomas : experience from a tertiary cancer center显示文摘 | Katna R Shet T Sengar M | 2013 | Head Neck2013，35，2： | 1 |
| 17 | Residual stresses and strains in orthogonal metal cutting 显示文摘 | Shet C Deng X | 2003 | International Journal of Machine Tools & Manufacture2003，43，： | 1 |
| 18 | The cytological diagnosis of paediatric renal tumours显示文摘 | Shet T Viswanathan S | 2009 | J Clin Pathol2009，62，11： | 1 |
| 19 | Humoral immune responses to peri- odontal pathogens in the elderly 显示文摘 | Shet U Oh HK Chung H J | 2015 | J Periodontal Implant Sci2015，45，5： | 1 |
| 20 | Neonatal & maternal group B streptococcal infections : A comprehensive review 显示文摘 | SHET A FERRIERI P | 2004 | Indian J Med Res2004，120，： | 1 |
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